Synthesis of three bromophenols from red algae as PTP1B inhibitors

Bromophenols are a set of natural products widely distributed in seaweed, most of which exhibit interesting and useful biological activities. To develop a reliable and efficient synthetic route to these natural bromophenols, three of them, 3,4-dibromo-5-(2′-bromo-3′,4′-dihydroxy-6′-methoxymethyl-benzyl)-benzene-1,2-diol (compound 9), 3,4-dibromo-5-(2′-bromo-6′-ethoxy methyl-3′,4′-dihydroxybenzyl)-benzene-1,2-diol (compound 10), and 3-bromo-4-(3′-bromo-4′,5′-dihydroxy benzyl)-5-(ethoxymethyl)-benzene-1,2-diol (compound 14), isolated from red marine algae, have been synthesized in eight steps with an overall yield of 14.4%, 14.4%, and 18.2% respectively, via a practical approach employing bromination, Wolff-Kishner-Huang reduction and a Friedel-Crafts reaction as key steps. The protein tyrosine phosphatase 1B (PTP1B) inhibitory activities of the synthetic compounds were evaluated by the colorimetric assay. The results show that these compounds are moderate PTP1B inhibitors. The synthesis of these bromophenol derivatives makes in vivo studies of their structure-activity relationships and inhibition activity against PTP1B possible.     

LET CHANGXING ISLAND BE A WATER SOURCE ISLAND OF SHANGHAI

The reason why the Changxing Island should be the water resource island of Shanghai City is dis-cussed based on the analysis of the natural conditions of the island. Tentative ideas on engineering design and suggestions for pre-engineering study are proposed.     

Screening and Characterization of Nitrite-Degrading Bacterial Isolates Using a Novel Culture Medium

In this study,a novel culture medium that simulates shrimp pond conditions was established to screen nitrite-degrading isolates.The medium was supplemented with nitrite as a nitrogen source and shrimp feed as the major carbon source,to achieve the high nitrogen and low carbon nutritional status found in shrimp farming ponds.Screening using this medium identified potent denitrifying Bacillus isolates,among which Bacillus subtilis M7-1 was considered best.M7-1 was able to completely degrade nitrite-N in 24 h without much consumption of dissolved oxygen.Efficient denitrification activity took place in liquid cultures within a set of non-stringent ranges of pH(5.0–9.0),salinity(0–30)and temperature(25–35℃).The denitrifying enzyme gene was amplified,sequenced and further identified as nirS type.In biosecurity assessments,M7-1 had no negative effects on shrimps at a dose of 106 cfu mL−1.M7-1 could therefore be used in aquaculture to reduce and control the nitrogen concentration,and to promote the development of sustainable and healthy culture systems.     

Seismic waves in a layered half-space from an arbitrary buried source

In this paper, a transfer matrix and a three-dimensional dynamic response of a layered half-space to an arbitrary buried source are derived with the aid of a technique which combines the Laplace and two-dimensional Fourier transforms in a rectangular coordinate system. This method is clear in concept, and the corresponding formulas given in the paper are simple and convenient for marine seismic prospecting and other fields' applications. An example is presented and the calculated results are in good agreement with those of the finite element method (FEM).     

Construction and characterization of a fosmid library for pathogenic bacterium <Emphasis Type="Italic">Vibrio anguillarum</Emphasis>

Vibrio anguillarum is a common bacterial pathogen in fish.However,little is known about its pathogenic mechanism,in part,because the entire genome has not been completely sequenced.We constructed a fosmid library for V.anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage.We characterized the library by end-sequencing 50 randomly selected clones.This generated 93 sequences with a total length of 57 485 bp covering 1.4% of the whole genome.Of these sequences,58...     

Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria

A metagenomic fosmid library of approximately 52 000 clones was constructed to identify functional genes encoding cold-adapted enzymes. Metagenomic DNA was extracted from a sample of glacial meltwater,...     

