Antioxidant and hepatoprotective effects of Orthosiphon stamineus Benth. standardized extract

Orthosiphon stamineus (OS), Benth. (Lamiaceae) is widely used in Malaysia for treatments of various kidney and liver ailments. In the experiment, DPPH* radicals scavenging, Fe(3+)-induced lipid peroxidation inhibiting activities and trolox equivalent antioxidant capacity (TEAC) of methanol/water extract of Orthosiphon stamineus (SEOS) were determined. The results indicated that SEOS exhibited antioxidant, lipid peroxidation inhibition and free radical scavenging activities. The hepatoprotective activity of the SEOS was studied using CCl(4)-induced liver toxicity in rats. The activity was assessed by monitoring liver function tests through the measurement of alanine transaminase (ALT) and aspartate transaminase (AST). Furthermore, hepatic tissues were also subjected to histopathological studies. Pretreatment of SEOS (125, 250, 500 and 1000 mg/kg p.o.) dose-dependently reduced the necrotic changes in rat liver and inhibited the increase of serum ALT and AST activities. The results of the present study indicated that the hepatoprotective effect of Orthosiphon stamineus might be ascribable to its antioxidant and free radical scavenging property.     

鱼腥草减轻糖尿病大鼠肾损害的作用机理研究

目的研究鱼腥草蒸馏液对2型糖尿病大鼠尿蛋白、尿白蛋白(Alb)、肾组织结缔组织生长因子(CTGF)、脂联素、单核细胞趋化蛋白-1(MCP-1)的影响。方法采用高糖高脂饲料喂养,配合小剂量链脲佐菌素(STZ)一次性腹腔注射造成2型糖尿病大鼠模型,经鱼腥草蒸馏液灌胃治疗8周后,观察大鼠24h尿蛋白、Alb、肾组织CTGF、脂联素、MCP-1等指标的变化。结果治疗8周后,鱼腥草蒸馏液能明显改善STZ大鼠多饮、多食和消瘦,降低24h尿蛋白、Alb,减少肾组织CTGF和MCP-1表达。但对脂联素的影响不显著。结论鱼腥草蒸馏液对糖尿病大鼠肾脏病变有一定的改善,其作用机制可能与降低尿蛋白、Alb及肾脏CTGF和MCP-1的表达有关。     

芪蛭降糖片对链脲佐菌素诱导糖尿病大鼠的心脏保护作用

目的观察芪蛭降糖片对链脲佐菌素(STZ)诱导糖尿病大鼠的心脏保护作用,并探讨其可能的作用机制。方法采用尾静脉注射链脲佐菌素(STZ,60 mg·kg^-1)法建立糖尿病大鼠模型。将90只Wistar大鼠随机分为正常组、模型组、芪蛭降糖片组(780 mg·kg^-1)、盐酸二甲双胍片组(200 mg·kg^-1)和格列本脲片组(1.5 mg·kg^-1);灌胃体积为10 mL·kg^-1,每天灌胃给药1次,连续3个月。检测大鼠的空腹血糖值、饮水量、饮食量、尿量、体质量,以及心电图、血压变化情况。采用网络药理学方法预测芪蛭降糖片对糖尿病性心脏病的作用机制;苏木素-伊红(HE)染色法观察心脏组织病理学变化;免疫组织化学法检测心脏组织转化生长因子β1(TGF-β1)、核转录因子κB(NF-κB)及半胱氨酸蛋白酶3(Caspase-3)的表达;Western Blot法检测心脏组织I型胶原蛋白(Collagen I)与基质金属蛋白酶抑制因子2(TIMP-2)的表达。结果①网络药理学分析表明,芪蛭降糖片主要通过黄芪异黄烷甙、西托糖苷、黄精皂苷、槲皮素、水蛭素、京尼平苷等主要成分,作用于ADRA1D、INSR、ADRA1A、PTGS2、EDNRA等关键靶点,主要调节胰岛素抵抗、环磷酸鸟苷-环磷酸鸟苷依赖蛋白激酶G(cGMP-PKG)、NF-κB等信号通路,以及通过一氧化氮生物合成、G蛋白偶联受体通路、血管收缩等过程阻止心脏损伤。②与正常组比较,模型组大鼠在第10、40、90天的饮水量、饮食量、尿量及空腹血糖值均明显升高(P <0.01),体质量明显降低(P <0.05,P <0.01);第40、90天的血压明显升高(P <0.05,P <0.01),心电图表现出心律不齐,主波异常现象,第90天的左心室上升达最大速率所需时间明显延长(P <0.01);心脏组织的TGF-β1、NF-κB、Caspase-3、Collagen I及TIMP-2蛋白表达水平明显升高(P <0.05,P <0.01)。与模型组比较,芪蛭降糖片组在第10、40、90天的饮水量、饮食量、尿量均明显降低(P <0.05,P <0.01),体质量明显增加(P <0.05,P <0.01);第40、90天的空腹血糖值明显降低(P <0.01);第40、90天的血压明显降低(P <0.05),心电图在第40天未表现出心律不齐、主波异常现象,且第90天的左心室上升达最大速率所需时间明显缩短(P <0.05);大鼠心脏组织的TGF-β1、NF-κB、Caspase-3、Collagen I及TIMP-2表达水平均明显降低(P <0.05,P <0.01)。结论芪蛭降糖片可改善糖尿病造成的多饮、多食、多尿与消瘦现象,并对糖尿病所致心脏损伤有保护作用,可能与其抑制心脏组织Collagen I、TIMP-2、TGF-β1、NF-κB、Caspase-3蛋白表达,从而抑制炎症、心肌纤维化有关。     

