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梅俊杰 《医学分子生物学杂志》1998,(4)
由于IL-5受体拮抗剂对哮喘等慢性过敏性疾病具有潜在的应用前景,近年对IL-5及其受体的分子生物学进行了细致深入的研究。本文就IL-5及其受体的基因结构和分子特性、二者作用的分子机制及其信号转导方面的研究作一综述。 相似文献
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人 IL- 6及其受体拮抗剂的研究主要集中于两个方面 :单克隆抗体和突变体。针对人 IL- 6和人 IL- 6 R的活性区域构建的单克隆抗体对于临床治疗多发性骨髓瘤显示出了很好的短期疗效 ,根据与生长激素 (GH)及其受体复合物 GH/ (GHbp) 2的结构对比 ,推测人 IL- 6和 IL- 6 Rα的活性位点 ,结合定点突变技术 ,设计 IL- 6突变体、IL- 6 R突变体和 IL- 6突变体 - IL- 6 Rα融合蛋白 ,它们对天然 h IL- 6的生物活性显示出了明显的拮抗作用 相似文献
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白细胞介素及其受体研究概况 总被引:4,自引:0,他引:4
已被发现和命名的白介素有19种,其相应的受体大多也已被克隆与鉴定。白介素及其受体的结构特征决定了其信号转导和功能效应的共性和特性。许多白介素及可溶性受体在临床疾病的治疗中可能起重要作用,同时也带动了对白介素及其受体的基因工程研究。随着基因组计划的进展和一些新的分子生物学技术方法的建立,关于白介素及其受体的研究在许多方面都面临着新的机遇和挑战。 相似文献
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蛋白酶激活受体激活对A549细胞IL-28和IL-29 mRNA表达的影响 总被引:1,自引:0,他引:1
目的用蛋白酶和蛋白酶激活受体激动肽(PAR-AP)刺激A549细胞后检测细胞中白细胞介素IL-28和IL-29 mRNA表达情况以分析蛋白酶激活受体激活对人肺上皮细胞IL-28和IL-29基因表达的影响。方法用最佳工作浓度的凝血酶、胰蛋白酶、类胰蛋白酶及蛋白酶激活受体-1,2,3,4的激动肽刺激A549细胞后,分别在第2、8、16h收集细胞,用实时定量逆转录聚合酶链式反应(q-RT-PCR)方法分析A549细胞内IL-28和IL-29 mRNA表达。结果凝血酶作用A549后,细胞内IL-29 mRNA表达增强,高达对照组9.6倍;而细胞内IL-28 mRNA表达无明显变化。胰蛋白酶作用A549细胞后,细胞内IL-29和IL-28 mRNA表达增强,分别为对照组6.1倍和4.4倍。类胰蛋白酶作用A549细胞后,细胞内IL-29和IL-28 mRNA表达增强分别高达对照组的3.1倍和2.1倍。弹性蛋白酶作用A549细胞后,细胞内IL-29 mRNA表达增强高达对照组5.1倍而IL-28 mR-NA无明显变化。PAR-1AP(SFLLR)作用后,A549细胞内IL-29 mRNA表达增强为对照组1.7倍而IL-2... 相似文献
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目的 :探讨了丙型肝炎患者血清中TNF、IL - 6、IL - 10水平及意义。方法 :分别应用ELISA法和放免法检测了 5 8例丙型肝炎患者血清中TNF、IL - 6、IL - 10水平 ,并与 35名正常健康人作比较。结果 :丙型肝炎患者血清中TNF、IL - 6、IL - 10水平均非常显著地高于正常人水平 (P <0 .0 1) ,肝硬化组为甚 ,且TNF与IL - 6、IL - 10呈正相关 (r =0 .6 135 ,0 .6 2 2 5 ,P <0 .0 1)。结论 :TNF、IL - 6、IL - 10在丙型肝炎病毒感染的致病机理中有一定的临床价值。 相似文献
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白细胞介素-2及其受体与肿瘤基因治疗 总被引:4,自引:0,他引:4
唐建武 《医学分子生物学杂志》1995,(1)
作为最重要的细胞因子之一,白细胞介素-2(IL-2)抗瘤作用的研究一直引导着肿瘤生物免疫治疗的方向。本文从分子生物学和细胞生物学角度介绍IL-2的结构、激活信号系统、功能活性、各免疫细胞的IL-2分泌动力学特点;IL-2受体亚单位组成、表达调控、功能意义及在不同细胞中的表达状况;IL-2基因转导接种疗法的基本方法、作用原理、主要优点、近年获得的实验与临床研究数据、以及待解决的问题等。 相似文献
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过敏性紫癜患儿血清IL-8和IL-12的水平及其意义 总被引:2,自引:0,他引:2
目的:旨在探讨IL-8和IL-12在AP和在APN发病机制中的作用及其相互关系,期望在AP的治疗方面提供一些新的理论依据。方法:检测32例AP、11例APN和15例正常儿童血清中IL-8、IL-12的变化,并分析它们之间的相关性意义。结果:过敏性紫癜患儿急性期血清中IL-8、IL-12的含量无论普通组还是肾损害组均显著高于对照组,恢复期IL-8和IL-12仍高于正常对照组。急性期IL-8和IL-12呈正相关性。说明二者共同参与了过敏性紫癜的发病过程。结论:本实验说明了IL-8和IL-12共同参与了过敏性紫癜的发病过程,尤其在紫癜性肾炎中,为IL-8、IL-12拮抗剂在过敏性紫癜治疗中的应用提供了客观依据。 相似文献