数字锁相频率源研究与设计

介绍了数字锁相环的基本原理、主要技术指标及集成频率合成器Q3236的功能特点。对Q3236外围电路参数进行了分析设计。同时也对系统性能进行了分析。     

青藏高原大气热源气候特征的研究

用NCEP/NCAR再分析资料和小波分析方法分析研究了1950-2005年青藏高原大气热源气候特征和变化特征,主要结论包括:(1)夏季青藏高原东部大气热源的强度明显较西部大.6月份,高原东部热源的强度是高原西部的近两倍,7月份的值也比西部大了40%以上.(2)青藏高原全区、东部和西部逐年平均的大气热源有明显不同的变化特征.高原全区年平均大气热源的变化主要是一个14年的时间尺度;高原东部不仅有14年的主要时间变化尺度,同时还有一个非常显著的2.6年的时间变化尺度;高原西部则不同,是一个不明显的1-2年的时间尺度.     

Estimation of non-point source pollution loads under uncertain information

Many kinds of uncertainties are involved, such as random, fuzzy, grey, unascertained property and so on, in soil erosion process. To exactly predict the non-point source pollution loads, some uncertainties should be taken into consideration. Aiming at the deficiency of present blind number theory being helpless for fuzziness, a novel blind number, i.e. extended-blind number, was introduced by substituting a set of triangular fuzzy numbers （TFNs）, expressed as a-cuts, for interval values in present blind number, and the expected value of extended-blind number was also brought forward by referring to the current blind number theory. On the basis of denoting the parameters of Uni- versal Soil Loss Equation （USLE） as extended-blind parameters, a novel USLE model was established for quantitatively evaluating soil erosion loss and non-point source pollution loads. As a case, the uncertain USLE was employed for predicting the soil erosion loss and non-point source pollution loads of absorbed nitrogen and phosphorus in a dis- trict in the Hangbu-Fengle River basin, in the upstream of Chaohu Lake watershed. The results show that it is feasible in theory to extend blind number into fuzzy environment and reliable on conclusion to apply extended-blind number theory for predicting non-point source pollution loads.     

基于Smith-Purcell效应的0.257THz辐射源研究

带电粒子束沿光栅表面高速通过时将产生Smith-purcell效应,当电子束与表面光栅的表面波模式同步的情况下表面波将会对电子束进行调制。通过调整表面光栅的参数将使表面波频率落入到THz波段。采用粒子模拟（PIC）对基于Smith-purcell效应的THz波段辐射源进行了研究,适当的选择参数进行优化后,得到了工作在0.257THz调制深度〉10%输出功率为10W的辐射源。     

Geochemical characteristics of Early Cretaceous source rocks in Boli Basin

The Early Cretaceous deposits are composed of important source rocks in Boli Basin. The types of the source rocks include black mudstones and coal （with carbonaceous mudstone）. By the organic geochemical analysis methods, the authors discussed the organic petrological characters, abundance of organic matter, degree of maturity and the type of source rocks. The main micro-component of black mudstone is exinite or vitrinite, and the content of vitrinite is high in coal. The weathering of the outcrop is very serious. The abundance of organic matter in source rock reaches the poor to better rank. The major kerogens in mudstone are type- Ⅲ, type- Ⅱ 2 and some type- Ⅱ 1 ; the organic type of coal is type-Ⅲ. The thermal evolution of the source rocks in every structural unit is very different, from low-maturity to over-maturity. The depositional environment is reductive, which is good for the preservation of organic matter. The organic matter in source rocks is mainly from aquatic organisms and terrigenous input.     

