Microbial populations analysis and field application of biofilter for the removal of volatile-sulfur compounds from swine wastewater treatment system

A biofilter packed with granular activated carbon (GAC) was applied to eliminate volatile-sulfur compounds (VSC) emitted from solid-liquid separation tank in swine wastewater treatment system. Hydrogen sulfide, methanethiol, dimethyl disulfide, and dimethyl sulfide were effectively reduced to 96-100% at gas residence times of 13-30s. Elemental sulfur and sulfate are their primary oxidation metabolites. Regarding odor, an average of 86% reduction was achieved at short residence time (13s). In addition, bioaerosol emissions could also be effectively reduced by 90% with the biofilter. Advantages of the system include low moisture demand, low pressure drop, and high biofilm stability. Further characterization of bacterial populations of the activated carbon samples using the fluorescent in situ hybridization (FISH) technique revealed that Pseudomonas sp. remained the predominant community (56-70%) after long-term evaluation of 415 days.     

Using UV pretreatment to enhance biofiltration of mixtures of aromatic VOCs

Mixtures of airborne toluene and o-xylene, two relatively recalcitrant volatile organic compounds (VOCs), were treated effectively using integrated UV-biofiltration. The set-up consisted of a biofilter receiving UV-pretreated stream and a reference biofilter receiving no pretreatment. Experimental conditions included UV fluences of 6 and 12 mJcm(-2) as well as air flow rates of 6.3 and 9.4 Lmin(-1), corresponding to biofilter empty bed retention times (EBRTs) of 45 and 30s, respectively. The inlet concentration of organics (toluene and o-xylene) ranged between 70 and 650 mg(carbon)m(-3). The UV-biofilter consistently provided removal efficiencies of greater than 95% over the range of toluene and o-xylene inlet concentrations. Also, the coupled UV-biofiltration system provided up to 60% additional contaminant removal compared to the sum of that offered by UV and reference biofilter, demonstrating the synergistic effect of UV on biofilter performance. The UV photooxidation partially oxidized a fraction of toluene and o-xylene into water soluble and more biodegradable intermediates, such as acetaldehyde and formaldehyde, which were readily removed in the downstream biofilter. These intermediates along with up to 20ppmv ozone, formed through the photolysis of oxygen by 185 nm UV, contributed to the enhanced degradation of parent VOCs in the biofilter as well as the absence of any inhibitory effects of the VOCs on one another. Also, the presence of ozone helped control the growth of excess biofilm in the UV-coupled biofilter. While the standalone biofilter showed significant pressure drop increase (of up to 14 mm H(2)Om(-1) of the bed) over the course of experiment, the UV-coupled biofilter maintained a relatively low pressure drop of less than 3 mmH(2)Om(-1) of the bed.     

Ethylene removal evaluation and bacterial community analysis of vermicompost as biofilter material

Biofiltration of ethylene provides an environmentally friendly and economically beneficial option relative to physical/chemical removal, where selection of appropriate bed material is crucial. Here the vermicompost with indigenous microorganisms as bed material was evaluated for ethylene removal through batch test and biofilter experiment. Temporal and spatial dynamics of bacterial community in the vermicompost-biofilter under different ethylene loads were characterized by culture and denaturing gradient gel electrophoresis (DGGE) methods. The results showed that ethylene was effectively degraded by the vermicompost under conditions of 25-50% moisture content and 25-35 °C temperature. The vermicompost-biofilter achieved nearly 100% ethylene removal up to an inlet load of 11 mg m⁻³ h⁻¹. Local nitrogen lack of the vermicompost in the biofilter was observed over operation time, but the change of pH was slight. DGGE analysis demonstrated that the bacterial abundance and community structure of vermicompost-biofilter varied with the height of biofilter under different ethylene loads. Pseudomonads and Actinobacteria were predominant in the biofilter throughout the whole experiment.     

Kinetics of the removal of mono-chlorobenzene vapour from waste gases using a trickle bed air biofilter

The performance of a trickle bed air biofilter (TBAB) in the removal of mono-chlorobenzene (MCB) was evaluated in concentrations varying from 0.133 to 7.187 g m(-3) and at empty bed residence time (EBRT) varying from 37.7 to 188.52 s. More than 90% removal efficiency in the trickle bed air biofilter was achieved for the inlet MCB concentration up to 1.069 g m(-3) and EBRT less than 94.26 s. The trickle bed air biofilter was constructed with coal packing material, inoculated with a mixed consortium of activated sludge obtained from sewage treatment plant. The continuous performance of the removal of MCB in the trickle bed air biofilter was monitored for various gas concentrations, gas flow rates, and empty bed residence time. The experiment was conducted for a period of 75 days. The trickle bed air biofilter degrading MCB with an average elimination capacity of 80 g m(-3) h(-1) was obtained. The effect of starvation was also studied. After starvation period of 8 days, the degradation was low but recovered within a short period of time. Using macrokinetic determination method, the Michaelis-Menten kinetic constant K(m) and maximum reaction rate, r(max) evaluated as 0.121 g m(-3) s(-1) and 7.45 g m(-3), respectively.     

