新型纳米纤维支架的细胞生物相容性

背景：高孔隙率聚己内酯纳米纤维支架具有适合血管平滑肌细胞黏附、增殖的多级孔径结构,具有良好的细胞生物相容性。目的：探讨高孔隙率聚己内酯静电纺丝纳米纤维支架的细胞相容性。方法：根据支架的制作工艺不同分为传统支架组、新型纳米纤维支架组两组,另设单纯细胞组为对照组。采用组织块贴壁法体外原代培养兔主动脉平滑肌细胞并进行传代,用3~6代细胞作为实验用种子细胞。应用WST-1法测定平滑肌细胞黏附率、增殖力,光镜及扫描电镜观察细胞形态,评估支架的细胞生物相容性。结果与结论：高孔隙率聚己内酯纳米纤维支架对细胞形态无明显影响,新型支架上的种子细胞黏附、增殖及代谢活性情况较传统支架好。提示,高孔隙率聚己内酯静电纺丝纳米纤维支架具有较高的细胞相容性。     

纳米羟基磷灰石/聚己内酯复合大鼠骨髓间充质干细胞的生物相容性

背景:纳米羟基磷灰石/聚己内酯是一种具有优良生物相容性和生物活性的典型生物复合材料.目的:分析纳米羟基磷灰石/聚己内酯电纺薄膜作为组织工程骨支架的可行性.方法:采用静电纺丝技术制备纳米羟基磷灰石/聚己内酯电纺薄膜,将其与第3代 SD 大鼠骨髓间充质干细胞复合培养,在地塞米松、β-磷酸甘油钠、维生素C成骨诱导剂诱导下,诱导骨髓间充质干细胞向成骨细胞转化.结果与结论:纳米羟基磷灰石/聚己内酯支架具有合适的微孔结构,且孔道相互贯通.①倒置显微镜观察:复合培养7 d后细胞大部分为梭形,细胞开始分裂;14 d后,细胞生长比较旺盛,数量明显增多,细胞分泌基质并黏附于支架上.②扫描电镜观察:复合培养7 d后大量细胞位于支架孔隙内生长,增殖良好,细胞大多呈梭形,双极突起,形态较佳,呈立体状生长,并分泌基质,有纤维连接蛋白生成.表明纳米羟基磷灰石/聚己内酯支架具有良好的生物相容性,是骨组织工程的良好载体.     

两种聚（左旋乳酸-己内酯）静电纺纳米纤维膜的性能比较

背景:聚左旋乳酸和聚己内酯各自都有其优点与缺点,而共聚或共混后性能可以得到有效的改善,但因为两者添加比例的不同会对性能有一定的影响,在不吲的纺丝溶液浓度下纺出的纤维性能亦会有所差异.目的:通过对两种原料聚(左旋乳酸-己内酯)(75/25;50/50)在不同纺丝液浓度下制得的纳米纤维膜各种性能的比较,选出最佳的原料和相应的纺丝液浓度.设计、时间及地点:对比观察实验,于2007-09/2008-11在东华大学生物材料与组织工程实验室完成.材料:将聚聚(左旋乳酸-己内酯)材料在乳酸/己内酯为75/25和50/50两种比例下,在质量分数为4%,6%,8%和10%纺丝液浓度下通过静电纺丝制备纳米纤维膜.方法:扫描电镜样品经表面喷金后在10 kV加速电压下观察纤维膜的彤貌.在万能材料测试机测试其断裂强度和断裂伸长率.采用MTT法测试猪髋动脉内皮细胞在纳米纤维膜上的黏附与增殖情况.主要观察指标:静电纺纳米纤维膜的纤维形态、力学性能及生物相容性.结果:通过扫描电镜观察发现由质量分数为6%聚(左旋乳酸-己内酯)(50/50)制备的纤维膜具有更好的纤维形态,且直径分布均匀;拉伸力学测试显示由聚(左旋乳酸-己内酯)(50/50)制备的纤维膜比聚(左旋乳酸-己内酯)(75/25)具有更高的断裂伸长率,但断裂应力较低;细胞生物相容性实验表明猪髋动脉内皮细胞在质鼍分数为6%和8%聚(左旋乳酸-己内酯)(50/50)的纳米纤维膜上更能有效的黏附与增殖.结论:纺丝液质量分数为6%的聚(左旋乳酸-己内酯)(50/50)制得的纳米纤维膜各项性能较优.     

