Screening techniques and sources of resistance to root rots and wilts in cool season food legumes

Soilborne, fungal pathogens of cool season food legumes, including seed and seedling blights, rot rots, and wilts are described. Seed and seedling diseases are caused primarily by Pythium and Rhizoctonia spp. The most important fungi causing root rots include Aphanomyces euteiches, Fusarium solani, Pythium spp., Sclerotium rolfsii, and Macrophomina phaseolina. Wilt is caused primarily by various host-specific forms of Fusarium oxysporum. This paper discusses these diseases and screening procedures that emphasize standardization of inoculum levels, maintenance of virulent pathogen cultures, inoculum growth media, environmental conditions, and host plant age. Sources of resistance to these diseases are discussed.     

Screening techniques and sources of resistance to foliar diseases caused by fungi and bacteria in cool season food legumes

Screening techniques are an important component of the overall strategy of breeding for resistance to diseases in cool season food legumes. Suitable screening methods have been developed for several major foliar diseases of chickpea, pea, faba bean, and lentil, and sources of resistance have been identified. International cooperation plays an important role in promoting research and keeping collections of cultivated species and their wild relatives. New biotechnological approaches are promising for enhancing the practical use of genes for resistance.     

Screening techniques and sources of resistance to nematodes in cool season food legumes

Identification of sources of resistance in cool season legumes to cyst (Heterodera spp.), root-knot (Meloidogyne spp.), and stem nematode (Ditylenchus dipsaci) is generally based on number of cysts on roots, root-knot nematode induced gall index, and stem nematode reproduction in shoot tissue, respectively. Various levels of resistance to cyst nematodes have been detected in chickpea and pea. Resistance has also been identified in chickpea, faba bean, and pea to the root-knot nematodes. Broad based durable sources of resistance to plant parasitic nematodes are required. Basic research is needed to develop transgenic plants with resistance based on hatch stimulants, inhibitors, toxins, or repellents found in antagonistic rhizosphere microorganisms. Selection of genotypes that favor development of beneficial rhizosphere microorganisms or root endophytes that increase the plant resistance to nematode infection deserves attention.     

Screening techniques and sources of resistance to root diseases in cool season food legumes

Summary Soil-borne fungal diseases are among the most important factors, limiting the yield of grain legumes in many countries worldwide. Root rot, caused by Aphanomyces euteiches, Rhizoctonia solani, Fusarium solani and wilt, caused by several formae speciales of Fusarium oxysporum are the most destructive soil-borne diseases of pea, chickpea, lentil, fababean and lupin. The most effective control of these diseases is achieved through the use of resistant varieties. In this paper, recent advances in conventional and innovative screening methods for disease resistance are presented. Many grain legume accessions, which are maintained in national and international germplasm collections, have been evaluated for disease resistance and numerous resistant varieties have been released following incorporation of identified resistance genes from these sources. Recent identification of molecular markers tightly linked to resistance genes has greatly enhanced breeding programs by making marker assisted selection (MAS) possible and allowing the development of varieties with multiple disease resistance. Progress in the understanding of the biology of soil-borne fungal pathogens of grain legumes is also reviewed with particular reference to the genetic structure of their populations, diagnosis and host–pathogen interaction.     

Using host plant resistance to manage biotic stresses in cool season food legumes

Summary Kabuli chickpea (Cicer arietinum L.) is the common cultivated type of chickpea in arid and semi-arid environments in the Mediterranean region. Ascochyta blight, (Ascochyta rabiei (Pass.) Labr.), leaf miner (Liriomyza cicerina, Rond.) and cold, are the three most important stresses on chickpea grown under semi-arid conditions in this region. Phenotypic frequencies for responses to these stresses in the eight major chickpeagrowing regions of the world were estimated from 5,672 kabuli chickpea accessions assembled from these regions. In addition, the accessions were evaluated for 12 morpho-physiological and three phenological characters under semi-arid Mediterranean conditions at the International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria. Considerable regional differences in frequency distributions for response to the three stresses were observed. Average phenotypic diversity for responses to the three stresses was lower (H_o=0.474) than for morpho-physiological (H_o=0.754) and phenological (H_o=0.812) characters. The highest frequencies of accessions resistance to Ascochyta-blight and leaf-miner were found in South Asia and South Central Asia, respectively. A small number of chickpea breeding materials of ICARDA showed a moderate level of tolerance to cold. A group of four characters showing the strongest bivariate association with each of the three stresses was identified from the latter group. Then, a discrete multivariate log-linear analysis of the five-way frequency table was performed for each group. The simplest log-linear model for each group included both two- and three-factor association terms, but no independent factors. This suggested the potential for indirect selection for stress tolerance using one or more of these associated characters. The roles of these characters in ideotype breeding of kabuli chickpea for arid and semi-arid Mediterranean conditions deserves careful assessment.     