Textural research on the main source of the Changjiang River

The Chinese people began to research the main source of the Changjiang (Yangtze) River 2,400 years ago. Limited by the scientific level, they did not discover it. The Tuotuo River was determined as the main source of the Changjiang River in the 1970s. However, this was not correct, because when comparing the length of the Tuotuo River with the Dam River, the glacier length at the headwaters was added to the Tuotuo River, resulting in that the Tuotuo River is 1 km longer than the Dam River, keeping in mind that the glacier can not be regarded as part of the river. In the summer of 1986, we investigated the source of the Changjiang River, we accurately measured the length of both the Tuotuo and Dam rivers, we discovered that the Dam River was 353.1 km long, and the Tuotuo River was 346.3 km long, the Dam River thus being 6.8 km longer than the Tuotuo River. The discharge of the Dam River is 196.18 m³/sec., 2.6 times as large as that of the Tuotuo River, that of the Tuotuo River is 75.10 m³/ sec. The drainage area of the Dam River is 1.8 times as larger as that of the Tuotuo River; the drainage area of the Dam River is 30,715.7 km², the Tuotuo River is 16,691.0 km². Through synthetic analysis of the factors mentioned above, we came to the conclusion that the main source of the Changjiang River is the Dam River instead of the Tuotuo River.     

Geochemical characteristics of Early Cretaceous source rocks in Boli Basin

The Early Cretaceous deposits are composed of important source rocks in Boli Basin. The types of the source rocks include black mudstones and coal (with carbonaceous mudstone). By the organic geochemical analysis methods, the authors discussed the organic petrological characters, abundance of organic matter, degree of maturity and the type of source rocks. The main micro-component of black mudstone is exinite or vitrinite, and the content of vitrinite is high in coal. The weathering of the outcrop is very serious. The abundance of organic matter in source rock reaches the poor to better rank. The major kerogens in mudstone are type-Ⅲ, type-Ⅱ2 and some type-Ⅱ1; the organic type of coal is type-Ⅲ. The thermal evolution of the source rocks in every structural unit is very different, from low-maturity to over-maturity. The depositional environment is reductive, which is good for the preservation of organic matter. The organic matter in source rocks is mainly from aquatic organisms and terrigenous input.     

Plant diversity as a good indicator of vegetation stability in a typical plateau wetland

Biotic indicators have been widely used to monitor wetland health. However, few studies have explicitly evaluated if plant diversity could serve as a useful community-level indicator of wetland stability, especially when wetlands are confronted with anthropogenic perturbations. Based on three-year record of wetland plant species abundance in Napahai plateau wetland, Shangri-la under the influence of varying anthropogenic perturbation types, our study tests the impact of such perturbations on plant richness and the relationship between ecosystem temporal stability and plant richness, and further assesses the effectiveness of using plant diversity indicator to probe ecosystem temporal stability of Napahai plateau wetland and the potential mechanisms. The results showed that anthropogenic perturbations could have contributed significantly to realistic variation in plant diversity, and further demonstrated that ecosystem temporal stability was positively related to realistic variation in plant diversity. In particular, communities with high levels of diversity might have better capacity to dampen perturbation impacts than communities with low levels of diversity, and statistical averaging could have played an important role in causing greater stability in more diverse communities. Also, asynchrony might have a stabilizing effect on community stability, and diversity could have stabilized communities through both species asynchrony and population stability propagation. Therefore, our results suggest that plant diversity could be used as a useful indicator of the stability conditions of plateau wetland ecosystems confronted with anthropogenic perturbations, and the preservation of plant communities at sufficient abundance and diversity is necessary for maintaining healthy plateau wetlands and for sustaining their essential ecosystem functions and services.     

Source-sink landscape spatial characteristics and effect on non-point source pollution in a small catchment of the Three Gorge Reservoir Region