灰兜巴抗糖尿病作用的实验研究

目的 考察灰兜巴的抗糖尿病作用,并初步了解其具有降血糖作用的物质基础.方法 将灰兜巴先用75%乙醇溶液提取,再用热水提取得到水提部分(HDB-4);醇提部分浓缩除去乙醇后,用石油醚、醋酸乙酯分别萃取,得到石油醚萃取部分(HDB-1)、醋酸乙酯萃取部分(HDB-2)和醇提萃取剩余部分(HDB-3).以给予大鼠高糖高脂饮食配合小剂量腹腔注射链脲佐菌素(STZ)的方法建立2 型糖尿病大鼠模型,以消渴丸为阳性对照,观察上述4种样品的降血糖作用.结果 HDB-1给药14d后,空腹血糖值明显降低,与模型组相比,具有统计学意义(P＜0.05),其余3个部分均有一定的降低空腹血糖趋势,但无统计学差异.结论 灰兜巴具有一定的抗糖尿病作用,并且石油醚萃取部分的效果最好.     

镇痛灵对大鼠实验性糖尿病降血糖作用及机制的研究

中药剂制镇痛灵25g／kg腹腔注射12d，对正常大鼠和糖尿病大鼠均有降血糖作用。作用机制可能与其促进四氧嘧啶引起的大鼠胰岛β细胞损伤的修复和胰岛素的分泌，增加肝糖元合成，改善耐糖功能有关。     

Studies on diuretic and hypouricemic effects of Orthosiphon stamineus methanol extracts in rats

AIM OF THE STUDY: Orthosiphon stamineus (Labiatae) is a traditional folk medicine widely used in Southeast Asia for the treatment of several kidney disorders, gout and as a diuretic. This study was conducted to examine the diuretic and hypouricemic effects of Orthosiphon stamineus leaf extracts. MATERIALS AND METHODS: The diuretic effect of different methanol extracts was examined by treating different groups of Sprague-Dawley rats with single (2g/kg) oral doses of methanol and methanol:water (1:1) extracts. Hydrochlorothiazide (10mg/kg) was used as positive control in acute study. Methanol and methanol water (1:1) extracts at 0.5 g/kg were administered for a period of 7 consecutive days. Cumulative urine volume and electrolytes (Na+ and K+) concentrations at different time intervals were measured. On the other hand, hypouricemic activity of methanol:water extract (1:1) was experimented using different oral single doses (0.25, 0.5, 1 and 2g/kg). Allopurinol was used as positive control. Uric acid concentration in serum was analyzed by using RP-HPLC at 280 nm. RESULTS: Sodium and potassium excretion increased significantly (p<0.05 and <0.01) in the first 8h of treatment with a single dose (2g/kg) of the extracts in a pattern comparable to that of the known diuretic hydrochlorothiazide. Meanwhile, repeated administration of 0.5 g/kg methanol:water (1:1) extract showed a significant increase in urine volume (from day 3 to day 7) (p<0.01) and electrolytes excretion (Na+ and K+) from day 2 to day 7 (p<0.05 and <0.01). On the other hand, 0.5, 1 and 2g/kg of methanol:water (1:1) extract and the standard allopurinol reduced the serum urate level in hyperuricemic rats at hour 6. CONCLUSION: These results provided an evidence of the high tendency of methanol:water (1:1) extract of Orthosiphon stamineus towards diuretic and hypouricemic effects in rats.     

