共查询到20条相似文献，搜索用时 15 毫秒
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Ying Shu Shaobin Yu Ling Zha Ping Fu Tianlei Cui 《Hemodialysis international. International Symposium on Home Hemodialysis》2017,21(4):E66-E68
Fungal endocarditis (FE) is commonly regarded as a rare but fatal disease. The incidence of infective endocarditis (IE) in hemodialysis (HD) patients is thought to be obviously higher than that in the general population. Moreover, IE occurs more likely in HD patients with catheters. With the increase of HD population and extensive use of catheters in HD patients, FE, as a special form of IE, may increase and bring new challenges to clinicians. We reported a case of FE associated with catheter infection in a 44‐year‐old woman on HD. The risk factors and treatment strategies of FE in HD patients were discussed. 相似文献
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Chung‐Te Liu Yen‐Cheng Chen Tso‐Hsiao Chen Tsong‐Yih Ou 《Hemodialysis international. International Symposium on Home Hemodialysis》2012,16(4):564-567
Necrotizing fasciitis is a lethal soft tissue infection for its rapid progression to septic shock. We present a 59‐year‐old male on chronic hemodialysis (HD). We made the diagnosis of necrotizing fasciitis of the right thigh due to the crepitus from physical examination and subcutaneous emphysema from an X film. He was successfully treated with antibiotics and surgical debridement. The blood and surgical drainage cultures showed Escherichia coli, which is less commonly seen in cutaneous infection. The colonoscopic finding revealed adenomatous polyps. Necrotizing fasciitis in patients on HD requires early diagnosis and aggressive treatment to ensure the favorable clinical outcomes. 相似文献
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Farin Rashid‐Farokhi Guitti Pourdowlat Mohammad‐Reza Nikoonia Neda Behzadnia Shahram Kahkouee Amir‐Ahmad Nassiri Mohammad‐Reza Masjedi 《Hemodialysis international. International Symposium on Home Hemodialysis》2013,17(1):94-100
Chronic hemodialysis (HD) patients are predisposed to several complications associated with pleural effusion. In addition, uremia can directly cause pleuritis. However, there are inadequate data about pathogenesis and natural course of uremic pleuritis. In this study, 76 chronic HD patients with pleural effusion admitted to the Respiratory Center of Masih Daneshvari Hospital, in Tehran, Iran between June 2005 and May 2011 were evaluated to figure out the etiology of their pleural disease. Among these patients, patients with uremic pleuritis were identified and studied. The rate of uremic pleuritis was 23.7%. Other frequent etiologies of pleural effusion were parapneumonic effusion (23.7%), cardiac failure (19.7%), tuberculosis (6.6%), volume overload, malignancy, and unknown. In patients with uremic pleuritis, dyspnea was the most common symptom, followed by cough, weight loss, anorexia, chest pain, and fever. Compared to patients with parapneumonic effusion, patients with uremic effusion had a significantly higher rate of dyspnea and lower rate of cough and fever. Pleural fluid analysis showed that these patients had a significantly lower pleural to serum lactic dehydrogenase ratio, total pleural leukocytes, and polymorphonuclear count compared to patients with parapneumonic effusion. Improvement was achieved in 94.1% of patients with uremic pleuritis by continuation of HD, chest tube insertion or pleural decortication; an outcome better than the previous reports. Despite the association with an exudative effusion, inflammatory pleural reactions in patients with uremic pleuritis may not be as severe as infection‐induced effusions. Owing to the advancement in HD technology and other interventions, outcome of uremic pleuritis may be improved. 相似文献
