血清酸溶液性蛋白测定在肿瘤诊断上的价值

藉改良的考马斯亮蓝染料结合法,对正常成人,良性肿瘤患者和恶性肿瘤患者共430例进行血清酸溶性蛋白测定。结果表明,血清酸溶性蛋白测定在恶性肿瘤筛查、诊断和良、恶性肿瘤鉴别上有一定的临床实用价值。     

Further Observations on the Radiology of Intra-Uterine Foetal Blood Transfusions

Techniques for localizing the foetal peritoneum, inserting a needle and through it a catheter, and finally confirming the correct placement of the catheter have been described and discussed. The need for keeping the radiation dosage to mother and foetus as low as possible and the importance of beam restriction in this regard have been stressed. Very small foetuses present special problems, but size alone is no contra-indication for treatment.     

Dietary Protocatechuic Acid During the Progression Phase Exerts Chemopreventive Effects on Chemically Induced Rat Tongue Carcinogenesis

The modifying effects of dietary administration of protocatechuic acid (PCA) during the progression phase of ‍tongue carcinogenesis initiated with 4-nitroquinoline 1-oxide (4-NQO) were investigated in male F344 rats. For ‍tumor progression we developed a new animal model, where rats initiated by 4-week treatment of 20 ppm 4-NQO in ‍drinking water, received four cycles of 20 ppm 4-NQO to induce advanced tongue cancer (one cycle: 2 weeks of 4- ‍NQO followed by 2 weeks of tap water), starting at 14 weeks after the initiation. In this model, metastasis of tongue ‍cancer occurred in lungs. Starting two weeks before the cycle treatment with 4-NQO, animals were fed the 2000 ppm ‍PCA containing diet and continued on this diet until the end of the study. At the termination of the experiment (week ‍32), the incidences of tongue neoplasms and preneoplastic lesions, polyamine levels in the tongue tissue, and cell ‍proliferation activity estimated by morphometric analysis of silver-stained nucleolar organizer regions’ protein were ‍compared among the groups. Feeding with PCA containing diet during the progression phase significantly decreased ‍the occurrence of advanced tongue squamous cell carcinoma with metastasis (P<0.05) and preneoplasia (hyperplasia ‍and dysplasia) (P<0.001). In addition, PCA exposure decreased polyamine levels in the tongue tissue (P<0.001) during ‍progression phase. Our results suggest that dietary PCA inhibits progression of 4-NQO-induced oral carcinogenesis, ‍and such inhibition might be related to suppression of cell proliferation by PCA. ‍     

姜黄素对马兜铃酸诱发的膀胱肿瘤的预防作用

 目的 以马兜铃酸诱发的膀胱癌为模型，观察姜黄素对膀胱组织的病理变化、Ras蛋白和p53蛋白及细胞角质蛋白20（CK20）的变化，评价姜黄素对马兜铃酸诱导膀胱癌的化学预防作用及发生机制。方法 50只大鼠随机分为对照组、诱癌组、预防组三组。诱癌组：给予10mg（kg·d）马兜铃酸给大鼠灌胃,连续诱癌3个月；预防组：在诱癌的同时给予含2%姜黄素粉的饲料进行化学防护；对照组：为正常饮食和饮水。3个月后宰杀全部大鼠，取膀胱组织进行HE染色显微镜下观察病理变化，用免疫组化染色技术检测膀胱组织内Ras、p53蛋白的变化，荧光定量PCR技术检測CK20 mRNA。结果 经3个月诱癌，膀胱癌的发生率在诱癌组为95% (19／20)，而姜黄素预防组，膀胱癌的发生率仅为10%（2/20），对照组膀胱黏膜组织Ras、p53蛋白及CK20 mRNA均呈阴性表达，预防组及诱癌组膀胱黏膜组织Ras、p53蛋白及CK20 mRNA均呈阳性，且以诱癌组更明显，差异具有统计学意义（P<0.001）。结论 姜黄素对马兜铃酸诱导的膀胱癌具有良好的化学预防作用，可能分子机制与抑制 Ras、p53蛋白的过度表达能力有关。姜黄素有望成为一种有应用前景的膀胱癌预防药物。