槲皮素对链脲佐菌素诱导糖尿病大鼠氧自由基和一氧化氮的影响

目的:观察槲皮素对糖尿病大鼠超氧化物歧化酶(SOD),丙二醛(MDA),一氧化氮(N0)及一氧化氮合酶(NOS)的影响,分析槲皮素对糖尿病大鼠的肾脏保护机制.方法:采用一次性腹腔注射链脲佐菌素建立糖尿病模型.将大鼠随机分为3大组,每组再设2个小组(n=8):正常组(A1,A2),糖尿病模型组(B1,B2),槲皮素治疗组(C1,C2),分别于槲皮素(50 mg·kg-1·d-1)ig治疗6周和12周后处死取双肾,计算各组大鼠肾脏肥大指数;一肾用苏木精-伊红染色,光镜下观察肾小球病理形态学变化;另一肾测定其内SOD活性、MDA、NO含量及NOS活力的变化.结果:槲皮素治疗12周肾脏肥大指数(6.99±0.58) ×10-3,与模型组(8.92±0.66) ×10-3相比明显降低(P＜0.05).槲皮素治疗6周和12周肾组织SOD活性(165.31±14.89),(148.33±13.22) U· mL-1,与模型组(149.02±17.21),(121.32±15.33)U· mL-1相比明显升高(P＜0.05);MDA含量(17.89 ±2.88),(19.46 ±3.02)μmo1· L-1,NOS活性(15.13 ±2.89),(17.65±2.46) ng· L-1,NO含量(0.42 0.12),(1.41±0.48) μmol· L-1,与模型组MDA含量(19.68±3.01),(21.12 ±2.99)μmol· L-1,NOS活性(18.23 ±2.42),(21.22±3.01) ng· L-1,NO含量(0.73 ±0.25),(1.91±0.35) μmol· L-1相比明显下降(P＜0.01);且明显改善肾小球病理变化.结论:槲皮素可通过增加氧自由基的清除,抑制NOS的活性及NO的含量保护糖尿病肾脏.     

灯盏花素对2型糖尿病大鼠肾脏肥大的抑制作用与机制研究

目的研究灯盏花素（Bre）对2型糖尿病（T2DM）大鼠肾脏肥大的抑制作用与机制。方法建立T2DM大鼠模型,给予灯盏花素腹腔注射治疗,测定大鼠血糖、血脂、总胆固醇、血清胰岛素水平、肾脏肥大指数和单根近端小管（PT）钠泵活性。结果与正常对照组比较,T2DM模型组血糖、血脂、总胆固醇水平都显著升高;胰岛素敏感指数（ISI）显著降低;6周组大鼠PT钠泵活性显著升高,但12周组大鼠PT钠泵活性显著降低;12周组肾脏肥大指数显著升高。与模型对照组比较,Bre治疗组血糖、血脂、总胆固醇水平都显著降低;6周组大鼠胛钠泵活性显著降低,但12周组大鼠PT钠泵活性显著升高;ISI显著升高;12周组肾脏肥大指数显著降低。结论Bre能抑制T2DM大鼠肾脏肥大,其机制可能与降低血糖、血脂和总胆固醇水平,升高ISI和稳定胛钠泵活性有关。     

复方三芪丹胶囊对糖尿病大鼠糖代谢的影响及其作用机制研究

目的:观察复方三芪丹胶囊对糖尿病大鼠糖代谢的作用及其作用机制。方法:通过链脲佐菌素制备大鼠高血糖模型,测定给药后糖尿病大鼠空腹血糖(FBG)、糖耐量、血清胰岛素(FINS)、肝糖原含量及SOD、MDA、TNF-a水平,评价复方三芪丹胶囊的效果。结果:复方三芪丹胶囊明显降低糖尿病模型大鼠FBG,改善模型大鼠糖耐量,升高FINS及肝糖原含量,提高SOD活性,降低MDA、TNF-a水平。结论:复方三芪丹胶囊通过调整糖代谢降低糖尿病大鼠血糖水平。     