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纳米银对大肠杆菌的抗菌作用及其机制 总被引：11，自引：0，他引：11
以大肠杆菌为研究对象,对纳米银的抗菌效果进行了研究.并对其抗菌机制做了初步探讨.纳米银对大肠杆菌的抑制生长曲线的结果表明,20 μg/mL的纳米银能够完全抑制106 cfu/mL的大肠杆菌细胞生长,纳米银使大肠杆菌的延滞期加长,并且纳米银浓度越高,延滞期越长.采用透射电镜观察了经纳米银粒子处理过的大肠杆菌细胞形态变化过程,结果显示纳米银粒子先在细胞壁上产生小的孔洞,通过这些孔洞进入周质空间,导致细胞膜成分渗漏和破坏细胞膜,进而进入细胞内部.进入细胞内部的纳米银粒子使DNA浓缩呈紧张态,并与破损细菌的细胞质结合积聚,最后引起胞内物质流失.另外,纳米银对大肠杆菌总DNA影响的分析表明.随着纳米银浓度的增高,大肠杆菌总DNA样品降解的程度增大. 相似文献
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用一种新的蛋白质纯化流程提纯由大肠杆菌表达的夏氏疟原虫AMA1片段。大肠杆菌表达的片段首先用镍柱提纯,提纯后的蛋白用DTT还原,对盐酸胍透析,再对空气氧化。用RP－HPLC对片段二次提纯,通过冻干转换缓冲液。SDS－PAGE、RP－HPLC和质谱分析都显示,经这种纯化流程提纯的蛋白有很高的纯度,且二硫键已完全正确形成。提示这一蛋白质纯化流程可用于由大肠杆菌表达的低分子量寡二硫键的蛋白。 相似文献
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表达harpin基因的大肠杆菌DH5（pCPP430）诱导植物抗病性的研究 总被引：10，自引：0，他引：10
用表达ｈａｒｐｉｎ基因的大肠杆菌ＤＨ５进行了诱导植物抗病性的研究，发现表达ｈａｒｐｉｎ基因的重组菌除可明显诱发烟草、番茄叶片的过敏反应外，对玉米、水稻叶片也可诱发过敏反应，反应比相应阳性对照病菌速度快，坏死斑也更典型。用ＤＨ５菌悬液喷雾或注射诱导后，番茄对早疫病、水稻对稻瘟病、玉米对大、小斑病、马铃薯对软腐病的抗性均有不同程度的提高，与水杨酸诱导的抗性效果相当或比其略高。 相似文献
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环境因素对细菌附着的影响 总被引：1，自引：0，他引：1
生物污损是船舶及海洋工程面临的主要问题,其中由细菌附着形成的生物膜难以避免,且具有介导无脊椎污损生物幼虫附着的特性,尤为棘手。因此,控制海洋细菌生物膜的形成成为防污研究的重要课题。本文利用海洋细菌Bacillus subtilis和Escherichia coli设计试验,考察环境因素和基底材料对细菌生物膜形成的影响,揭示细菌生物膜的形成规律。研究结果表明:温度、盐度等环境因素对Bacillus subtilis和Escherichia coli附着形成生物膜的影响极显著(方差检验结果即P<0.01),通过控制环境因素可以干扰生物膜的形成。 相似文献
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Po‐Jen Hsiao Ming‐Hsien Tsai Jyh‐Gang Leu Yu‐Wei Fang 《Hemodialysis international. International Symposium on Home Hemodialysis》2015,19(2):330-332
Iliopsoas abscess is a rare complication in hemodialysis patients that is mainly due to adjacent catheterization, local acupuncture, discitis, and bacteremia. Herein, we report a 47‐year‐old woman undergoing regular hemodialysis via a catheter in the internal jugular vein who presented with low back pain and dyspnea. A heart murmur suggested the presence of catheter‐related endocarditis, and this was confirmed by an echocardiogram and a blood culture of methicillin‐resistant Staphylococcus aureus. A computed tomography indicated a pulmonary embolism and an incidental finding of iliopsoas abscess. Following surgical intervention and intravenous daptomycin, the patient experienced full recovery and a return to usual activities. This case indicates that an iliopsoas abscess can be related to a jugular vein catheter, which is apparently facilitated by infective endocarditis. The possibility of iliopsoas abscess should be considered when a hemodialysis patient presents with severe low back pain, even when there is no history of adjacent mechanical intervention. 相似文献
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Rameez Ahmed Ankita Vaishampayan Jose Luis Cuellar‐Camacho Darren J. Wight Ievgen Donskyi Wolfgang Unger Elisabeth Grohmann Rainer Haag Olaf Wagner 《Advanced Materials Interfaces》2020,7(15)
Aiming at the overall negative surface charge of bacteria, a new strategy of antibacterial agents based on large polymer‐modified graphene oxide (GO) sheets is assessed. The presented flexible, polycationic sheets match the size and charge density of the Escherichia coli surface charge density (2 × 1014 cm−2). These matching parameters create an unspecific but very strong bacteria adsorber by multivalent, electrostatic attraction. Their interaction with bacteria is visualized via atomic force and confocal microscopy and shows that they effectively bind and wrap around E. coli cells, and thereby immobilize them. The incubation of Gram‐negative and ‐positive bacteria (E. coli and methicillin‐resistant Staphylococcus aureus, MRSA) with these polycationic sheets leads to the inhibition of proliferation and a reduction of the colony forming bacteria over time. This new type of antibacterial agent acts in a different mode of action than classical biocides and could potentially be employed in medicinal, technical, or agriculture applications. The presented microsheets and their unspecific binding of cell interfaces could further be employed as adsorber material for bacterial filtration or immobilization for imaging, analysis, or sensor technologies. 相似文献