Breeding methods and selection indices for improved tolerance to biotic and abiotic stresses in cool season food legumes

The objective of breeding for stress tolerance is to improve productivity for a target level of stress. If tolerance is viewed as resistance to change in productivity with increasing stress, productivity under stress depends not only on stress tolerance, but also on maximum productivity. Index selection theory indicates that selection in non-stress environments will be more effective than direct selection for productivity under stress whenever the correlation between the two types of environments exceeds the heritability of productivity under stress. With high genetic correlation, selection should be conducted within a level of stress that maximizes heritability. In cases where heritability under non-stress is much higher than under stress, an index combining data from stress and non-stress environments is expected to be more efficient than selection based on evaluation only within stress environments.Secondary traits will be useful in breeding for productivity under stress whenever they have high heritability and high genetic correlation with productivity under stress. For some abiotic stresses and many biotic stresses, heritability will be highest in the presence of stress and indirect or index selection will be of limited value.     

Screening techniques and sources of tolerance to salinity and mineral nutrient imbalances in cool season food legumes

A large global land area is affected by saline, alkali (sodic), and acid soil conditions. Cool season food legumes are important crops in many countries with such adverse soils. Tolerant genotypes have been identified in many crops, including legumes. However, very little has been published on selection of tolerant cool season food legume crops. The inadequate knowledge and understanding of the responses of cool season food legume crops to these abiotic stresses, necessitates action by a collaborative network of interdisciplinary teams to make rapid progress in identifying tolerant germplasm and developing cultivars better adapted to unfavorable soil conditions.     

Water use and water use efficiency of cool season grain legumes in low rainfall Mediterranean-type environments

The water use (E_t) and water use efficiency (WUE) of a range of cool season grain legume species (field pea [Pisum sativum L.], faba bean [Vicia faba L.], chickpea [Cicer arietinum L.], lentil [Lens culinaris Med.], albus lupin [Lupinus albus L.], dwarf chickling [Lathyrus cicera L.], ochrus chickling [Lathyrus ochrus L.], grass pea [Lathyrus sativus L.], narbon bean [Vicia narbonensis L.], common vetch [Vicia sativa L.], and purple vetch [Vicia benghalensis L.]) were examined on fine textured neutral to alkaline soils in the low to medium rainfall Mediterranean-type environments in south-western Australia at Merredin and Mullewa in two seasons. There was no difference in the total E_t between grain legumes at either site in either year. There was also no variation in soil water extraction between species on the shallow sandy loam soil at Merredin. However, C. arietinum, L. sativus and L. cicera had greater water extraction and P. sativum the least water extraction at Mullewa where soil conditions were less hostile and root penetration was not restricted. The pattern of water use varied markedly between the grain legume species examined. Grain yield was positively correlated with post-flowering water use (E_tpa) in both erect (r=0.59) and prostrate (r=0.54) grain legume species. Water use efficiencies for dry matter production (WUE_dm) of up to 30 kg ha⁻¹ mm⁻¹ for V. faba and V. narbonensis at Merredin, and water use efficiencies for grain yield (WUE_gr) of up to 16 kg ha⁻¹ mm⁻¹ for P. sativum and 13 kg ha⁻¹ mm⁻¹ for V. faba at Mullewa, were comparable to those reported for cereals and other grain legumes in previous studies in this and other environments. Potential transpiration efficiencies (TE) of 15 kg ha⁻¹ mm⁻¹ together with soil evaporation (E_s) values of 100–125 mm were estimated in this and associated studies, and can be used as benchmark values to assess the yield potential of cool season grain legume crops in low rainfall Mediterranean-type environments. The major traits of adaptation for grain legume species producing large yields in this short season environment are early flowering, and pod and seed set before the onset of terminal drought. Early phenology together with rapid ground cover and dry matter production allows greater water use in the post flowering period. This leads to greater partitioning of dry matter into seed, which is reflected in greater harvest index (HI) and WUE_gr, as was observed for V. faba and P. sativum. Improvement in the adaptation of other grain legume species to short season Mediterranean-type environments requires increased early growth for rapid ground cover and improved tolerance to low temperatures (especially for C. arietinum) during flowering and podding.     