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白细胞介素-15(IL-15)是一种新发现的细胞因子,其生物学性质与IL-2相似。IL-15的受体由IL-15Rα、IL-2Rβ、IL-2Rγ组成,其中IL-2Rγ是参与IL-15信号传递过程的必要成分。IL-15和IL-2的体内表达及受体分布存在差异预示着IL-15在体内具有独特的生物学作用。 相似文献
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IL-3是一种造血生长因子,通过其受体α和β两条链向细胞内传递信号。为研究IL-3,IL-3Rα,β三者之间的相互作用模式,利用大肠杆菌系统表达了4种IL-3Rα胞外片段:IL-3R-P(氨基酸1~307),IL-3R-2(1~263),nIL-3R-2(1~75),cIL-3R(105~263)。活性研究发现,除nIL-3R-2外,其余3个片段对IL-3依赖性细胞株TF-1有不同程度的刺激活性,并且FITC标记的IL-3R-P可与TF-1细胞结合,βc真核表达质粒转染CTLL细胞后,FITC标记的IL-3R-P可与之结合。实验结果提示IL-3Rα链存在潜在的β链结合位点,一旦暴露可以激活β链传递信号。 相似文献
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目的:探讨Th17细胞分泌的细胞因子IL-17、IL-21和IL-22在血清中的表达与儿童支气管哮喘的相关性以及与儿童哮喘发作程度的关系。方法:采用ELISA法,对68例哮喘患儿和30例健康对照患儿血清中IL-17、IL-21和IL-22的水平进行检测。患儿血清中IL-17、IL-21和IL-22与患儿性别、年龄的关系进行Pearson相关分析。结果:三种细胞因子的水平在不同性别间无显著性差异,与年龄亦无相关性。哮喘患儿血清中IL-17(37.35±10.52)pg/mL和IL-22(407.42±137.41)pg/mL的表达水平明显高于健康对照组,而IL-21表达水平在两组之间无差异;IL-21和IL-22的表达与哮喘发作程度无关,而IL-17表达水平与哮喘发作程度呈正相关。结论:Th17细胞分泌的细胞因子IL-17和IL-22在哮喘的炎症中发挥重要作用,是监测病情和诊断哮喘的重要指标之一。 相似文献
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目的 研究Toll样受体9(toll-like receptor 9,TLR9)基因启动子区单核苷酸多态性在浙江汉族儿童中的分布,探讨其与哮喘易感性及其表型之间的相关性.方法 对312例变应性哮喘患儿(哮喘组)和339名健康儿童(对照组)采用DNA直接测序法检测TLR9基因-1486(rs187084)和-1237(rs5743836)单核苷酸多态性;采用ELISA法检测两组不同基因型血清干扰素γ(interferon-γ,IFN-γ)、白细胞介素12(interleukin-12,IL-12)和白细胞介素4(interleukin-4,IL-4)水平;采用化学发光法检测血清总免疫球蛋白E(immunoglobulin E,IgE)水平;采用酶免疫荧光法检测血清变应原特异IgE.结果 (1)哮喘组和对照组均存在-1486位点T→C突变,哮喘组TT、TC和CC 3种基因型的频率分别是3 8.8%、48.4%和12.8%,对照组分别是41.0%、44.3%和14.7%;未发现-1237位点存在多态性.(2)哮喘组和对照组-1486位点各基因型的频率分布差异无统计学意义(P>0.05),年龄分层后比较差异也无统计学意义(P>0.05).(3)哮喘组-1486位点3种基因型的血清IFN-γ和IL-4水平差异有统计学意义(P<0.01),CC基因型的IFN-γ水平较低而IL-4水平较高;对照组2种细胞因子的差异无统计学意义(P>0.05).哮喘组和对照组血清IL-12水平在3种基因型间差异均无统计学意义(P>0.05).(4)哮喘组-1486位点不同基因型血清总IgE水平差异无统计学意义(P>0.05).结论 浙江汉族儿童不存在TLR9基因-1237位点多态性.TLR9基因-1486 C/T位点单核苷酸多态性与浙江汉族儿童哮喘易感性、血清IL-12及总IgE水平无关;-1486 C/T位点多态性与哮喘患儿血清IFN-γ和IL-4水平有关联,CC基因型的IFN-γ水平较低而IL-4水平较高.Abstract: Objective To investigate the distribution characteristics of the single nucleotide polymorphisms (SNPs) in the promoter region of the toll-like receptor 9 gene (TLR9)in Chinese Han children from Zhejiang province, and their associations with asthma susceptibility and phenotypes. Methods A case-control study was conducted. A total of 312 asthmatic children aged between 1.9 and 11.6 and 339 age matched healthy controls were enrolled in this study from April 2007 to November 2008. The -1486 C/T in rs187084 and -1237 C/T in rs5743836 loci of the TLR9 gene were genotyped by direct DNA sequencing of the PCR products. Serum levels of IFN-γ, IL-12 