^99mTc—MIBI制备反应热力学研究

报道了以MIBI冻干药盒制备~(99m)Tc—MIBI的反应热力学研究。结果表明,~(99m)Tc—MIBI的制备反应为吸热反应,反应热ΔH为339.2kJ/mol,升高反应温度时,~(99m)Tc—MIBI的标记率也提高。为使~(99m)Tc—MIBI的标记率大于90％,反应温度必须高于77℃。     

^99mTc—MIBI心肌显像对急性心肌梗塞溶栓治疗的评价

２０例急性心肌梗塞患者（其中溶栓再灌注组１２例，无再灌注组８例）在溶栓前及发病后５－７天行＾９９ｍＴｃ－ＭＩＢＩ心肌显像，观察心肌缺血的状况，结果表明，尿激酶在发病６ｈ内给药者，２ｈ血管再通率为６６．７％，发病６－１２ｈ内给药者，２ｈ血管再通率为５０％，再灌注组和无再灌注线溶栓前心肌显像心肌缺损得分无显著差异，再灌注缚溶栓后心肌得分显著低于溶栓前（Ｐ〈０．０１）。缺损得分减少７０．２±１０．８％，     

^99mTc—MIBI显像鉴别甲状腺“冷”结节的性质

用＾９９ｍＴｃ－ＭＩＢＩ显像法对５６例经病理证实的甲状腺“冷”结节患者鉴别，结果血流丰富的共２２例，＾９９ｍＴｃ－ＭＩＢＩ显像Ｔ／ＮＴ比值，恶性病变中乳头状癌、滤泡状癌，髓样癌，未分化癌均与＾９９ｍＴｃＯ＾－５显像有非常显著性差异，而良性病变中仅腺瘤有显著性差异，其余均无显著性差异。     

应用放射自显影术观察^99mTc—MIBI在大鼠甲甲状腺内的动态分布

报道了＾９９ｍＴｃ－ＭＩＢＩ在大鼠甲状腺内的动态分布。结果显示,甲状腺摄取＾９９ｍＴｃ－ＭＩＢＩ高峰在注药后３－６０ｍｉｎ,其前３ｍｉｎ主要与血供有关。１５－６０ｍｉｎ是腺体摄取的主要过程,为浓聚滞留时间,注药后６０ｍｉｎ内单位重量甲状腺组织摄取放射性大于同时间其它脏器和组织。放射自显影结果显示,在;甲状腺滤泡细胞周过部即上皮细胞及其周围银颗粒分布致密,而在滤泡腔内的胶质和滤泡间质内很少或基本未见     

^99mTc—MIBI评价缺血／再灌注心肌活力的实验研究

应用＾９９ｍＴＣ－ＭＩＢＩ对家兔缺血／再灌注心肌细胞及线粒体代谢和活力进行了评价，将家兔ＬＡＤ阻断２０ｍｉｎ，３ｈ再灌主，于灌注后２－５ｍｉｎ静脉注射＾９９ｍＴｃ－ＭＩＢＩ，结果缺血３ｈ组缺血心肌再灌注早期（１０ｍｉｎ）晚期（３ｈ）相对放射性活度，心肌ＡＴＰ含量均明显低于缺前２０ｍｉｎ组，非缺血心肌和缺血２０ｍｉｎ心肌＾９９ｍＴｃ－ＭＩＢＩ亚细胞分布与ＳＤＨ活性呈显著正相关（ｒ＝０．８８，Ｐ〈０．     

^99Tc^m—MIBI肿瘤阳性显像的临床应用

探讨了^99Tc^m-MIBI肿瘤阳性显像在多种恶性肿瘤临床诊断中的应用价值。静脉注射^99Tc^m-MIBI 15～30min后,用美国GE公司SPX-6型SPECT行病变局部平面和断层显像,计算肿瘤与本底比值（T/B）,当T/B≥1.5时判定显像结果为阳性;肝脏肿瘤诊断则比较胶体显像和阳性显像时肿瘤部位是否有填充,若有填充则为阳性。21例受检者中病理诊断为恶性肿瘤17例,良性肿瘤4例。17例恶性肿瘤^99Tc^m-MIBI阳性显像15例阳性,2例阴性;4例良性肿瘤患者显像结果均为阴性。本组病例^99Tc^m-MIBI阳性显像的敏感性为88%,特异性为100%,阳性预测值为100%,阴性预测值为66.7%,准确率为90%。结果表明^99Tc^m-MIBI阳性显像诊断和鉴别诊断良恶性肿瘤具有良好的临床应用前景。     

^99mTc—MIBI SPECT与^18F—FDG PET诊断胶质瘤复发及恶变对比分析

对２８例经过治疗的胶质瘤患者进行Ｇｄ－ＤＴＰＡＭＲＩ和＾１８Ｆ－ＦＤＧＰＥＴ显像，其中１４例同时进行＾９９ｍＴｃ－ＭＩＢＩＳＰＥＣＴ显像，并与治疗前解剖影像对比，ＰＥＴ结果发现１４例高代谢，１０例低代谢，４例瘤灶中心坏死，周边ＦＤＧ环状摄取，８例首次手术病理Ｉ－Ⅱ级星形细胞瘤患者中５例为高代谢，＾１８Ｆ－ＦＤＧＰＥＴ和＾９９ｍＴＣ－ＭＩＢＩＳＰＥＣＴ诊断胶质瘤复发及恶变的灵敏度依次为９３．３％，９     

