COX-2和VEGF的表达与胃癌生物学行为的关系

目的 :探讨 COX- 2和 VEGF的表达与胃癌临床病理特征之间的关系以及 COX- 2与 VEGF表达的相关性。方法 :收集徐州医学院附属医院临床资料完整的手术切除胃癌标本 84例、胃镜活检正常胃黏膜标本 2 0例。采用免疫组化 Power vision TM两步法检测 COX- 2、VEGF的表达。结果 :1正常胃黏膜组织中 COX- 2和 VEGF均呈阴性。胃癌组织中 COX- 2及 VEGF的阳性表达率分别为 6 6 .6 7% (5 6 / 84 )和 5 9.5 2 % (5 0 / 84 ) ,均明显高于正常胃黏膜组织(P<0 .0 1) ;2胃癌标本中 COX- 2的表达与癌灶大小、淋巴结转移、癌浸润深度和病理类型显著相关 (P<0 .0 5或 0 .0 1) ;而 VEGF表达与胃癌临床病理特征之间无关 (P>0 .0 5 )。 3VEGF表达率在 COX- 2阳性胃癌中为 75 .0 0 % (42 /5 6 ) ,COX- 2阴性者 35 .71% (10 / 2 8) ,前者明显高于后者。 VEGF与 COX- 2表达呈显著正相关 (P<0 .0 5 )。结论 :COX- 2的表达与胃癌的发生、发展密切相关 ,COX- 2的检测有助于评价胃癌的生物学行为、判断胃癌的预后。 COX- 2表达与 VEGF显著相关 ,推测 COX- 2可能通过诱导 VEGF表达上调参与胃癌血管生成。     

COX-2和VEGF在胃癌组织中的表达及意义

目的探讨环氧化酶-2(cyclooxygenagse-2,COX-2)、血管内皮生长因子(vascul( )r endothelial cell growth factor,VEGF)在胃癌中的表达及意义.方法用免疫组化S-P法检测45例胃癌、癌旁组织COX-2、VEGF的表达.结果胃癌组织COX-2、VEGF的阳性表达率分别是77.78%和75.56%,明显高于癌旁组织(P＜0.01,P＜0.05);COX-2、VEGF均与胃癌的浸润浓度、淋巴结转移和临床分期密切相关(p＜0.05):COX-2( )组VEGF表达率显著高于COX-2(-)组(p＜0.05).结论COX-2.VEGF与胃癌的浸润转移及病期进展有关,COX-2可上调VEGF表达.     

COX-2和P53基因在食管癌组织中的表达及其意义

目的研究环氧合酶-2(COX-2)、P53基因在食管癌组织中的表达及其意义.方法采用免疫组化染色法检测COX-2、p53在82例食管鳞状细胞癌组织中的表达,并与16例正常食管粘膜组织比较.结果COX-2和P53在食管癌组织中的阳性表达率分别为87.8%(72/82)和82.9%(68/82),而正常食管组织中均为阴性.COX-2在高分化和中分化食管癌中的表达率显著高于低分化癌(P＜0.01);无淋巴结转移者的表达率明显高于有淋巴结转移病例(P＜0.05).p3高表达与食管癌浸润深度和淋巴结转移呈正相关(P＜0.01).结论 COX-2和P53的表达可能均参与了食管癌的发生 发展过程.     

COX-2表达和微血管生成与胃癌的关系

目的探讨环氧化酶-2(COX-2)在胃癌组织中的表达与微血管生成的相关性及其与胃癌生物学行为之间的关系,从而为临床治疗和判断预后提供新的依据。方法应用免疫组化SP法对90例胃癌患者胃癌及癌旁组织中COX-2蛋白表达和微血管密度(MVD)进行检测,分析其与胃癌组织分化程度、浸润深度、淋巴结转移和预后的关系。结果胃癌组织COX-2的阳性表达率(667%)明显高于癌旁肠化及非典型增生COX-2阳性表达率(356%),P<001。COX-2阳性者MVD值(4866±1789)显著高于阴性者MVD值(2414±1217),P<001。COX-2的阳性表达与胃癌组织分化程度无关,而与胃癌浸润深度、淋巴结转移密切相关(P<005);胃癌组织MVD值(4049±1988)明显高于癌旁肠上皮化生及非典型增生MVD值(1981±1133);MVD与胃癌组织分化程度、浸润深度、淋巴结转移密切相关(P<005)。结论COX-2可促进肿瘤血管形成,从而进一步促进肿瘤的浸润、转移。COX-2、MVD均可作为反映胃癌生物学行为的指标之一。     