聚(左旋乳酸-己内酯)/Fe304取向超细纤维的制备及生物相容性

背景:在静电纺过程中利用磁场效应的方法更适合制备载有磁性纳米粒子的取向纤维.但当前的研究多限于水溶性聚合物材料的电纺.目的:通过相转移方法制备均匀分散有Fe3O4磁性纳米粒子的聚(左旋乳酸-己内酯)溶液,在外加磁场作用下通过静电纺技术制备聚(左旋乳酸-己内酯)/Fe3O4定向排列复合纤维,体外接种猪髋动脉内皮细胞,初步观察细胞相容性.设计、时间及地点:对比观察实验,于2008-0612008-10在东华大学化学化上与生物工程学院生物科学研究所生物材料与组织T程实验室完成.材料:聚(左旋乳酸-己内酯)无规共聚物(50:50,M.30-40万)由日本Nara Medical University提供;猪髋动脉内皮细胞由中国科学院细胞所提供.方法:采用相转移法将水相中的Fe3O4磁性纳米粒子转移至有机溶剂中,制备聚(左旋乳酸-己内酯),Fe3O4的溶液.利用磁场对Fe3O4磁性纳米粒子的牵引作用,静电纺制备取向超细纤维.主要观察指标:通过扫描电镜对纤维进行表面形态、取向度进行分析,采用透射电镜分析Fe3O4磁性纳米粒子在纤维中分散和分布情况.在复合Fe3O4磁性纳米粒了的聚(左旋乳酸-己内酯)静电纺纤维膜上体外接种猪髋动脉内皮细胞,采用MTT法检测纤维膜上细胞的黏附和增殖能力以评价其生物相容性.结果:通过添加乳化剂汕酸钠成功得到分散均匀的Fe3O4磁性纳米粒子三氯甲烷溶剂.扫描电镜结果表明在磁场的影响下,静电纺得到的纤维农面光滑、无明显珠状物分布:排列方向沿磁场磁力线分布.取向度良好.透射电镜结果显示Fe3O4磁性纳米粒子在静电纺超细纤维中分散度良好.细胞生物相容性结果显示制备的载有磁性纳米粒子的聚(左旋乳酸-己内酯)静电纺超细纤维细胞黏附率优于纯聚(左旋乳酸-己内酯)纤维;其细胞增殖率与纯聚(左旋乳酸-己内酯)静电纺超细纤维接近.结论:含有一定浓度的Fe3O4磁性纳米粒子的聚(左旋乳酸-己内酯)溶液,在外加磁场的作用下,静电纺得到的纤维排列方向沿磁场磁力线分布,取向度极佳,且具有良好的细胞相容性.     

静电纺壳聚糖/胶原蛋白复合纳米纤维的细胞相容性

目的:静电纺是一种使带电荷的聚合物溶液或熔体在静电场中射流来制备聚合物纳米级纤维的加工方法,采用此种技术制备壳聚糖/胶原蛋白复合纳米纤维,并观察其细胞相容性.方法:实验于2006-11/2007-10在东华大学化学化工与生物工程学院生物材料与组织工程实验室完成.①支架材料制备:以六氟异丙醇/三氟乙酸为溶剂体系,采用静电纺制备复合纳米纤维,其中壳聚糖/胶原蛋白的质量比分别为100:0,80:20,50:50,20:80与0:100.②细胞相容性观察:体外接种猪髋动脉内皮细胞,苏木精-伊红染色法观察细胞形态,MTT法检测细胞黏附和增殖情况.结果:猪髋动脉内皮细胞在壳聚糖/胶原蛋白复合纤维表面贴附牢固,外形饱满,多呈长梭形,具有良好的生长形态;MTT法结果显示纳米纤维能够有效地促进内皮细胞在材料表面的黏附和增殖,质量比为20:80材料组细胞黏附、增殖能力最强,其次为50:50组.结论: 静电纺壳聚糖/胶原蛋白复合纳米纤维具有良好的细胞相容性,可望成为一种新型的组织工程支架材料.     