Kinetic study on Iigand-exchange reaction between ethylenedicysteine (EC) and ~(99m)Tc-glucoheptonate (~(99m)Tc-GH)

A detailed kinetic study of ligand-exchange reaction between 99mTc-GH and EC was carried out. The rate constants (k) of ligand-exchange reaction at different EC concentrations and different pH values were calculated. The k values (L · mol-1 · min-1) were 3.7 × 103, 4.1 × 103, 3.9 × 103 and 3.9 ×88 103 at EC concentrations (μmol · L-1) of 559.7, 279.9, 186.6 and 55.97, respectively, while k values were 3.7×103, 3.4×103, 1.9×103 and 3.1×88 102 when pH values were 10, 9, 8 and 7, respectively, and k had an increase of the order of magnitude from pH 7 to pH 8. It demonstrated that k of the ligand exchange reaction was pH dependent and pH value of the reaction solution must be equal to or little higher than 8 in order to make the labeling yield of 99mTc-EC higher than 90%.     

Study on the reaction kinetics of 99Tcm-labeled BIDP

A novel zoledronic acid derivative, 1-hydroxy-2-(2-butyl-1H-imidazole-1-yl)-ethylidene-1,1-diphosphonic acid(BIDP), was synthesized and labeled with <'99>Tc<'m>. The detailed kinetic study on the labeling reaction between BIDP and <'99>Tc<'m> was carried out. The results indicated that the reaction rote constants k were 0.0258, 0.0268,0.0305, 0.0323, 0.0351 and 0.0384 min<'-1> at 0℃, 5℃, 10℃, 15℃, 20℃ and 25℃, respectively. From the Arrhenius equation k=-A.e<'-Ea/(Rr)>, the activation energy Ea of the labeling reaction was calculated to be 10.45 kJ/mol. And the correlation between k and temperature(T) was also deduced as In k=1258.8×(1/7)+0.9531. In addition, it was found that in order to get a high radiolabeling yield(RLY)(>90%), the reaction temperature must be up to 12℃.     

Kinetic study on ligand-exchange reaction between ethylenedicysteine (EC) and 99mTc-glucoheptonate (99mTc-GH)

A detailed kinetic study of ligand-exchange reaction between 99mTc-GH and EC was carried out. The rate constants (k) of ligand-exchange reaction at different EC concentrations and different pH values were calculated. The k values (L@mol-1@min-1) were 3.7×103, 4.1×103, 3.9×103 and 3.9×103 at EC concentrations (?mol@L-1) of 559.7, 279.9, 186.6 and 55.97, respectively, while k values were 3.7×103, 3.4×103, 1.9×103 and 3.1×102 when pH values were 10, 9, 8 and 7, respectively, and k had an increase of the order of magnitude from pH 7 to pH 8. It demonstrated that k of the ligand exchange reaction was pH dependent and pH value of the reaction solution must be equal to or little higher than 8 in order to make the labeling yield of 99mTc-EC higher than 90%.     

^99mTc—MIBI心肌灌注显像潘生丁试验一天法在冠心病诊断中的应用

介绍了对１６１例冠心病患者采用＾９９ｍＴｃ－ＭＩＢＩ心肌显像潘生丁试验一天法方案进行检测的结果，其中２０例与冠状动脉造影进行了对比观察。患者先作＾９９ｍＴｃ－ＭＩＢＩ静态平面与断层心肌显像，接着进行潘生丁试验，再于静脉注射＾９９ｍＴｃ－ＭＩＢＩ后１ｈ作平面与断层心肌显像。采用本方法诊断冠心病的灵敏度为９１％。其检测ＬＡＤ的灵敏度为８８％；诊断ＲＣＡ及Ｌｃｘ的灵敏度分别为８１％与７７％、特异性分别为     

A comparative study of primary coronary stenting and intravenous thrombolysis for acute myocardial infarction using~(99m) Tc-MIBI SPECT imaging

1 INTRODUCTIONRe-perfusion therapy is one of the important progresses of acute myocardial infarction (AMI) therapy. It is established that early intravenous thrombolysis is effective and convenient for patency-related artery (IRA)[']. But obvious limitation existsfor thrombolysis application such as low IRA patency rate, recuxrent ischeAnc events,high re-infarction rate, hemorrhage complication and so many contraindications. Recentyearst primary coronary steming has been developed as …     

~(99m)Tc标记的肝胆显像剂吡哆色氨酸(~(99m)Tc-PHT)的研制

1975年R.J.Baker等已使用 ~(99m)Tc标记的吡哆醛氨基酸类作为肝胆显像剂,但其制备繁复且需高压程序。其后采用亚锡离子作还原剂,已能在常压下进行标记。1982年Kato-Azuma等将吡哆醛和氨基酸缩合形成的亚胺键(R_2C=N-R′)经加压加氢还原为稳定的胺键(R_2HC-NHR′)。这样形成的化合物更为稳定,易于制备药盒,并免除了药盒中需要大量吡哆醛和氨基酸的缺点及减少了与螯合剂竞争的潜在可能。我们参考有关文献在常温常压下成功地制备了吡哆色氨酸(PHT),化学名N-{[(3-羟基-5-羟甲基)-2-甲基-4-吡啶]甲基}-L-色氨酸。本文报道其合成、药盒制备、标记、动物实验和初步试用的结果。