Comparison of long-term quality of life between Billroth-I and Roux-en-Y anastomosis after distal gastrectomy for gastric cancer: a randomized controlled trial

Background: The results of studies comparing Billroth-I (B-I) with Roux-en-Y (R-Y) reconstruction on the quality of life (QoL) are still inconsistent. The aim of this trial was to compare the long-term QoL of B-I with R-Y anastomosis after curative distal gastrectomy for gastric cancer.Methods: A total of 140 patients undergoing curative distal gastrectomy with D2 lymphadenectomy in West China Hospital, Sichuan University from May 2011 to May 2014 were randomly assigned to the B-I group (N = 70)...     

腹腔液中层粘连蛋白含量与胃癌病理及腹膜转移相关因素的关系

目的探讨胃癌腹腔液中层粘连蛋白含量与其病理生物学行为及腹膜转移相关因素的关系.方法术中收集50例胃癌和10例胃良性病变的腹水或腹腔冲洗液,采用放射免疫技术检测上清液中层粘连蛋白、CEA蛋白(p-CEA)含量,同时进行腹腔冲洗细胞学(peritoneal lavage cytology,PLC)和病理学检查.结果层粘连蛋白含量与胃癌组织学类型、生长方式、浸润深度及淋巴结转移呈正相关.PLC和p-CEA阳性组层粘连蛋白含量均高于阴性(P＜0.05);肉眼腹膜转移的10例中层粘连蛋白均显著升高.全组层粘连蛋白含量升高者占68%,明显高于p-CEA(44%)和PLC(34%)的阳性率.结论腹腔液中的层粘连蛋白是反映胃癌生物学行为的分子标志物,与胃癌腹膜转移密切相关,其预测腹膜亚临床转移的灵敏性优于p-CEA和PLC.     

免疫磁珠技术检测胃癌患者腹腔微小转移的研究

目的 研究免疫磁珠技术检测胃癌患者腹腔微小转移的方法及临床意义.方法 收集30例胃癌患者术中的腹腔冲洗液,分别进行免疫磁珠技术及常规腹腔冲洗液细胞学检查,检测腹腔内脱落癌细胞.结果 免疫磁珠的阳性检出率(63.3%)高于常规腹腔冲洗液细胞学检查(40.0%)(P＜0.05);免疫磁珠的阳性检出率与TNM分期密切相关(P＜0.01).结论 免疫磁珠技术是一种有效检测肿瘤细胞的方法,可以从胃癌患者腹腔冲洗液中分离出癌细胞,监测微转移的存在,有助于临床对胃癌的诊断和治疗.     

腹腔镜与开腹手术对早期胃癌根治术的临床疗效

目的比较腹腔镜和传统开腹胃癌根治术,分析腹腔镜胃癌根治术治疗早期胃癌的临床疗效。方法回顾我院2005年1月～2011年12月期间的56例早期胃癌根治术患者,其中腹腔镜组32例,开腹组24例,比较两组的临床观察指标。结果腹腔镜术32例患者均在腹腔镜下完成胃切除和淋巴结清扫,无中转开腹病例。术中出血量分别为(152.6±184.3)mL和(261.3±211.1)mL,腹腔镜组明显低于开腹组(P<0.05);术后肛门平均排气时间分别为3 d和4 d,腹腔镜组明显短于开腹组(P<0.01)。结果腹腔镜胃癌根治术治疗早期胃癌安全有效,具有创伤小、出血少、术后恢复快等微创优点。     

Expression of survivin mRNA in peritoneal lavage fluid from patients with gastric carcinoma

