变形链球菌和放线菌与根面龋关系的研究

目的 分析变形链球菌和放线菌与根面龋的关系。方法 用选择性培养基ＭＳ和ＢＹＳ对３０例根面龋中的变形链球菌、放线菌定量研究。结果 放线菌的检出率１００％,变形链球菌的检出率９３．３３％,放线菌在数量上（ＣＦＵ）占绝对优势,与变形链球菌数量相比有高度显著性差异,Ｐ〈０．０１。结论 支持放线菌是根面龋优势菌的观点。     

变形链球菌和粘性放线菌致牙根部龋体外实验研究

牙根龋是老年口腔常见病之一,它同牙冠部釉质龋一祥也是在菌斑作用下形成的。有报道表明,牙根暴露以后,口腔内多种细菌在根部牙骨质上定居并形成菌斑,其中主要是粘性放线菌(简称粘放)和变形链球菌(简称变链)。这两种细菌都与牙根龋形成有关。在牙根龋发生中,细菌产     

牙周基础治疗前后根面变形链球菌的变化

目的:研究牙周基础治疗前后患者根面变形链球菌的改变情况,并与根而龋患者根面变形链球菌的检出情况相比较,初步探讨致龋微生物变形链球菌与牙周基础治疗后根面龋易感性的相关性.方法:采集20例牙周(实验组)基础治疗前、治疗后1周、1、2、3月根面菌斑,及20例根面龋患者(对照组)根面龋处菌斑.在选择性培养基上厌氧培养分离变形链球菌,并通过生化反应鉴定细菌种类.结果:变形链球菌检出分析:实验组在牙周基础治疗后1周、1、2月细菌计数与对照组比较差异无显著性,而实验组在基础治疗前、治疗后3月细菌计数低,分别与对照组比较差异有统计学意义(P＜0.05).结论:牙周基础治疗根面暴露可能增加根面龋发生的风险.在牙周基础治疗后3个月内要采取防护措施.     

老年人根面龋菌斑内氏放线菌临床株的分离与鉴定

目的：分析内氏放线菌在根面龋老年患者与无根面龋老年人中的分布状况,初步探讨内氏放线菌与根面龋的相关关系。方法：根据纳入标准从临床就诊老年人中随机选择老年根面龋患者及无根面龋老年人各20名。根面龋患者每位受检者选择2个菌斑取样位点：A点为暴露的无龋根面;B点为根面龋龋坏部位。无根面龋老年人每位受检者选择1个菌斑取样位点（C点）：暴露的根面。刮取根面菌斑,螺旋接种仪接种,BHI培养、分离并生化鉴定内氏放线菌。结果：老年根面龋患者的龋坏根面、无龋根面以及无根龋老年人的暴露根面,内氏放线菌检出率无明显差异（P〉0．05）,内氏放线菌检出量有差异（P〈0．05）。数目由高到低依次为：无根面龋老年人牙根面,根龋患者无龋根面,根龋患者龋坏根面。结论：内氏放线菌在老年人根面有较高的检出率,与根面龋密切相关。而检出量有差异,根面龋损部位内氏放线菌检出量低于根面龋患者无龋根面以及无根面龋老年人牙根面。     

根面龋病灶微生物学研究

作者对13例根面龋患者病灶微生态系中的变形链球菌、乳酸杆菌、粘性放线菌进行定量研究。结果显示,变形链球菌在数量上占绝对优势,乳酸杆菌也较多,而粘性放线菌仅在1例中检出。故支持变形链球菌是根面龋和冠龋共同病原菌的观点。     