The source and sink landscape patterns refer to landscape types or units that can either promote positive evolvement of non-point source (NPS) pollution process, or can prevent/defer the ecological process, respectively. Therefore, the role of a catchment landscape pattern in nutrient losses can be identified based on the spatial arrangement of source and sink landscapes. To reveal the relations between landscape spatial characteristics and NPS pollution in small catchment, a case study was carried out in a Wangjiagou small catchment of the Three Gorges Reservoir Region (TGRR), China. Google earth imagery for 2015 were processed and used to differentiate source and sink landscape types, and six subcatchments were selected as sample regions for monitoring nitrogen and phosphorus nutrients. Relative elevation, slope gradient and relative flow length was used to construct the Lorenz curves of different source and sink landscape types in the catchment, in order to assess the source and sink landscape spatial characteristics. By calculating the location-weighted landscape indices of each subcatchment and total catchment, the landscape spatial load characteristics affecting the NPS pollution was identified, with a further Pearson correlation analysis for location-weighted landscape indices and nitrogen-phosphorus monitoring indicators. The analysis of Lorenz curve has revealed that the obtained distribution trend of Lorenz curve and curve area quantified well the spatial characteristics of source and sink landscape pattern related to the relative elevation, slope gradient and relative flow length in small catchment. Results of Pearson correction analysis indicated that location-weighted landscape index (LWLI) combining of terrain and landscape type factor did better in reflecting the status of nitrogen and phosphorus loss than the indices related to relative elevation, slope gradient and relative flow length.     

直流电源故障的微机检测

介绍了利用IMB－PC 8088CPU的单板机控制检测多路直流电源过压、欠压。在出现此类故障时能发出报警声，并在显示器上显示故障电源编号，同时切断故障电源，启动同类备用电源，以便维修。     

CHARACTERISTICS OF PERIGLACIAL GEOMORPHOLOGY IN THE SOURCE AREA OF THE HUANGHE RIVER

There widely occur stretches of permafrost at more than 3,800-4,200 meters above sea level in the source area of the Huanghe (Yellow) River. The periglacial geomorphology develops quite well, including frozen disintegration geomorphology, freezing and thawing geomorphology in cold environments, periglacial dune, buried ices and fossil periglacial phenomena. In light of the relation between stratigraphy and periglacial phenomena, three periglacial periods can be divided, which are the Middle Pleistocene periglacial period, the Late Pleistocene periglacial period and modern periglacial period.     

CORRESPONDENCE ANALYSIS ON IMAGES OF JIANGXI PROVINCE AS A TOURIST DESTINATION

1IN T R O D U C T IO NAn image isa setofbelief,s ideasand impressionsthatapersonholdsaboutan object(KOTLER,1991).Similar-lydefinitionistheimage of a destinationas thesum ofbelief,sideasand impressionsthatpeoplehave ofa placeor destination(CROMPTON,1979;KO…     

不同温度下长茎葡萄蕨藻无机碳利用

【目的】阐明长茎葡萄蕨藻对无机碳的利用方式。【方法】运用pH漂移技术,探索藻体质量、藻体部位、照度和海水体积对长茎葡萄蕨藻(Caulerpa lentillifera)的pH补偿点的影响,并研究在3种不同温度(20、25、30℃)下,乙酰唑胺(AZ)、乙氧苯丙噻唑磺胺(EZ)、4,4′-二异硫氰基芪2,2′-二磺酸(DIDS)、钒酸盐(Van)4种抑制剂对长茎葡萄蕨藻无机碳的利用影响。【结果】藻体质量、藻体部位、照度和海水体积对长茎葡萄蕨藻的pH补偿点均无显著性影响(P>0.05),在一定范围内藻体质量或照度越大,达到pH补偿点时间越短,且pH补偿点均大于9。20℃下各抑制剂的抑制作用最大,其次为30℃,25℃最小,但3种温度下各抑制剂的抑制率无显著差别(P> 0.05)。4种抑制剂中DIDS对长茎葡萄蕨藻pH补偿点和无机碳利用均无显著影响(P> 0.05),剩余3种抑制剂的抑制作用依次为EZ>AZ>Van。【结论】藻体质量、藻体部位、照度和海水体积对长茎葡萄蕨藻pH补偿点无影响。温度对长茎葡萄蕨藻吸收利用无机碳没有明显影响。长茎葡萄蕨藻能利用HCO_3^-作为无机碳源进行光合作用。