Studies on components of the contact phase system in patients with advanced gastrointestinal cancer.

The authors have studied components of the contact system in plasma obtained from patients with advanced gastrointestinal cancer. Plasma samples from 118 healthy blood donors served as controls. Plasma prekallikrein (PKK) values, evaluated by chromogenic peptide substrate technique, were significantly decreased in patients with cancer compared with healthy blood donors. High molecular weight kininogen (HMwK) and Hageman factor (FXII) values, assayed by immunochemical techniques, were also decreased in the patients with cancer. The changes of contact factors were most pronounced in patients with liver metastasis. The most striking observation in our study, however, was the elevated inhibitor values in patients with cancer. Alpha-2-macroglobulin (alpha 2-M) and C1 inhibitor (C1INH) values, determined both by functional and immunochemical techniques, were markedly increased in patients with cancer. In conclusion, this study shows that patients with intestinal cancer have reduced values of contact factors and markedly elevated inhibitor values which indicate that development of malignant tumors in the gastrointestinal tract is associated with changes in the contact system of plasma.     

他莫昔芬对人胰腺癌细胞增殖的影响及其分子机制

目的:探讨他莫昔芬(TAM)对人胰腺癌细胞增殖的影响及其机制。方法:采用钙离子荧光指示剂flour3/Am,流式细胞仪动态检测TAM作用后人胰腺癌细胞(CapanⅡ)内游离钙离子([Ca2+]i)水平的变化,同时用MTT比色法测定细胞增殖情况。结果:TAM使细胞内[Ca2+]i水平上升,与静息状态细胞内[Ca2+]i水平相比差异有统计学意义,P<005。高浓度的TAM对癌细胞的增殖有抑制作用。结论:TAM有抑制人胰腺癌细胞增殖的作用,其作用机制除通过竞争性结合雌激素受体来抑制雌激素的作用外,还可通过诱导细胞内[Ca2+]i水平升高,抑制Ca2+依赖蛋白激酶C和钙调蛋白,从而抑制癌细胞的生长。     

Studies on the effect of lentinan on human immune system. II. In vivo effect on NK activity, MLR induced killer activity and PHA induced blastic response of lymphocytes in cancer patients

Immunological activities of lymphocytes were studied in patients with cancer of digestive organs and mamma after administration of lentinan. When 11 cancer patients received lentinan i.v. 2 mg, NK activity of lymphocytes was increased in 4 patients on the following day. With the same condition, MLR induced killer activity against Raji cell of lymphocytes was increased in 3/7 patients and PHA response was also increased in 3/7 patients. These activities were returned to values before the administration of lentinan. Then each activity was examined after administration of lentinan twice a week. NK activity, MLR induced killer activity and PHA response were increased in 4/15, 8/12 and 5/11 patients, respectively.     

TruScreen系统在宫颈癌筛查中的应用效果

目的：探讨宫颈癌筛查系统TruScreen（TS）在宫颈癌筛查中的应用效果。方法回顾性分析368例检查者,分为TS检测组和TCT检测组,每组184例,TS检测组行TS检测,TCT检测组行液基薄层细胞学（TCT）检测,以组织病理学为诊断标准,比较TS与TCT检查在宫颈癌筛查中的应用效果。结果 TS检测组与TCT检测组检测敏感度分别为93.2%、100%,特异度分别为76.0%、75.0%,阳性符合率分别为64.7%、55.7%。结论 TS检测与TCT检测在宫颈癌筛查中具有相似的检测效果,值得临床推广和运用。     

基因-病毒治疗系统CNHK200-hA对肺癌治疗的研究

目的　构建抗肿瘤新生血管生成的基因治疗与病毒治疗相结合的基因 病毒治疗系统CNHK2 0 0 hA ,并对其肺癌的体内外治疗作用进行初步研究。方法　通过Westernblot分析 ,观察携带抗肿瘤新生血管生成基因angiostatink1 5 (hA)的基因 病毒治疗系统CNHK2 0 0 hA和非增殖型腺病毒对介导k1 5蛋白表达的影响 ;通过细胞病理作用及动物试验 ,比较CNHK2 0 0 hA和非增殖型腺病毒对人肺癌细胞株A5 4 9的杀伤作用及对肺癌裸鼠移植瘤的治疗作用。结果　基因 病毒治疗系统CNHK2 0 0 hA能够介导hA基因在肺癌细胞内高效表达k1 5蛋白 ,其表达量高于非增殖型腺病毒Ad hA。细胞病理效应显示 ,CNHK2 0 0 hA对A5 4 9的杀伤作用明显优于Ad hA ,CNHK2 0 0 hA在感染复数 (MOI)值为 1时可完全杀伤A5 4 9细胞 ,而达到相同杀伤效应的Ad hA的MOI值为 1 0 0。动物试验显示 ,CNHK2 0 0 hA对肺癌的疗效明显好于Ad hA及肿瘤增殖型病毒ONYX 0 1 5。结论　插入了抗肿瘤新生血管生成基因hA的基因 病毒治疗系统CNHK2 0 0 hA ,可明显提高hA基因的表达量 ,其在体内外的抗肺癌作用较非增殖型腺病毒Ad hA及肿瘤增殖型病毒ONYX 0 1 5进一步提高。     

Treatment of patients with disseminated colorectal cancer with recombinant human alpha 2-interferon. Studies on the immune system

The influence of recombinant human alpha 2-interferon (alpha 2-IFN) therapy on various aspects of the immune system was studied in 18 patients with disseminated colorectal cancer. The IFN was given as continuous (20 X 10(6) units/m2 three times weekly) or intermittent (50 X 10(6) units/m2 daily for 5 consecutive days every 4 weeks) treatment. Natural killer (NK) cell activity increased during continuous treatment and in the patients receiving repeated cycles of IFN, all cycles seemed to be associated with an elevation of NK activity. Prior to treatment, addition of IFN to the assay in vitro induced an enhancement of NK activity, whereas during treatment, IFN in vitro did not cause any further enhancement of NK activity. The proportions of total T cells, suppressor T cells and helper T cells, as measured by Leu 1, Leu 2a and Leu 3a monoclonal antibodies, were not altered to any major extent during treatment. This was found to be the case also for the number of cells detected by monoclonal antibodies against NK cells (Leu 7). The phagocytic activity of granulocytes was not altered during IFN therapy, whereas the capacity of these cells to reduce nitroblue tetrazolium (NBT) increased after the first injection of IFN. The in vivo influence of high doses of highly purified recombinant alpha 2-IFN on NK cells and granulocytes seems to be similar to that of partially purified natural IFN-alpha.