儿童唾液变形链球菌与患龋状况关系的研究

目的：探讨唾液变形链球菌与儿童龋病的关系。方法：通过口腔健康检查,问卷调查等全面了解并分析350名3～7岁在园儿童龋患情况及影响乳牙龋病发生的危险因素。采用直接接种轻唾培养基法检测唾液中变形链球菌浓度,探讨其与患龋情况的关系。结果：350名被检儿童中263名患龋,总患龋率75.14%,龋均4.73,龋面均11.11。单因素分析显示年龄、牛奶或饮用水加糖、吃甜食及糖果糕点次数、父亲刷牙情况及月家庭经济收入与儿童的龋患程度相关（P〈0.05）。多因素回归分析显示年龄、牛奶或饮水加糖、每日进食糖果糕点次数、父亲刷牙情况是乳牙龋的重要影响因素。350例儿童唾液变形链球菌菌落数中位数为1.61×105 CFU/mL,无龋（dmfs=0）、低龋（1≤dmft〈6）、高龋（dmfs≥6）儿童间变形链球菌浓度差异有统计学意义（P〈0.05）。其中184名早期入园的儿童通过为期一年的追踪观察显示唾液变形链球菌浓度与新增dmfs呈正相关关系。结论：儿童唾液变形链球菌浓度与龋患严重程度以及龋活跃性相关。     

变形链球菌和远缘链球菌致龋性的研究近况

变形链球菌和远缘链球菌是人类牙齿的主要致龋菌。近年研究表明两种细菌的生物学特性存在一定差异，远缘链球菌与龋病的活跃性密切相关，本文对两种细菌的流行病学、粘附机理、多的合成产酸酸耐酸进行了综述，为今后龋病病因学研究，抗龋疫的制备提供参考。     

白色念珠菌、黏性放线菌与根面龋相关性的研究进展

根面龋是老年人龋病的主要表现形式.由于牙根部牙骨质及牙本质中相对更高的有机物组成和其表面特殊的微环境,根面龋的发生过程相对复杂.白色念珠菌和黏性放线菌作为口腔常驻共生菌群,具有产酸、耐酸和酵解有机物等性能,在根面龋发生发展中扮演着重要角色.本文就白色念珠菌、黏性放线菌与根面龋的研究进展作一综述,以期从真菌-细菌控制的角...     

无龋根面和牙骨质龋菌斑菌丛的研究

本实验选择门诊患者28人,分为无龋恨面组和牙骨质龋组进行了细菌学研究。结果提示:轻链菌、粘放菌和内氏放线菌是参与菌斑组成的基本成员;变链菌在牙骨质龋组中显著多于正常无龋根面组达10倍以上,在统计学上有极显著差异,提示变链菌为根龋的主要致病菌;而韦荣氏菌是基础牙菌斑微生物群落成员之一。     

老年人根面龋

根面龋是老年人常见的牙体病之一。其发病情况、临床表现及治疗等方面具有一定的特殊性,与冠部龋不完全相同。治疗和保存根面龋牙,对恢复老年人的咀嚼功能具有重要的意义。我们于1986年3月至1989年5月,对60岁以上老年人86例172颗根面龋牙进行治疗,总结如下。     

Proportions of Streptococcus mutans, lactobacilli and Actinomyces spp in root surface plaque

Abstract – The incidence of Streptococcus mutans, lactobacilli and Actinomyces was examined in root surface dental plaque from 24 subjects with root surface caries and in 24 subjects with exposed root surfaces as a consequence of periodontitis but without root surface caries. Plaque was collected from the most posterior teeth in each quadrant: in the caries group from carious lesions and in the periodontitis group from buccal root surfaces. There were significantly higher proportions of S. mutans and lactobacilli in dental plaque samples taken from subjects suffering from root surface caries than in samples from subjects without root surface caries. The level of the group A. viscosus/naeslundii in the periodontitis group was higher than that found in the root surface caries group, but the difference was not statistically significant.     

Streptococcus mutans and dental caries in urban and rural schoolchildren in Thailand

Two groups of Thai schoolchildren aged 11-13 yr were examined for dental caries, dental fluorosis and Streptococcus mutans. One hundred children lived in an urban district, Bangkok, and 71 children in a rural district, Petchaboon. Saliva samples were analyzed for S. mutans by the spatula method. The fluoride content of the drinking waters was also determined. The prevalence of caries, diagnosed according to WHO, was 89% in Bangkok and 18% in the rural district. Mean DMFT was 3.46 and 0.38 respectively. S. mutans was found in 98% of the urban children and in 82% of the rural. The differences in distribution between S. mutans and DMFT classes were statistically significant for the total sample. Mild fluorosis was present in some of the rural children.     