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Sarva Mangala Praveena Lim Suk Han Leslie Thian Lung Than Ahmad Zaharin Aris 《Journal of Experimental Nanoscience》2016,11(17):1307-1319
This study investigates the ability of cellulose filter paper coated with silver nanoparticles to remove Escherichia coli from drinking water. The cellulose filter paper was coated with silver nanoparticles by a chemical reduction method using two different ratios of sodium borohydride and silver nitrate. In consideration of drinking-water quality standards and non-carcinogenic health risks, the optimum sodium borohydride:silver nitrate ratio for coating the cellulose filter paper was determined by comparing the silver in the effluent after E. coli removal. For both ratios, 100% E. coli removal was realised. In terms of the silver in the effluent, only the first two lowest concentrations for both ratios of sodium borohydride and silver nitrate were compliant with the drinking-water quality standards, demonstrating hazard quotients (HQs) between 0.084 and 0.484. On the basis of the highest level of E. coli removal with the lowest HQ value, the optimum sodium borohydride:silver nitrate ratio for coating the cellulose filter paper as an antibacterial water filter was 2:1 molar ratio (0.002 M:0.001 M). Silver nanoparticle-coated cellulose filter paper was found to be an inexpensive and easy-to-use emergency antibacterial water filter to generate clean drinking water. 相似文献
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本文以大肠杆菌为转化菌,研究了海藻酸钠包埋法细胞固定化技术,初步探讨了细胞固定化方法对产天冬氨酸酶活稳定性的影响。将固定化细胞放置于不同条件下保存,对比研究保存前后的酶活力,结果表明：海藻酸钠浓度4％,细胞浓度5％,温度40℃时保存的固定化细胞酶活稳定性最高。 相似文献
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Kristine Opsvik Wikene Anne Bee Hegge Ellen Bruzell Hanne Hjorth Tønnesen 《Drug development and industrial pharmacy》2015,41(6):969-977
Context: Bacterial resistance to antibiotics is increasing and alternative antibacterial treatments like antimicrobial photodynamic therapy (aPDT) are needed. Curcumin is under investigation as a potential photosensitizer in aPDT.Objective: The purpose of this study was to develop rapidly dissolving formulations of curcumin that could photoinactivate both Gram-positive and Gram-negative bacteria.Materials and methods: Curcumin solid dispersions with methyl-β-cyclodextrin and hyaluronic acid (HA), hydroxypropyl methylcellulose (HPMC) or both HA and HPMC were prepared through lyophilization. The lyophilizates were characterized by curcumin drug load [% (w/w)], differential scanning calorimetry, photostability, thermal stability, their ability to form supersaturated solutions and by in vitro photoinactivation of Enterococcus faecalis and Escherichia coli.Results and discussion: The lyophilizates were amorphous solid dispersions with a curcumin drug load in the range of 1.4–5.5% (w/w) depending on the included polymer and the ratio between curcumin and the cyclodextrin. The lyophilizates were photolabile, but thermally stable and dissolved rapidly in contact with water to form supersaturated solutions. Selected lyophilizates demonstrated >log 6 reduction of colony forming units/ml of both E. faecalis and E. coli after exposure to low curcumin concentrations (0.5–10?µM) and blue light dose (11–16?J/cm2). The high drug load of the lyophilizates, rapid dissolution, ability to form relatively stable supersaturated solutions and the very high phototoxicity towards both E. faecalis and E. coli make these lyophilizates suitable for in vivo aPDT.Conclusions: This treatment with optimized curcumin formulations should be explored as an alternative to topical antibiotics in the treatment of wound infections. 相似文献