静电纺丝聚乳酸复合物纳米纤维材料与小鼠神经干细胞的生物相容性

背景：聚乳酸聚乙醇酸支架材料广泛应用于组织工程学领域,但其细胞黏附性较差、缺乏活性功能基团以及疏水性较强等缺点限制了其进一步的发展和应用。目的：观察小鼠神经干细胞与静电纺丝聚乳酸聚乙醇酸/聚乙二醇共聚物纳米纤维支架材料的体外相容性。方法：自孕15 d CD-1小鼠胚胎大脑皮质分离培养小鼠神经干细胞。静电纺丝法制备聚乳酸聚乙醇酸和聚乳酸聚乙醇酸/聚乙二醇纳米纤维支架材料,扫描电镜观察材料结构;取第5代神经干细胞分别接种于聚乳酸聚乙醇酸和静电纺丝聚乳酸聚乙醇酸/聚乙二醇纳米纤维支架材料上,进行体外培养。结果与结论：扫描电镜检测显示,两种支架材料呈现相互交联的多孔网状结构。聚乳酸聚乙醇酸组和静电纺丝聚乳酸聚乙醇酸/聚乙二醇组纤维直径和孔隙率差异无显著性意义（P 〉0.05）。CCK-8检测显示,两种材料无明显细胞毒性。神经干细胞在支架材料中生长良好,两组吸光度值均随培养时间延长而增大,两组在培养1,3,5,7,9,11 d吸光度值差异均有显著性意义（P 〈0.05）。两组材料培养3,6,9 h,静电纺丝聚乳酸聚乙醇酸/聚乙二醇组的细胞黏附率明显高于聚乳酸聚乙醇酸组（P 〈0.05）。Hoechst染色显示两组细胞核质均染,形态正常,静电纺丝聚乳酸聚乙醇酸/聚乙二醇组细胞数量明显多于聚乳酸聚乙醇酸组（P 〈0.05）。扫描电镜观察显示,与聚乳酸聚乙醇酸组相比,静电纺丝聚乳酸聚乙醇酸/聚乙二醇组神经干细胞在支架上的生长情况和基质分泌更好。结果说明,静电纺丝法制备的静电纺丝聚乳酸聚乙醇酸/聚乙二醇纳米纤维支架细胞生物相容性良好,安全无毒,具备合适的孔径和孔隙率,适宜神经干细胞生长,是一种适用于组织工程优质的支架载体。     

聚（左旋乳酸-己内酯）／Fe3O4取向超细纤维的制备及生物相容性

背景：在静电纺过程中利用磁场效应的方法更适合制备载有磁性纳米粒子的取向纤维。但当前的研究多限于水溶性聚合物材料的电纺。目的：通过相转移方法制备均匀分散有Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）溶液,在外加磁场作用下通过静电纺技术制备聚（左旋乳酸-己内酯）／Fe3O4定向排列复合纤维,体外接种猪髋动脉内皮细胞,初步观察细胞相容性。设计、时间及地点：对比观察实验,于2008—06／2008—10在东华大学化学化工与生物工程学院生物科学研究所生物材料与组织工程实验室完成。材料：聚（左旋乳酸己内酯）无规共聚物（50：50,Mw30-40万）由日本Nara Medical University提供;猪髋动脉内皮细胞由中国科学院细胞所提供。方法：采用相转移法将水相中的Fe3O4磁性纳米粒子转移至有机溶剂中,制备聚（左旋乳酸己内酯）,Fe3O4的溶液,利用磁场对Fe3O4磁性纳米粒子的牵引作用,静电纺制备取向超细纤维。主要观察指标：通过扫描电镜对纤维进行表面形态、取向度进行分析,采用透射电镜分析Fe3O4磁性纳米粒子在纤维中分散和分布情况。在复合Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）静电纺纤维膜上体外接种猪髋动脉内皮细胞,采用MTT法检测纤维膜上细胞的黏附和增殖能力以评价其生物相容性。结果：通过添加乳化剂油酸钠成功得到分散均匀的Fe3O4磁性纳米粒子三氯甲烷溶剂。扫描电镜结果表明在磁场的影响下,静电纺得到的纤维表面光滑、无明显珠状物分布;排列方向沿磁场磁力线分布,取向度良好。透射电镜结果显示Fe3O4磁性纳米粒子在静电纺超细纤维中分散度良好。细胞生物相容性结果显示制备的载有磁性纳米粒子的聚（左旋乳酸-己内酯）静电纺超细纤维细胞黏附率优于纯聚（左旋乳酸-己内酯）纤维;其细胞增殖率与纯聚（左旋乳酸-己内酯）静电纺超细纤维接近。结论：含有一定浓度的Fe3O4磁性纳米粒子的聚（左旋乳酸-己内酯）溶液,在外加磁场的作用下,静电纺得到的纤维排列方向沿磁场磁力线分布,取向度极佳,且具有良好的细胞相容性。     

