GC法同时测定麝香跌打风湿膏中的3种挥发性成分

目的 采用顶空气相色谱法同时测定麝香跌打风湿膏中3种挥发性成分的含量.方法 用氯仿超声溶解麝香跌打风湿膏后,以水为顶空溶剂,在80℃下平衡40 min,采用TM-WAX(50 m×0.53 mm,0.5 μm)毛细管柱、FID检测器进行气相色谱检测,程序升温,初始温度100℃,以4℃·min-1升至150 ℃,保持2 min,进样口温度200℃,检测器温度210℃.结果 樟脑、薄荷脑、水杨酸甲酯分别在10.0～160、5.17 ～82.7、7.42 ～119 μg·mL-1时线性关系良好(r樟脑=0.9994,r薄荷脑=0.9992,r水杨酸甲酯=0.9993),3者的平均回收率均在93.3％ ～101％,RSD均小于3.6％.结论 所用方法简单、快速、灵敏度高,适用于同时测定麝香跌打风湿膏中挥发性成分樟脑、薄荷脑、水杨酸甲酯的含量.     

气相色谱法测定亚微乳凝胶剂中樟脑、薄荷脑、水杨酸甲酯的含量

目的:建立气相色谱法测定亚微乳凝胶剂中樟脑、薄荷脑、水杨酸甲酯含量的方法。方法:气相色谱柱为 SE-54柱(30m×0.32 mm,0.25μm);环己酮为内标。采用 FID 检测器,进样口温度230℃,检测器温度250℃,采用程序升温,起始温度90℃,保持5 min,以10℃·min~(-1)升到150℃,保持2 min。结果:樟脑、薄荷脑、水杨酸甲酯线性范围分别为0.18～1.60 mg·mL~(-1)(r=0.9999),0.17～1.55 mg·mL~(-1)(r=0.9998),0.40～3.59 mg·mL~(-1)(r=0.9999);平均回收率分别为99.5%,99.2%,99.1%;RSD 均小于2%(n=9)。结论:本法方便,准确,重现性好,可作为本制剂的质量控制方法。     

气相色谱法同时测定无极膏中5个挥发性有效成分的含量

目的:建立同时测定无极膏中合成樟脑、薄荷脑、水杨酸甲酯、冰片和麝香草酚5种主要成分含量的分析方法。方法:采用气相色谱法,色谱柱为聚乙二醇(PEG)-20M毛细管色谱柱(30 m×0.32 mm×0.25μm),检测器为FID检测器,进样口温度为250℃,检测器温度为250℃。升温程序为初始温度150℃,保持5 min;10℃.min-1升温至200℃,保持8 min;再以20℃.min-1升温至240℃,保持25 min,分流比为10∶1。结果:合成樟脑、薄荷脑、水杨酸甲酯、冰片和麝香草酚浓度分别在1.4934～7.4672 mg.mL-1(r=0.9999),0.9304～4.6520 mg.mL-1(r=0.9999),0.9075～4.5376 mg.mL-1(r=0.9999),0.1387～0.6936 mg.mL-1(r=0.9999),0.0716～0.3580 mg.mL-1(r=0.9999)质量浓度范围内线性关系良好。平均回收率(n=9)分别为98.8%,99.4%,98.6%,99.6%,99.7%。结论:该方法灵敏、快速简便、准确,可有效地控制无极膏的质量。     

气相色谱法测定神农镇痛膏中樟脑和水杨酸甲酯的含量

目的:建立气相色谱(GC)法同时测定神农镇痛膏中樟脑和水杨酸甲酯的含量。方法:色谱柱为 RTX-WAX 石英毛细管柱(0.53mm×15m),检测器为 FID 检测器,检测器温度为220℃,进样口温度:200℃,柱温为140℃。结果:樟脑和水杨酸甲酯的线性范围分别在0.22～5.43μg(r=0.9999)和0.10～10.04μg(r=0.9999),回收率分别为97.1%(RSD=1.4%)和98.6%(RSD=2.5%)。结论:该法快速、准确、可靠,可用于神农镇痛膏的质量控制。     