水蛭对糖尿病大鼠肾脏病变的防治作用及机理探讨

目的:探讨水蛭对糖尿病大鼠肾脏病变的防治作用及其可能机理。方法:40只SD大鼠随机分成正常对照组、模型组与水蛭组。水蛭〔1.67g/(kg·d)〕混入普通饲料给药;实验第90、180天分别测24h尿蛋白量,尿微量白蛋白及尿NAG并经尾静脉取血测血清尿素氮(BUN)、肌酐(Scr)。实验结束时颈动脉取血测血浆一氧化氮(NO)、丙二醛(MDA)、全血谷胱甘肽过氧化物酶(GSH-PX)、组织型纤溶酶原激活物(t-PA)、纤溶酶原激活剂抑制物(PAI)等,并进行肾脏光镜及透射电镜观察。结果:模型组大鼠6个月时出现明显高凝、高粘现象,肾脏微血管病变比较明显。水蛭组3个月时尿微量白蛋白显著低于模型组(P<0.05),6个月时水蛭组24h尿蛋白显著低于模型组(P<0.001),尿NAG、TG、MDA等也有显著性差异,且高凝、高粘现象及微血管病变也得到明显改善。结论:水蛭虽不能完全保护或阻止糖尿病肾病的发生发展,但可延缓或减轻糖尿病大鼠肾脏病变的的发生发展。     

络通对不同病程糖尿病大鼠视网膜微血管形态学改变的影响

目的探讨络通对不同病程的糖尿病大鼠视网膜微血管形态学改变的防治作用。方法采用链脲佐菌素(65㎎/㎏)经大鼠腹腔一次性注射制备糖尿病模型,成模糖尿病大鼠随机分成糖尿病模型组、成模后即开始络通干预6个月组(络通干预组)和成模6个月后开始络通治疗3、6个月组(络通治疗1、2组)。分别按期摘取眼球,做普通病理及透射电镜观察,并利用LEICA-Q550IW计算机多功能真彩色病理图像分析系统定量分析视网膜微血管基底膜厚度。结果与6个月模型组比较,光镜和电镜观察下络通干预组视网膜微血管形态学改变明显减轻。9个月时,模型组大鼠视网膜微血管改变进一步加重;络通治疗3个月后,视网膜微血管改变较9个月模型组有所减轻,但病变仍在进行性加重。12个月时,模型组大鼠视网膜微血管形态学改变非常严重,络通治疗6个月后,整体微血管改变较12个月模型组明显减轻,与9个月模型组无明显差异。计算机图像分析显示络通干预组及络通治疗1、2组视网膜毛细血管基底膜厚度与同期模型组比较有非常显著性差异(P<0.01)。结论络通对糖尿病大鼠视网膜微血管病变具有较好的防治作用,且早期干预胜于后期治疗。     

葛根素注射液对糖尿病肾小球VEGF表达调节的实验研究

目的探讨葛根素注射液对糖尿病肾小球VEGF表达的影响。方法建立1型糖尿病大鼠模型,大鼠随机分正常组、模型组、葛根素注射液治疗组,治疗12周;观察治疗期间及治疗后大鼠血糖、内生肌酐清除率、24h尿蛋白排泄率,免疫组织化学检测肾小球VEGF表达。结果造模组大鼠均出现肾脏功能损害;葛根素注射液能降低糖尿病大鼠的24h尿白蛋白排泄率,增加内生肌酐清除率,VEGF表达显著降低。结论葛根素注射液通过调节肾小球VEGF表达对肾脏起到保护作用。     

Staminane- and isopimarane-type diterpenes from Orthosiphon stamineus of Taiwan and their nitric oxide inhibitory activity

From the MeOH extract of Taiwanese Orthosiphon stamineus, two new staminane-type diterpenes, staminols C (1) and D (2), and three new isopimarane-type diterpenes, orthosiphonone C (3) and D (4) and 14-deoxo-14-O-acetylorthosiphol Y (5), have been isolated together with 16 known diterpenes, orthosiphols A, B, D, K, M, N, O, X, and Y, nororthosiphonolide A, neoorthosiphol B, orthosiphonone A, secoorthosiphols B and C, 3-O-deacetylorthosiphol I, and 2-O-deacetylorthosiphol J. Their structures were determined on the basis of the spectroscopic data. All the newly isolated diterpenes exhibited dose-dependent inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophage-like J774.1 cells, and 2-O-deacetylorthosiphonone A showed the most potent activity, with an IC(50) value of 35.0 microM, comparable to that of the positive control N(G)-monomethyl-L-arginine (L-NMMA; IC(50), 35.7 microM).     