Removal of p-xylene from an air stream in a hybrid biofilter

Biofiltration of an air stream containing p-xylene has been studied in a laboratory hybrid biofilter packed with a mixture of mature pig compost, forest soil and the packing material which was made of polyethylene (PE) and used in the moving bed biological reactor (MBBR) in wastewater treatment. Three flow rates, 9.17, 19.87 and 40.66 m(3)m(-2)h(-1), were investigated for p-xylene inlet concentration ranging from 0.1 to 3.3 g m(-3). A high elimination capacity of 80 g m(-3)h(-1) corresponding to removal efficiency of 96% was obtained at a flow rate of 9.17 m(3)m(-2)h(-1) (empty bed residence time of 132 s). At a flow rate of 40.66 m(3)m(-2)h(-1) (empty bed residence time of 30s), the maximum elimination capacity for p-xylene was 40 g m(-3)h(-1) and removal efficiencies were in the range of 47-100%. The production of carbon dioxide (P(CO(2))) is proportional to elimination capacity (EC) and the linear relation was formulated as P(CO(2))=1.65EC+15.58. Stable pH values ranging from 6.3 to 7.6 and low pressure drop values less than 0.2 cm H(2)O (19.6 Pa) of packing media in compost-based biofilter of hybrid biofilter were observed, which avoided acidification and compaction of packing media and sustained the activity of microorganism populations.     

Combined removal of BTEX in air stream by using mixture of sugar cane bagasse, compost and GAC as biofilter media

Biofiltration of air stream containing mixture of benzene, toluene, ethyl benzene and o-xylene (BTEX) has been studied in a lab-scale biofilter packed with a mixture of compost, sugar cane bagasse and granulated activated carbon (GAC) in the ratio 55:30:15 by weight. Microbial acclimation was achieved in 30 days by exposing the system to average BTEX inlet concentration of 0.4194 gm(-3) at an empty bed residence time (EBRT) of 2.3 min. Biofilter achieved maximum removal efficiency more than 99% of all four compounds for throughout its operation at an EBRT of 2.3 min for an inlet concentration of 0.681 gm(-3), which is quite significance than the values reported in the literature. The results indicate that when the influent BTEX loadings were less than 68 gm(-3)h(-1) in the biofilter, nearly 100% removal could be achieved. A maximum elimination capacity (EC) of 83.65 gm(-3)h(-1) of the biofilter was obtained at inlet BTEX load of 126.5 gm(-3)h(-1) in phase IV. Elimination capacities of BTEX increased with the increase in influent VOC loading, but an opposite trend was observed for the removal efficiency. The production of CO(2) in each phase (gm(-3)h(-1)) was also observed at steady state (i.e. at maximum removal efficiency). Moreover, the high concentrations of nitrogen in the nutrient solution may adversely affect the microbial activity possibly due to the presence of high salt concentrations. Furthermore, an attempt was also made to isolate the most profusely grown BTEX-degrading strain. A Gram-positive strain had a high BTEX-degrading activity and was identified as Bacillus sphaericus by taxonomical analysis, biochemical tests and 16S rDNA gene analysis methods.     

Performance of low pH biofilters treating a paint solvent mixture: continuous and intermittent loading

Two biofilters packed with a reticulated polyurethane foam medium were inoculated with a compost-derived enrichment culture grown under acidic conditions (pH 3.0) and then operated over a period lasting 63 days. Both biofilters were supplied with a humidified gas stream containing a five-component mixture of acetone, methyl ethyl ketone, toluene, ethylbenzene, and p-xylene at a total VOC loading rate 80.3 gm(-3)h(-1) to simulate treatment of air emissions resulting from manufacture of reformulated paint. One biofilter was operated under continuous loading conditions and the other received intermittent loading with contaminants supplied only 8 h/day. Nutrient solution with pH 3.0 was supplied approximately once per week to provide nitrogen and other nutrients. Data are presented which demonstrate that undefined mixed cultures acclimated at low pH can successfully treat paint solvent mixtures in biofilters. The biofilter receiving continuous loading reached high overall removal efficiency (greater than 90% overall removal) 3 weeks after startup, and performance increased over time reaching overall removal in the range of 97-99% after 50 days. Performance of the intermittently loaded biofilter developed more slowly, requiring 6 weeks to stabilize at an overall removal efficiency in excess of 90%. In both biofilters, ketone components were more rapidly degraded than aromatic components, and removal of aromatic compounds was somewhat unstable even after 2 months of biofilter operation. Scanning electron microscopy (SEM) revealed that fungi dominated the microbial populations in both biofilters.     