气相色谱法同时测定拜尼多中4种有效成分含量

目的:建立气相色谱法同时测定拜尼多(康松无极膏)中薄荷脑、樟脑、水杨酸甲酯及麝香草酚的含量。方法:采用气相色谱法,色谱柱为INNOWAX石英毛细管柱(0.32 mm×30 m,0.25μm),采用程序升温法,检测器温度为250℃。结果:薄荷脑、樟脑、水杨酸甲酯及麝香草酚的回归方程分别为Y=190375.67 9507.62X(r=0.9999),Y=80436.70 9030.67X(r=0.9995),Y=73630.95 6474.46X(r=0.9999),Y=8653.41 432.18X(r=0.9998);线性范围分别为108.2—1406.6,422.0—2100.0,100.7—906.3,8.3—75.5μg·mL~(-1);回收率分别为102.6%-105.5%,109.5%-119.0%,104.0%-107.5%,100.6%-104.3%。结论:该法快速、准确、可靠,适用于康松无极膏的含量测定。     

GC法测定痛肿灵酊中樟脑、薄荷脑、异龙脑、龙脑的含量

目的:建立气相色谱法测定痛肿灵酊中樟脑、薄荷脑、异龙脑、龙脑含量的方法.方法:色谱柱:Phenomenex ZB-WAX 石英毛细管柱(30 m×0.32 mm,0.25μm),火焰离子化检测器温度:280℃,进样口温度:250℃,柱温:130℃.结果:樟脑在0.110～5.492 μg范围内线性关系良好(r=0.999 5),平均回收率为99.04％,RSD=0.28％(n=9);薄荷脑在0.120 ～6.024 μg范围内线性关系良好(r =0.9996),平均回收率为99.16％,RSD =0.50％ (n =9);异龙脑在0.028 ～1.402 μg范围内线性关系良好(r =0.9991),平均回收率为99.03％,RSD =0.40％ (n =9),龙脑在0.034 ～ 1.700 μg范围内线性关系良好(r=0.9998),平均回收率为99.27％,RSD =0.41％ (n =9).阴性样品无干扰.结论:该法操作简便,结果准确可靠,可作为痛肿灵酊的质量控制方法.     

气相色谱法测定无极膏中5种成分的含量

目的建立一种用气相色谱法测定无极膏中5种成分含量的方法.方法采用HP-INNOWAX Polyethylene Glycol(30.0 m×250 μm×0.25 μm)毛细管色谱柱,柱温采用程序升温,进样口温度200℃,FID检测器,检测器温度250℃.结果样品中5种成分完全分离且线性关系良好,樟脑、薄荷脑、龙脑、水杨酸甲酯、麝香草酚的加样回收率分别为97.3%、98.9%、98.3%、101.3%和100.8%(n=9).结论该法简便快速,结果准确,可以有效地控制质量.     

气相色谱法测定风油精中5种成分的含量

目的研究提高风油精中薄荷脑、桉油精、樟脑、丁香酚、水杨酸甲酯5种成分的含量测定方法。方法色谱柱：Agilent INNOWAX石英毛细管柱（30m×0.32mm×0.25μm）;载气为氮气;柱温采用程序升温：初始温度85℃,保持4min,以每分钟30℃的速率升至115℃,保持0min,以每分钟70℃的速率升至200℃,保持4min,以每分钟100℃的速率升至250℃,保持3min;检测器：FID;采用内标法测定。结果薄荷脑、桉油精、樟脑、水杨酸甲酯、丁香酚线性范围为0.34～17.22mg.mL-1（r=1.0000）、0.05～2.26mg.mL-1（r=1.0000）,0.04～1.93mg.mL-1（r=0.9999）,0.34～17.16mg.mL-1（r=1.0000）、0.04～1.80mg.mL-1（r=1.0000）,平均回收率为96.17%、96.69%、101.14%、96.86%、98.02%。结论该方法经济、简单、准确且重现性好,可作为风油精的含量测定质量控制标准。     

GC法测定精制狗皮膏中四种成分的含量

目的:采用气相色谱法测定精制狗皮膏中樟脑、薄荷脑、龙脑、水杨酸甲酯的含量。方法:气相色谱法,弹性石英毛细管柱(30m×0.32mm×0.25μm),聚乙二醇(PEG)-20M为固定液,FID检测器。结果:所测樟脑、薄荷脑、龙脑、水杨酸甲酯之间具有良好的分离效果,樟脑的经性范围为160～960μg/ml,平均回收率101.1%,RSD为2.74%;薄荷脑的线性范围为160～960μg/ml,平均回收率102.2%,RSD为2.42%;龙脑的线性范围为40～240μg/ml,平均回收率101.4%,RSD为2.15%;水杨酸甲酯的线性范围时80～480μg/ml,平均回收率102.0%,RSD为2.54%。结论:本方法简便、快速、结果准确,可用于精制狗皮膏的质量控制。     

