激光—血卟啉衍生物与癌地龙合用对癌细胞的杀伤效应

用~3H脱氧胸腺嘧啶核苷(~3H-TdR)参入DNA合成的生化学方法和癌细胞形态学方法观察到,不同方法处理腹水型(H_(22))肝癌细胞,其杀伤率,A组(912-HpD-laser)为74.4％;B组(HpD-912)为59.7％;C组(laser)为20.4％;D组(912)为27.0％;E组为0％作为对照。A组与其它组相比,杀伤作用显著增强(P均<0.01)。又通过测定脂质过氧化代谢产物Schiff碱的量观察各组对癌细胞的杀伤作用。Schiff碱有荧光特性,荧光强度越大,Schiff碱含量越高,癌细胞死亡数越多,上述5组的荧光强度测定结果分别是84.6,41.6,45.1,61.1和35.2mm。A组显著高于其它各组(P均<0.01)。进一步用化学发光法证明,912-激光-血卟啉衍生物杀伤癌细胞过程中有过氧化氢产生。上述结果提示,912-激光-血卟啉衍生物杀伤癌细胞的作用与活性氧的生成及其介导的脂质过氧化有密切关系。     

肝癌细胞膜脂胆固醇含量对膜结合血卟啉衍生物及膜流动性的影响(论著摘要)

应用等电聚焦电泳分离结合血卟啉衍生物(HpD)的血清,用苏丹黑预染脂蛋白和HpD光照后产生红色荧光,证明HpD与血清脂蛋白和清蛋白部分相结合。制备34种不同卵磷脂/胆固醇摩尔比(PL/C,Mol/Mol)的脂质体,即1:0、1:0.4、1:1.5和1:2。将4种脂质体分别直接与定量的HpD 37℃温育。测定HpD-脂质体的荧光光谱和荧光强度,结果其荧光光谱与HpD-肝癌细胞的荧光光谱相同,最大荧光峰在630mm处,且其荧光强度随脂质体中胆固醇相     

几种血卟啉衍生物制剂对癌细胞的杀伤效应以及单线态氧的产生和在癌细胞内的集落

HpD-81为北京医工所供给,HpD-82为南京中心血站供给,HpD-83为自制。癌细胞置日光灯下2厘米处照射30分钟。HpD-81对几种癌细胞株的半数杀伤剂量是;S-180细胞4.2(μg/ml下同),μ14细胞3.5,EAC细胞1.7,Eca109细胞3.2,HpD-82:EAC细胞 4.7,Eca109细胞10.0。三种HpD制剂对小     

912对血卟啉衍生物-激光抗癌的增敏作用及其机理

作者用化学发光法为主要指标,辅以癌细胞死亡率和形态学观察,研究了912和血卟啉衍生物(HpD)加激光对离体腹水瘤S_(180)细胞系杀伤的协同作用及其机理.1 材料和方法 腹水瘤S_(188)细胞系在LACA小鼠腹腔内繁殖,接种后6d,拉颈处死取出腹水分离细胞,以含10％小     

南岳毛尖抑制T_(739)小鼠移植性肺癌生长作用的实验研究

本研究用30％湖南南岳毛尖(一级绿茶)浸泡液灌胃处理皮下种植性肺腺癌795(LA_(795))的T_(739)近交系小鼠,观察其抗癌效果。结果表明:该绿茶可明显抑制肺腺癌795的生长,抑瘤率达70.51％,与对照组比较P<0.001;瘤块周围出血例数、癌细胞核分裂数明显少于对照组(P<0.01,P<0.001),空泡变性癌细胞数明显少于对照组(P<0.001)。说明南岳毛尖有较好的抗癌作用。     

复方斑蝥胶囊体内外抗肿瘤作用的实验研究

目的:通过体内外抗肿瘤实验观察复方斑蝥胶囊的抗肿瘤作用。方法:建立小鼠移植性肝癌(H_(22))和白血病(L_(1210))荷瘤动物模型,观察药物对荷瘤动物的抑瘤作用和生命延长作用,对环磷酰胺(Cy)的协同抑瘤作用;采用染料排斥法检测药物在不同浓度及不同作用时间下对靶细胞的杀伤作用,测出其半数杀伤浓度(IC_(50))。结果:复方斑蝥胶囊对H_(22)可产生显著的抑瘤作用;高剂量可明显增加环磷酰胺的抗癌效果,但对L_(1210)荷瘤动物的生存时间无显著延长。体外对H_(22)及L_(1210)的IC_(50)分别为(21.83±6.14)μg·ml~(-1)和(100.23±30.3)μg·ml~(-1)。结论:复方斑蝥胶囊在体内及体外均对H_(22)表现出明显的抑瘤作用,对L_(1210)作用不明显。     

不同波长的光源对血卟啉衍生物(HpD)产生光氧化作用的影响(论著摘要)

近年来,应用血卟啉(HpD)加激光的光动力疗法(PDT)治疗肿瘤,在临床上取得较大的进展.由于激光的穿透组织能力有限,因此,试图在PDT治疗中以穿透力强的X线代替激光照射瘤体以提高PDT疗效.PDT是基于HpD的光敏作用,为此本实验以X线、紫外光、黑光及激光四种不同波长的光源照射在加有HpD的肝癌细胞,观察对肝癌细胞膜所产生的光氧化作用来比较四种光源对HpD所产生的光敏效应.     

HpD—激光对动物瘤体的细胞化学影响(选用细胞色素氧化酶、琥珀酸脱氢酶、酸性磷酸酶显示法)

本文为研究HpD-激光破坏恶性肿瘤的机理,采用细胞化学方法观察了小白鼠肿瘤中细胞色素氧化酶、琥珀酸脱氢酶和酸性磷酸酶的活性。在HpD-激光处理瘤细胞后12小时细胞色素氧化酶活性明显降低,24小时即消失。然而,琥珀酸脱氢酶和酸性磷酸酶无明显变化。结果表明,HpD-激光破坏恶性肿瘤的原因之一,乃是抑制了瘤细胞内的细胞色素氧化酶活性,进而阻断了呼吸链。     

激光光路净化装置

我们在应用HpD-激光治疗恶性肿瘤中,发现HpD比较稳定,而激光则复杂,功率变化较大,直接影响到治疗效果。我们在未采用激光光路净化装置前,一般20～30分钟后功率就下降100mW左右;应用净化装置后,在数小时内可使激光功率基本保持不变,从而提高了疗效。本文报告激光光路净化装置的制作方法及应用该装置前、后激光功率的稳定情况。     

光动力疗法及其联合尼美舒利对QBC939细胞caspase-3活性的影响

目的 研究光动力疗法(PDT)及其联合尼美舒利对人胆管癌细胞株QBC939 caspase-3活性的影响,探索PDT及其联合尼美舒利杀伤人胆管癌细胞可能的作用机制.方法 以血卟啉衍生物(HpD)为光敏剂,以630 nm波长红激光为光源,将HpD孵育的QBC939照射激光,再给予尼美舒利处理,用分光光度法检测QBC939细胞caspase-3的活性变化.结果 HpD-PDT作用QBC939细胞后能早期激活caspase-3,效应呈剂量依赖性,与时间呈负相关;与单纯应用HpD-PDT或尼美舒利相比,两者联合作用QBC939细胞后,caspase-3的活性升高更明显,活化时间更长.结论 caspase-3的激活可能是HpD-PDT杀伤人胆管癌细胞的机制之一,可能是HpD-PDT联合尼美舒利杀伤人胆管癌细胞的共同通路.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.