RELATION OF MALIGNANT TUMOR TO PROSTACYCLIN AND THROMBOXANE

ＲＥＬＡＴＩＯＮＯＦＭＡＬＩＧＮＡＮＴＴＵＭＯＲＴＯＰＲＯＳＴＡＣＹＣＬＩＮＡＮＤＴＨＲＯＭＢＯＸＡＮＥ￥ＴａｎＹａｙｉｎｇ；谈亚英；ＷａｎｇＢｉｎ；王彬；ＬｉｎＦｕｇｕａｎｇ；刘富光；ＺｈｏｕＬｉａｎｍｅｉ；周连妹；ＬｉｎＪｕｎ；刘俊（Ｄｅｐａｒｔ...     

EFFECTS OF PHYTOLACCA ACINOSA POLYSACCHARIDES I ON CYTOTOXICITY OF MACROPHAGES AND ITS PRODUCTION OF TUMOR NECROSIS FACTOR AND INTERLEUKIN 1

The in vivo effects of Phytolacca acinosa poly-saccharides I (PEP-I) on immunologic cytotoxicity of mouse peritoneal macrophages and its production of tumor necrosis factor (TNF) and interleukin 1 (IL-1) were studied. PEP-I 80 or 160 mg kg was given ip twice every 4 day. Both doses were found to have significant enhancing activity on macrophages cytotoxicity against S180 sarcoma cells and malignant transformed fibroblast L929 cells. Peritoneal activated macrophages were incubated with LPS for 2 and 24 hrs to induce TNF and IL-1, respectively. The TNF and IL-1 activities were tested from cytotoxicity against L929 cells in an absorbence assay of enzymatic reaction and proliferation of thymocytes co-stimulated assay separately. The optimal time for TNF production was found on day 8. Significant increases in TNF and IL-1 were observed. In comparison of the effect of PEP-I on TNF with that of known priming agent BCG, there was no difference between them, but PEP-I had a high effect on IL-1. These results sug     

SYNTHESIS AND EXPRESSION OF A GENE FOR HUMAN TUMOR NECROSIS FACTOR-ALPHA (TNF-α)

TNF-α was found originally In sera of Bacillus Calmette Guerln infected mice as a macrophage derived factor. It Is cytotoxlc for tumor cell and less or not toxic to normal cells in vitor. The gene for human TNF-α with E. coli-preferred codons has been designed according to the amino acid sequence deduced from the cDNA. The gene with 504 bp was divided into 27 oligonucleotide fragments having 30. to 40 nucleotides each. The solid phase phosphotriester method was used for the synthesis of these oligonucleotides. The 27 fragments were annealed to three segments and then linked by T4 DNA ligase. The entire gene was incorporated into plasmld PDR540 with Tac promoter which was used to transform E. coli 7118. The expressed protein was estimated by SDSPAGE with a molecular weight of 1. 7×104Da. The cytotoxlc activity of the product against L-929 cell was 1. 0×107units/ml culture.     

THE PHENOTYPE OF MAST CELL IN PRIMARY ADENOID LIVER TUMOR OF RAT AND ITS RELATION TO TUMOR CELL

Mast cells in adenoid liver tumors of 32 rats induced with nitrosomorpholine were observed ultrastructurally, and among them, some were studied immunocytochemically via immunogold techniques. Data indicating that mast cells which located in tumor tissues presented positive expression of rat mast cell protein (RMCP) Ⅰ, Indicating origination from the mucosa mast cells, while those in the connective tissues around tumors were largely stained negatively with either RMCP Ⅰor RMCP Ⅰ antisera, with the exception of only a few cells showing positive RMCP Ⅰ staining. Ultrastructural observation showed that mast cells in tumon contacted closely with the tumor cells. Membranes of the intracytoplasmic granules in these mast cells were fusing together. The content inside the granules were discharged and spread along the intercellular space between the tumor cells. There was not any lesion observed uitrasructrually in the tumor cells contacting with the mast cells. The significance of mucosa mast cells in adenoi     

