共查询到20条相似文献,搜索用时 62 毫秒
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《高科技纤维与应用》2009,(5)
本发明属于计量测试技术及仪器领域,具体讲是涉及碳纤维复合管材电极电容式油位传感器及应用仪器。为提供碳纤维复合管材电极电容式油位传感器及应用仪器,该传感器及测量仪具有质量轻、结构更简单、制作工艺更简便的特点, 相似文献
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《高科技纤维与应用》2009,(5)
本发明提供了一种纳米碳纤维盘电极的制备方法,该方法先将直径不超过10μm的碳纤维用银胶与金属丝粘连后穿入毛细管,并从毛细管中穿出一截;再将穿出毛细管的碳纤维尖端用火焰蚀刻至纳米尺度;然后将碳纤维尖端撤回至毛 相似文献
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研究铜电极与碳纤维/环氧复合材料的粘接工艺,比较铜的表面处理方法对于粘接强度和导电性的影响,并验证粘接的可靠性.首先分别用机械打磨、化学表面处理、表面电镀方法对铜片进行处理,然后通过铜粉导电胶与碳纤维/环氧复合材料粘接,测试粘接强度及电阻率,再通过湿热老化实验,对粘接的可靠性进行比较.测试结果表明,经化学表面处理的铜片与碳纤维/环氧复合材料粘接强度达到1.34 MPa,老化后强度保留率为76%;电阻率为4.19 Ω*m,老化后电阻率增加率为4.8%.从而确定化学表面处理方法得到的粘接强度和导电效果较佳. 相似文献
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用聚乙烯醇缩丁醛将葡萄糖氧化酶(GOD)和纳米银固定于铂丝电极上并采用萘酚绿B作为电子媒介制得了新型葡萄糖酶生物传感器。实验发现,吸附在纳米银表面上的酶稳定且保持生物活性,电子媒介体与纳米银结合显著提高了该传感器的灵敏度。讨论了溶解性媒介体萘酚绿的浓度、溶液的pH值和温度对该电极电流响应的影响。该传感器在优化的实验条件下,对葡萄糖表现出良好的响应特性,如响应快、重现性和稳定性好,传感器线性范围为1.0×10-3~4.0×10-2mol/L,检出限为3.0×10-4mol/L。抗坏血酸、尿酸等对测定均无干扰。 相似文献
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Glucose-sensitive liposomes were prepared by incorporating hydrophobically modified glucose oxidase (EC 1.1.3.4.) into the
liposomal bilayer of dioleoylphosphatidylethanolamine and cholesteryl hemisuccinate. For the release test, calcein, a fluorescence
marker, was entrapped in the liposomes. The liposomes were stable under neutral conditions in terms of calcein release but
an extensive release was observed under acidic conditions. In the experiment of glucose concentration-dependent calcein release,
no release was observed for 180 min when the suspension of liposome was free of glucose. With a glucose concentration of 50 mg/dL,
no appreciable amount of calcein was released for the first 20 min, and then the release rate was accelerated. At 200 mg/dL
glucose concentration which is diagnostic and indicative for insulin-dependent diabetes, the lag time of calcein release became
shorter and a faster response was obtained. When glucose concentration further increased to 400 mg/dL, the calcein release
rate and the degree of release in 180 min were almost the same as the values when the glucose concentration was 200 mg/dL.
The glucose concentration-dependent release is due to pH change, since the suspension of liposomes became acidic during the
release experiments. 相似文献
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In this article we describe the preparation and characterization of a new glucose oxidase (GOD) biosensor. The GOD biosensor was immobilized onto pectin by using chromium III sulfate and electrode was coated with GOD containing pectin. Immobilization conditions were modified according to the results obtained by coated electrode. Optimum conditions were obtained when 10 U GOD and 1% pectin was reacted with 0.01 M chromium III sulfate. Developed biosensor is very accurate, fast responding, and stable. We could easily detect 1 mM glucose with a response time below 1 minute and could use our sensor up to 14 times (within 27 days) with 90% accuracy. 相似文献
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将纳米CdS∶Cu颗粒加入到葡萄糖酶(GOD)和辣根过氧化物酶(HRP)双酶膜中,与导电聚合物聚邻苯二胺(PoPD)经电化学聚合反应而固定此两酶,制备了电流型纳米CdS∶Cu颗粒双酶膜葡萄糖生物传感器,分析了CdS∶Cu纳米颗粒对传感器电流响应的影响,进行了传感器的性能测定。实验表明,引入CdS∶Cu纳米粒子和PoPD后可显著改进传感器响应性能,线性范围为0.55~9.2 mmol/L,检测下限为0.55 mmol/L,响应时间为20 s。稳定工作215天,传感器活性指标无显著变化,且抗干扰性强。 相似文献
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Qingye Han Weili Gong Zhenyu Zhang Lushan Wang Binglian Wang Lei Cai Qingjun Meng Yiwei Li Qingai Liu Yan Yang Lan Zheng Yaohong Ma 《International journal of molecular sciences》2021,22(11)
The discovery or engineering of fungus-derived FAD-dependent glucose 1-dehydrogenase (FAD-GDH) is especially important in the fabrication and performance of glucose biosensors. In this study, a novel FAD-GDH gene, phylogenetically distantly with other FAD-GDHs from Aspergillus species, was identified. Additionally, the wild-type GDH enzyme, and its fusion enzyme (GDH-NL-CBM2) with a carbohydrate binding module family 2 (CBM2) tag attached by a natural linker (NL), were successfully heterogeneously expressed. In addition, while the GDH was randomly immobilized on the electrode by conventional methods, the GDH-NL-CBM2 was orientationally immobilized on the nanocellulose-modified electrode by the CBM2 affinity adsorption tag through a simple one-step approach. A comparison of the performance of the two electrodes demonstrated that both electrodes responded linearly to glucose in the range of 0.12 to 40.7 mM with a coefficient of determination R2 > 0.999, but the sensitivity of immobilized GDH-NL-CBM2 (2.1362 × 10−2 A/(M*cm2)) was about 1-fold higher than that of GDH (1.2067 × 10−2 A/(M*cm2)). Moreover, a lower detection limit (51 µM), better reproducibility (<5%) and stability, and shorter response time (≈18 s) and activation time were observed for the GDH-NL-CBM2-modified electrode. This facile and easy immobilization approach used in the preparation of a GDH biosensor may open up new avenues in the development of high-performance amperometric biosensors. 相似文献
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考察了超声波均质及葡萄糖氧化酶(GOD)脱糖对鸡蛋清(CEW)粘度、蛋清中溶菌酶浓度和活力的影响. 结果表明,超声波均质和GOD脱糖均能有效降低蛋清的粘度,从而得到低粘度的均匀料液. 但随着超声波功率的增加和超声处理时间的延长,溶菌酶的浓度及活力呈下降趋势. 而经GOD脱糖处理后的料液溶菌酶活力随GOD加入量的增大而降低,浓度则先升高后下降. 将两种处理方法结合使用,料液粘度可以降至4.0 mPa×s,此时溶菌酶损失19.48%,酶活降低19.13%. 相似文献