胃癌组织中COX-2、VEGF和MVD的表达及临床意义

目的 观察环氧合酶-2(COX-2)、血管内皮生长因子(VEGF)和微血管密度(MVD)在正常胃黏膜组织、癌旁组织和胃癌组织中的表达,探讨COX-2的表达与肿瘤血管生成的关系?方法 采用SP免疫组化方法检测30例正常胃黏膜组织、50例癌旁组织和50例胃癌组织中COX-2、VEGF和MVD的表达?结果 正常胃黏膜组织→癌旁组织→胃癌组织中,COX-2的表达强度呈逐渐增加(P<0.01),COX-2的表达与肿瘤的分化程度和淋巴结转移有关(P<0.01),而VEGF的表达和MVD呈逐渐上升趋势,两者表达与肿瘤的分化程度、浸润深度和淋巴结转移有关?COX-2与MVD的表达呈正相关。结论 COX-2的异常表达及其诱导的血管生成与胃癌的分化程度、淋巴结转移密切相关。     

环氧合酶-2蛋白在胃癌发生发展中的表达

目的研究环氧合酶-2（COX-2）蛋白在胃癌、癌前病变、淋巴结转移灶等组织中的表达。方法应用免疫组化SP法和RT—PCR法检测甘肃省河西地区90例胃癌,及其80例增生性病变,22例正常胃黏膜上皮和29例淋巴结转移灶中COX-2的表达。结果免疫组化结果提示正常胃黏膜上皮、增生病变及原位癌中均未发现有COX-2的表达;无淋巴结转移的胃癌原发灶中COX-2的阳性表达率是46％,有淋巴结转移的胃癌原发灶中阳性表达率是72％,淋巴结转移灶中阳性表达率是90％。在鳞状细胞浸润癌中COX-2呈低表达（14％）,在浸润性腺癌中高表达（78％）。RT-PCR结果与免疫组化结果相似。结论COX-2的表达与胃癌的浸润、淋巴结转移有关,其表达具有组织选择性。     

多药耐药基因产物及p53、Bcl-2蛋白在胃癌组织中的表达

目的 探讨多药耐药基因产物P -糖蛋白(P - gp)、谷胱甘肽 -S-转移酶π(GSTπ)及DNA拓扑异构酶Ⅱ(TopoⅡ)、p53、Bcl-2蛋白在胃癌发生、发展和耐药性产生中的作用以及相互间的关系。 方法 应用免疫组化SP法检测胃癌组织中P - gp、GSTπ及TopoⅡ与 p53、Bcl-2蛋白的表达情况。结果 胃癌组织中P - gp在淋巴结转移组和癌组织浸润达浆膜外组的阳性表达率显著高于无淋巴结转移组和浆膜内浸润组的阳性表达率(P<0.05) ;GSTπ及p53蛋白在淋巴结转移组的阳性表达率显著高于无淋巴结转移组的阳性表达率(P<0.05) ;Bcl-2蛋白的阳性表达率为50.0% ,高分化腺癌的阳性表达率显著高于未分化癌的阳性表达率(P<0.05)。胃癌组织中P- gp与 p53、Bcl-2蛋白的表达有正相关性 (r>0.3)。 结论 p53、Bcl-2蛋白参与了胃癌的发生 ;P - gp阳性表达提示胃癌细胞对化疗药物存在原发性耐药 ,同时与癌细胞浸润深度、淋巴结转移有关。有多种耐药机制参与了胃癌耐药性的发生。     