聚左旋乳酸/壳聚糖电纺丝纳米纤维支架与兔内皮祖细胞的生物相容性

背景：电纺丝技术能够使许多高分子材料制备出与细胞外基质相似的三维纳米纤维支架。聚乳酸/壳聚糖纳米纤维复合支架材料能够克服材料的不足,提高组织工程支架生物相容性。目的：评价聚左旋乳酸/壳聚糖电纺丝纳米纤维支架与兔内皮祖细胞的生物相容性。方法：电纺丝技术制备聚左旋乳酸,壳聚糖,聚左旋乳酸/壳聚糖的纳米纤维支架,扫描电镜观察其形貌结构。纳米纤维支架与内皮祖细胞进行复合培养后,观察细胞在不同材料上的黏附率、一氧化氮分泌,生长特征和在聚左旋乳酸/壳聚糖纳米纤维支架上的细胞表型特征。结果与结论：聚左旋乳酸/壳聚糖纳米纤维支架比聚左旋乳酸、壳聚糖具有更合适的纤维直径,具有与细胞外基质相似的纳米纤维三维多孔结构。聚左旋乳酸/壳聚糖纳米纤维支架能够促进内皮祖细胞黏附率和细胞的一氧化氮分泌（P〈0.05,P〈0.01）。内皮祖细胞能够在聚左旋乳酸/壳聚糖复合材料膜上融合成片,保持了细胞的完整形态和分化功能,显示了内皮细胞特异性的vWF表型。提示聚左旋乳酸/壳聚糖电纺丝纳米纤维支架与兔内皮祖细胞具有良好的生物相容性。     

静电纺丝纳米纤维膜作为骨骼肌组织工程支架材料的细胞相容性

背景:有报道以生物可降解的胶原盘或聚L-乳酸、聚羟基乙酸、聚L-乳酸/聚羟基乙酸共聚物等作为骨骼肌组织工程的支架材料,各有优缺点,不能完全满足骨骼肌组织工程的需要。目的:探讨静电纺丝纳米纤维膜作为骨骼肌组织工程支架材料的可行性。方法:制备7种不同组分的静电纺丝纳米纤维膜,以其浸提液为培养基培养第3代SD乳鼠成肌细胞,以含体积分数20%新生小牛血清的F12培养基培养的为对照。采用MTT法和扫描电镜检测成肌细胞在各组材料的黏附及生长情况。结果与结论:各组分静电纺丝纳米纤维膜吸光度值与对照组间差异无显著性意义(P>0.05)。各组分静电纺丝纳米纤维膜组成肌细胞黏附率差异有显著性意义(P<0.05)。扫描电镜与上述结果一致。含70%聚乳酸+20%蚕丝蛋白+10%胶原组成电纺丝纳米纤维膜组可见大量成肌细胞黏附,呈梭形,两极伸展,排列规律,效果最好。其他各组细胞少,形态不规则,似衰退期成肌细胞。提示静电纺丝纳米纤维膜无细胞毒性,对成肌细胞的增殖无影响,成肌细胞能良好地黏附;以70%聚乳酸+20%蚕丝蛋白+10%胶原组分效果最佳。     