Prevalence of dental caries and Streptococcus mutans in Meo of Northern Thailand

The prevalence of dental caries was studied in 505 Meo, a minority of Northern Thailand, using the DMFT index. Plaque material from all patients was examined qualitatively for S. mutans. The DMFT (dmft) index score ranged between 1.69 and 7.06 with a mean of 2.17 +/- 3.24 for men and 3.45 +/- 3.72 for women. There was a statistical difference in caries prevalence between males and females (P less than 0.05). There was no statistical correlation between miang chewing and caries prevalence. Fluoride concentration of water samples was between 0.043 and 0.077 ppm F-. Of 505 plaque samples, 13.3% were positive for S. mutans, mostly of biotype I. Compared to other Northern Thai hill tribes and the Thai rural population, the Meo appear to have a higher dental caries prevalence rate.     

Streptococcus mutans establishment and dental caries experience in children from 2 to 4 years old

Abstract – 39 children were studied longitudinally at the age of 2, 3, and 4 yr for the colonization of S. mutans in plaque and saliva and for caries experience. S. mutans was found in 38% of the children, and the predominant serotype group was c/e/f. A total of 16 children got caries before the age of 4. Children who harbored S. mutans in their plaque at the age of 2, appeared to be the most caries-active individuals. Their caries index values (number of decayed, missed and filled surfaces, dmfs = 10.6±5.3) at the age of 4 differed significantly from the values of children who harbored S. mutans later (dmfs = 3.4±1.8,P<0.005) or remained free from S. mutans infection (dmfs = 0.3±1.1, P<0.0003). It was thus concluded that the early establishment of S. mutans in the plaque of primary incisors indicated early and extensive caries attack in young primary dentition.     

Salivary Streptococcus mutans and caries prevalence in Brazilian schoolchildren

The aim of the present study was to determine the levels of salivary Streptococcus mutans in Brazilian schoolchildren, and its possible correlation with caries prevalence. A positive correlation between different levels of S. mutans and caries prevalence was observed, individuals with high levels of S. mutans (greater than 100 colonies) had a DMFS significantly higher than those with low or no detectable S. mutans (0, 1-20, 21-40 colonies).     

变异链球菌噬菌体在龋病防治中的研究进展

变异链球菌作为主要致龋病原菌之一,其在牙菌斑生物膜中的过度增殖,是龋病发生发展的重要原因。细菌病毒——噬菌体可特异性感染细菌并有效降解细菌生物膜,因此变异链球菌噬菌体可能用于防治龋病。基于噬菌体的疗法已在多个领域进行临床应用,但变异链球菌噬菌体在龋病中的应用仍处于探索阶段。本文对变异链球菌噬菌体在龋病防治方面的应用进行综述,旨在为龋病的临床预防工作提供新的思路。文献复习结果表明,活噬菌体制剂具有特异性强、亲和力高以及安全性良好的优点,但由于其结构较不稳定,可以通过冻干、喷雾干燥、添加稳定性增强剂等方式,或将噬菌体并入软膏、可生物降解聚合物基质或微粒中加工成较稳定的制剂,在一定程度上提高稳定性;噬菌体产生的裂解酶可以消化细菌细胞壁,从而释放组装好的噬菌体颗粒,其在裂解生物膜中也同样表现优异;通过噬菌体展示技术筛选出针对致龋病原体的抗原结合片段库,利用抗原结合片段的被动免疫也可达到防治龋病的目的。然而,噬菌体的宿主范围狭窄,因此在临床龋病防治工作中可以通过噬菌体联合传统治疗或其他药物使用,或使用多种噬菌体的鸡尾酒治疗。     

树脂充填前后唾液中变异链球菌的变化

目的 观察使用树脂进行龋齿治疗前后唾液中变异链球菌数量的变化.方法 招募志愿者20名,对其口腔内所有龋齿去净龋坏组织后使用不含氟树脂材料进行充填,分别收集充填前及充填后第1、2、3、4周的非刺激性唾液样本,使用轻唾-杆菌肽琼脂培养基厌氧培养48 h后检测变异链球菌数量,比较其在龋齿充填治疗前后的变化.结果 龋齿行树脂材料充填后1周唾液中变异链球菌数量较充填前下降明显(P＜0.05),充填后2、3、4周较充填前无明显变化(P＞0.05).结论 对患龋个体进行龋齿备洞后的树脂充填能在短期内降低唾液中变异链球菌的含量,但随时间推移,唾液中变异链球菌数量会逐渐恢复到治疗前的状态.单纯依靠龋齿充填治疗并不能改变唾液中变异链球菌的数量.     

