共查询到18条相似文献,搜索用时 171 毫秒
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目的:研究以低含量猪去氧胆酸为原料,精制高含量猪去氧胆酸的最佳工艺。方法:采用正交设计L_9(3~4)方法,筛选出最佳除杂工艺,采用高效液相色谱法,测定猪去氧胆酸的含量,考察了水用量、pH、澄清剂的添加量,对猪去氧胆酸收率的影响,优选出合理除杂工艺;考察乙酸乙酯、活性炭用量和回流时间对猪去氧胆酸含量的影响,选出最佳精制工艺条件。结果:水量是10倍量、pH 11~12,澄清剂质量分数1.5%为最佳除杂工艺;乙酸乙酯用量为12倍量、活性炭的质量分数为3%,回流时间1 h为最佳的精制工艺。结论:采用正交设计筛选出的最佳精制工艺稳定可行,满足注射剂用药的要求。 相似文献
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Mice were fed a control diet or a diet supplemented with hyodeoxycholic acid, the most abundant bile acid contained in pig bile, for 4 weeks, after which their serum and livers were collected. The contents of total fatty acids of serum and liver cholesteryl esters, and of liver triglycerides, were reduced following the administration of the hyodeoxycholic acid‐supplemented diet, which was mainly due to the reductions in the contents of monounsaturated fatty acids. Free cholesterol contents in the serum and liver were not changed by hyodeoxycholic acid administration. Hyodeoxycholic acid administration reduced the gene expression levels of sterol regulatory element binding protein 1c, acetyl‐CoA carboxylase, fatty acid synthase, and stearoyl‐CoA desaturase‐1. Hyodeoxycholic acid administration markedly changes the ratio of FXR‐antagonist/FXR‐agonist bile acids in the enterohepatic tissues of the mice (1.13 and 7.60 in hyodeoxycholic acid and control diet groups, respectively). Our findings demonstrate that hyodeoxycholic acid administration exerts the hypolipidemic effect in mice, in which downregulations of de novo lipogenesis and desaturation of saturated fatty acids are suggested to play important roles. In addition, regulation of FXR activation through the selective modification of the enterohepatic bile acid pool may be involved in the hypolipidemic effect of hyodeoxycholic acid administration. 相似文献
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The effect of chenodeoxycholic acid, ursodeoxycholic acid and hyodeoxycholic acid on gallstone dissolution was studied in
the prairie dog. Cholesterol gallstones were found in all animals after feeding a semipurified diet plus 1.2% cholesterol
for six wk. Gallstone regression was examined by feeding a chow diet containing the bile acids (chenodeoxycholic acid, ursodeoxycholic
acid or hyodeoxycholic acid) alone (30 mg/kg/day) or in combination (chenodeoxycholic acid plus ursodeoxycholic acid) for
an additional six wk. Chenodeoxycholic acid was effective in dissolving established cholesterol gallstones (two out of 16
animals still had stones) and cholesterol crystals (six out of 16 animals had crystals); the hydrophilic bile acids, ursodeoxycholic
acid and hyodeoxycholic acid, were ineffective in the six-wk regression study. The lithogenic indices averaged 1.09 at the
end of the induction period: all biles became unsaturated with respect to cholesterol after the six-wk regression period (group
1, 0.82; group 2, 0.66; group 3, 0.81; group 4,0.84; group 5, 0.66). Cholesterol levels in liver, plasma and bile were elevated
after the six-wk induction phase (4.59 mg/g, 610 mg/dl and 0.36 mg/ml, respectively) but returned to near normal levels after
the six-wk regression period. Biliary bile acids contained increased levels of the dietary bile acid administered to each
group. This experiment shows that relatively hydrophobic bile acids may be more effective than hydrophilic bile acids for
gallstone dissolution during the period studied. 相似文献
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Mei Zhang Isabel M. Miranda Salvado Paula M. Vilarinho Armando J. D. Silvestre Artur M. S. Silva 《Journal of the American Ceramic Society》2007,90(2):358-363
Different macroscopic properties of PZT fibers have been obtained when using acetic acid and methacrylic acid to modify the PZT precursor. In order to clarify the role of the acids the molecular structure of the acidified PZT precursors was investigated and compared by gas chromatography-mass spectrometry, Fourier transform infrared, 13 C nuclear magnetic resonance (NMR) spectroscopy (solution and solid state 13 C NMR) and the reason for obtaining long PZT fibers is discussed. The results indicate that when methacrylic acid was used, long gel and ceramic fibers have been obtained because strongly co-ordinating carboxylate groups of methacrylic acid were formed. Linear chains, like those of methacrylic acid propyl ester and methacrylic acetate, have been formed in the PZT precursor sols. In addition, after heat treatment the polymer decomposed quickly so that pure perovskite could be obtained at low temperature in the PZT fibers. When acetic acid was used short fibers were obtained. Acetic acid may act as chelate agent to form oxo acetate in the precursors; this oxo acetate nature also resulted in PZT fibers drawing. However, the longest gel and ceramic fibers have been prepared from precursors with methacrylic acid. 相似文献