毛细管气相色谱法同时测定少林风湿跌打膏中薄荷脑、水杨酸甲酯和冰片的含量

目的:建立毛细管气相色谱法同时测定少林风湿跌打膏中薄荷脑、水杨酸甲酯和冰片的含量。方法:采用水蒸气蒸馏法提取制备样品溶液。HP-INNOWax Polyethylene Glycol(30 m×0.35 mm×0.25μm)石英毛细管色谱柱,柱温110℃,进样口温度200℃,氢火焰离子化检测器(FID),分流进样,分流比10.0∶1,载气为氮气,流速1.0 mL.min-1,进样量1μL。结果:薄荷脑在0.423~2.642 mg.mL-1范围内与峰面积呈良好线性关系(r=0.9999),水杨酸甲酯在0.405~2.532 mg.mL-1范围内与峰面积呈良好线性关系(r=0.9999),冰片在0.412~2.575 mg.mL-1范围内与峰面积呈良好线性关系(r=0.9999);平均加样回收率薄荷脑为102.2%(RSD=2.0%),水杨酸甲酯为102.1%(RSD=2.0%),冰片为102.5%,(RSD=2.0%)。结论:本方法简便、准确、重现性好,可用于少林风湿跌打膏制剂及中间体的质量控制。     

Safety assessment of poloxamers 101, 105, 108, 122, 123, 124, 181, 182, 183, 184, 185, 188, 212, 215, 217, 231, 234, 235, 237, 238, 282, 284, 288, 331, 333, 334, 335, 338, 401, 402, 403, and 407, poloxamer 105 benzoate, and poloxamer 182 dibenzoate as used in cosmetics