MALIGNANT HISTIOCYTOSIS: A STUDY ON CLINICOPATHOLOGICAL FEATURES AND CELL ORIGIN

Thirty- one autopsy cases previously diagnosed as malignant histiocytosis (MH) were studied by means of immunohistochemical staining. Antibodies detecting the formalin resistant epitopes on T- cells, B- cells and those of histiocyte/monocyte origin were used. It was shown that the malignant histiocytes reacted only to the cell markers derived from histlocyte/monocyte. and only a part of lymphocytes showed positive reaction to the T and B cell markers. It is suggested that the histiocyte/monocyte lineage is the possible origin of the malignant proliferating cells in MH. The clinicopathological features and the differentiation of MH from familial erythrophagocytic lymphohistiocytosis, virus-associated hemophagocytic syndrome and malignant lymphoma are described. The pathogenesis. the causes of death and the points for attention in the treatment of MH are also discussed.     

SURGICAL TREATMENT OF HUGE MALIGNANT MEDIASTINAL TUMOR

ＳＵＲＧＩＣＡＬＴＲＥＡＴＭＥＮＴＯＦＨＵＧＥＭＡＬＩＧＮＡＮＴＭＥＤＩＡＳＴＩＮＡＬＴＵＭＯＲＤｕａｎＤｅｐｕ；段德溥ＹａｎｇＫｅｘｉａｎ；杨可贤ＸｉｎＤｅｈｅ；信德和ＺｈａｎｇＳｈａｏｍｉｎｇ；张绍明ＺｈａｎｇＨａｎｇ；张珩ＲｅｎＹｏｕＪｕｎ；任...     

EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND BASIC FIBROBLAST GROWTH FACTOR IN HUMAN NON-SMALL CELL LUNG CANCER AND THEIR CLINICAL SIGNIFICANCE

Solid tumors contain tumor cells and vascular systems[1]. The growth and metastasis of solid tumors beyond 1-2mm in diameter depends on neovascularization. So the study of neovascularization helps to explain the biologic behavior of tumor. Angiogenesis is a complicated process mediated by a variety of angiogenic factors, which include vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF). VEGF is an endothelial cell-specific mitogen with a pivotal role and may a…     

TRICHOSTATIN A INHIBITS PROLIFERATION, INDUCES APOPTOSIS AND CELL CYCLE ARREST IN HELA CELLS

Objective： The histone deacetylase inhibitors （HDACIS） have been shown to inhibit cancer cell proliferation, stimulate apoptosis, an induce cell cycle arrest. Our purpose was to investigate the antiproliferative effects of a HDACI, trichostatin A （TSA）, against human cervical cancer cells （HeLa）. Methods： HeLa cells were treated in vitro with various concentrations of TSA. The inhibitory effect of TSA on the growth of HeLa cells was measured by MTT assay. To detect the characteristic of apoptosis chromatin condensation, HeLa cells were stained with Hoechst 33258 in the presence of TSA. Induction of cell cycle arrest was studied by flow cytometry. Changes in gene expression of p53, p21wafl and p27Kipl were studied by semiquantitative RT-PCR. Results： TSA inhibited cell growth in a time- and dose-dependent manner. Hoechst 33258 staining assay showed that TSA induced apoptosis. Cell cycle analysis indicated that treatment with TSA decreased the proportion of cells in S phase and increased the proportion of cells in G0/G1 and/or G2/M phases of the cell cycle. This was concomitant with overexpression of genes related to malignant phenotype, including an increase in p53, p21wall and p27Kipl. Conclusion： These results suggest that TSA is effective in inhibiting growth of HeLa cells in vitro. The findings raise the possibility that TSA may prove particularly effective in treatment of cervical cancers.     

GENE EXPRESSION OF GROWTH FACTOR RECEPTORS AND NEURONAL MARKERS IN NEURO BLASTOMA CELL LINES

Gene expression of nerve growth factor receptor (NGFR), epidermal growth factor receptor (EGFR), chromogranin A (CGA) and neuropetide Y (NPY)in 4 aeuroblsstoma cell lines without N-myc amplification was studied by wins Northern blot technique, N type cells expressed more NGFR mRNA than S type cells and have only little or no EGFR expression. S type cells had stronger expression of EGFR mRNA than that of N type cells accompanying with only less or even no NGFR expression. The results Indicated that difference of gene expression of theae growth factor receptors might be due to the various of tumor cell differetiation. Celli differentiating toward neurons gave more NGFR expression and cells prepared to be differentiating toward other direction might give more EGFR gene expression.Various gene expression of CGA and NPY In neuroblsstoma cell lines might be due to the presence of different stages of tumor cell differentiation and NGF only Induced differentiation of those neuroblastoma cells ready to be differ     

