桂花品种的ISSR-PCR分析

利用ISSR—PCR方法对桂花的54个品种进行了基因组多态性分析,从70个ISSR引物中筛选出13个多态性引物用于正式扩增,共扩增出90条DNA片段,其中多态性DNA带79条,占总扩增片段的87.8％,平均每个引物扩增的DNA带的数目为6.92条。依据扩增结果,应用RAPD Distans软件进行Nei相似性系数和遗传距离计算,利用UPGMA法构建聚类树状图。结果表明:ISSR分析中产生了一些品种特有的指纹图谱,因此ISSR技术在桂花品种和品种群(品系)的鉴定和阐明遗传关系中有很大的应用潜力。利用DNA扩增结果进行聚类分析,把供试桂花的54个品种分为7个大类,并对品种进行了处理及种下品种群的遗传关系进行了探讨。     

桂花品种的ISSR-PCR分析

<正>利用ISSR—PCR方法对桂花的54个品种进行了基因组多态性分析,从70个ISSR引物中筛选出13个多态性引物用于正式扩增,共扩增出90条DNA片段,其中多态性DNA带79条,占总扩增片段的87.8％,平均每个引物扩增的DNA带的数目为6.92条。依据扩增结果,应用RAPD Distans软件进行Nei相似性系数和遗传距离计算,利用UPGMA法构建聚类树状图。结果表明:ISSR分析中产生了一些品种特有的指纹图谱,因此ISSR技术在桂花品种和品种群(品系)的鉴定和阐明遗传关系中有很大的应用潜力。利用DNA扩增结果进行聚类分析,把供试桂花的54个品种分为7个大类,并对品种进行了处理及种下品种群的遗传关系进行了探讨。     

小麦近缘种及小麦-簇毛麦染色体代换系、易位系的SSR分析

用20对小麦微卫星引物，对小麦近缘种和小麦-簇毛麦染色体代换系、易位系的基因组DNA进行PCR扩增，14对引物有扩增产物，其中10对引物可以在小麦近缘种间扩增出多态性标记．共扩增出分子量小于500的条带477条，其中342条具有多态性，每个SSR座位的等位基因数目在2～6个之间，平均为3．7个．11个实验材料的基因多态性(PIC)范围在0．4013～0．7281之间，平均为0．5725．根据NeiM的方法进行遗传距离分析，构建系统树，确立小麦及近缘种的亲缘关系，为麦类作物遗传资源的研究及在育种中的应用提供一些理论依据．     

金佛山特有杜鹃花遗传多样性分析

利用ISSR分子标记初步分析了重庆金佛山特有的4种杜鹃花的亲缘关系,从100个引物中筛选出13个能扩增出清晰条带并具有多态性的引物.共扩增出了239条DNA片段,分子量约为200~1500bp.其中多态性片段约为171条,平均每条引物扩增出18.38条DNA片段.同时采用DPS软件计算出4种杜鹃花的Nei’S遗传距离,并用NTSYSpc2.1中的UPGMA法构建了系统树.结果表明4种杜鹃花之间既有相同的遗传背景,又存在一定的差异.4种杜鹃花明显分为两类:树枫杜鹃为一类,疏花美容杜鹃,金佛山美容杜鹃与阔柄杜鹃为一类.     

大蒜种质资源遗传多样性的分子标记研究

本研究利用RAPD和ISSR两种分子标记技术．对中国10个不同地区的大蒜品种进行了种质资源遗传多样性研究．从30个RAPD引物当中．筛选出11个具有多态性的引物．共扩增出多态性带224条．多态性条带比率为41．18％．从12个ISSR引物中筛选出5个具多态性的ISSR引物．共扩增出多态性带121条．多态性条带比率为50．21％．对RAPD和ISSR两种标记分别运用Nei指数法．计算出10个大蒜品种的平均遗传距离为0．28和0．32．根据这两种标记的结果．采用UPGMA进行聚类分析，得到与生物学分类地位基本一致的结果．结果表明，在实验稳定性上．ISSR优于RAPD．且ISSR比RAPD能检测到更多的遗传变异．RAPD和ISSR两种标记可用于大蒜种质遗传多样性的研究．     

利用ISSR技术对优质红锥种质资源遗传多样性的分析

利用ISSR分子标记对10个优质红锥品种进行基因组多态性分析．从20条引物中筛选的11条具有扩增多态性的引物共扩增出435条带，其中多态性条带325条，占总扩增条带的74．7％，平均每个引物扩增3．95条．ISSR标记揭示的10个优质红锥品种的遗传相似系数变化范围为0．4737～0．8081，平均值为0．7287．聚类分析可将10个优质红锥品种分为四类．     