Background Peritoneal dissemination is the most common pattern of metastasis in advanced gastric carcinoma with serosal invasion. In the present study, we reported the clinical relevance of a new diagnostic method involving RT-PCR, using survivin as the target gene, for the detection of free cancer cells in peritoneal washes. Methods Intraoperative peritoneal washes were obtained from 48 patients who underwent surgery for gastric cancer. RT-PCR analysis with primers specific for survivin and conventional cytologicalexaminations were both performed. Results Survivin mRNA was not detected in any peritoneal wash samples from patients with benign disease, but was detected in 28 of 48 samples taken from patients with gastric cancer and in all metastastic nodules. Survivin expression in the peritoneal cavity significantly correlated with depth of cancer invasion, lymph node metastasis, and TNM stage. There were 92% of clinically evident peritoneal metastasis cases showed detectable survivin expression. The combination of survivin RTPCR and cytological examination yielded positive results in 66.7% (32/48) of patients with gastric cancer, much higher than the results produced by cytological method alone. Conclusions Survivin mRNA detected in peritoneal lavage fluid might indicate the presence of free cancer cells in the peritoneal cavity. The high sensitivity of the RT-PCR-based survivin assay suggests that survivin serves as a molecular marker for detecting peritoneal micrometastasis. Its ubiquitous expression in peritoneal cancer cells and metastatic nodules also suggests a promising future therapeutic strategy based on survivin inhibition for cases of gastric cancer involving peritoneal metastasis.     

腹腔镜辅助胃癌D2根治术临床研究

Background Laparoscopic assisted radical gastrectomy is gaining acceptance for treating early gastric cancer. However, few reports concerning the effectiveness of patients who have undergone laparoscopic assisted D2 distal gastrectomy (LADG) for advanced gastric cancer or data comparing the results to those obtained after open distal gastrectomy (ODG) are yet available. The aim of this study is to evaluate the method, feasibility and clinical result of laparoscopic assisted D2 radical distal gastrectomy for advanced gastric cancer. Methods A retrospectively study was performed comparing LADG and ODG for advanced gastric cancer. 78 patients who underwent LADG were compared to 90 patients who underwent ODG in terms of pathologic findings, operative outcome, complications. Results There was no conversion to open surgery in LADG group and no postoperative mortality in all patients. There were no significant difference between LADG and ODG in operative time (245±35 vs 220±20 min), complication rate (7.7% vs 10.0%), and number of lymph nodes (23.5±6 vs 21.0±7.5), while the blood loss was less after LADG (110±25 vs 196±30 mL, P＜0.05). The time of postoperative flatus and postoperative hospital stay was shorter after LADG (73±8.5 vs 102±10.5h, and 8.6±1.2 vs 12.1±2.5 d, P＜0.05, respectively). Conclusion Laparoscopic assisted D2 radical distal gastrectomy for advanced gastric cancer is feasible, safe, and minimally invasive.     

胃癌腹腔游离癌细胞的研究进展

腹膜是胃癌常见的复发和转移部位,腹膜复发和转移是影响胃癌患者预后的重要因素.作为腹膜转移的早期状态,腹腔游离癌细胞的检测是预测腹腔复发、估计预后、指导治疗的可靠指标.文章就胃癌腹膜游离癌细胞的研究进展进行综述.     

胃癌腹腔冲洗液肝素酶和存活素的相关研究

目的探讨检测胃癌腹膜转移的方法。方法收集50例胃癌患者及8例胃良性病变患者的腹腔冲洗液。采用流式细胞术（FCM）检测胃癌患者术中腹腔冲洗液肝素酶（HPA）和存活素（Survivin）的表达情况,并采用薄层液基细胞制片术进行腹腔冲洗液细胞学（PLC）检查。结果 50例胃癌患者腹腔冲洗液中HPA阳性表达为52.0%（26/50）;Survivin阳性表达率58.0%（29/50）,HPA和Survivin联合检测阳性率为68.0%（34/50）,两者阳性率皆高于PLC 22.0%的阳性率（P〈0.01）;HPA的阳性率与浸润深度、组织分化程度以及TNM分期呈正相关;Survivin的阳性率与肿瘤浸润深度、组织分化程度、淋巴结转移及TNM分期呈正相关。8例良性病变患者腹腔冲洗液中HPA和Survivin无阳性表达。结论 （1）腹腔冲洗液HPA和Survivin的检测可作为判断肿瘤恶性程度和预后的一项指标。（2）FCM方法检测腹腔冲洗液HPA和Survivin可能成为临床预测胃癌腹膜转移的一种方法。     