T－LYMPHOCYTE MEDIATED TUMOR CELL DESTRUCTIONIN VIVO ASSOCIATING WITH A SPECIFICFEATURE OF APOPTOSIS

Ｔ－ＬＹＭＰＨＯＣＹＴＥＭＥＤＩＡＴＥＤＴＵＭＯＲＣＥＬＬＤＥＳＴＲＵＣＴＩＯＮＩＮＶＩＶＯＡＳＳＯＣＩＡＴＩＮＧＷＩＴＨＡＳＰＥＣＩＦＩＣＦＥＡＴＵＲＥ　ＯＦ　ＡＰＯＰＴＯＳＩＳＹｕＤａ鱼达；ＹａｎｇＨｕａ杨骅；ＺｈｅｎｇＳｈｕ郑树；ＷａｎｇＸｉａ...     

MULTICELLULAR-MEDIATED EXPRESSION OF P-GP AND MRP AND RELATIONSHIP WITH CELL CYCLE PROFILES IN HUMAN OVARIAN CANCER SK-OV-3ip1 MULTICELLULAR AGGREGATES

Clinical multidrug resistance to chemotherapeutic agents is a major obstacle to treatment of patients with advanced epithelial ovarian cancer. A number of possible mechanisms have been put forward to account for drug resistance[1]. However, most of these proposed mechanisms are based largely on the study of drug-resistant variants isolated from tumor cell lines exposed to various classes of drugs in monolayer tissue culture. The impact of such mechanisms on clinical drug resistance remains spe…     

A CLINICAL STUDY OF THE HEMATOPOIETIC STEM CELL TRANSPLANTATION IN 112 PATIENTS WITH LEUKEMIA AND OTHER MALIGNANT DISEASES

Hematopoieticstemcelltransplantation(HSCT)hasbeenmakingremarkableprogressinrecentyearsandhasbecomeanimportantmeasureforthetreatmentofleukemiaandsomeothermalignancies.From1983toOctober,1995,112casesofleukemiaandothermalignanciesweretreatedwithHSCTinourhospital,whichisreportedasfollows.MATERIALSANDMETHODSPatientsOnehundredandtWelvecaseswereallinpatients,including58caseswithacutemyeloidleukemia(AML),33caseswithacutelymphoblasticleukemia(ALL),12caseswithchronicmyelogenousleukemia(CM…     

PREPARATION AND CYTOLYTIC TUMOR ACTIVITY OF OSTEOSARCOMA TUMOR INFILTRATING LYMPHOCYTES IN VITRO

In this study, the isolation, purification and differentiation of tumor-lnflltratlng lymphocytes (TIL) from 6 fresh osteosarcoma specimens were achieved by discontinuous density gradient centrifugation. One specimen of the osteosarcoma TIL were enlarged in IL-2 for long time in vitro, reaching 28 days and their cytolytic activity against different tumor cell lines was Investigated. The experimental results indicated that the preparation of osteosarcoma TIL adopted by the mechanical means was simple, having higher purifity, keeping higher effects on killing NK- sensitive tumor cell lines and NK-insensitive tumor cell lines as well as rapid proliferation in vitro cultured in IL-2.     

THE ULTRASTRUCTURAL STUDY OF NON－HODGKIN＇S LYMPHOMA CELL，CHRONIC LYMPHOCYTIC AND HAIRY CELL LEUKEMIA

ＴＨＥＵＬＴＲＡＳＴＲＵＣＴＵＲＡＬＳＴＵＤＹＯＦＮＯＮ－ＨＯＤＧＫＩＮ＇ＳＬＹＭＰＨＯＭＡＣＥＬＬ，ＣＨＲＯＮＩＣＬＹＭＰＨＯＣＹＴＩＣＡＮＤＨＡＩＲＹＣＥＬＬＬＥＵＫＥＭＩＡＧｕｉＷｅｉ；归薇ＺｈａｎｇＱｉａｏｈｕａ；张巧花ＺｈｅｎｇＹｕｐｉｎｇ...     

