复方嗜酸乳杆菌联合常规四联疗法治疗幽门螺旋杆菌感染消化性溃疡的疗效

目的 观察复方嗜酸乳杆菌联合常规四联疗法治疗幽门螺旋杆菌（Hp）感染消化性溃疡的疗效。方法 选择2018年5月~2019年5月在我院诊治的幽门螺旋杆菌感染消化性溃疡患者112例,采用随机数字表法分为对照组和观察组,各56例。对照组采用常规四联疗法治疗,观察组在对照组基础上加用复方嗜酸乳杆菌治疗,比较两组临床治疗总有效率、临床症状（反酸、上腹痛、饱胀、呕吐）评分、Hp根除率以及临床不良反应发生情况。结果 观察组治疗总有效率为94.64%,高于对照组的80.35%,差异有统计学意义（P＜0.05）;观察组反酸、上腹痛、饱胀、呕吐症状评分均低于对照组,差异有统计学意义（P＜0.05）;观察组Hp根除率为92.85%,高于对照组的78.57%,差异有统计学意义（P＜0.05）;观察组不良反应发生率为3.57%,低于对照组的16.07%,差异有统计学意义（P＜0.05）。结论 复方嗜酸乳杆菌联合常规四联疗法治疗Hp感染消化性溃疡疗效确切,可促进Hp根除,有助于减轻临床症状,降低临床症状评分,且临床不良反应少。     

炭疽芽胞杆菌基因组中串联重复序列拷贝数的分析

目的研究炭疽芽胞杆菌基因组中多位点串联重复序列遗传标记的遗传变异规律。方法根据文献上发表的串联重复序列位点，利用已发表的13对引物，对88株炭疽芽胞杆菌基因组DNA进行PCR扩增，扩增产物进行琼脂糖和聚丙烯酰胺凝胶电泳，利用凝胶影像分析软件对PCR产物DNA片段的碱基含量进行测算，计算出串联重复拷贝数。对部分PCR扩增产物DNA片段进行测序，利用DNAStar软件进行序列比对，分析不同菌株间的串联重复序列遗传变异规律。结果（1）炭疽芽胞杆菌基因组中的串联重复序列位置，同一菌株具有相对稳定的串联重复拷贝数。（2）88株炭疽芽胞杆菌DNA上的13个串联重复序列遗传标记中，有的串联重复序列拷贝数变异较小，有的却存在着复杂的多态性；（3）有些串联重复序列单位内的核苷酸数目不变，但核苷酸的排列组合有差异，不过其重复单位内都包含着一个相同的稳定不变的核心核苷酸序列。结论炭疽芽胞杆菌基因组DNA中串联重复序列可以精确定位，测定结果可以数值化，并且串联重复序列在菌株DNA中具有相对的稳定性，因此，串联重复序列是研究炭疽芽胞杆菌遗传特征的较好指标，具有鉴别炭疽芽胞杆菌菌株间差别的能力。     

蜡样芽胞杆菌DNA分子分型研究

目的 建立蜡样芽胞杆菌分子分型方法，用于蜡样芽胞杆菌食物中毒的快速溯源。方法15株蜡样芽胞杆菌进行生化分型，同时进行vrrA基因PCR扩增和聚丙烯酰胺凝胶电泳-银染检测PCR扩增产物，所有PCR扩增产物进行序列分析，并用ClustalW(ebi．ac．uk)分析软件对DNA序列进行同源性比较。结果传统生化分型：15株蜡样芽胞杆菌中12株可分为3个型，3株不能分型；主要生化型为1型、2型和4型。分子分型：15株蜡样芽胞杆菌都可分为7个型。结论、vrrA基因可作为蜡样芽胞杆菌分子分型的一个多态性遗传标记。蜡样芽胞杆菌分子分型方法与传统生化分型方法相比，将传统生化分型所需的48h甚至更长时间缩短到5h，具有简便快速准确的优点，可做到快速溯源。     