滩羊体大品系遗传标记的研究

采用RAPD(Rondom Amplified Polymorphic DNA)技术,利用混合基因池(DNA pool)法,对滩羊体大品系、普通品系进行了DNA多态性分析,从100种具有10个碱基的随机引物中,筛选出84种引物在滩羊群体基因组中共扩增出358条带,其中22种引物的扩增产物表现为多态(占22％),且扩增出32条有差异的条带,占总带数的8.94％;62种引物的扩增产物表现为单态(占62％)。滩羊体大品系的特异性条带有5条,而普通品系的特异性条带有7条,这些特异标记可以用来鉴定滩羊的两个品系;滩羊体大品系与普通品系间的遗传距离为0.136±0.087,表明两品系之间的亲缘关系很近。     

基于ISSR的披针莲座蕨居群遗传多样性分析

采用ISSR分子标记对披针莲座蕨的6个自然居群97个样品进行了DNA多态性分析.从38个随机引物中筛选出11个有效引物,扩增共产生81条DNA片段,其中79条为多态性条带,多态位点百分比(PPB)为97.5%.与其他蕨类植物相比,披针莲座蕨居群遗传多样性水平较高.居群遗传关系聚类结果与各居群的地理分布基本一致.     

大蒜种质资源遗传多样性的分子标记研究

本研究利用RAPD和ISSR两种分子标记技术,对中国10个不同地区的大蒜品种进行了种质资源遗传多样性研究.从30个RAPD引物当中,筛选出11个具有多态性的引物,共扩增出多态性带224条,多态性条带比率为41.18%,从12个ISSR引物中筛选出5个具多态性的ISSR引物,共扩增出多态性带121条,多态性条带比率为50.21%.对RAPD和ISSR两种标记分别运用Nei指数法,计算出10个大蒜品种的平均遗传距离为0.28和0.32.根据这两种标记的结果,采用UPGMA进行聚类分析,得到与生物学分类地位基本一致的结果.结果表明,在实验稳定性上,ISSR优于RAPD,且ISSR比RAPD能检测到更多的遗传变异,RAPD和ISSR两种标记可用于大蒜种质遗传多样性的研究.     

榛子种质资源遗传多样性的RAPD分析

利用RAPD分子标记技术，对来自欧洲、美国和我国北方的共48份榛子品种或品系（包括4个种及种间杂种）的遗传多样性进行了分析．从150条随机引物中筛选出24条引物，共扩增出142条清晰可重复的带，其中多态性带130条，比例为91．5％．聚类分析将48份榛子种质分为5组：欧榛、平榛各自列为1组；杂交榛分成2个组；另外一组包括滇榛和藏榛2份种质．研究结果对榛子的种间亲缘关系进行了验证，且为同种不同品种的亲缘关系提供了更为详细的信息．     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Achieving Unusual States of Matter Under High Pressure

正The periodicity of the elements and the non-reactivity of the inner-shell electrons are two related principles of chemistry,rooted in the atomic shell structure.Within compounds,Group I elements,for example,invariably assume the+1 oxidation state,and their chemical properties differ completely from those of the p-block elements.These general rules govern our understanding of chemical structures and reactions.Using first principles calcula-     

Exchange-Correlation and Electronic Excitation Energies from Pairing Matrix Fluctuations

We have developed an adiabatic connection to formulate the ground-state exchange-correlation energy in terms of pairing matrix linear fluctuations.This formulation of the exchange-correlation energy opens a new channel for density functional approximations based on the many-body perturbation theory.We illustrate the potential of such approaches with an approximation based on the particle-particle Random Phase Approximation(pp-RPA).This re-     

Density Functional Theory for the Response of Periodic Systems to Electric Fields Based on the Vector Potential Approach

正The electronic and nuclear(structural/vibrational)response of 1D-3D nanoscale systems to electric fields gives rise to a host of optical,mechanical,spectral,etc.properties that are of high theoretical and applied interest.Due to the computational difficulty of treating such large systems it is convenient to model them as infinite and periodic(at least,in first approximation).The fundamental theoretical/computational problem in doing so is that     

On the Thermodynamic Limit for Responses to Static Electromagnetic Fields

For molecular systems,the quantum-mechanical treatment of their responses to static electromagnetic fields usually employs a scalar-potential treatment of the electric field and a vector-potential treatment of the magnetic field.Although the potential for each field separately is associated with the choice of an(unphysical)origin,the precise choice of the origin for the electrostatic field has little consequences for the results.This is different for the     

An affordable accurate evaluation of the binding conformations predicted by molecular docking studies in bio-system

Franck-Condon factors bridge the gap between theoretical modeling and experimental observations for molecular electronic spectroscopy and electron transfer.Under the displaced harmonic oscillator approximation,multidimensional Franck-Condon factors are decomposed into a product of many one-dimensional(1D)Franck-Condon(FC)factors,and each 1D-FC factor is associated with one Huang-Rhys factor that determines the leading contribution of     

Call for Papers

<正>"The Journal of Shanghai Normal University:Mathematics"is published by Shanghai Normal University as regular issues of The Journal of Shanghai Normal University each year from 2014 in English.The editors-in-chief of the issues are professors Yuhao Cong and Maoan Han.The Journal of Shanghai Normal University was started in 1958 with     

Journal of Donghua University ( English Edition) Contents of Volume 31

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