西帕依固龈液对内毒素诱导人牙龈成纤维细胞分泌IL-6的抑制作用

目的：研究不同浓度西帕依固龈液对内毒素（lipopolysaccharide，LPS）介导的人牙龈成纤维细胞（human gingival fibroblast，HGF）分泌白介素-6（IL-6）水平的影响。方法：采用健康人正常牙龈组织原代培养的HGF，以25mg／L的LPS作为刺激因子，用放射免疫法测定细胞培养上清液中IL-6的水平，以观察5种浓度（12．5，25，50，100，200mg／L）西帕依固龈液对HGF分泌炎性细胞因子的影响。结果：西帕依固龈液可显著抑制LPS诱导HGF分泌IL-6水平，其作用在一定范围内呈浓度依赖性。结论：西帕依固龈液能够抑制LPS诱导HGF分泌IL-6的水平，提示固龈液具有一定的抗炎作用，有助于对牙周病的防治。     

西帕依固龈液对牙龈卟啉单胞菌体外抑菌作用的研究

目的 比较西帕依固龈液、氢氧化钙和次氯酸钠在体外对牙龈卟啉单胞菌的抑菌杀菌作用,为临床推广使用西帕依固龈液提供实验依据.方法 ①采用液体稀释法药敏实验检测西帕依固龈液对牙龈卟啉单胞菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC).②采用液体扩散法比较观察西帕依固龈液、氢氧化钙和次氯酸钠对牙龈卟啉单胞菌抑菌圈大小.结果 ①西帕依固龈液对牙龈卟啉单胞菌的MIC和MBC分别为5mg/mL和10 mg/mL.②比较5mg/mL和10 mg/mL西帕依固龈液与5mg/mL次氯酸钠对牙龈卟啉单胞菌的抑菌作用,差异有统计学意义(P＜0 05).5 mg/mL西帕依固龈液与3mg/mL氢氧化钙、20 mg/nL的西帕依固龈液与5 mg/mL次氯酸钠对牙龈卟啉单胞菌的抑菌作用,差异无统计学意义(P＞0.0)5).结论 在体外抑菌实验中,选择合适的浓度,西帕依固龈液对牙龈卟啉单胞菌的抑菌作用可以与氢氧化钙和次氯酸钠相当.     

维药西帕依固龈液对实验性牙龈炎模型大鼠牙龈组织中NF-κB p65、COX-2表达的影响

目的:探讨牙龈炎的发病机制及西帕依固龈液治疗牙龈炎的作用机制。方法:36只Wistar大鼠随机分为正常组、牙龈炎模型组、西帕依固龈液组,后两组采用右侧上颌第二磨牙牙颈部丝线结扎、高糖喂饲加牙龈卟啉单胞菌接种的方法制备大鼠牙龈炎实验模型,西帕依固龈液组大鼠患牙局部用西帕依固龈液冲洗,牙龈炎模型组大鼠患牙局部每天用生理盐水冲洗1周。第3周将每组大鼠处死,取右上颌第二磨牙的牙龈组织,镜下观察病理组织学改变,并采用半定量逆转录聚合酶链反应(RT-PCR)的方法检测各组牙龈组织中核因子κBp65(NF-κBp65)、环氧化酶-2(COX-2)信使核糖核酸(mRNA)的表达,并分析模型组中NF-κBp65与COX-2表达的相关性。结果:牙龈炎模型组大鼠牙龈上皮增生,炎性细胞浸润增多;西帕依固龈液组炎性细胞减少,组织修复愈合。牙龈炎模型组大鼠牙龈组织中COX-2表达比正常组明显升高(P<0.05),NF-κBp65表达比正常组升高,但差异无统计学意义(P>0.05)。西帕依固龈液组大鼠牙龈组织中NF-κBp65和COX-2表达明显低于牙龈炎模型组(P<0.01)。牙龈炎模型组NF-κBp65与COX-2的表达呈正相关(r=0.699,P<0.05)。结论:在大鼠实验性牙龈炎牙龈组织中,NF-κBp65的激活可能调控COX-2的表达升高,而西帕依固龈液能抑制牙龈炎牙龈组织中NF-κBp65和COX-2的表达。     

西帕依固龈液治疗牙龈炎和控制菌斑的临床疗效观察

目的：观察和评价西帕依固龈液治疗牙龈炎及控制菌斑的临床疗效。方法：53例单纯性牙龈炎患者随机分为2组，实验组28例用西帕依固龈液含漱，对照组25例用0．9％生理盐水含漱，每天5次，1周后复诊。观察治疗前后临床症状和菌斑指数、牙龈指数、龈沟出血指数的变化，并采集龈下菌斑，进行刚果红染色，镜检计数螺旋体、球菌等菌的变化。结果：2组患者治疗前牙周各项指数的平均值差异无统计学意义（P〉0．05）。治疗后实验组的牙龈指数、龈沟出血指数及菌斑指数均有明显改善（P〈0．01），镜检螺旋体计数减少，球菌、杆菌数量明显增加，与用药前比较差异有统计学意义（P〈0．01）。结论：西帕依固龈液作为一种治疗牙龈炎的口腔用药，可明显改善菌斑的组成，促进正常口腔生态环境的建立。     

