SPME-GC-MS 法测定冷却肉的挥发性成分

对固相微萃取(SPME)萃取条件进行优化,并利用SPME 对冷却肉的挥发性成分进行提取。应用SPME 结合气相色谱- 质谱(GC-MS)联用技术初步鉴定冷却肉的挥发性成分,并分析其在不同贮藏温度以及不同贮藏时间下挥发性成分的气味变化。结果表明：在35℃萃取温度和40min 萃取时间的条件下,宜采用65μm PDMS/DVB 萃取头对冷却肉的挥发性成分进行萃取;经SPME-GC-MS 检测后,共获得25 种挥发性成分,并发现醇类物质和三甲胺的相对含量会随着贮藏时间的变化而不断增加。     

顶空固相微萃取-气质联用法分析稻谷挥发性成分

用顶空固相微萃取(HS-SPME)技术结合气相-质谱法(GC-MS)对不同稻谷中挥发性成分进行研究,优化顶空固相微萃取的条件,包括平衡时间、萃取时间、萃取温度以及样品量,并对不同稻谷中挥发性成分进行鉴定和分类分析。结果表明：稻谷中挥发性成分分析的最佳条件为平衡时间60min、萃取时间50min、萃取温度80℃、样品量20g。稻谷中的挥发性成分有醇类、醛类、酮类、酯类、烃类、有机酸类以及杂环类化合物等。最主要挥发性成分是醛类,其中含量最高是己醛,平均为13.31%;其次为壬醛,平均为7.93%。     

SPME/GC-MS对4种超微粉碎杂粮粉中挥发性成分分析

通过固相微萃取-气质联用(SPME/GC-MS)的方法对4种经过超微粉碎技术获得的杂粮(青稞、荞麦、红豆和薏苡)粉中的挥发性成分进行研究,在最优条件下进行检测分析。结果表明,最佳SPME条件为萃取温度80℃、平衡时间10 min、萃取时间40 min、解析时间180 s;GC-MS共检测到并鉴定出的挥发性成分共127种,其中杂环类和其他化合物(92.2%)含量最高,其次是酯类(53.8%)、苯环类(41.8%)化合物。它们共有的挥发性风味物质有11种,分别是壬醛、癸醛、(Z)-6,10-二甲基-5,9-十一碳烯-2-酮、棕榈酸甲酯、棕榈酸乙酯、正十二烷、环十四烷、3’-甲氧基-2,2’-二硝基苯乙炔、2,6-二叔丁基对甲酚、1-甲基萘、2,3-二甲基萘。     

顶空固相微萃取-气质联用技术检测油菜籽中 挥发性成分

目的 采用气相色谱-质谱(GC-MS) 技术对正常油菜籽与霉变油菜籽中挥发性成分进行鉴定和分类分析。方法 采用顶空固相微萃取(HS-SPME)技术对油菜籽中的挥发性成分进行萃取,优化顶空固相微萃取的微萃取头、萃取温度、萃取时间和解析时间等条件,并结合气相色谱-质谱(GC-MS) 技术对正常油菜籽与霉变油菜籽中挥发性成分进行测定。结果 顶空固相微萃取的最佳条件为固相萃取头30/50 μm DVB/CAR/PDMS,萃取温度60 ℃,萃取时间60 min,解吸时间3 min。在最佳实验条件下,GC-MS鉴定出油菜籽中挥发性成分主要有醛类、酮类、醇类、烃类、少量酸、酯类物质及杂环类等多种组分,其中烃类含量最高; 3-辛酮、苯乙酮、苯乙醛、苯乙醇、以及十九烷 、二十一烷等烷烃类化合物仅在霉变油菜籽中检出。结论 该方法可较好区分正常油菜籽与霉变油菜籽。     

顶空固相微萃取-气质联用技术检测油菜籽中挥发性成分

目的采用气相色谱-质谱(GC-MS)技术对正常油菜籽与霉变油菜籽中挥发性成分进行鉴定和分类分析。方法采用顶空固相微萃取(HS-SPME)技术对油菜籽中的挥发性成分进行萃取,优化顶空固相微萃取的微萃取头、萃取温度、萃取时间和解析时间等条件,并结合气相色谱-质谱(GC-MS)技术对正常油菜籽与霉变油菜籽中挥发性成分进行测定。结果顶空固相微萃取的最佳条件为固相萃取头30/50μmDVB/CAR/PDMS、萃取温度60℃、萃取时间60 min、解吸时间3 min。在最佳实验条件下,GC-MS鉴定出油菜籽中挥发性成分主要有醛类、酮类、醇类、烃类、少量酸、酯类物质及杂环类等多种组分,其中烃类含量最高;3-辛酮、苯乙酮、苯乙醛、苯乙醇、以及十九烷、二十一烷等烷烃类化合物仅在霉变油菜籽中检出。结论该方法可较好地区分正常油菜籽与霉变油菜籽。     