Analysis of host genotype x rhizobial strain interaction in N2 fixation

Summary A method of analyzing host genotype x rhizobial strain interaction for N₂ fixation potential, based on the principle of structural relationship analysis, is proposed. When this interaction is significant, selecting genotypes for high N₂ fixation potential is incorrect, because the N₂ fixation efficiency is dependent on the rhizobial strain used. Under such circumstances, grouping genotypes based on average fixing ability (AFA), a linear response to the effectiveness of rhizobial strain, and specific fixing ability (SFA), deviation from the linear response in terms of the magnitude of the error variance, is useful before initiating a breeding program for enhanced N₂ fixation. Host genotype x strain interaction effect is partitioned into two components, and two parameters are derived which estimate AFA and SFA. N₂ fixation data from 3 mungbean genotypes and 6 rhizobial strains are used to illustrate this method.     

Screening techniques and sources of resistance against parasitic weeds in grain legumes

Summary A number of parasitic plants have become weeds, posing severe constraints to major crops including grain legumes. Breeding for resistance is acknowledged as the major component of an integrated control strategy. However, resistance against most parasitic weeds is difficult to access, scarce, of complex nature and of low heritability, making breeding for resistance a difficult task. As an exception, resistance against Striga gesnerioides based on a single gene has been identified in cowpea and widely exploited in breeding. In other crops, only moderate to low levels of incomplete resistance of complex inheritance against Orobanche species has been identified. This has made selection more difficult and has slowed down the breeding process, but the quantitative resistance resulting from tedious selection procedures has resulted in the release of cultivars with useful levels of incomplete resistance. Resistance is a multicomponent event, being the result of a battery of escape factors or resistance mechanisms acting at different levels of the infection process. Understanding these will help to detect existing genetic diversity for mechanisms that hamper infection. The combination of different resistance mechanisms into a single cultivar will provide durable resistance in the field. This can be facilitated by the use of in vitro screening methods that allow highly heritable resistance components to be identified, together with adoption of marker-assisted selection techniques.     

Screening techniques and sources of resistance to rusts and mildews in grain legumes

Summary In this paper we review the existence of sources of resistance and the various available screening methods for resistance in grain legumes against the airborne pathogens powdery mildews, downy mildews and rusts. Available resistance against these pathogens is not abundant and is particularly in risk of erosion owing to the constant generation and introduction of new races of the pathogen. A continuous search for more resistance sources is therefore a priority in legume breeding and special emphasis should be paid to selection of resistance mechanisms that are likely to be durable and to implementation of strategies to prolong the durability of existing resistance.     

Heat tolerance in food legumes as evaluated by cell membrane thermostability and chlorophyll fluorescence techniques

Summary The genotypic variation for heat tolerance in chickpea, groundnut, pigeonpea, and soya bean was evaluated by testing membrane stability and photosystem (PSII) function in leaves at high temperatures. The legumes could be ranked from heat-tolerant to sensitive in the order: groundnut, soya bean, pigeonpea and chickpea. The damage to cell membranes (as reflected by an increased leakage of electrolytes) and PS II (as reflected by a decrease in the ratio of variable to maximum fluorescence) was less, and recovery from heat stress was faster in groundnut than in other crops. Prior exposure of plants to 35°C for 24h led to a reduced leakage of electrolytes at high temperatures in all crops but the differences among legumes were consistent. Substantial genotypic variation for heat tolerance was found in all legumes. Membrane injury was negatively associated with specific leaf weight in groundnut (r=–0.69^**) and soya bean (r=–0.56^**) but not in the pulses. Electrolyte leakage and fluorescence ratio were negatively correlated in all legumes. The potential use of electrolyte leakage and fluorescence tests as screening procedures for breeding heat-tolerant legumes is discussed.Abbreviations RI relative injury - Fo initial fluorescence - Fm maximum fluorescence - Fv variable fluorescence - PS II photosystem II - PAR photosynthetically active radiation     

Effects of soybean variety and Bradyrhizobium strains on yield,protein content and biological nitrogen fixation under cool growing conditions in Germany