中药含漱液用于维护固定矫治患者牙周健康的临床观察

目的观察西帕依固龈液用于维护固定矫治患者牙周健康的效果。方法选择44例固定矫治患者，随机分为西帕依固龈液组和口泰含漱液组，于洁治及用药前、洁治及用药后1周、1个月、3个月分别检查牙龈指数（a）、牙龈出血指数（SBt）、菌斑指数（PLI）及牙冠染色指数（SI）。结果用药后3个月西帕依固龈液组的GI、SBI、PLI均比用药前有明显降低（P〈0．001），而与口泰漱口液组相比则没有明显差异；但是西帕依固龈液组的SI则显著低于口泰漱口液组。结论西帕依固龈液能有效抑制菌斑形成，维护固定矫治患者牙周健康，长期使用没有明显副作用。     

西帕依固龈液治疗妊娠期牙龈出血临床疗效

目的：评价西帕依固龈液治疗妊娠期牙龈出血的临床疗效。方法：将60例妊娠期牙龈出血患者随机分成两组,观察组使用西帕依固龈液含漱,对照组使用1％双氧水溶液含漱,10天后复查,对两者的治疗效果进行比较。结果：西帕依固龈液对治疗妊娠期牙龈出血总有效率90％．1％双氧水溶液治疗妊娠期牙龈出血有效率76．6％,两组疗效差异有显著性（P〈0．05）。结论：西帕依固龈液对治疗妊娠期牙龈出血有很好的临床疗效。     

西帕依固龈液体外抑制致臭菌及防治口臭的效果评价

目的:本研究通过体外抑菌和体内试验,评价西帕依固龈液对口腔主要致臭菌抑菌作用及体内防治半胱氨酸激发性口臭的效果,为其进一步在临床卷烟厂用提供依据.方法:采用琼脂稀释法,测定西帕依固龈液对牙龈卟啉单胞菌(Pg)、中间普雷沃菌(Pi)、具核梭杆菌(Fn)的最低抑菌浓度(MIC);对10例志愿者采用自身前后对照试验,观察四种干预措施(无干预、清水、西帕依固龈液、口泰)对半胱氨酸激发性口臭的最大值水平、各时间点下降百分比及口气值的下降曲线的影响,评价西帕依围龈渡防治口臭的效果.结粟:西帕依固龈液对Pg、Fn的MIC是的1mg/mL,对Pi的MIC是0.5mg/mL;西帕依同龈液对半胱氨酸激发的口臭试验亦有显著的防治效果.结论:西帕依固龈液对口腔主要致臭菌具有明显的抑制作用,可以有效抑制半胱氨酸激发的口臭.     

西帕依固龈液在胃肠减压患者口腔护理中的应用

目的:观察西帕依固龈液用于胃肠减压患者口腔护理的作用.方法:特患者分成实验组、对照组两组,实验组应用西帕依固龈液纱布作口腔护理,对照组应用0.9％盐水纱布做口腔护理.结果:实验组在牙龈红肿、出血、口臭的患病率明显低于对照组(P＜0.01).结论:西帕依固龈液在防治胃肠减压患者并发牙龈红肿、出血、口臭等方面疗效明显,不良反应小.     

西帕依固龈液用于感染单根管消毒的疗效观察

目的观察西帕依固龈液消毒感染单根管的临床疗效。方法选择感染单根管患牙79颗，常规根管预备后，随机分为西帕依固龈液组（41颗）和甲醛甲酚组（38颗），分别观察封药后临床症状和体征的变化。结果两组封药后，西帕依固龈液组患者疼痛不适反应明显低于甲醛甲酚组（P〈0．05）。结论西帕依固龈液在单根管封药中，是一种较安全、有效的根管消毒药物。     

西帕依固龈液预防固定正畸患者早期牙龈炎的临床疗效观察

目的观察西帕依固龈液预防固定正畸患者早期牙龈炎的临床疗效。方法选取固定正畸患者40例,随机分成试验组（使用西帕依固龈液和常规口腔卫生保健措施）和对照组（常规口腔卫生保健措施）,每组20例,正畸治疗开始后试验组使用西帕依固龈液含漱。观察患者正畸治疗前和治疗后1、2、3个月牙龈炎的发生情况,分别测定各指数牙的牙龈指数（GI）、菌斑指数（PLI）以及龈沟出血指数（SBI）,并进行统计学分析。结果固定正畸治疗前,试验组和对照组患者牙龈状况良好,牙周各临床指标值GI、PLI、SBI相比差异均无统计学意义（P〉0.05）;固定正畸治疗1个月及2个月后复查,试验组和对照组牙周各临床指标值均较治疗前上升,但对照组上升更明显,与试验组相比均有统计学意义（P〈0.05）,并且治疗2个月后试验组SBI值与对照组相比差异有高度统计学意义（P〈0.01）;固定正畸治疗3个月后复查,试验组和对照组牙周各临床指标值继续上升,两组相比差异均有高度统计学意义（P〈0.01）。结论西帕依固龈液能有效减少固定正畸患者早期牙龈炎的发生。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.