Poloxamers are polyoxyethlyene, polyoxypropylene block polymers. The impurities of commercial grade Poloxamer 188, as an example, include low-molecular-weight substances (aldehydes and both formic and acetic acids), as well as 1,4-dioxane and residual ethylene oxide and propylene oxide. Most Poloxamers function in cosmetics as surfactants, emulsifying agents, cleansing agents, and/or solubilizing agents, and are used in 141 cosmetic products at concentrations from 0.005% to 20%. Poloxamers injected intravenously in animals are rapidly excreted in the urine, with some accumulation in lung, liver, brain, and kidney tissue. In humans, the plasma concentration of Poloxamer 188 (given intravenously) reached a maximum at 1 h, then reached a steady state. Poloxamers generally were ineffective in wound healing, but were effective in reducing postsurgical adhesions in several test systems. Poloxamers can cause hypercholesterolemia and hypertriglyceridemia in animals, but overall, they are relatively nontoxic to animals, with LD(50) values reported from 5 to 34.6 g/kg. Short-term intravenous doses up to 4 g/kg of Poloxamer 108 produced no change in body weights, but did result in diffuse hepatocellular vacuolization, renal tubular dilation in kidneys, and dose-dependent vacuolization of epithelial cells in the proximal convoluted tubules. A short-term inhalation toxicity study of Poloxamer 101 at 97 mg/m(3) identified slight alveolitis after 2 weeks of exposure, which subsided in the 2-week postexposure observation period. A short-term dermal toxicity study of Poloxamer 184 in rabbits at doses up to 1000 mg/kg produced slight erythema and slight intradermal inflammatory response on histological examination, but no dose-dependent body weight, hematology, blood chemistry, or organ weight changes. A 6-month feeding study in rats and dogs of Poloxamer 188 at exposures up to 5% in the diet produced no adverse effects. Likewise, Poloxamer 331 (tested up to 0.5 g/kg day(-1)), Poloxamer 235 (tested up to 1.0 g/kg day(-1)), and Poloxamer 338 (at 0.2 or 1.0 g/kg day(-1)) produced no adverse effects in dogs. Poloxamer 338 (at 5.0 g/kg day(-1)) produced slight transient diarrhea in dogs. Poloxamer 188 at levels up to 7.5% in diet given to rats in a 2-year feeding study produced diarrhea at 5% and 7.5% levels, a small decrease in growth at the 7.5% level, but no change in survival. Doses up to 0.5 mg/kg day(-1) for 2 years using rats produced yellow discoloration of the serum, high serum alkaline phosphatase activity, and elevated serum glutamicpyruvic transaminase and glutamic-oxalacetic transaminase activities. Poloxamers are minimal ocular irritants, but are not dermal irritants or sensitizers in animals. Data on reproductive and developmental toxicity of Poloxamers were not found. An Ames test did not identify any mutagenic activity of Poloxamer 407, with or without metabolic activation. Several studies have suggested anticarcinogenic effects of Poloxamers. Poloxamers appear to increase the sensitivity to anticancer drugs of multidrug-resistant cancer cells. In clinical testing, Poloxamer 188 increased the hydration of feces when used in combination with a bulk laxative treatment. Compared to controls, one study of angioplasty patients receiving Poloxamer 188 found a reduced myocardial infarct size and a reduced incidence of reinfarction, with no evidence of toxicity, but two other studies found no effect. Poloxamer 188 given to patients suffering from sickle cell disease had decreased pain and decreased hospitilization, compared to controls. Clinical tests of dermal irritation and sensitization were uniformly negative. The Cosmetic Ingredient Review (CIR) Expert Panel stressed that the cosmetic industry should continue to use the necessary purification procedures to keep the levels below established limits for ethylene oxide, propylene oxide, and 1,4-dioxane. The Panel did note the absence of reproductive and developmental toxicity data, but, based on molecular weight and solubility, there should be little skin penetration and any penetration of the skin should be slow. Also, the available data demonstrate that Poloxamers that are introduced into the body via routes other than dermal exposure have a rapid clearance from the body, suggesting that there would be no risk of reproductive and/or developmental toxicity. Overall, the available data do not suggest any concern about carcinogenesis. Although there are gaps in knowledge about product use, the overall information available on the types of products in which these ingredients are used, and at what concentration, indicates a pattern of use. Based on these safety test data and the information that the manufacturing process can be controlled to limit unwanted impurities, the Panel concluded that these Poloxamers are safe as used.     

HPLC法测定抗妇炎胶囊中木兰花碱、黄柏碱、药根碱、巴马汀、小檗碱、槐果碱、苦参碱、氧化槐果碱、槐定碱和氧化苦参碱

目的 采用高效液相色谱（HPLC）法同时测定抗妇炎胶囊中木兰花碱、黄柏碱、药根碱、巴马汀、小檗碱、槐果碱、苦参碱、氧化槐果碱、槐定碱和氧化苦参碱10种活性成分。方法 采用InerSustain AQ-C₁₈色谱柱（250 mm×4.6 mm,5 μm）,流动相A：乙腈–无水乙醇（80∶20）,流动相B：0.1%磷酸溶液,梯度洗脱,检测波长220 nm,体积流量1.0 mL/min,柱温30℃,进样量10 μL。结果 木兰花碱、黄柏碱、药根碱、巴马汀、小檗碱、槐果碱、苦参碱、氧化槐果碱、槐定碱和氧化苦参碱分别在2.69～134.50、1.95～97.50、0.63～31.50、0.86～43.00、11.95～597.50、0.59～29.50、6.08～304.00、4.85～242.50、1.66～83.00、19.79～989.50 μg/mL线性关系良好（r≥0.999 3）;平均回收率分别为99.11%、98.23%、96.95%、97.78%、100.02%、97.21%、99.66%、99.52%、98.81%、100.08%,RSD值分别为1.04%、1.23%、1.37%、1.65%、0.70%、1.28%、0.65%、0.81%、1.11%、0.63%。结论 建立的HPLC法可用于抗妇炎胶囊中10种活性成分的测定,作为抗妇炎胶囊质量控制方法。     

Arformoterol: (R,R)-eformoterol, (R,R)-formoterol, arformoterol tartrate, eformoterol-sepracor, formoterol-sepracor, R,R-eformoterol, R,R-formoterol