牛源地衣芽胞杆菌诱导小鼠临床乳房炎模型中热休克蛋白和核因子-κB表达上调

目的:为进一步认识奶牛乳腺炎发生的免疫病理机制和寻找更理想的防治方法提供基础研究数据.方法:从采集回来的奶牛奶样中用传统细菌培养方法和分子学方法分离鉴定主要病原菌,挑选其中地衣芽胞杆菌感染小鼠乳腺组织,H-E染色分析感染后小鼠乳腺组织变化,免疫组织化学方法分析热休克蛋白70(HSP70)和核因子-kB(NF-kB)在感染小鼠体内的表达.结果:感染后小鼠乳腺出现明显的炎症症状,组织病理学观察显示腺泡腔内有大量嗜中性粒细胞浸润.实验组中HSP70和NF-kB表达量比生理盐水组和空白组均有增加.结论:成功通过地衣芽胞杆菌诱发建立实验性乳房炎小鼠病理模型,实验组中HSP70和NF-kB表达量的增加说明其参与细菌感染小鼠乳腺组织后的病理过程.     

四联疗法治疗幽门螺旋杆菌相关性十二指肠球炎疗效分析

目的 比较标准四联与三联法治疗幽门螺旋杆菌相关性十二指肠球炎临床疗效。方法 选择2017年5月~2018年5月我院收治的幽门螺旋杆菌相关性十二指肠球炎患者96例,随机分为观察组和对照组,每组48例。观察组采用兰索拉唑、克拉霉素、阿莫西林、果胶铋标准四联治疗,对照组采用兰索拉唑、克拉霉素、阿莫西林标准三联治疗。比较两组患者Hp根除率、复发率及不良反应情况。结果 观察组患者Hp根除率为91.67%,高于对照组75.00%,差异具有统计学意义（P＜0.05）。观察组患者Hp复发率为10.42%,低于对照组的20.83%,差异具有统计学意义（P＜0.05）。观察组不良反应发生率为4.16%,低于对照组的6.25%,但组间比较,差异无统计学意义（P＞0.05）。结论 标准四联疗法治疗幽门螺旋杆菌相关性十二指肠球炎,Hp根除率较高,复发率低,且不增加不良反应发生率。     

蜡样芽胞杆菌溶血性肠毒素的提取及诊断方法的建立

蜡样芽胞杆菌是引起食物中毒的细菌之一〔1〕。蜡样芽胞杆菌的溶血素具有肠毒素的性质〔2〕,提取其溶血素可作为实验室诊断蜡样芽胞杆菌食物中毒和分析食品中残留毒素的诊断试剂。材料和方法蜡样芽胞杆菌溶血素的制备 :试验用的菌株接种肠毒素产毒培养液 ,经 37℃、16ｈ静置培养后 ,振荡培养 10ｈ。培养液基本按Ｂｅｅｃｈｅｒ等〔3〕的方法提取成粗制溶血素。进一步提纯则采用制备性ＳＤＳ ＰＡＧＥ的方法 ,切下含有 3种溶血素成分的凝胶 ,利用蛋白质沉淀或冷冻干燥的方法浓缩后得到分析测定用的溶血素。溶血素的测定1 用紫外吸收法测定蛋…     

幽门螺旋杆菌感染患者与家庭成员共同治疗的疗效研究

目的:观察幽门螺旋杆菌(Helicobacter pylori,HP)感染患者与家庭成员共同治疗的临床疗效。方法:以我院2015年2月~2016年2月期间通过C13呼气试验确诊为幽门螺杆菌感染阳性的患者120例为研究对象,将其随机分为对照组与观察组,每组各60例。对照组采用常规四联疗法治疗;观察组中患者及与患者长期共同生活的C13检测阳性的患者家属均给予常规四联疗法治疗,两组均连续治疗10d后停药1月,再次行C13呼气试验检测比较临床疗效。结果:观察组的根除率为91.7%(55/60),明显高于对照组根除率71.7%(43/60),两组间比较差异显著(P0.05)。结论:HP感染患者与家庭成员共同治疗,可显著提高患者的幽门螺旋杆菌根除率。     