不同组分电纺丝素蛋白/聚己内酯超细纤维膜与小鼠成纤维细胞的相容性

背景:近年来,静电纺丝法已被认为是一种制各纳米至亚微米级纤维组织工程支架的简便方法.目的:对3种由静电纺丝法制备的纤维膜进行生物学评价.设计、时间及地点:观察性实验,于2009 01/04在杭州师范大学临床医学院完成.材料:电纺丝素蛋白/聚己内酯超细纤维膜(SF70/PCL30,SF50/PCL50)以及丝素蛋白/聚己内酯/纳米羟基磷灰石超细纤维膜(SF50/PCL50-nHA,其中纳米羟基磷灰石含量为30%)由浙江理工大学省部共建"先进纺织材料与制备技术教育部重点实验室"制备.方法:将L929细胞以5.5×108L-1浓度接种在3种电纺超细纤维膜上进行培养.在1,3,5 h和1,4,7d时用MTT法测定细胞黏附和增殖情况,在1 d和7 d时扫描电镜观察细胞的形态.主要观察指标:L929细胞在电纺SF70/PCL30,SF50/PCL50,SF50/PCL50-nHA超细纤维膜上的黏附和增殖情况;L929细胞的形态特征.结果:相比于没有加入纳米羟基磷灰石的电纺纤维膜,电纺SF50/PCL50-nHA 超细纤维膜会较好地提高细胞在其上的黏附和增殖能力.扫描电镜观察也表明,L929细胞可以在电纺SF50/PCL5-nHA 超细纤维膜上很好地呈梭形生长,并且在其表面可以观察到丰富的绒毛和伪足,伪足与材料紧密相联.结论:3种电纺超细纤维膜特别是电纺SF50/PCL50-nHA 超细纤维膜可以很好地应用于组织再生.     

Toward improved wound dressings: effects of polydopamine-decorated poly(lactic-co-glycolic acid) electrospinning incorporating basic fibroblast growth factor and ponericin G1

Artificial dressings composed of degradable polymer materials have a wide range of applications in skin repair. The structure and properties, in particular, the antibacterial properties, of the material surface are crucial for biological processes such as cell adhesion, proliferation, and skin regeneration. In this study, we aimed to prepare poly(lactic-co-glycolic acid) (PLGA) nanofiber scaffolds modified by polydopamine using electrospinning technology in order to produce polydopamine-modified degradable PLGA nanocomposites. The polydopamine-PLGA scaffold was endowed with excellent protein adhesion ability through the cross-linking of two biologically active factors, basic fibroblast growth factor (bFGF) and ponericin G1, significantly improving skin repair ability. The electrospun nanofiber scaffold was shown to have a structure similar to that of the natural cell matrix and created a more favorable microenvironment for cell growth. Surface modification by polydopamine dramatically improved the hydrophilicity of the nanofiber scaffold, increasing its ability to absorb active factors and its biocompatibility. The bFGF and ponericin G1 loaded onto the scaffold surface (PDA-PLGA/bFGF/ponericin G1 nanofiber scaffold) strongly promoted the antibacterial and cell proliferation-promoting properties and greatly enhanced the adhesion and proliferation of cells on the scaffold surface. The nanofiber scaffold also promoted wound healing and tissue collagen production in a rat wound healing model. Together, these findings indicate that the polydopamine-PLGA/bFGF/ponericin G1 nanofiber scaffold exhibits good biocompatibility and antibacterial properties, suggesting that it possesses potential value for skin tissue regeneration applications.

Artificial dressings composed of degradable polymer materials have a wide range of applications in skin repair.     

脱细胞异体真皮基质与人脂肪干细胞的生物相容性

背景:脱细胞异体真皮基质具有优良的生物相容性和组织细胞诱导功能.目的:评价人脂肪干细胞与脱细胞异体真皮基质的生物相容性.方法:取健康成年人吸脂术后的脂肪组织分离脂肪干细胞,并行原代与传代培养,传至第3 代,将细胞与脱细胞异体真皮基质联合体外培养3,7 d,倒置相差显微镜和扫描电镜观察细胞在支架材料上的黏附、生长及增殖情况,并计算细胞在材料上的黏附率;XTT比色法检测细胞的生长增殖情况.结果与结论:脂肪干细胞在支架材料上分布均匀,24 h内细胞开始伸展、黏附,二三天完全伸展变形,以梭形为主,呈网状排列;随着培养时间延长,支架上的细胞逐渐增多;人脂肪干细胞细胞与脱细胞异体真皮基质混合培养后平均黏附率为95.03%,并保持正常的生长增殖速度,表明支架对细胞具有良好的黏附性;脱细胞异体真皮基质材料与人脂肪干细胞复合后相容性良好.     

