制备万寿菊叶黄素技术研究进展

由于叶黄素(lutein)是自然界广泛存在的二羟基类胡萝卜素,也是人眼视网膜黄斑色素主要组成部分。叶黄素可有效预防并辅助治疗老年性黄斑退化病和白内障等眼部疾病,其在生物活性物质利用领域有广泛的应用前景。万寿菊是工业上提取分离叶黄素的理想工业原料。本文从万寿菊叶黄素提取专用品种、叶黄素分子结构及叶黄素稳定性等6个方面综述了近年来国内外有关万寿菊叶黄素的研究工作,并进行了前景展望。     

万寿菊干花中叶黄素酯的超声提取工艺研究

研究了超声提取万寿菊干花中叶黄素酯的工艺条件,分别考察了不同溶剂、料液比、超声功率和超声时间对叶黄素酯含量的影响.结果表明:采用正己烷为溶剂,料液比1:30,超声功率500W,超声时间40min为最佳条件,此时叶黄素酯的含量达到15.5mg/g.     

万寿菊干花中叶黄素酯的超声提取工艺研究

研究了超声提取万寿菊干花中叶黄素酯的工艺条件,分别考察了不同溶剂、料液比、超声功率和超声时间对叶黄素酯含量的影响。结果表明:采用正己烷为溶剂,料液比1∶30,超声功率500W,超声时间40min为最佳条件,此时叶黄素酯的含量达到15.5mg/g。      

制备万寿菊叶黄素技术研究进展

由于叶黄素(lutein)是自然界广泛存在的二羟基类胡萝卜素,也是人眼视网膜黄斑色素主要组成部分。叶黄素可有效预防并辅助治疗老年性黄斑退化病和白内障等眼部疾病,其在生物活性物质利用领域有广泛的应用前景。万寿菊是工业上提取分离叶黄素的理想工业原料。本文从万寿菊叶黄素提取专用品种、叶黄素分子结构及叶黄素稳定性等6个方面综述了近年来国内外有关万寿菊叶黄素的研究工作,并进行了前景展望。      

万寿菊干花颗粒中叶黄素酯的超临界CO2萃取初探

从万寿菊花中萃取叶黄素酯是制备叶黄素过程中的重要技术。本项研究对叶黄素酯的超临界CO2萃取进行了初步探索。实验结果表明,提高萃取压力、减小样品颗粒粒径以及减小萃取介质的极性均可增加叶黄素酯的萃取率。今后如果能在破坏叶黄素酯与花瓣组织介质的关联程度和降低萃取介质极性两个方面取得进展,超临界流体萃取有可能成为一种可应用于叶黄素酯萃取的技术。     

万寿菊干花颗粒中叶黄素酯的超临界CO2萃取初探

从万寿菊花中萃取叶黄素酯是制备叶黄素过程中的重要技术。本项研究对叶黄素酯的超临界CO2萃取进行了初步探索。实验结果表明,提高萃取压力、减小样品颗粒粒径以及减小萃取介质的极性均可增加叶黄素酯的萃取率。今后如果能在破坏叶黄素酯与花瓣组织介质的关联程度和降低萃取介质极性两个方面取得进展,超临界流体萃取有可能成为一种可应用于叶黄素酯萃取的技术。      

超声波提取万寿菊中叶黄素的工艺研究

以万寿菊颗粒为原料,乙酸乙酯为提取溶剂,采用超声波辅助的提取方法,主要讨论了温度、时间、料液比、功率对叶黄素提取的影响。研究表明,当温度35℃、时间45min、料液比1︰4、功率120W时叶黄素的提取效果最好。     

万寿菊中叶黄素的生产技术及应用

介绍了万寿菊中叶黄素研究开发现状、化学组成、应用,探讨了万寿菊中叶黄素的提取和纯化方法,展望了万寿菊中叶黄素的开发应用前景。     

万寿菊叶黄素的提取及分析方法研究进展

叶黄素（lutein）是自然界广泛存在的含紫罗酮环的二羟基类胡萝卜素，也是人眼视网膜黄斑色素主要组成部分。由于叶黄素可有效预防并辅助治疗老年性黄斑退化病和白内障等眼部疾病，其在生物活性物质利用领域有广泛的应用前景。万寿菊是工业上提取分离叶黄素的理想工业原料。本文综述了近年来国内外有关万寿菊叶黄素的研究工作，着重介绍叶黄素的新型提取方法及其定性定量分析方法。     

超声波法提取万寿菊中叶黄素的工艺优化

实验以叶黄素提取率为指标,采用正交设计L9(34),考察超声波功率、超卢提取时间、万寿菊粒径、料液比4个因素,对万寿菊(Tagetes erecta L.)花瓣中叶黄素的提取工艺进行优化筛选.结果表明,最佳工艺为超声功率150W,超声时间40min,万寿菊粒径80～100目,料液比1:15.     