中性粒细胞淋巴细胞比、环氧合酶-2及核转录因子-κB在食管鳞状细胞癌中表达的意义

目的探讨中性粒细胞淋巴细胞比(NLR)、环氧化酶-2(COX-2)、核转录因子-κB(NF-κB)在食管鳞状细胞癌中的表达及相互之间的关系。方法选取新乡医学院第一附属医院经病理诊断为食管鳞状细胞癌的患者60例(鳞癌组),另选择同期健康体检者42例(对照组),分别检测2组受试者外周血常规并计算NLR,比较2组受试者NLR的差异,并进一步分析NLR与食管鳞状细胞癌病理特点的关系。采用免疫组织化学方法检测食管鳞状细胞癌患者癌组织及癌旁组织中COX-2、NF-κB的表达。鳞癌组患者以NLR中位值3.0为节点分为低NLR组(NLR<3.0)和高NLR组(NLR≥3.0),分析2组患者癌组织中COX-2、NF-κB表达的差异。采用Spearman等级相关分析COX-2与NF-κB表达的相关性。结果鳞癌组和对照组受试者NLR分别为1.79±0.77、3.75±2.22,鳞癌组受试者NLR显著高于对照组(P<0.05)。NLR与患者的性别、年龄、肿瘤大小、肿瘤位置无明显相关性(P>0.05),与浸润深度、淋巴结转移、远处转移、病理分级、临床分期有相关性(P<0.05)。COX-2和NF-κB在食管鳞状细胞癌组织中的阳性表达率分别为81.7%(49/60)和56.7%(34/60),在癌旁组织中的阳性表达率分别为58.9%(35/60)和36.7%(22/60),COX-2和NF-κB在食管鳞状细胞癌组织中的阳性表达率均显著高于癌旁组织(P<0.05)。COX-2表达与肿瘤大小及浸润深度相关(P<0.05),与年龄、肿瘤部位、淋巴结转移、远处转移及病理分级无关(P>0.05)。NF-κB表达与肿瘤浸润深度、淋巴结转移、远处转移有关(P<0.05),与年龄、肿瘤大小、肿瘤部位、病理分级无关(P>0.05)。COX-2和NF-κB在食管鳞状细胞癌组织中表达呈正相关(P<0.05)。高NLR组患者癌组织中COX-2和NF-κB阳性表达率分别为94.1%(32/34)和73.5%(25/34),显著高于低NLR组的65.4%(17/26)和34.6%(9/26)(P<0.05)。结论NLR可能是评价食管鳞状细胞癌恶性生物学行为的指标;COX-2、NF-κB可能通过促进慢性炎症的过程参与食管鳞状细胞癌的发生、发展。     

浅析MMP-7与COX-2的表达与胃癌组织生物学行为的关系

目的 探讨癌组织中环氧合酶(COX-2)和基质金属蛋白酶(MMP-7)的表达与胃癌组织生物学行为的关系.方法 选取55例进展期胃癌手术的切除标本,以免疫组织化学SP法检测病灶中COX-2和MMP-7的表达情况.分析COX-2和MMP-7的表达与临床病理因素的关系.结果COX-2和MMP-7的阳性表达率分别为63.6%和72.7%.COX-2的阳性表达率与肿瘤大体类型、分化程度、浸润深度、淋巴结转移、TNM分期先关(P＜0.05);MMP-7的阳性表达率与浸润深度、淋巴结转移、TNM分期先关(P＜0.001),而与肿瘤大体类型和分化程度无关(P＞0.05),胃癌组织中COX-2和MMP-7的表达呈等级正相关(P＜0.001).结论 COX-2和MMP-7与胃癌组织生物学行为密切相关,其高表达是胃癌组织高侵袭能力和预后不良的标志之一.COX-2和MMP-7可作为反应胃癌组织侵袭转移及判断预后的生物学指标.     

胃癌原发灶中p~(53)和ki-67的表达及其意义

目的探讨胃癌原发病灶中p53及ki-67基因蛋白表达与胃癌的分化、浸润程度及淋巴结转移的关系。方法采用免疫组化法检测70例胃癌原发肿瘤组织中p53及ki-67基因蛋白的表达。结果胃癌原发肿瘤组织中p53、ki-67主要表达在细胞核,阳性表达率分别为72.9%(51/70)和31.4%(22/70),p53的阳性表达率显著高于ki-67的阳性表达率(P<0.01);p53基因蛋白的表达与淋巴结转移显著相关(P<0.05);而ki-67基因蛋白表达则与肿瘤的浸润深度呈显著相关(P<0.05)。结论胃癌组织中ki-67的表达与胃癌浸润有关,检测突变型p53对判断胃癌淋巴结转移具有重要的参考价值。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.