运动性关节软骨缺损支架材料的选择及其生物相容性

背景：骨软骨支架是用于承载细胞,供细胞黏附、生长、增殖、分化的载体。目的：总结运动性关节软骨缺损支架材料的应用进展及其生物替代材料的生物相容性。方法：以＂关节软骨,生物材料,工程软骨,支架材料,生物相容性＂为中文关键词,以＂tissue enginneering,articular cartilage,scaffold material＂为英文关键词,采用计算机检索维普数据库、PubMed数据库1993-01/2010-11相关文章。纳入与有关修复关节软骨损伤、生物材料、支架材料、生物相容性等相关的文章。以20篇文献为重点对运动性关节软骨缺损修复用的生物材料的生物相容性进行了讨论。结果与结论：天然软骨支架材料因其具有细胞识别信号,故生物相容性好,细胞黏附率高,但力学性能较差。有些人工合成材料生物相容性不理想、亲水性差、对细胞吸附不足,人工合成高分子聚合物生物相容性良好。复合支架利用不同生物材料的优点克制材料的局限性制备理想的复合支架,其混合比例、混合技术还有待进一步研究。目前尚无一种材料完全满足组织工程的要,通过材料制备技术的改进或将几种不同材料的复合,材料的性能会不断的提高。     

Characterization and osteogenic evaluation of mesoporous magnesium–calcium silicate/polycaprolactone/polybutylene succinate composite scaffolds fabricated by rapid prototyping

The properties of scaffolds for bone tissue engineering, including their biocompatibility, highly interconnected porosity, and mechanical integrity, are critical for promoting cell adhesion, proliferation, and osteoinduction. We used various physical and biological assays to obtain in vitro confirmation that the proposed composite scaffolds are potentially suitable for applications to bone tissue engineering. The proposed new composite scaffolds, which we fabricated by a rapid prototyping technique, were composed of mesoporous magnesium–calcium silicate (m_MCS), polycaprolactone (PCL), and polybutylene succinate (PBSu). We systematically evaluated the characteristics of the composite scaffolds, such as the hydrophilicity and bioactivity. We also investigated the proliferation and osteogenic differentiation of human mesenchymal stem cells (MSCs) scaffolded on the m_MCS/PCL/PBSu composite. Our results showed that, compared to the m_MCS/PCL scaffold, the m_MCS/PCL/PBSu scaffold has improved water absorption, in vitro degradability, biocompatibility, and bioactivity in simulated body fluid, while its mechanical strength is reduced. Moreover, the results of the cytotoxicity tests specified in ISO 10993-12 and ISO 10993-5 clearly indicate that the m_MCS/PCL scaffold is not toxic to cells. In addition, we obtained significant increases in initial cell attachment and improvements to the osteogenic MSC differentiation by replacing the m_MCS/PCL scaffold with the m_MCS/PCL/PBSu scaffold. Our results indicate that the m_MCS/PCL/PBSu scaffold achieves enhanced bioactivity, degradability, cytocompatibility, and osteogenesis. As such, this scaffold is a potentially promising candidate for use in stem cell-based bone tissue engineering.

A new composite scaffold consisting of mesoporous magnesium–calcium silicate (m_MCS), polycaprolactone (PCL), and polybutylene succinate (PBSu) was manufactured by a rapid prototyping technique, for stem cell-based bone tissue engineering.     

骨髓基质细胞与聚己内酯的生物相容性

背景:骨缺损修复一直是骨科领域的难题之一,近年来,组织工程化骨组织的研究为骨缺损修复提供了全新的思路和方法,而生物材料的生物相容性检测是其中重要的一个环节。目的:探讨骨髓基质细胞与聚己内酯的生物相容性。设计:对照观察。单位:新疆医科大学第一附属医院骨科。材料:实验于新疆医科大学第一附属医院骨科实验室完成。选4～8周龄健康新西兰大白兔,体质量2kg左右。方法:①抽吸双侧股骨骨髓并混入RPMI1640完全培养基进行细胞培养,然后进入传代培养。②进行细胞接种并分为聚己内酯组及对照组,对照组单纯接种细胞,聚己内酯组将骨髓基质细胞与聚己内酯体外复合培养,进行形态学观察、细胞增殖、蛋白质含量及酶学测定。主要观察指标:骨髓基质细胞生长及与聚乙内酯生物材料附着情况。结果:对照组细胞多向梭形细胞或多角形细胞转化。聚己内酯组骨髓基质细胞能在聚己内酯上贴附、繁殖,其生长及功能不受影响,并且聚己内酯具有一定的促细胞增殖作用。结论:聚己内酯具有良好的细胞相容性,有可能作为骨髓基质细胞的载体应用于组织工程的研究。     