万寿菊油树脂制取高纯度叶黄素酯的工艺研究

研究万寿菊油树脂中叶黄素酯的分离纯化过程。结果表明,较优的分离纯化条件为：丙酮溶解万寿菊油树脂的条件为：料液比1:3(m/V)、温度50℃、冷却时间3h;正丁醇处理丙酮浓缩物的条件为：料液比1:3.5(m/m)、温度50℃、冷却时间4h,正丁醇处理物再经乙醇洗涤,真空干燥,得纯度可达85% 以上的叶黄素酯。     

C30柱分离万寿菊花中的叶黄素类化合物初探

应用C30-HPLC-PDA,万寿菊花萃取物中的叶黄素类化合物获得了良好的分离,包括:叶黄素酯的分离和单体几何异构体的分离。色谱条件为:WatersYMCCarotenoidS-5(4.6×250mm)柱;乙腈-甲醇(75:25,V/V)为流动相A,甲基叔丁基醚为流动相B,线性梯度洗脱;流速:1.0ml/min;PDA波长范围:300～600nm;进样量:20μl。根据各组分的色谱行为、光谱特征和在碘催化下发生几何异构的产物分析对各组分进行初步鉴定。实验结果显示:C30固定相在分离万寿菊花萃取物中的叶黄素类化合物的应用中有良好的前景。     

叶黄素的体外抗氧化功能

应用反相高压液相色谱(RP HPLC)从万寿菊的花中制备叶黄素的反式(E )、顺式(Z )异构体及其酯类3个组分。然后,应用微弱发光检测技术测定和比较各组分在体外淬灭单线态氧的能力。结果表明,反式和顺式叶黄素及其酯类均有体外淬灭单线态氧的能力,同时,还可观察到顺式异构体的“延迟发光”现象,说明反式异构体与顺势异构体可能有不同的淬灭单线态氧的机理     

响应面试验优化万寿菊花中叶黄素酯皂化反应工艺

以叶黄素酯为原料,对万寿菊花中叶黄素酯皂化反应的工艺进行优化,考察反应溶剂种类及用量、皂化温度、皂化时间等因素对皂化反应的影响,采用薄层层析法计算皂化率,确定叶黄素酯皂化的适宜操作条件,对皂化反应工艺进行单因素试验和响应面分析研究。结果表明,叶黄素酯的皂化反应最佳工艺条件为叶黄素酯为2.0 g时,无水乙醇用量31.3 mL、皂化温度60 ℃、KOH用量2.1 g、皂化时间4 h,此条件下叶黄素酯的平均皂化率为78.4%。     

万寿菊叶黄素提取分离研究

本文研究了由万寿菊提取叶黄素的条件,包括皂化处理NaOH浓度,提取时间与次数对提取效率的影响,并通过薄层层析对产品进行了分析。     

Stereoisomers Identification and Storage Stability of Microencapsulated Marigold Lutein

Stability of microencapsulated marigold lutein under three different storage conditions (dark and vacuum, light and vacuum, air and light) at 4, 20, and 37°C was investigated. Major lutein stereoisomers, including all-trans lutein, 13-cis lutein, 13′-cis lutein, 9-cis lutein, and 9′-cis lutein, were rapidly separated within 12 minutes by reverse-phase C₃₀-HPLC-PDA using methanol, methylene chloride, and acetonitrile as eluent, which could provide more explicit information for identifying and distinguishing stereoisomers. The content of all-trans lutein decreased during storage time for all the treatments, and the degradation of all-trans lutein fitted the first order model as correlation coefficients (R²) were higher than 0.95, microencapsulated marigold lutein with vacuum packed were preserved best in dark and 4°C storage.     

循环超声法提取万寿菊中叶黄素的研究

研究了循环超声技术在万寿菊中叶黄素提取中的应用.实验以万寿菊发酵造粒为原料,对比了不同提取溶剂的提取效果;实验还采用正交试验研究了料液比、超声波功率、超声作用时间对提取率的影响;比较了超声提取与静态溶剂提取法的提取效果.结果显示,最佳提取溶剂为丙酮∶石油醚=1∶1(V/V);较理想的提取条件为料液比1∶70、超声作用时间为20min、超声功率为800W;在此条件下超声循环提取法的提取率为98%,而溶剂静态浸提法仅为42%.     

Correlation of HPLC and AOAC Methods to Assess the All-trans-Lutein Content in Marigold Flowers

A reversed-phase C₃₀ high-performance liquid chromatography (HPLC) method was used to separate the lutein isomers of marigold flowers. The pigments were extracted from marigold meal by an Association of Official Analytical Chemists (AOAC) modified method. The unsaponified extract showed the presence of several lutein ester isomers and after saponification four major lutein isomers were detected. The relationship between all- trans -lutein, assessed by HPLC, and total xanthophylls, assessed by AOAC, was determined. A regression equation, with a high correlation coefficient ( r ² =0·972), which allows for the calculation of all- trans -lutein from total xanthophylls content is proposed.     

Modulation of Marigold Based Lutein Dye and its Dyeing Behaviour Using UV Radiation

Application of natural dyes has increased interest in the past few years due to the eco-friendly behavior of these dyes. The present research is concerned with the effect of UV on dyeing behavior of cotton using marigold as source of natural Lutein dye. This is colorant lutein which imparts greenish yellow color to cotton fabric. The dye powder and cotton fabric were exposed to UV-radiation for different time intervals prior to dyeing and dyeing was performed at different dyeing variables. International Standard Organization (ISO) methods were employed to evaluate the color fastness properties, such as color fastness to light, washing and rubbing. It is found that 90 min exposure of UV radiations was the optimum condition for surface modification and dyeing of 70 min at 40°C give excellent results using 4 g/L salt to achieve maximum exhaustion. For improvement of color fastness, tannic acid (8%) as pre- and 6% as post-mordant is the best condition. It is found that UV ray treatment can be used to other fabrics followed by dyeing using extracts of dye yielding plants without any physical characteristics damage.