生物气溶胶监测仪的校准方法比较

生物气溶胶监测仪利用荧光光谱法检测空气中的生物性物质,对疾病防控以及生物防恐预警意义重大.研究发现目前的国产生物气溶胶监测仪在采集空气中的生物颗粒时并不能区分不同细菌状态,得到的为总细菌数.通过实时定量PCR法和菌落计数法检测BioSampler采集瓶中的微生物,发现总细菌数和可培养的细菌数差异显著.利用扫描电子显微镜...     

High loading toluene treatment in a compost based biofilter using up-flow and down-flow swing operation

A compost/ceramic (1:1, v/v) three section laboratory-scale biofilter inoculated with acclimated activated sludge was examined to treat high loading toluene vapors from a synthetic gas stream. The biofilter was operated continuously at different gas flow rates, 0.108-0.15m(3)h(-1), with inlet toluene concentrations ranging 0.5-13gm(-3). The overall performance of the biofilter was divided to seven stages according to the mode of operation (down-flow and up-flow) over a period of 102 days. Removal efficiencies ranging from 48 to 100% and elimination capacities ranging from 26 to 180gm(-3)h(-1) were observed depending on the initial loading rates and the mode of operations. A maximum elimination capacity of 180gm(-3)h(-1) was observed in the last period at an inlet toluene concentration of about 13gm(-3). The results showed that changing the mode of operation (up-flow and down-flow) periodically will improve the performance of the biofilter under high inlet toluene concentration (higher than 4gm(-3)). Results obtained in this study provide insight into the possibility of the biofilter to treat high inlet concentrations rather than low concentrations well known in the literature.     

生物气溶胶雾化器对大肠杆菌活性影响的评价

生物气溶胶的研究依赖于稳定可靠的雾化过程。从颗粒数量浓度、粒径大小分布、细菌可培养性和细胞膜破损等方面评价不同气溶胶发生器的性能, 并探讨进气流量的影响。结果表明在气溶胶发生过程中的机械应力严重损害细胞膜的完整性,使得细菌的可培养率降低90%以上,并且随着进气流量的增加,细胞碎片的比例增加,细胞膜损伤更为严重。由于生物气溶胶的研究不仅需要高浓度的微生物,更要保证细胞的完整性和可培养性,因此以上研究将对生物气溶胶发生器的性能评价和选择提供依据。     

Biofiltration of wastewater lift station emissions: evaluation of VOC removal in the presence of H2S

The capacity of biofilter systems to remove volatile organic compounds in the presence of high concentrations of hydrogen sulfide was investigated for applications in wastewater lift stations. The treatment system was an enclosed unit composed of a biotrickling filter coupled with a biofilter. The biofilter media were plastic hollow spherical balls filled with a compost mixture; and the biotrickling filter media was a structured plastic packing. The gases from the pumping station wet well were a mixture of H₂S and low concentration aliphatic and aromatic VOCs, toluene being the most significant in concentrations of 41 ppb. The H₂S concentration was 314 ppm with fluctuations of 100 ppm resulting from pumping cycles at the station. No inhibition effect was detected from the simultaneous biological removal of VOCs and H₂S: toluene removal efficiency was 91% with the two sections contributing approximately equally to the pollutant removal; and the average removal of H₂S was 74%. A traditional open-in-ground biofilter filled with wood chips and compost, existing in the site, attained similar removal efficiencies for toluene, but the elimination capacity of the biotrickling/biofilter system was 3.3-times higher than the open biofilter.     