A novel therapy strategy for bile duct repair using tissue engineering technique: PCL/PLGA bilayered scaffold with hMSCs

The current clinical treatments for complications caused by hepatobiliary surgery still have some inevitable weakness. The aim of the study was to fabricate a tissue‐engineered bile duct that utilized a novel bilayered polymer scaffold combined with human bone marrow‐derived mesenchymal stem cells (hMSCs) for new treatment of biliary disease. The biocompatibility of polycaprolactone (PCL) (PCL)/poly(lactide‐co‐glycolide) (PLGA) scaffold with hMSCs was first examined, and the hMSC–PCL/PLGA constructs (MPPCs) prepared. The MPPCs and blank scaffolds were then transplanted into 18 pigs for evaluation its efficacy on bile duct repairing, respectively. In vitro, the PCL/PLGA scaffold was verified to support the adhesion, proliferation and matrix deposition of hMSCs. There was no sign of bile duct narrowing and cholestasis in all experimental animals. At 6 months, the MPPCs had a superior repairing effect on the bile duct injury, compared with the blank PCL/PLGA scaffolds. Therefore, the implanted scaffolds could not only support the biliary tract and allow free bile flow but also had direct or indirect positive effects on repair of injured bile duct. Copyright © 2015 John Wiley & Sons, Ltd.     

Coaxial nanofiber scaffold with super-active platelet lysate to accelerate the repair of bone defects

To develop biocomposite materials with the local sustained-release function of biological factors to promote bone defect repair, coaxial electrospinning technology was performed to prepare a coaxial nanofiber scaffold with super-active platelet lysate (sPL), containing gelatin/PCL/PLLA. The nanofibers exhibited a uniform bead-free round morphology, observed by a scanning electron microscope (SEM), and the core/shell structure was confirmed by a transmission electron microscope (TEM). A mixture of polycaprolactone and sPL encapsulated by hydrophilic gelatin and hydrophobic l-polylactic acid can continuously release bioactive factors for up to 40 days. Encapsulation of sPL resulted in enhanced cell adhesion and proliferation, and sPL loading can increase the osteogenesis of osteoblasts. Besides, in vivo studies demonstrated that sPL-loaded biocomposites promoted the repair of skull defects in rats. Therefore, these results indicate that core–shell nanofibers loaded with sPL can add enormous potential to the clinical application of this scaffold in bone tissue engineering.

Coaxial electrospinning three-dimensional scaffold and its release various biological factors after filling the bone defect to induce adhesion and proliferation of osteoblasts on the nano scaffold.     

Characterization of a synthetic bioactive polymer by nonlinear optical microscopy

Tissue Engineering is a new emerging field that offers many possibilities to produce three-dimensional and functional tissues like ligaments or scaffolds. The biocompatibility of these materials is crucial in tissue engineering, since they should be integrated in situ and should induce a good cell adhesion and proliferation. One of the most promising materials used for tissue engineering are polyesters such as Poly-ε-caprolactone (PCL), which is used in this work. In our case, the bio-integration is reached by grafting a bioactive polymer (pNaSS) on a PCL surface. Using nonlinear microscopy, PCL structure is visualized by SHG and proteins and cells by two-photon excitation autofluorescence generation. A comparative study between grafted and nongrafted polymer films is provided. We demonstrate that the polymer grafting improves the protein adsorption by a factor of 75% and increase the cell spreading onto the polymer surface. Since the spreading is directly related to cell adhesion and proliferation, we demonstrate that the pNaSS grafting promotes PCL biocompatibility.OCIS codes: (160.1435) Biomaterials, (180.4315) Nonlinear microscopy