根治幽门螺旋杆菌序贯疗法优于标准药物治疗

意大利S．Orsola医院的DinoVaira医生及其同事4月17日《内科学年鉴》（AnnInternMed，2007；146：556—563．）上报告，对于根治幽门螺旋杆菌，序贯使用几种药物治疗比标准三联药物治疗更加有效。     

一种简易的幽门螺旋杆菌染色法

幽门螺旋杆菌(helicobacter pylori,HP)是一种革蓝染色阴性的螺旋状、微需氧菌.广泛存在于人类尤其是亚洲人的胃液中,是许多慢性胃病发生、发展中的一个重要致病因子.HP的感染率在我国高达50%以上[1],因此,胃黏膜活检后进行HP染色已成为一种常规检测项目,现流行的HP染色是硝酸银法,此银染技术程序复杂.本院现采用的瑞氏染色法,经过多年的实践证明:此方法简单、背景清晰,值得在基层医院推广.     

幽门螺旋杆菌候选疫苗抗原的研究进展

幽门螺旋杆菌(Helicobacter pylori,HP)是1983 年首次从慢性活动性胃炎患者的胃黏膜组织中分离成功,是目前所知能够在人胃中生存的唯一微生物种类,本文对HP 的尿素酶、空泡毒素、细胞毒素、黏附素、过氧化氢酶、膜炎性蛋白、十二指肠溃疡促进因子及脂多糖抗原进行阐述,以便学者对HP 抗原的研究近况进行了解,对未来筛选高效抗原并增加抗原的表达量、探索HP 表位疫苗中表位结合的新型方式等相关研究奠定基础。     

Hp中性粒细胞激活蛋白研究进展

幽门螺杆菌(Hp)是一种参与多种胃、十二指肠疾病的重要人类病原体,可引起慢性胃炎和消化性溃疡,并与胃癌密切相关.人类幽门螺杆菌感染的特征是胃黏膜炎症部位中性粒细胞持续浸润.幽门螺杆菌中性粒细胞激活蛋白(H.pylori neutrophil-activating protein,HP-NAP)正是由于其能活化中性粒细胞而被如此命名.HP-NAP不仅是重要的毒力因子,而且是主要的候选疫苗抗原.本文综述了HP-NAP的结构、生物学活性和致病机制等的研究进展,并对其应用前景进行了展望.     

Helicobacter pylori stains and association between H. pylori and inflammation in gastric specimens

Gastric cancer has been strongly associated with presence of the bacterium Helicobacter pylori. To improve techniques in identifying H. pylori so that gastric cancer may be predicted early, this project was formulated to determine whether one particular stain is more effective in displaying H. pylori microscopically. In addition, this study attempted to determine whether the degree of inflammatory elements present in tissue could be used to predict the likelihood of H. pylori presence. Protocols for the staining techniques, Steiner and alcian yellow/toluidine blue (AY/TB), were employed on specimens to semi-quantitate H. pylori presence. Serial sections from the same specimens were stained with hematoxylin and eosin to determine the amount of inflammation. Spearman rho correlation was used to evaluate the association between amount of H. pylori and inflammation in each case. It was determined that AY/TB was more easily performed, more effective in demonstrating H. pylori, and more cost effective than the Steiner stain. Additionally, it was determined that a moderate positive association was indicated between high levels of inflammation and marked presence of H. pylori.     