EFFECTS OF S86019, AN ACTIVE COMPONENT FROM PURALIA LOB AT A, ON CELL DIFFERENTIATION AND CELL CYCLE TRAVERSE OF HL-60 CELLS

The effects of S86019, an active component from Puralia lobata, on the induction of cell differentiation and cell cycle traverse of HL-60 cells were described. It was shown that cell proliferation of HL-60 cells was inhibited by S86019 in vitro. Under the action of S86019 the HL-60 cells were induced to differentiate into metamyelocytes, myelocytes and much matured cells with banded or segmented nucleus. Flow cytometry demonstrated that the cell population of HL-60 cells was blocked at G1 phase which resulted in the elevation of percentage of G1 cells and decrease of percentage of cells in S phase. Experimental results demonstrated that S86019 is an active inducer of cell differentiation in HL-60 cells.     

PRIMARY NEUROENDOCRINE CARCINOMA OF THE SKIN (NERKEL CELL TUMOR)

ＰＲＩＭＡＲＹＮＥＵＲＯＥＮＤＯＣＲＩＮＥＣＡＲＣＩＮＯＭＡＯＦＴＨＥＳＫＩＮ（ＭＥＲＫＥＬＣＥＬＬＴＵＭＯＲ）ＬｕＮｉｎｇ；吕宁；ＬｉＪｉｎｇｘｉａｎ；李竞贤（ＤｅｐａｒｔｍｅｎｔｏｆＰａｔｈｏｌｏｇｙ，ＣａｎｃｅｒＩｎｓｔｉｔｕｔｅ，Ｃｈｉｎｅｓ...     

INTERACTION OF CISPLATIN WITH MEMBRANES OF RAT EHRLICH ASCITES TUMOR CELL

ＩＮＴＥＲＡＣＴＩＯＮＯＦＣＩＳＰＬＡＴＩＮＷＩＴＨＭＥＭＢＲＡＮＥＳＯＦＲＡＴＥＨＲＬＩＣＨＡＳＣＩＴＥＳＴＵＭＯＲＣＥＬＬ￥ＣｈｅｎＢａｏｗｅｉ；陈宝卫；ＷａｎｇＫｕｉ；王夔（ＤｅｐａｒｔｍｅｎｔｏｆＩｎｏｒｇａｎｉｃＣｈｅｍｉｓｔｒｙ，Ｎａｔｉ...     

EFFECT OF ASCORBIC ACID ON DNA SYNTHESIS,INTRACELLULAR ACCUMULATION OF ADM AND ADM RESISTANCE OF TUMOR CELL LINES

ＥＦＦＥＣＴＯＦＡＳＣＯＲＢＩＣＡＣＩＤＯＮＤＮＡＳＹＮＴＨＥＳＩＳ，ＩＮＴＲＡＣＥＬＬＵＬＡＲＡＣＣＵＭＵＬＡＴＩＯＮＯＦＡＤＭＡＮＤＡＤＭＲＥＳＩＳＴＡＮＣＥＯＦＴＵＭＯＲＣＥＬＬＬＩＮＥＳＸｉｅＺｕｏｆｕ谢佐福ＬｉｎＸｉａｎｄｏｎｇ林贤东Ｚｈｏ...     

ESTABLISHMENT OF A CELL MODEL CONTAINING A DEFECTIVE FACTOR Ⅸ GENE

用电穿孔法将质粒Ａ（ｐＫＧ５－ｈＦⅨΔ３’ＮｅｏΔ５’）和选择基因（ｐＩＣ１９Ｒ／ＭＣ１－ＴＫ）同时向体外培养的ＬＴＫ细胞内转移，再借助ＨＡＴ培养基选择系统和ＰＣＲ技术筛选出了五个细胞基因组上转入了质粒Ａ的ＬＴＫ细胞克隆，并建立了五个带有缺陷Ⅸ因子基因的ＬＴＫ细胞株，即凝血因子Ⅸ定点整合细胞模型