The performance of ELISA and dot-blot methods for the detection of crab flesh in heated and sterilized surimi-based products

Polyclonal anti-lobster arginine kinase antiserum was used as a probe to test for the presence of crab flesh (invertebrate tissues) in surimi-based products. Arginine kinase is a cytoplasmic enzyme present in many invertebrates and is absent from vertebrates. Antibodies against lobster arginine kinase interacted strongly with snow crab but they gave only a low response in molluscs such as limpet, squid and scallop. Surimi (washed fish mince) exhibited a slight residual immunobinding response. Arginine kinase could easily be detected in crab-supplemented surimi preparations with a correlation between the level of crab added to surimi and the level of immunological response. Using direct enzyme-linked immunosorbent assay and an immunodot procedure, the results showed that an addition of 10–25 g of crab flesh per kg surimi-based products could be detected even after a high sterilization process.     

Analysis of soyabean proteins in meat products: a review

The use of soyabean proteins as meat extenders has spread significantly due to the interesting nutritional and functional properties that are present in soyabean proteins. Together with these, health and economical reasons are the major causes for the addition of soyabean proteins to meat products. Nevertheless, despite the good properties associated to soyabean proteins, there are many countries in which the addition of these proteins is forbidden or in which the addition of soyabean proteins is allowed up to a certain extent. Thus, the need of analytical methods enabling the detection of added soyabean proteins in meat products is obvious. Microscopic, electrophoretic, immunologic, and chromatographic methods are the most widely used for this purpose. However, the detection of soyabean proteins in meat products presents difficulties related to the composition (meat species, meat quality, soyabean protein source, presence of other non-meat proteins, etc.) and the processing of the meat products, and, although these analytical methods have tried to overcome all these difficulties, there is still not a method enabling quantitative assessment of soyabean proteins in all kinds of meat products.     

Easy determination of the addition of soybean proteins to heat-processed meat products prepared with turkey meat or pork-turkey meat blends that could also contain milk proteins

The addition of non-meat proteins to processed meat products is limited by regulations. Therefore, this work has investigated the determination of added soybean proteins in commercial heat-processed meat products prepared with turkey meat or pork-turkey meat blends that could also contain milk proteins. The method consisted of extracting proteins from the meat products in a Tris-HCl buffer (pH 8) and analysing the extract by high-performance liquid chromatography with a linear gradient water-acetonitrile containing 0.05% (v/v) TFA. This method enabled the detection and quantitation of up to 0.08 and 0.28% (w/w), respectively, of soybean proteins (related to 6 g initial product) in these products. Satisfactory precision and recovery data were established. Accuracy was evaluated by a comparison of soybean protein contents determined by the proposed method and the existing AOAC official method based on an enzyme-linked immunosorbent assay (ELISA) from which no statistically significant differences were observed.     

速测盒法快速测定肉及肉制品中亚硝酸盐

目的 了解亚硝酸盐速测盒的可靠性,为现场监督执法及基层快速检测提供有力的技术支撑。方法采用速测盒方法检测亚硝酸盐标准溶液、肉及肉制品中亚硝酸盐添加情况,并与《GB 5009.33-2016食品中亚硝酸盐与硝酸盐的测定》第二法(盐酸萘乙二胺法)进行对比。结果速测盒对亚硝酸盐最低检出限可达到0.1 mg/L;检测样品时,速测盒与盐酸萘乙二胺法阴性符合率为97.8%,阳性符合率为100.0%。不同环境温度下,只需将反应时间控制在5 min以上,则不会对检测结果产生影响。样品经简单处理后,显色剂滴加到样品提取液中,混匀后反应3～5 min,即可观察结果。检测单个样品20 min内即可出结果。结论速测盒法具有快速、准确、方便、灵敏等特点,适用于肉及肉制品中亚硝酸盐现场定性分析。     

Production of a horse-specific monoclonal antibody and detection of horse meat in raw meat mixtures by an indirect ELISA

A stable hybridoma cell line (DD3) has been produced secreting a monoclonal antibody specific for horse muscle proteins. The DD3 monoclonal antibody (mAb) did not show significant cross-reactivity when tested against beef, chicken, pig and soya proteins or bovine caseins, gelatine and bovine serum albumin. The DD3 mAb was used in an indirect ELISA format for the detection of defined amounts of horse meat (10–500 g kg-¹) in beef meat mixtures. Immunorecognition of monoclonal antibodies adsorbed to horse meat adsorbed onto the ELISA plate was made with rabbit anti-mouse immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymic conversion of the substrate gave clear optical density differences when assaying mixtures of minced beef containing different amounts of horse meat.     