腹腔镜与开腹胃癌根治术治疗进展期胃癌的临床对比评价

目的探讨腹腔镜与开腹胃癌根治术治疗进展期胃癌的临床疗效。方法选取于2015年1月1日~2017年6月30日来我院就诊的进展期胃癌患者70例作为研究对象,依照入院次序进行分组,每组均35例,其中常规组采取开腹胃癌根治术治疗,实验组采取腹腔镜胃癌根治术治疗,将两组的相关参数进行分析对比。结果实验组手术耗时长于常规组,但实验组的出血量、肛门排气时间、切口长度、下床时间、术后住院时间、首次进流食时间相比常规组均明显较低(P0.05);两组的淋巴结清扫数量与近远端切缘距离比较无明显差异(P0.05);实验组与常规组的并发症发生率分别为5.71%与22.86%(P0.05)。结论对进展期胃癌患者采取腹腔镜胃癌根治术进行治疗效果显著,对患者造成的创伤较小,出血量较少,住院时间较短,利于术后身体恢复,值得采纳。     

腹腔镜辅助下胃癌根治术的临床应用

目的探讨腹腔镜辅助下胃癌根治术的方法及临床应用价值。方法回顾性分析2010年1月至2011年4月重庆市开县人民医院18例接受腹腔镜辅助下胃癌根治术患者的淋巴结清扫数目、术后胃肠功能恢复时间、术中出血量、手术时间、术后卧床时间、术后住院时间,并与同期30例开腹手术比较。结果 18例患者均成功接受腹腔镜辅助下胃癌根治术,腹腔镜组与开腹组的平均淋巴结清扫数目分别为(49.6±10.7)枚及(47.8±8.5)枚;术后胃肠功能恢复时间分别为(62.1±11.2)h及(81.6±16.8)h;术中出血量分别为(224.3±33.0)ml及(217.7±25.8)ml;手术时间分别为(274.7±17.9)min及(201.3±9.8)min;术后卧床时间分别为(28.9±4.4)h及(62.1±7.3)h;术后住院时间分别为(6.17±0.99)d及(10.9±0.77)d。结论与同期开腹手术比较,腹腔镜辅助下胃癌根治术具有手术创伤小、术后恢复快的优点,能够达到与开腹手术相当的根治效果。     

腹腔镜与开腹远端胃癌根治术疗效及对凝血功能影响的对比研究

目的：对比腹腔镜与开腹远端胃癌根治术的疗效及对凝血功能的影响。方法选取行腹腔镜远端胃癌根治术的患者56例（观察组）及同期开腹远端胃癌根治术患者56例（对照组）。对两组患者的术中及术后情况、淋巴结清扫数目、并发症等进行比较分析,并检测比较两组患者术前、术后24h凝血酶原国际标准化值（INR）、凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、D-二聚体（D- D）以及纤维蛋白原（FIB）水平。结果观察组手术时间长于开腹组,而术中出血量、切口长度、术后首次肛门排气时间、术后镇痛药使用次数、住院时间均少于开腹组,差异均有统计学意义（均P＜0.01）。与手术前比较,仅观察组患者PT显著延长,差异有统计学意义（P＜0.05）;与对照组比较,观察组PT显著延长,差异有统计学意义（P＜0.05）。与术前相比,两组患者术后FIB及D- D体均明显增高,差异均有统计学意义（均P＜0.05）;观察组患者术后FIB及D- D较对照组明显增高,差异有统计学意义（P＜0.05）。观察组术后并发症发生率明显低于对照组（10.7%、14.3%）,差异有统计学意义（P＜0.05）。结论腹腔镜远端胃癌根治术具有安全、术后恢复快等优点,且在淋巴结清扫方面能达到与开腹手术相同的效果,但其可导致患者处于高凝状态,可能发生血栓性并发症,应予以重视。