The study of H. pylori putative candidate factors for single- and multi-component vaccine development

Helicobacter pylori has grown to colonize inside the stomach of nearly half of the world’s population, turning into the most prevalent infections in the universe. Medical care failures noticeably confirm the need for a vaccine to hinder or deal with H. pylori. This review is planned to discuss the most known factors as a vaccine candidate, including single (AhpC, BG, CagA, KatA, Fla, Hsp, HWC, Lpp, LPS, NAP, OMP, OMV, SOD, Tpx, Urease, VacA) and multi-component vaccines. Many promising results in the field of single and multivalent vaccine can be seen, but there is no satisfactory outcome and neither a prophylactic nor a therapeutic vaccine to treat or eradicate the infection in human has been acquired. Hence, selecting suitable antigen is an important factor as an appropriate adjuvant. Taken all together, the development of efficient anti-H. pylori vaccines relies on the fully understanding of the interactions between H. pylori and its host immune system. Therefore, more work should be done on epitope mapping, analysis of molecular structure, and determination of the antigen determinant region as well due to design a vaccine, preferably a multi-component vaccine to elicit specific CD4 T-cell responses that are required for H. pylori vaccine efficacy.     

Resistance mechanisms of Helicobacter pylori and its dual target precise therapy

Helicobacter pylori drug resistance presents a significant challenge to the successful eradication of this pathogen. To find strategies to improve the eradication efficacy of H. pylori, it is necessary to clarify the resistance mechanisms involved. The mechanisms of H. pylori drug resistance can be investigated from two angles: the pathogen and the host. A comprehensive understanding of the molecular mechanisms of H. pylori resistance based on both pathogen and host would aid the implementation of precise therapy, or ideally “dual target precise therapy” (bacteria and host-specific target therapy). In recent years, with increased understanding of the mechanisms of H. pylori resistance, the focus of eradication has shifted from disease-specific to patient-specific treatment. The implementation of “precision medicine” has also provided a new perspective on the treatment of infectious diseases. In this article, we systematically review current research on H. pylori drug resistance from the perspective of both the pathogen and the host. We also review therapeutic strategies targeted to pathogen and host factors that are aimed at achieving precise treatment of H. pylori.     

抗幽门螺杆菌牛奶的制备及鉴定

目的:制备用于防治H pylori感染的抗 H pylori免疫牛奶。方法:用全体活 H pylori作为抗原,采用皮下多点注射免疫的方法免疫健康奶牛,经过若干次免疫,获得抗H pylori的多克隆免疫牛奶。结果:用直接凝集法和双向琼脂扩散法测得免疫牛奶最高效价分别达到了 1:2 048和 1:32。结论:所制备的免疫牛奶具有多克隆性和很高的效价。     

H. pylori is related to NAFLD but only in female: A Cross-sectional Study

Background: Recently, an increasing number of studies have focused on the extragastrointestinal effects of Helicobacter pylori (H. pylori), including metabolic syndrome, fatty liver, and rheumatic and skin diseases. Nonalcoholic fatty liver disease (NAFLD) is a common chronic disease worldwide that conveys a heavy economic burden on patients and society. The aim of this study was to investigate the relationship between H. pylori and NAFLD and to identify potential influencing factors.Methods: We conducted a cross-sectional study of individuals who had undergone regular physical examinations at the Beijing Shijitan Hospital Health Examination Center from July to October 2018. We evaluated the associations between NAFLD and NAFLD with H. pylori infection and related serum markers using multiple linear regression and logistic regression.Results: There were significant relationships between H. pylori infection status and NAFLD in females (P=0.034) but not in males (P=0.795) according to Fisher''s exact test. The association persisted after further adjustment for metabolic variables, gastrin factors, and liver enzymes. Waist-to-Hip Ratio, Body Mass Index, triglycerides, High-density lipoprotein cholesterol, glucose, uric acid, alkaline phosphatase, and Alanine aminotransferase are related to NAFLD after adjusting for age or interaction between biochemical indexes.Conclusion: H. pylori infection is related to NAFLD in female patients. The relationship between H. pylori and NAFLD may be mediated by markers of lipid metabolism and glycometabolism.     

Ultrastructure of the H. pylori Microbe in Individuals Having Macrocytosis and B12 Deficiency

The incidence of Helicobacter pylori gastritis is high in India and the number of individuals with vitamin B₁₂ deficiency is also large. An association has been found between these two factors. It is necessary to determine whether H. pylori infection may be a factor in the causation of B₁₂ deficiency and whether it is associated with any morphological changes on ultrastructural examination. A cohort-based study has been performed, which includes 505 young asymptomatic males. These cases have been investigated for presence of H. pylori and macrocytosis. The study confirms an association between H. pylori infection and B₁₂ deficiency. It is recommended that H. pylori infection be looked for in subjects having macrocytosis of unknown etiology.     