An improved,rapid, ELISA technique for detection of pork in meat products

The demand for analysts to determine the purity, quality and safety of food products is increasing. Adulteration of meat products with meat form other species is a particular problem. Determination of species of origin is possible using immunological assays. This paper reports a novel approach to the problem and discusses improvements and modifications of such immunologically based assays. These increase the sensitivity to 10 my g ^?1 of the adulterant species (pork) and permit automation and standardisation of the assay, increasing the reliability and rapidity compared with other methods.     

肉制品中6种人工合成色素的快速测定

目的:建立一种同时测定肉制品中柠檬黄、苋菜红、胭脂红、日落黄、诱惑红和酸性红6种人工合成色素的高效液相色谱法。方法:采用乙醇-氨溶液提取样品中的色素,经乙醚脱脂,用Agilent Poroshell 120 SB-C₁₈色谱柱分离,以乙腈-乙酸铵（0.02 mol/L）为流动相,二极管阵列检测器可变波长程序测定。结果:6种色素在浓度0¹0μg/mL均有良好的线性关系,线性系数均大于0.9999,加标回收率为70.33%⁹3.08%,检出限为0.12⁰.21 mg/kg,相对标准偏差均小于5%。结论:该方法操作简单、快捷,重现性好,灵敏度高,可同时测定肉制品中6种人工合成色素,适用于肉制品的快速检测。      

食品添加剂在肉制品保藏中的应用

介绍了肉制品保藏中常用的食品的添加剂的功能特性及其应用。     

发酵肉制品中生物胺及其产生菌株研究进展

生物胺是一类具有生物活性的含氨基小分子有机化合物,其广泛存在于蛋白丰富的食品中。生物体内适量生物胺具有增强代谢、促进生长等功能,但当含量达到临界值时,会产生诸多不良影响。存在大量微生物的食品中,生物胺的含量较高,同类发酵肉制品中生物胺的种类和含量也有很大不同,如发酵时间、贮藏时间和包装材料等都会对此产生影响。本文主要介绍生物胺的常见种类、生理作用,发酵肉制品中产生物胺的微生物种类、形成途径、菌株筛选方法及检测方法,旨在为今后发酵肉制品中生物胺的进一步研究提供帮助,以期为发酵肉制品的质量保障提供参考。     

几种豆类蛋白在肉制品中的应用

我国有丰富的豆类资源,这些豆类是廉价、优质的天然植物蛋白质来源。豆类蛋白具有良好的营养价值和功能特性,越来越多地被应用于肉制品加工中,不仅能提高产品的出品率,改善产品的口感,而且能够大大地降低肉制品的生产成本。本文对大豆蛋白的特点、分类以及几种大豆蛋白在肉制品中的应用研究进行了综述、同时也简单介绍了豌豆蛋白、芸豆蛋白、鹰嘴豆分离蛋白、黑豆蛋白和绿豆蛋白等几种不同豆类蛋白在肉制品中的应用研究进展,为豆类加工和肉制品产业发展提供参考。     

低盐低脂功能性肉制品的研究进展

我国是世界上肉制品生产的第一大国,同时也是肉制品消费总量最多的国家。近年来大量研究表明过量食用红肉及加工肉制品会增加罹患慢性疾病的风险,低盐低脂功能性肉制品的开发受到重视。本文主要综述了低盐低脂功能性肉制品的开发研究进展,并指出功能性肉制品发展中存在的局限性,以期为功能性肉制品的研究和开发提供理论参考。      

肉及肉制品碘含量检测新方法的验证

对即将实施的碘含量检测新方法进行验证分析,通过选取具有代表性的样本进行实验,研究了新方法对肉及肉制品检测结果准确性和精密度的影响.结果表明,与现有检测方法相比,新检测方法的准确性更好、精密度更高,更加适用于肉及肉制品中碘含量的测定.     