变形链球菌GbpA的GBD基因疫苗动物免疫防龋研究

目的：研究变形链球菌GbpA的GBD基因疫苗免疫SD大鼠时,其所诱导的血清和唾液特异性免疫反应及防龋效果。方法：实验分2组,实验组用纯化的变形链球菌GbpA的GBD真核表达质粒pcDNA3．1-GBD颌下腺周围皮下免疫SD大鼠,其中实验1组免疫后间隔一定的时间收集大鼠的血清及唾液,间接ELISA检测血清及唾液中特异性抗GBD抗体,实验2组免疫同时喂致龋饲料—Keyes改良的高糖Diet 2000,并于第一次注射20d时连续3d大鼠口腔中接种S．mutans Ingbritt,在接种S．mutans后第77 d处死大鼠,收集大鼠颌骨标本用于龋齿记分分析。对照组2只大鼠颌下腺周皮下注射1×PBS(pH7．4)。结果：用纯化的pcDNA3．1-GBD基因疫苗免疫大鼠,血清中IgG及唾液中IgA明显升高,大鼠高糖饮食及口腔接种S．mutans Ingbritt,实验组的龋患率明显低于对照组,说明GBD基因疫苗具有免疫防龋作用。结论：变形链球菌GbpA的GBD基因疫苗具有免疫原性,是一种有效的免疫防龋疫苗。     

口腔龋坏组织中变异链球菌与乳酸杆菌的作用研究

目的分析人龋坏组织中变异链球菌与乳酸杆菌在不同分组中的培养计数及不同细菌主导的病例数,研究变异链球菌和乳酸杆菌在致龋过程中的协同作用。方法选取甘肃省靖煤公司总医院口腔科110例龋病患者,按照不同龋坏程度、不同年龄、不同龋坏性质分组采集龋坏组织,在选择培养基上培养,计数菌落数量。统计2种细菌在不同分组中的检出率、细菌计数平均数及不同致龋菌病例数,研究二者的致龋作用。结果变异链球菌和乳酸杆菌的细菌计数平均数随着龋坏程度增加而增高（P<0.05）,在老年组患者中变异链球菌和乳酸杆菌数量增高（P<0.05）,在龋病活动期增高（P<0.05）。随着龋坏程度的增加,变异链球菌和乳酸杆菌共同致龋病例数增高（P<0.05）,在老年人龋坏中共同致龋病例数增高（P<0.05）,变异链球菌和乳酸杆菌共同致龋作用与龋病的静止与活动无相关性。结论变异链球菌和乳酸杆菌随着龋坏程度的增加,共同致龋作用增强;老年人龋齿中,二者的协同作用占主导作用;在龋病活动期与静止期中,变异链球菌和乳酸杆菌仅存在数量上的区别,二者的单独致龋能力均在活动期增强,但共同致龋作用与龋病的活动与静止无关联。     

Streptococcus mutans in plaque and saliva and the development of caries

Abstract— Plaque samples from 10 different tooth surfaces of 10 schoolchildren with Varied Caries experience (DFS 10-33) were collected five times during 2.5 years. The samples were examined with an immunofluorescent technique for identification and enemeration of Sreptococcus mutans scrotypes C/e/f and d/g. At each sampling occasion the children were scored for caries. A Stimulated saliva sample was also collected and the number of S. mutans per ml salva was determined. The salva level of S. Mutans was Shown to reflect the prevalence and proportion of this microorganism on theselected surfaces. Five surfaces carried S. nutans at each sampling. Four of these surfaces showed progressive caries. S. mutans infection was also found to precede the development of incipient caries on four surfaces. Eighty percent of the surfaces that stayed sound were only transiently carrriers of S. mutans in mainly very low numbers. Serotype c/e/f dominated in prevalence and proportion on the surfaces with a history of caries during the study.