Influence of IL-1 gene cluster polymorphisms on the development of H. pylori associated gastric ulcer

BACKGROUND AND AIMS: Chronic H. pylori infection is the main cause of ulcer disease which depicts a major burden of our healthy care systems. The individual host immune response plays a pivotal role in the outcome of the infection but genetic susceptibility to develop gastric ulcer is unknown. IL-1beta and its natural receptor antagonist IL-1ra are involved in the inflammatory response to H. pylori infection. Thus, we investigated the influence of functional genetic variants in the IL-1 gene cluster on the development of gastric ulcer disease. PATIENTS AND METHODS: 390 H. pylori infected patients were genotyped for IL-1B -31 and +3954 by TaqMan technology. Alleles of IL-1RN 86VNTR were determined by gel electrophoresis after amplification. Three hundred and sixty healthy blood donors were included as healthy controls. RESULTS: Carriage of the IL-1B -31 C allele conferred a increased but not significant risk for H. pylori infection (OR: 1.3, Wald 95% CI: 0.8相似文献   

纳洛酮联合亚低温疗法基础治疗脑出血的临床效果观察

目的 观察纳洛酮联合亚低温疗法对脑出血进行基础治疗的临床效果,以及血清氧化应激产物在治疗前后的变化。方法 选取2015年12月~2016年10月我院收治的脑出血患者80例,随机分为亚低温疗法组和联合组,每组40例。亚低温疗法组采用亚低温疗法治疗,联合组采用纳洛酮联合亚低温疗法治疗。比较两组的临床治疗效果、治疗前后血清氧化应激产物水平和美国国立卫生研究院卒中量表（NIHSS）评分的变化。结果 联合组的临床总有效率为90.00%,高于亚低温治疗组的77.50%,差异有统计学意义（P＜0.05）。治疗后,两组患者血清SOD、MDA水平均较治疗前降低,且联合组的SOD和MDA水平均低于亚低温治疗组,差异有统计学意义（P＜0.05）。经治疗后,两组患者NIHSS评分均降低,且联合组低于亚低温治疗组,差异有统计学意义（P＜0.05）。结论 纳洛酮联合亚低温疗法治疗急性脑出血,临床疗效显著,可有效降低患者血清氧化应激产物水平,改善患者神经功能状态。     

IFN-gamma synergizes with TNF-alpha but not with viable H. pylori in up-regulating CXC chemokine secretion in gastric epithelial cells.

Helicobacter pylori colonizes the gastric epithelial surface and induces epithelial cells to increase production of the neutrophil attractant IL-8. Little is known about the role of the gastric epithelium in regulating mucosal T cell trafficking. We therefore characterized constitutive and regulated epithelial expression of the CXC chemokines IP-10, I-TAC and Mig, which specifically attract CXCR3 expressing CD4(+) T cells. Human gastric epithelial cell lines (AGS, Kato III, NCI) were used to characterize the constitutive and regulated expression of three CXC chemokines in response to IFN-gamma, TNF-alpha and different H. pylori preparations. Chemokine mRNA and protein production were measured by RT-PCR and ELISA. Gastric epithelial cells constitutively expressed mRNA for IP-10, Mig and I-TAC. IFN-gamma in combination with TNF-alpha strongly induced secretion of those chemokines. Soluble or membranous fractions of H. pylori significantly inhibited IFN-gamma/TNF-alpha induced epithelial cell IP-10 and Mig production. Gastric epithelial cells may contribute to mucosal T cell trafficking. The capacity of H. pylori products to inhibit IP-10 and Mig secretion may explain, at least in part, the failure to induce protective immunity against this bacterium and the ability of H. pylori to affect the presentation of the local inflammation.