Soybean (Glycine max (L.) Merr.) is able to fix atmospheric nitrogen in symbiosis with the bacteria Bradyrhizobium japonicum. Because these bacteria are not native in European soils, soybean seeds must be inoculated with Bradyrhizobium strains before sowing to fix nitrogen and meet their yield potential. In Central Europe soybean cultivation is still quite new and breeding of early maturing soybean varieties adapted to cool growing conditions has just started.Under these low temperature conditions in Central Europe the inoculation with different, commercially available Bradyrhizobium inoculants has resulted in unsatisfactory nodulation. The aim of this study was: (i) to test the ability of commercially available inoculants to maximize soybean grain yield, protein content and protein yield, (ii) to study the interaction of different inoculants with different soybean varieties for two different sites in Germany under cool growing conditions over three years and (iii) to determine the variability of biological nitrogen fixation. Field trials were set up on an organically managed site at the Hessische Staatsdomäne Frankenhausen (DFH) and on a conventionally managed site in Quedlinburg (QLB) for three consecutive seasons from 2011 to 2013. Three early maturing soybean varieties—Merlin, Bohemians, Protina—were tested in combination with four different Bradyrhizobium inoculants—Radicin No.7, NPPL-Hi Stick, Force 48, Biodoz Rhizofilm—and compared with a non-inoculated control. Effective inoculation with Bradyrhizobium strains increased grain yield, protein content and protein yield by up to 57%, 26% and 99%, respectively. Grain yield, protein content and protein yield were generally higher in DFH. Average grain yield was 1634 kg ha⁻¹ in QLB (2012–2013) and 2455 kg ha⁻¹ in DFH (2011–2013), average protein content was 386 g kg⁻¹ in QLB and 389 g kg⁻¹ in DFH and average protein yield was 650 kg ha⁻¹ in QLB and 965 kg ha⁻¹ in DFH. The percentage of nitrogen derived from air (Ndfa) ranged between 40% and 57%. Soybeans inoculated with Radicin No. 7 failed to form nodules, and crop performance was identical to the non-inoculated control. Biodoz Rhizofilm, NPPL Hi-Stick and Force 48 are suitable for soybean cultivation under cool growing conditions in Germany. Interactions between soybean variety and inoculant were significant for protein content and protein yield at both sites, but not for nodulation, grain yield, thousand kernel weight and Ndfa. The variety Protina in combination with the inoculant Biodoz Rhizofilm can be recommended for tofu for both tested sites, while Merlin and Protina in combination with Biodoz Rhizofilm are recommended for animal fodder production in DFH. Animal fodder production was not profitable in QLB due to low protein yields.     

Screening techniques and sources of resistance to foliar diseases caused by major necrotrophic fungi in grain legumes

Summary Necrotrophic pathogens of the cool season food legumes (pea, lentil, chickpea, faba bean and lupin) cause wide spread disease and severe crop losses throughout the world. Environmental conditions play an important role in the development and spread of these diseases. Form of inoculum, inoculum concentration and physiological plant growth stage all affect the degree of infection and the amount of crop loss. Measures to control these diseases have relied on identification of resistant germplasm and development of resistant varieties through screening in the field and in controlled environments. Procedures for screening and scoring germplasm and breeding lines for resistance have lacked uniformity among the various programs worldwide. However, this review highlights the most consistent screening and scoring procedures that are simple to use and provide reliable results. Sources of resistance to the major necrotrophic fungi are summarized for each of the cool season food legumes. Marker-assisted selection is underway for Ascochyta blight of pea, lentil and chickpea, and Phomopsis blight of lupin. Other measures such as fungicidal control and cultural control are also reviewed. The emerging genomic information on the model legume, Medicago truncatula, which has various degrees of genetic synteny with the cool season food legumes, has promise for identification of closely linked markers for resistance genes and possibly for eventual map-based cloning of resistance genes. Durable resistance to the necrotrophic pathogens is a common goal of cool season food legume breeders.     

Chlorophyll fluorescence as a tool in soybean improvement for N2 fixation efficiency

Summary Chlorophyll fluorescence, and more particularly the fluorescence decrease ratio (Rfd) which indicates the potential photosynthetic activity of the leaf is highly correlated with the plant nitrogen fixation activity, at stage R₂ (onset of flowering), under controlled growth conditions, using nutrient solutions where mineral nitrogen supply is decreasing. The Rfd value affords the analysis of the efficiency of the nitrogen fixation related to the host plant and the rhizobial strain, and the characterization of the best strain-genotype pairs. The rapidity of the measurement together with the availability of portable fluorescence determination systems make possible an application to soybean improvement for increased nitrogen fixation.     