and IL-4 were detected by enzyme linked immunosorbent assay. Serum levels of total IgE were detected by chemiluminescence, and serum levels of ildren (P<0.01). The CC genotype had the lowest levels of serum IFN-γand the highest levels of serum IL-4 among the three genotypes. There were no significant differences in these cytokines among the healthy controls (P>0.05). No statistical differences of serum IL-12 were found among the three genotypes in the two groups (P>0.05). (4) There were no significant differences of total IgE (log-transformed) among the three genotypes in the asthmatic children (P>0. 05). Conclusion The -1237 C/T polymorphism of TLR9 gene was not detected in Chinese Han children in this study. The - 1486 C/T polymorphism was associated with the levels of serum IFN-γ and IL-4 in children with asthma.However, there were no correlations between the -1486C/T polymorphism and serum IL-12 levels, total IgE levels or asthmatic susceptibility. 相似文献
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慢性肺心病急性发作期淋巴细胞IL-2R及转铁蛋白受体的变化 总被引:2,自引:0,他引:2
应用Wu系列单克隆抗体(McAb),对25例慢性肺心病急性发作期患者淋巴细胞白细胞介素2受体(IL-2R)、转铁蛋白受体(TfR)及T淋巴细胞亚群进行检测。结果表明,慢性肺心病急性发作期患者IL-2R与TfR均低于正常对照组(P<0.001,P<0.05),其CD_4细胞亦较正常对照组为低(P<0.005),CD_8细胞在两者间无明显差异(P>0.10)。患者PaO_2与IL-2R的表达是高度正相关(r=0.82,P<0.001),提示血氧水平可影响淋巴细胞IL-2R的表达。慢性肺心病急性发作期IL-2R、TfR降低及CD_4细胞减少的意义有待进一步研究。 相似文献
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CD4+T细胞在免疫应答反应中发挥关键作用,CD4+T细胞根据其分泌的细胞因子的不同分为多种细胞亚群.其中辅助性T细胞9(Th9)细胞是最近发现的一种新型的CD4+效应T细胞,该细胞由Na(i)ve CD4+T细胞分化而来,可分泌特征性的细胞因子白细胞介素(IL)-9.Th9细胞的发育和分化具有独立的调节机制,其在过敏性炎症、自身免疫病和抗肿瘤免疫中发挥着重要作用.然而,Th9细胞在免疫应答反应中的调控,与其它CD4+T细胞亚群的相互作用及在疾病中的效应作用等问题仍需深入研究. 相似文献
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Toll样受体9基因启动子单核苷酸多态性与儿童变应性哮喘的相关性 总被引:1,自引:0,他引:1
Objective To investigate the distribution characteristics of the single nucleotide polymorphisms (SNPs) in the promoter region of the toll-like receptor 9 gene (TLR9)in Chinese Han children from Zhejiang province, and their associations with asthma susceptibility and phenotypes. Methods A case-control study was conducted. A total of 312 asthmatic children aged between 1.9 and 11.6 and 339 age matched healthy controls were enrolled in this study from April 2007 to November 2008. The -1486 C/T in rs187084 and -1237 C/T in rs5743836 loci of the TLR9 gene were genotyped by direct DNA sequencing of the PCR products. Serum levels of IFN-γ, IL-12 and IL-4 were detected by enzyme linked immunosorbent assay. Serum levels of total IgE were detected by chemiluminescence, and