不同浓度MgSO_4和KH_2PO_4对香菇液体培养生长量的影响

本文研究了MgSO4 、KH2 PO4 二种无机物与香菇Cr- 0 2液体发酵生长量的关系。结果证明 :在 2 7℃摇床转速为 134r/min的培养条件下 ,MgSO4 浓度 0 0 5 % ,KH2 PO4 浓度为 0 10 % ,发酵 96h香菇生长量最大 ,发酵液透光率T在 6 30nm波长时达 135 8% ,其湿菌体产量32 6g/ 10 0mL ,发酵液由深褐色变成黄色、粘度大、透明 ,菌丝体个体较大且数目较多     

KH2PO4和赤霉素复合液对苦瓜种子发芽和根系生长的影响

研究不同浓度的KH2PO4和赤霉素复合液对苦瓜种子发芽的影响.结果表明:0.05% KH2PO4+100 mg/L赤霉素复合液对揭农槟城苦瓜的发芽率、发芽势和根系生长的促进效果最佳,对揭农大顶苦瓜和英香34的发芽率、发芽势和根系生长促进效果最佳复合液为0.1%KH2PO4+100 mg/L赤霉素,不同品种间的发芽率和发芽势存在差异.     

香菇液体培养的基本生理研究

供试的三个菌株７４０２，ｘ１６和大光在相同的有机培养基中，其商体量不同，以７４０２生长最多；其漆酶和多酚氧化酶活性也有差异，以Ｘ１６产生的最高。在有机基础培养基中加花生仁纷或葵籽仁粉可以促进菌体生长量，而加草纸浆却提高了木聚糖酶活性。以加漩甲基纤维素的综合培养基为种液，接种到有草纸浆加花生仁粉的培养基中，可以诱导出较高的纤维素酶活性，但漆酶和多酚氧化酶浩性不高。反之，用玉术粉为种液时，仅出刃了高的漆酶和多酚氧化酶活性，而纤维素酶性却是低的。     

不同水培条件对风信子生长的影响

以含有不同KH₂PO₄浓度控制不同的风信子水培条件,选择卡耐基品系大小均一的风信子鳞茎设置对照组进行试验,观察并记录不同水培条件下风信子叶芽、根系、花的生长等情况。初步总结不同水培条件对风信子生长的影响,结果表明:卡耐基品系的风信子在水培时,其根系在浓度为0. 05 mmol/ L KH₂PO₄水培条件下的长度与其他KH₂PO₄浓度水培条件下的风信子根系长度有显著差异。而在不同的KH₂PO₄浓度水培条件下,风信子的花期时长、花茎高度和叶片长度并没有显著差异。此外,试验还讨论证实适当的钾和磷可以提前风信子的花期、提高花的品质和观赏价值。     

香菇液体培养的工艺研究

运用单因素对比试验和正交试验,对香菇液体培养基的碳、氮营养源以及最佳配方和培养条件进行了优化研究,筛选出最佳碳源为麦芽糖,最佳氮源为麸皮,确定最佳培养基配方为麸皮80%、麦芽糖3%、MgSO40.15%、KH2PO40.1%;最佳培养条件为pH值5.5,摇瓶装液量100 mL/250 mL,接种量15%,摇瓶转速160 r/min。该研究为香菇液体发酵生产提供借鉴和理论依据。     

金针菇、草菇、香菇和红灵芝液体培养探讨

我室对几种常用食用菌液体发酵进行了初步试验,效果较好。下面我们将试验情况简单介绍如下。材料和方法(一)供试菌种:金针菇麓一号、草菇157号,香菇20号,红灵芝505号。以上菌种均由湖南师大菌种场提供。(二)培养基:1.斜面培养基:加富 PDA 培养基;2.原种培养基:麦粒种培养基;3.摇瓶培养基;(1)玉米粉3％,豆芽浆2％,葡萄糖1％,酵母膏0.5％,KH_2PO_40.1％,MgSO_40.1％;(2)玉米粉3％,豆芽浆2％,葡萄糖1％,酵母膏0.5％、甘蔗5％,KH_2PO_40.1％、MgSO_4     

赤霉素对香菇液体培养过程中发酵液生理生化的影响

以香菇菌株808为试材,研究了赤霉素对培养第9天～第17天香菇发酵液中的淀粉酶、羧甲基纤维素酶和漆酶3种胞外酶、胞外蛋白质活性的变化的影响,同时测定了第15天和第17天多糖以及生物量的变化.结果表明:淀粉酶在不添加赤霉素时酶活性达最高,最高达29.26 U;羧甲基纤维素酶在0.2 mg/L赤霉素浓度下酶活性最高,最高达31.50 U;漆酶在3.0 mg/L赤霉素浓度下酶活性最高,最高达30 U;3种胞外酶的最高值均出现在第15天左右.蛋白质浓度在2.0 mg/L赤霉素浓度下最高,第11天达高峰0.048 mg/mL; 2.0 mg/L赤霉素浓度下胞外多糖含量最高达23.64 g/L,1.0、2.0 mg/L赤霉素浓度下菌丝干重相对较高.     