络通对糖尿病大鼠肾脏的保护作用及机理研究

目的探讨络通对糖尿病大鼠肾功能的作用及其可能机理.方法40只SD大鼠随机分成正常对照组、模型组与络通组.络通粉末(4g/kg·     

灯盏花素保护大鼠免受糖尿病肾病侵害的作用机制研究

目的：探究灯盏花素改善大鼠糖尿病肾脏侵害的作用机制。方法：将24只Wistar大鼠分为对照组(n=6)、模型组(n=9)和灯盏花素干预组(n=9)。通过链脲佐菌素诱导构建1型糖尿病Wistar大鼠模型。通过免疫比浊法检测血液糖化血红蛋白水平,结合尿液样本中白蛋白/肌酐比值,超氧化物歧化酶和丙二醛酶联免疫吸附试验检测结果,评估大鼠肾脏损伤情况。采用苏木精-伊红(HE)染色和透射电子显微镜观察大鼠肾皮质组织肾小球病理变化。使用TUNEL测定法检测肾脏细胞凋亡情况;通过蛋白质印迹法分析p-AMP活化蛋白激酶、p-糖原合酶激酶-3β(GSK-3β)、BCL-2-相关X蛋白(BAX)和裂解胱天蛋白酶-3(Cleaved Caspase-3)蛋白表达水平。结果：与模型组比较,灯盏花素干预组大鼠的血糖水平及糖化血红蛋白含量均明显降低(P<0.05);肾小球损伤和肾脏组织病变显著减轻(P<0.05);大鼠尿液中超氧化物歧化酶(SOD)水平显著升高,丙二醛水平显著降低(P<0.05);核因子E2相关因子2(Nrf2)/Keap1蛋白比值和血红素加氧酶-1(HO-1)蛋白水平均明显升高(...     

Antidiabetic and Anti-oxidative Effects of Honokiol on Diabetic Rats Induced by High-fat Diet and Streptozotocin

Objective To study the antidiabetic and anti-oxidative effects of honokiol(Hon)in Magnolia officinalis and its underlying molecular mechanism in diabetic rats induced by high-fat diet(HFD)and streptozotocin(STZ).Methods After ig administration with Hon[25,50,and 100 mg/(kg·d)]to diabetic rats for consecutive 10 weeks,the levels of blood glucose(BG),oral glucose tolerance(OGT),blood lipids including total cholesterol(TC),triglyceride(TG),high density lipoprotein-cholesterol(HDL-C),and low density lipoprotein-cholesterol(LDL-C),hepatic oxidative stress including the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),methane dicarboxylic aldehyde(MDA),and cytochrome P4502E1(CYP2E1)in diabetic rats were measured.Results Compared to the diabetic control rats,ig administration of Hon resulted in significant decrease in BG,TC,TG,and LDL-C levels in serum,as well as hepatic CYP2E1 activity and MDA content in diabetic rats,whereas the level of OGT and activities of hepatic CAT,SOD,and GSH-Px in diabetic rats were significantly increased.Conclusion Hon could alleviate hyperglycemia,hyperlipemia,hepatic oxidative damage,and insulin resistance in diabetic rats by inhibiting hepatic CYP2E1 activity.     

复方地归胶囊对糖尿病大鼠糖脂代谢紊乱的影响

目的探讨复方地归胶囊对糖尿病大鼠糖脂水平紊乱的影响。方法大鼠腹腔注射链佐菌素(50mg·kg-1)造成糖尿病模型,复方地归胶囊高、中、低剂量组分别以2.4,1.2,0.6g·kg-1d-1灌胃,连续90d,测定全血血糖、糖化血红蛋白以及血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)和一氧化氮(NO)、总抗氧化能力(T-AOC),并测定胰岛素和进行胰腺组织学观察。结果给药后2h复方地归胶囊可降低糖尿病大鼠血糖水平,给药90d后能降低血红蛋白糖化率,对TG、LDL-C、NO水平有明显的降低作用,对T-AOC有显著的升高作用;并促进胰岛细胞修复增生,对胰岛素的分泌也有一定促进作用。结论复方地归胶囊有降低糖尿病大鼠血糖、调节血脂紊乱、保护和修复胰岛细胞的作用。     