Trimethylamine (TMA) biofiltration and transformation in biofilters

Bioremoval of trimethylamine (TMA) in two three-stage biofilters packed with compost (A) and sludge (B), respectively, was investigated. Both biofilters were operated with an influent TMA concentration of 19.2-57.2mgm(-3) for 67 days. Results showed that all of the inlet TMA could be removed by both biofilters. However, removal efficiency and transformation of TMA in each section of both biofilters was different. In the Introduction section, TMA removal efficiency and maximum elimination capacity of the compost medium were greater than those of sludge medium under higher inlet TMA concentration. In comparison with biofilter A, considerably higher NH(3) concentrations in effluent of all three sections in biofilter B were observed after day 19. Although, NO(2)(-)-N concentration in each section of biofilter A was relatively lower, NO(3)(-)-N content in each section of biofilter A increased after day 26, especially in the Materials and method section which increased remarkably due to a lesser amount of TMA and higher ammonia oxidation and nitrification in compost medium. In contrast, neither NO(2)(-)-N nor NO(3)(-)-N were detected in either section of biofilter B at any time throughout the course of the experiment. The cumulative results indicated that compost is more favorable for the growth of TMA-degrading and nitrifying bacteria as compared to the sludge and could be a highly suitable packing material for biodegradation and transformation of TMA.     

A miniature biochip system for detection of aerosolized Bacillus globigii spores

The feasibility of using a novel detection scheme for the analysis of biological warfare agents is demonstrated using Bacillus globigii spores, a surrogate species for Bacillus anthracis. In this paper, a sensitive and selective enzyme-linked immunosorbent assay using a novel fluorogenic alkaline phosphatase substrate (dimethylacridinone phosphate) is combined with a compact biochip detection system, which includes a miniature diode laser for excitation. Detection of aerosolized spores was achieved by coupling the miniature system to a portable bioaerosol sampler, and the performance of the antibody-based recognition and enzyme amplification method was evaluated. The bioassay performance was found to be compatible with the air sampling device, and the enzymatic amplification was found to be an attractive amplification method for detection of low spore concentrations. The combined portable bioaerosol sampler and miniature biochip system detected 100 B. globigii spores, corresponding to 17 aerosolized spores/L of air. Moreover, the incorporation of the miniature diode laser with the self-contained biochip design allows for a compact system that is readily adaptable to field use. In addition, these studies have included investigations into the tradeoff between assay time and sensitivity.     

A comparative assessment of biofiltration and activated sludge diffusion for odour abatement

The deodorization performance of a biofilter and an activated sludge diffusion (AS) system was comparatively evaluated in terms of removal efficiency (RE) and process stability at empty bed residence times (EBRT) ranging from 94 to 32s. Both bioreactors were fed with a synthetic odorous emission containing H(2)S, butanone and toluene at 23.6-43.3, 4.3-6.3 and 0.4-0.6 mg m(-3), respectively. While the outlet H(2)S concentration was always lower than 1.4 mg m(-3), the REs for butanone and toluene remained higher than 95% in both bioreactors regardless of the EBRT. The continuous supply of wastewater in the AS unit did not affect removal and appeared to be a requirement for efficient pollutant abatement. Despite the narrow carbon source spectrum treated, the AS system maintained a large bacterial diversity over time. Therefore, the results obtained confirmed the potential of AS systems as a robust and efficient biotechnology for odour treatment in WWTPs.     

Influence of air inlet and cleaning chamber on the performance of mining cartridge filter

This study is aimed at shedding light on the influence laws of air inlet position, air inlet area and cleaning chamber. To achieve this aim, first, the influences of air inlet position on pressure drop, air flow rate and mass of coal dust collected on the filter cartridges were investigated with the aid of a homemade experimental system. In addition, the effects of air inlet area on dynamic pressure and air flow rate of the air inlet were evaluated. Furthermore, the velocity within 30 cm in front of the air inlet was tested for determining the effective suction range. Finally, the impacts of cleaning chamber height on pressure drop and air flow rate of the cartridge filter were demonstrated. The research is expected to provide guidance for the setting of the air inlet and the cleaning chamber of the cartridge filter.     

不同环境参数对间冷式冰箱蒸发器结霜换热性能的影响

文章对一个典型间冷式冰箱的冷藏室蒸发器在不同的环境参数下进行了结霜工况的实验研究,研究了空气流速、空气相对湿度、制冷剂流量等对蒸发器性能的影响。考察时间为6～8小时,与实际应用相符,给出了换热量、空气侧压降、结霜量在结霜过程中的动态变化规律。结果表明不同的环境参数对结霜的影响差别较大。在各参数随时问的变化中,空气侧压降呈指数关系增长,结霜量呈直线关系增长。     

Collection of nonpolar organic compounds from ambient air using polyurethane foam-granular adsorbent sandwich cartridges.