Sepracor in the US is developing arformoterol [R,R-formoterol], a single isomer form of the beta(2)-adrenoceptor agonist formoterol [eformoterol]. This isomer contains two chiral centres and is being developed as an inhaled preparation for the treatment of respiratory disorders. Sepracor believes that arformoterol has the potential to be a once-daily therapy with a rapid onset of action and a duration of effect exceeding 12 hours. In 1995, Sepracor acquired New England Pharmaceuticals, a manufacturer of metered-dose and dry powder inhalers, for the purpose of preparing formulations of levosalbutamol and arformoterol. Phase II dose-ranging clinical studies of arformoterol as a longer-acting, complementary bronchodilator were completed successfully in the fourth quarter of 2000. Phase III trials of arformoterol began in September 2001. The indications for the drug appeared to be asthma and chronic obstructive pulmonary disease (COPD). However, an update of the pharmaceutical product information on the Sepracor website in September 2003 listed COPD maintenance therapy as the only indication for arformoterol. In October 2002, Sepracor stated that two pivotal phase III studies were ongoing in 1600 patients. Sepracor estimates that its NDA submission for arformoterol, which is projected for the first half of 2004, will include approximately 3000 adult subjects. Sepracor stated in July 2003 that it had completed more than 100 preclinical studies and initiated or completed 15 clinical studies for arformoterol inhalation solution for the treatment of bronchospasm in patients with COPD. In addition, Sepracor stated that the two pivotal phase III studies in 1600 patients were still progressing. In 1995, European patents were granted to Sepracor for the use of arformoterol in the treatment of asthma, and the US patent application was pending.     

欧洲刺柏（Juniper）

活性成分与药理作用欧洲刺柏药用部位是其浆果，具有促水排泄、防腐、抗胃肠胀气和抗风湿作用，还可改善胃功能。用作促水排泄药可增加尿量（水丢失），但不增加钠排泄。成分萜品烯-4-醇可增加肾小球滤过率，但刺激肾。欧洲刺柏浆果对单纯疱疹病毒体外显示抗病毒活性，并具抗真菌活性。动物实验显示，欧洲刺柏浆果提取物具有堕胎、抗生育、抗炎、抗胚胎植入、降血压、升血压和降血糖作用。欧洲刺柏浆果油具有兴奋子宫的活性，以及利尿、胃肠道抗菌和刺激作用，该油对平滑肌有阻止解痉作用。     

Oral Presentations (LDD, LPES, LNM, LPAT, LNT, LPPT, LPM)

Probiotic microorganisms have been shown to provide specific health benefits when consumed as food supplements or as food components. The main problem of such products is the poor survival of the probiotic bacteria in the low pH of gastric fluid. However the use of synthetic excipients for enteric coating to prevent the exposure of microorganisms to gastric fluid is limited in food supplementary industry. Therefore the aim of this study was to develop an enteric coating formulation containing shellac as a natural polymer. Shellac possesses good resistance to gastric juice; the major disadvantage of this polymer is its low solubility in the intestinal fluid [1, 2]. Thus films containing different ratios of shellac and water-soluble polymers (sodium alginate, hydroxypropyl methylcellulose (HPMC) and polyvinylpyrrolidon (PVP)) or plasticizers (glycerol and glyceryl triacetate (GTA)) were prepared in order to analyse the films’ melting temperatures (T_m), the changes in enthalpy (ΔH), their capability of taking up water, and their solubility in different media. The release characteristics of the films were studied by loading pellets with Enterococcus faecium M74 and coating them with formulations containing different amounts of shellac and polymer or plasticized shellac. Using dissolution tests, performed according to USP XXXI paddle method, the resistance of the coatings to simulated gastric fluid (SGF, pH 1.2) and the release of cells in simulated intestinal fluid (SIF, pH 6.8) was investigated.The trials showed that an increasing amount of plasticizer results in a decrease of T_m and ΔH of the films whereat glycerol had a superior plasticization effect to GTA. The compatibility of films made of water-soluble polymers and shellac was also concentration dependent. HPMC and PVP showed superior compatibility with shellac compared to sodium alginate, since films containing shellac and more than 10% [w/w] sodium alginate tended to separate into two phases. In the end five formulations containing shellac and either 5% [w/w] glycerol, 10% [w/w] PVP, 20% [w/w] PVP, 10% [w/w] HPMC, or 5% [w/w] sodium alginate emerged as feasible for enteric coating purposes.