New strategies for the development of innovative fermented meat products: a review regarding the incorporation of probiotics and dietary fibers

Over the last decades, consumer´s preference for and their attention to food products presented as healthy and with favorable nutritional information has been significantly increased. In this line, both the addition of dietary fiber and the incorporation of probiotic strains in the elaboration of fermented meat products has been established as a useful tool for the development of healthy products. Thus, the aim of this review is to present an overview of the studies involving fermented meat products with added dietary fiber and probiotic microorganisms, and also, to discuss about some of the challenges regarding the reformulation of this innovative product category.     

提高肉制品保水性方法的研究进展

肉制品的保水性是衡量肉制品品质和经济价值的重要指标之一,如何提高肉制品的保水性具有重要意义。本文对影响肉制品保水性的因素和提高肉制品保水性的方法进行了综述,希望为肉制品的生产加工提供参考。      

食品添加剂在改善肉制品色泽中的应用

概述了食品添加刺在改善肉制品色泽应用的原理与方法.     

环介导等温扩增技术快速检测肉及肉制品中的牛源性成分

本文以牛源性线粒体细胞色素B(cyt B)基因为模板,采用软件Primer Explorer Version 4设计并筛选出LAMP特异性扩增引物,通过反应体系优化建立了肉及肉制品中牛源性成分的环介导等温扩增技术检测方法,对该方法的灵敏度、特异性以及稳定性进行了验证,同时采用国标方法和商品化检测试剂盒对市售的12种牛肉及牛肉制品进行对比检测。结果表明该检测方法具有高灵敏度、高特异性和高稳定性,适用于实际的生鲜肉制品及加工肉制品中牛源性成分的鉴定。      

膜芯片用于肉制品中动物源性成分检测的研究

目的研究膜芯片技术检测肉类及其制品中动物源性成分,验证该技术的准确性及可行性。方法利用膜芯片检测肉制品中的动物源性成分,对样品中猪、牦牛、驴及羊源性成分的灵敏度、检出限进行相关实验,并就实际样品的检测与国家标准进行比较。结果该技术用于动物源性成分检测特异性良好,4种动物源性检测的灵敏度为0.1 ng,检出限为0.1%(w:w),适用的样品种类较广,实际样品的检测结果与国家标准荧光PCR法一致。结论该技术可作为快速筛选的方法,为肉制品的动物源性成分鉴定和掺假检测提供理论依据。     

An assessment of commercially available reagents for an enzyme-linked immunosorbent assay (ELISA) of soya protein in meat products

An enzyme-linked immunosorbent assay (ELISA) procedure based on a limited supply of specially prepared experimental immunoreagents and designed to measure levels of soya protein in raw or processed mixed meat products has already been published. This report describes a modified method with commercially available immunoreagents suitable for routine use; its response to a range of commercial soya ingredients has been checked using a particular soya protein isolate (Unisol, Unimills BV) as arbitrary standard. Individual soya components and possible cross-reacting food materials have also been investigated. Both the original and modified methods have been applied to a set of model beefburgers containing known levels of Unisol. There was good agreement between observed and calculated levels in raw and heat-set beefburgers; sterilised samples gave a decreased but linear response. The procedure is recommended for the examination of meat products in non-specialised laboratories.     

植物蛋白基肉制品的营养安全性分析

随着全球人口不断增长、环境压力、动物福利及可持续性发展等问题的出现,传统养殖业及肉制品产业正面临着日益严峻的挑战.人口不断增长,消费者对蛋白质需求不断增加,植物蛋白基肉制品作为一种肉类替代品可以满足消费者需求并解决粮食紧缺问题.肉类替代品也因其资源天然性、绿色制造、可持续发展等方面的优势而受到广泛关注.食品安全问题是社...