Glass cartridges containing 6-10 g of Tenax-GC or 15 g of XAD-2 resin packed between two slices of polyurethane foam (PUF) were used in a General Metal Works PS-1 high-volume sampler to collect chlorobenzenes (CBs) containing three to six chlorine atoms, hexachlorocyclohexanes (HCHs), and two-ring aromatic hydrocarbons from 35-385 m3 of air. Laboratory experiments were run by vaporizing known quantities of analytes into a clean airstream for sampling by the PS-1 system at 20 degrees C. Collection efficiencies, determined from mass balance of the quantities introduced and recovered, ranged from 70 to 120% for individual compounds and averaged 93% overall. Penetration of analytes to backup adsorbent traps showed an inverse correlation to vapor pressure. The method was used to collect the above compounds from urban and rural air.     

Biofiltration of volatile ethanol using sugar cane bagasse inoculated with Candida utilis.

Candida utilis (C. utilis) growing on sugar cane bagasse complemented with a mineral salt solution was studied for gaseous ethanol removal in a biofilter. Ethanol loads from 93.7 to 511.9 g/h m(3) were used, by varying both inlet ethanol concentration (9.72 to 52.4 g/m(3)) and air flow rate (1.59 x 10(-3) to 2.86 x 10(-3) m(3)/h). At a loading rate of 93.7 g/h m(3), a steady-state was maintained for 300 h. Ethanol removal was complete, and 76.3% of the carbon consumed was found in carbon dioxide. At an higher aeration rate (ethanol load=153.8 g/h m(3)), the biofilter displayed an average removal efficiency (RE) of 70%, and an elimination capacity (EC) of 107.7 g/h m(3). Only 64.4% of the carbon consumed was used for CO(2) production. Acetaldehyde and ethyl acetate in the outlet gas attained 7.86 and 20.4% in terms of carbon balance, respectively. In both cases, the transient phase was less than one day. At a high inlet ethanol concentration (52.4 g/m(3)), no steady-state was observed and the process stopped during the third day. In the three cases, final biomass was poor, ranging from 10.5 to 14.8 mg/g dm. Final pH 4.0-4.6, indicated that acidifying non-volatile metabolites, such as acetate, accumulated in the reactor.     

Evaluating the impact of water supply strategies on p-xylene biodegradation performance in an organic media-based biofilter

The influence of water irrigation on both the long-term and short-term performance of p-xylene biodegradation under several organic loading scenarios was investigated using an organic packing material composed of pelletised sawdust and pig manure. Process operation in a modular biofilter, using no external water supply other than the moisture from the saturated inlet air stream, showed poor p-xylene abatement efficiencies (≈33 ± 7%), while sustained irrigation every 25 days rendered a high removal efficiency (RE) for a critical loading rate of 120 g m(-3)h(-1). Periodic profiles of removal efficiency, temperature and moisture content were recorded throughout the biofilter column subsequent to each biofilter irrigation. Hence, higher p-xylene biodegradation rates were always initially recorded in the upper module, which resulted in a subsequent increase in temperature and a decrease in moisture content. This decrease in the moisture content in the upper module resulted in a higher removal rate in the middle module, while the moisture level in the lower module steadily increased as a result of water condensation. Based on these results, mass balance calculations performed using measured bed temperatures and relatively humidity values were successfully used to account for water balances in the biofilter over time. Finally, the absence of bed compaction after 550 days of continuous operation confirmed the suitability of this organic material for biofiltration processes.     

Effect of hypersaline aniline-containing pharmaceutical wastewater on the structure of activated sludge-derived bacterial community

In order to explore the effect of hypersaline aniline-containing pharmaceutical wastewater on the structure of activated sludge-derived bacterial community, RAPD-PCR and PCR-DGGE techniques were used to analyze the change of bacterial community diversity and structure during the acclimation to different concentrations of two types of wastewater. Different bacterial community structures and significant shift in the bacterial community diversity were observed during the acclimation of wastewater. The number of bacterial species and the abundance of bacteria acclimated to different concentration of wastewater decreased when the concentration increased. The trends of bacterial community diversity indices revealed by RAPD or DGGE profiles were different. The change of bacterial community diversity might be due to the adaptation and mutation under selection issues in the acclimation of wastewater. The relationship between diversity indices and the content of chemical oxygen demand (COD), chloride, salinity and aniline based on RAPD and DGGE data indicated that the change of the bacterial community diversity might not be due to a single factor but might be due to the integrated effects of all the contaminants in the wastewater. Phylogenic trees based on RAPD and DGGE fingerprints were different, indicating that the effect of wastewaters A and B on the bacterial community structure was different, which might be due to the characteristics of wastewater.