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Tissues of rats were examined for the presence of cholanoic acids. Quantitation of extraction, deconjugation, and isolation were verified by use of radioactive standards. Identification was made by thin layer and gas liquid chromatographic comparison to standards and mass spectrometry. All tissues examined were found to contain several conjugated cholanoic acids. Liver contained primarily cholic acid and peripheral tissues primarily dihydroxy compounds, mainly hyodeoxycholic acid. 相似文献
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Hsien-Ming Kao Tzu-Yi Shen Jyun-Da Wu Lung-Ping Lee 《Microporous and mesoporous materials》2008,110(2-3):461-471
Well-ordered cubic mesoporous silicas SBA-1 functionalized with thiol groups have been synthesized via co-condensation of tetraethoxysilane (TEOS) and 3-mercaptopropyltrimethoxysilane (MPTMS) templated by cetyltriethylammonium bromide (CTEABr) under strongly acidic conditions. Various synthesis parameters such as HCl concentration, synthesis temperature, and time for hydrothermal treatment were systematically investigated as a function of MPTMS contents. The materials thus obtained were characterized by a variety of techniques including powder X-ray diffraction (XRD), solid-state 13C and 29Si NMR spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and nitrogen sorption measurements. Direct evidence of the presence of chemically attached thiol moieties was provided by solid-state 29Si and 13C NMR spectroscopy. A high degree of structural ordering was still retained when MPTMS loading was incorporated up to 20 mol% in the synthesis mixture. Increasing the synthesis temperature and the time for the hydrothermal treatment not only increased the degree of framework cross-linking, but also did not lead to the undesirable phase transformation as often observed in the conventional synthesis of pure silica SBA-1. SEM results reveal that the morphology of thiol-functionalized SBA-1 depends on the HCl concentration used in the synthesis. The sample prepared with a low acid concentration exhibits a highly isotropic morphology with more facets than that of pure silica SBA-1, whereas it changes to a spherical shape as the acid concentration is increased. The maximum content of the attached thiol group (–SH) in the mesoporous framework is 2.39 mmol/g. The thiol-functionalized SBA-1 mesoporous materials are efficient Hg2+ adsorbents. 相似文献
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The effect of chitosan feeding (for 21 days) on intestinal bile acids was studied in male rats. Serum cholesterol levels in
rats fed a commercial diet low in cholesterol were decreased by chitosan supplementation. Chitosan inhibited the transformation
of cholesterol to coprostanol without causing a qualitative change in fecal excretion of these neutral sterols. Increased
fiber consumption did not increase fecal excretion of bile acids, but caused a marked change in fecal bile acid composition.
Litcholic acid increased sigificantly, deoxycholic acid increased to a leasser extent, whereas hyodeoxycholic acid and the
6β-isomer and 5-epimeric 3α-hydroxy-6-keto-cholanoic acid(s) decreased. The pH in the cecum and colon became elevated by chitosan
feeding which affected the conversion of primary bile acids to secondary bile acids in the large intestine. In the cecum,
chitosan feeding increased the concentration of α-,β-, and ω-muricholic acids, and lithocholic acid. However, the levels of
hyodeoxycholic acid and its 6β-isomer, of monohydroxy-monoketo-cholanoic acids, and of 3α, 6ξ, 7ξ-trihydroxy-cholanoic acid
decreased. The data suggest that chitosan feeding affects the metabolism of intestinal bile acids in rats. 相似文献