Heterosis of interspecific soybean hybrids for traits related to N2 fixation

Summary Three greenhouse experiments were conducted to compare the performance of soybean (Glycine max (L.) Merr.), wild soybean (G. soja Sieb. et Zucc.), and soybean x wild soybean hybrids for traits relating to N₂ fixation including nodulation, acetylene reduction, nodule leghemoglobin concentration, and nitrogen (N) accumulation and dry matter (DM) accumulation. In all three experiments G. max generally exceeded G. soja in nodulation, acetylene reduction, and N and DM accumulation while the soybean possessed higher nodule leghemoglobin concentration. In Experiment I, the mean of the hybrids did not differ significantly from the G. max parent in nodule mass, acetylene reduction activity, nodule leghemoglobin concentration, or DM accumulation. The hybrids did exceed the soybean parent in N accumulation, thus demonstrating high parent heterosis. In Experiments IIA and IIB with a more carefully chosen set of G. soja parents, high parent heterosis of individual crosses was common. Across the three experiments average high parent heterosis was 34, 28, and 28%, respectively, for nodule mass, N accumulation, and DM accumulation. If one accepts the assumption that hybrid vigor results from the accumulation of dominant alleles, then these alleles could theoretically be accumulated via selection in a homozygous genotype. If this is true than the results of the experiments reported here suggest that interspecific soybean x wild soybean crosses could serve as sources of homozygous lines which would exceed currently available soybean cultivars in nodule mass, and N and DM accumulation.This work was supported in part by the USDA Competitive Grants Program, Grant 82-CRCR-1-1039 and Cooperative Agreement 58-32U4-2-370 between the U.S. Department of Agriculture and the Agronomy Department, University of Maryland, Scientific Article No. A-4648, Contribution No. 7644 of the Maryland Agricultural Experiment Station.     

Effects of walnut trees on biological nitrogen fixation and yield of intercropped alfalfa in a Mediterranean agroforestry system

While intercropping annual non nitrogen-fixing crops with deciduous hardwood species is now well documented, there is a need to investigate if nitrogen-fixing intercrops may succeed in agroforestry systems. Intercropping with trees usually leads to a decline in crop yield, and could in addition possibly reduce the biological N fixation (BNF) over time due to the competition for resources. In a Mediterranean experimental site, 17 year-old hybrid walnut trees (Juglans nigra x Juglans regia L.) planted in East-West oriented lines were intercropped with alfalfa (Medicago sativa L.) to assess the impact of competition for light and water on alfalfa yield and BNF. Alfalfa yield and shoot δ¹⁵N values (a proxy for the proportion of N derived from the air, %Ndfa) were measured during one year at different distances from the tree row in two directions (north and south). Alfalfa yield was reduced close to the tree row (−28% and −22% on the northern and southern sides respectively), but less than the reduction of irradiation (−59% and −33% respectively). Shading improved by 35% the apparent light use efficiency (LUE: aboveground biomass produced per unit of global radiation) of alfalfa, indicating that alfalfa was shade tolerant at this Mediterranean site. Alfalfa shoot δ¹⁵N values were lower close to the tree rows than at mid inter-row: BNF was stimulated close to the trees. Compensative and facilitative mechanisms between trees and alfalfa plants led to a rise in LUE and%Ndfa in shaded areas. These results contradict the frequent assumption that N fixation is reduced in the shade of trees. Appropriate tree canopy management may help maintain light competition between trees and alfalfa to a level that still enhance complementary, which would further improve the sustainability of the use of alfalfa as an intercrop in Mediterranean regions.     

Cytoplasmic male sterility,resistance to gall mite and mildew,and season of leafing out in black currants

Summary Cytoplasmic male sterility (cms) is described in the F₁ hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf ₁) and recessive for the other (Rf ₂).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph ₃(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf ₁(one of two dominant additive genes controlling early season leafing out) indicated that Rf ₁and Rf ₂were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf ₁, and 0.15 for Lf ₁-Sph ₃were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph ₃.It is suggested that competitive disadvantage of lf ₁-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph ₃. Poor performance of lf ₁- (and possibly lf ₂-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off).     

尿素及秸秆添加对华中地区茶园土壤CO_2和N_2O排放的影响

为了明确尿素和秸秆添加对茶园土壤CO_2和N2O排放的影响,为茶园合理施肥提供理论依据,本研究在室内培养条件下,以华中地区红壤丘陵区茶园土壤为对象,运用静态培养系统研究方法,研究该土壤在尿素输入和作物秸秆添加后CO_2和N_2O的排放特征。培养试验共设置对照、尿素、作物秸秆和尿素+作物秸秆4种处理。结果表明:不同处理下华中地区茶园土壤CO_2和N_2O排放呈显著差异。作物秸秆添加显著提高茶园土壤CO_2的排放,作物秸秆和作物秸秆+尿素处理分别是对照的5.57和4.99倍。尿素输入显著促进茶园土壤N_2O的排放,而秸秆添加却降低N_2O的排放。对照和添加尿素处理土壤N_2O排放通量与铵态氮含量呈显著正相关关系。无秸秆添加处理土壤N_2O排放通量与硝态氮含量呈显著的相关性,而添加秸秆处理二者无显著相关关系。土壤可溶性有机碳含量对CO_2排放有显著影响,二者之间呈极显著线性正相关关系。