红烧猪肉工艺优化及其挥发性风味成分的分离与鉴定

基于模糊数学感官评价法优化红烧猪肉制作工艺参数(焯水时间、焖焅温度、焖焅时间)。采用固相微萃取(SPME)预处理与气相色谱-质谱(GC-MS)联用技术相结合对红烧猪肉中挥发性风味成分进行分离鉴定。结果表明,红烧猪肉的最佳工艺条件为:焯水时间3 min,焖焅温度100℃,焖焅时间2.5 h。对SPME萃取参数(平衡时间、萃取温度、萃取时间)进行优化,确定较佳的萃取条件是:水浴温度60℃下平衡10 min,萃取35 min。通过SPME-GC/MS共鉴定出60种挥发性风味成分,包括醇类10种、醛类10种、脂肪烃类15种、酯类5种、酮类7种、含S、N化合物7种及其它化合物6种。红烧猪肉挥发性风味成分中醛类比例最高(24.68%),其次含S、N化合物(20.99%)、脂肪烃类(19.57%)、醇类(16.88%),而酯类、酮类及其它化合物比例较低(10%),其中己醛(0.72μg/g)、柠檬烯(0.51μg/g)、硫化丙烯(0.51μg/g)为含量较高的化合物。     

烤制鹌鹑蛋挥发性成分SPME条件优化

以鹌鹑蛋为原材料,研究了烤制鹌鹑蛋挥发性成分的固相微萃取(SPME)条件.通过单因素和正交实验对萃取头种类、样品萃取量、萃取时间、萃取温度以及解吸时间进行优化.结果表明,较佳的萃取条件为:DVB/CAR/PDMS萃取头,萃取时间为30min,萃取温度为65℃,样品萃取量为2g,解析时间为2min.在此条件下,检测到烤制鹌鹑蛋中的挥发性成分34种.     

SPME和P&T对西瓜汁香气成分萃取条件优化及二者的比较分析

为了充分萃取西瓜汁的香气成分,避免萃取方法及条件对西瓜汁香气成分分析的影响,本研究优化了前处理方法中PT的时间和温度、SPME中的萃取纤维、温度和时间,并将这两种方法的萃取效果进行了比对分析。研究表明,PT的平衡温度50℃、吹扫时间30min能达到最佳的萃取效果;SPME优化的最佳萃取条件是:CAR/DVB/PDMS纤维、平衡温度40℃、平衡时间40min。这两种方法的对比研究表明,SPME对西瓜汁的萃取效果明显优于PT。     

SPME-GC-MS分析与鉴别青海亚麻籽油挥发性组分

以青海不同产地的40种亚麻籽为原料,索氏抽提提取亚麻籽油,采用顶空固相微萃取结合气相色谱-质谱联用对亚麻籽油挥发性组分进行分析,通过优化搅拌速率、萃取温度、平衡时间、萃取时间、解吸时间、升温程序确定最佳固相微萃取条件及GC-MS条件。结果表明,采用DVB/CAR/PDMS萃取头,亚麻籽油SPME最佳条件为磁力搅拌速率400 r/min,萃取温度80 ℃,平衡时间20 min,萃取时间40 min,解吸时间5 min,在该萃取条件下可以检测出青海40种亚麻籽油挥发性成分共58种,醛类12种,酸类8种,醇类9种,酮类2种,酯类5种,烷烯类13种,杂环类3种,其他类6种,分离鉴定效果较好;根据聚类分析,醛类物质是将亚麻籽油样品进行区分的主要挥发性组分,该结果为亚麻籽油品质鉴定及开发利用提供了理论依据。     

SPME-GC-MS法鉴定养殖大黄鱼主要挥发性风味物质的条件优化

以养殖大黄鱼为研究对象,对其主要挥发性风味物的SPME-GC-MS分析条件进行优化,通过不同的固相微萃取条件的选择、GC-MS条件优化和样品处理的研究,结果表明,SPME-GC-MS最佳分析条件为:大黄鱼样品量5.00g,NaCl添加量5.0%(0.25g),气相程序升温速率8℃/min,选用DVB/CAR/PDMS萃取头,萃取温度70℃,萃取时间30min,平衡时间20min,解析时间5min。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press     

INFOPRINT 2014 was Successfully Held in Dongguan

Sponsored by Printing and Printing Equipment Industries Association of China （PEIAC） and organized by Print China magazine, the Seventeenth Beijing International Printing Information Conference （INFOPRINT 2014） was successfully held on 11th Dec. 2014 at Dongguan Exhibition International Hotel.