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枯草芽孢杆菌植酸酶基因在大肠杆菌中的表达及其对酶热稳定性的影响 总被引：9，自引：0，他引：9
采用PCR法,获得不含有信号肽序列的来源于枯草芽孢杆菌的植酸酶phyC基因的非融合和融合表达片段,分别构建带有T7lac启动子的大肠杆菌的植酸酶pET30NFphyC和pET30FphyC表达载体,并转入大肠杆菌BL21(DE3)。大肠杆菌分别在30℃和25℃经IPTG诱导后实现了植酸酶的表达,非融合和融合植酸酶的表达量分别约占菌体总蛋白的13％和15％,分子量分别为40．13kDa和43．27kDa。表达产物具有植酸酶的生物学活性,非融合植酸酶和融合植酸酶的最适反应温度分别为50℃和75℃,经90℃处理10min,残留酶活性分别为37℃时的31．9％和75．7％。分析表明,含13个氨基酸残基的融合片段有助于植酸酶的表达和酶热稳定性的提高。 相似文献
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为了能够现场快速特异性检测大肠杆菌,研制了基于生物发光磁酶免疫的便携式检测仪,检测仪由光子计数单元、中央处理单元、温度控制单元和电源管理单元组成.温度控制单元可以提供恒定的温度37℃,适于培养和现场检测.提出一种改进的、将免疫磁珠与生物素化兔抗大肠杆菌抗体偶联的检测方法,能够一步直接测定大肠杆菌浓度.对浓度为23.00 CFU/mL～2.25×107CFU/mL的大肠杆菌菌液进行快速检测,仪表检测值与传统培养法结果在对数下呈良好的线性关系,相关系数为0.965 7,系统的检测灵敏度为0.478 7.对样品进行重复性测试,变异系数为4.48%.该集成了温度控制单元的检测仪便携、易于操作、检测成本低,将在食品卫生和安全、环境监测、疾病诊断等领域具有潜在的应用前景. 相似文献
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Characterization and Photocatalytic Efficiency of TiO2 /Ti Beads Fabricated by Simple Heat-Treatment
Altangerel Amarjargal Leonard D.Tijing Mi-Hwa Yu Chae-Hwa Kim Chan-Hee Park Dong-Won Kim Cheol-Sang Kim 《材料科学技术学报》2012,28(2):184-192
The goal of the present study was to investigate the photocatalytic efficiency of titanium dioxide (TiO 2) formed on titanium (Ti) bead substrate (referred herein as TiO 2 /Ti beads) by heat treatment when exposing to ultraviolet (UV) light irradiation.Escherichia coli was used as the model test organism.The results show 4-log and 7-log decrease in bacterial concentration after a test time of 15 and 120 min,respectively,using TiO 2 /Ti beads irradiated with UV light in a tin-foil covered beaker.This article presents the potential of TiO 2 on Ti bead substrate formed by simple heat-treatment together with UV light for bacterial inactivation. 相似文献
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《Journal of Experimental Nanoscience》2013,8(4):299-309
The primary aim of the study was to observe the morphological changes taking place on Escherichia coli when treated with non-cationised placebo lipidic emulsion (NCPLE) and cationised placebo lipidic emulsion (CPLE). The particle size of NCPLE and CPLE indicated the mean diameter of 10.77 ± 0.20 nm and 56.98 ± 1.718 nm, respectively. The zeta potential of NCPLE and CPLE depicted the potential of ?10.8 ± 0.80 mV and +20.9 ± 3.25 mV, respectively. Agar diffusion study indicated higher inhibition zone of 21.4 ± 0.85 mm for CPLE as evaluated against 14.7 ± 0.76 mm for NCPLE. Transmission electron micrographs of Escherichia coli treated with NCPLE and CPLE showed the potential of blank nanoemulsions as antibacterial, which can be used as a therapeutic agent. The bacterial cell being negatively charged attracts the cationised nanoemulsions from the bulk of dispersion. As the concentration of nanoemulsion increased in the vicinity of the bacterial cell, the degree of collisions increased between the cationised nanoemulsion leading to coalescence and the formation of a pool of emulsion containing bacterial cells leading to cell death. The study demonstrated that placebo lipidic nanoemulsion (NCPLE & CPLE) may itself be used as an active therapeutic agent for the treatment of bacterial infections. Moreover, it can also be used as a carrier system for antibacterial drugs for the two-pronged synergistic effect. 相似文献