Effects of Orthosiphon stamineus aqueous extract on plasma glucose concentration and lipid profile in normal and streptozotocin-induced diabetic rats

The objective of this study was to investigate the effects of Orthosiphon stamineus Benth. aqueous extract on plasma glucose concentration and lipid profile in normal and streptozotocin-induced diabetic rats. The chemical screening of the extract showed phenolic compound and flavonoid content were 13.24+/-0.33 mg/g and 1.73+/-0.14 microg/g, respectively. In oral glucose tolerance test, the extract (0.2-1.0 g/kg) significantly decreased plasma glucose concentration in a dose-dependent manner in both normal and diabetic rats. The extract at 1.0 g/kg was most effective in decreasing plasma glucose concentrations and the response was closed to the result of glibenclamide (5 mg/kg). After repeated daily oral administrations of the extract (0.5 g/kg) for 14 days, the extract significantly reduced plasma glucose concentration in diabetic rats at days 7 and 14. By the end of the study, plasma triglyceride concentration was lower in the extract-treated diabetic rats than untreated ones. Furthermore, plasma HDL-cholesterol concentration was significantly increased in diabetic rats treated with the extract. In perfused rat pancreas, the extract did not increase insulin secretion in the presence of 5.5 mM glucose, but 100 microg/ml extract potentiated glucose-induced insulin secretion. Our findings suggested that Orthosiphon stamineus aqueous extract is effective for alleviating hyperglycemia and improving lipid profile in diabetic rats.     

Hypoglycaemic Effects of Opuntia ficus-indica Mill., Opuntia lindheimeri Engelm and Opuntia robusta Wendl. in Streptozotocin-induced Diabetic Rats

The hypoglycaemic activities of Opuntia ficus-indica, O. lindheimeri and O. robusta were investigated in streptozotocin-induced diabetic rats using enteral (oral) and parenteral (intraperitoneal and intravenous) routes of administration. When the aqueous extracts of the three Opuntia species were administred intraveneously, they produced a hypoglycaemic effect. However, when the extracts were administered orally, only O. lindheimeri produced a hypoglycaemic effect. A dilute aqueous (ammonium sulphate) solution of O. lindheimeri dialysate also produced a hypoglycaemic effect when administered intravenously. Intraperitoneal administations of all Opuntia species extracts including O. lindheimeri dialysate failed to produce hypoglycaemic effects. It appears that the concentration and/or potency of the active hypoglycaemic principle in Opuntia cacti is species-specific.     

糖肾平胶囊对糖尿病肾病大鼠肾脏保护作用及其对肾组织单核细胞趋化因子-1蛋白及mRNA表达的影响

目的:探讨糖肾平胶囊对糖尿病肾病大鼠肾脏保护作用及其对肾组织单核细胞趋化因子(MCP-1)蛋白及mRNA表达的影响。方法:雄性SD大鼠,50只,按体重均匀分出正常组10只,其余40只,腹腔注射链脲佐菌素造模。将造模成功的大鼠按血糖水平分为模型组、厄贝沙坦组、糖肾平胶囊组,用药12周。每周称量体重;4,8,12周后分笼收集24 h尿液检测24 h尿蛋白定量,处死大鼠行血液生化指标BUN、Scr、TG检测,肾组织行HE、Mallory染色,观察病理形态学改变,采用免疫组化、原位杂交分析肾组织MCP-1蛋白及mRNA的表达。结果:模型组大鼠摄食减少、精神不振、活动迟缓、尿量减少、体重减轻以及肾组织病理改变,而厄贝沙坦组和糖肾平胶囊组均有不同程度的改善,且糖肾平胶囊组优于厄贝沙坦组;正常组肾小管上皮细胞、肾间质细胞胞浆有少量MCP-1蛋白及mRNA表达;模型组则呈强阳性表达。糖肾平胶囊组MCP-1蛋白及mRNA的表达较模型组明显减少(P<0.05)。结论:糖肾平胶囊可能通过对肾组织MCP-1蛋白及mRNA表达下调而发挥治疗和预防糖尿病肾病的作用。