serum levels of ildren (P<0.01). The CC genotype had the lowest levels of serum IFN-γand the highest levels of serum IL-4 among the three genotypes. There were no significant differences in these cytokines among the healthy controls (P>0.05). No statistical differences of serum IL-12 were found among the three genotypes in the two groups (P>0.05). (4) There were no significant differences of total IgE (log-transformed) among the three genotypes in the asthmatic children (P>0. 05). Conclusion The -1237 C/T polymorphism of TLR9 gene was not detected in Chinese Han children in this study. The - 1486 C/T polymorphism was associated with the levels of serum IFN-γ and IL-4 in children with asthma.However, there were no correlations between the -1486C/T polymorphism and serum IL-12 levels, total IgE levels or asthmatic susceptibility. 相似文献
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Objective To investigate the distribution characteristics of the single nucleotide polymorphisms (SNPs) in the promoter region of the toll-like receptor 9 gene (TLR9)in Chinese Han children from Zhejiang province, and their associations with asthma susceptibility and phenotypes. Methods A case-control study was conducted. A total of 312 asthmatic children aged between 1.9 and 11.6 and 339 age matched healthy controls were enrolled in this study from April 2007 to November 2008. The -1486 C/T in rs187084 and -1237 C/T in rs5743836 loci of the TLR9 gene were genotyped by direct DNA sequencing of the PCR products. Serum levels of IFN-γ, IL-12 and IL-4 were detected by enzyme linked immunosorbent assay. Serum levels of total IgE were detected by chemiluminescence, and serum levels of ildren (P<0.01). The CC genotype had the lowest levels of serum IFN-γand the highest levels of serum IL-4 among the three genotypes. There were no significant differences in these cytokines among the healthy controls (P>0.05). No statistical differences of serum IL-12 were found among the three genotypes in the two groups (P>0.05). (4) There were no significant differences of total IgE (log-transformed) among the three genotypes in the asthmatic children (P>0. 05). Conclusion The -1237 C/T polymorphism of TLR9 gene was not detected in Chinese Han children in this study. The - 1486 C/T polymorphism was associated with the levels of serum IFN-γ and IL-4 in children with asthma.However, there were no correlations between the -1486C/T polymorphism and serum IL-12 levels, total IgE levels or asthmatic susceptibility. 相似文献