不同液肥对万寿菊生物量的影响

采用不同浓度的液态肥(根宝和根动力)对万寿菊进行浇灌,对其地上地下生物量的测定比较.结果表明:根宝处理好于根动力处理,根宝施用浓度稀释1 500～2 500倍,能使根冠比明显增大,提高栽培质量.     

碳酸钠和碳酸氢钠对番茄壮苗和灰霉病的防治作用

以番茄品种L402幼苗为试材,研究外源喷施碳酸钠和碳酸氢钠对番茄生长和灰霉病的防治作用。结果表明:25mmol·L~(-1)和50 mmol·L~(-1)的碳酸钠与碳酸氢钠对番茄幼苗具有显著的壮苗作用;30～60 mmol·L~(-1)的碳酸钠和碳酸氢钠对灰霉菌菌丝生长具有不同程度的抑制作用,且抑制作用通常随着浓度的升高而加强;30～40 mmol·L~(-1)的碳酸钠和50～60mmol·L~(-1)的碳酸氢钠对番茄灰霉病具有良好的预防作用;50 mmol·L~(-1)的碳酸钠和50～60 mmol·L~(-1)的碳酸氢钠对番茄灰霉病具有良好的抑制作用。生产上可应用适宜浓度的碳酸钠和碳酸氢钠防治番茄灰霉病。     

外源NO和H2O2诱导不同颜色马铃薯花青素积累

以不同块茎颜色马铃薯品种陇薯8号、陇薯7号、LC310-2和山东彩肉为材料,研究外源NO、H2O2、NO+H2O2处理对淀粉积累期马铃薯块茎花青素合成及相关酶活性的影响。结果表明：经外源NO和H2O2处理后,4个基因型马铃薯薯皮和薯肉花青素含量均升高,PAL、PPO和CHI活性增强|诱导效果为0.1 mmol·L-1 SNP（NO的供体）+0.25 mmol·L-1 H2O2＞0.25 mmol·L-1 H2O2＞0.1 mmol·L-1 SNP＞清水（CK）,说明NO和H2O2处理对马铃薯花青素合成具有一定的促进作用,且表现为加性效应。     

Changes of PGE2, PGI2 and TXA2 concentration in rat traumatic pulpitis

AIM: To study the relationship between prostaglandins and acute pulpitis. METHODS: Rat traumatic pulpitis model was established by pulp exposure. The kinetic pathological changes in dental pulpal tissues and changes of PGE₂,6-Keto-PGF_1α and TXB₂ concentration in dental pulp were observed. RESULTS: After pulpal trauma, the dental pulp showed inflammatory changes and the concentrations of PGE₂,6-Keto-PGF_1α and TXB₂ were increased, which peaked at 6 hour post-trauma. CONCLUSION: Prostaglandins play a significant role in the pathogenesis of pulpitis.     

Lipoxin A4 reduces lipopolysaccharide-induced expression of cyclooxygenase 2 and prostaglandin E2 in human bronchial epithelial cells

AIM: To explore the effects of lipoxin A₄ on the expression of cyclooxygenase 2 (COX-2) in human bronchial epithelial cells (HBECs). METHODS: HBECs were incubated with various concentrations (0.1, 1 and 10 mg/L) of lipopolysaccharide(LPS) for 9 h, or 1 mg/L LPS for different time (3 h, 6 h and 9 h). The levels of COX-2 mRNA in HBECs and prostaglandin E₂ (PGE₂) in the culture supernatant were measured. In addition, the HBECs were exposed to lipoxin A₄ at concentration of 0, 100 and 400 μmol/L after stimulated with LPS at concentration of 1 mg/L for 9 h, and the supernatant of the culture cells was collected for determining the content of PGE₂ by ELISA. The cells were also harvested, and the mRNA and protein levels of COX-2 were analyzed by RT-PCR and Western blotting, respectively. RESULTS: LPS increased the mRNA expression of COX-2 and production of PGE₂ in a dose and time dependent manners in HBECs. Induction of COX-2 mRNA and protein by LPS were inhibited by lipoxin A₄ in a dose-dependent manner. Lipoxin A₄ also significantly decreased LPS-induced production of PGE₂. CONCLUSION: Lipoxin A₄ down-regulates LPS-induced expression of COX-2 and consequently inhibits the production of PGE₂ in HBECs.     

Effect of rates of Ca(OH)2 and ZnSO4 on soluble zinc concentration of spray solution and absorption of 65Zn by sweet-orange leaves

In a laboratory experiment, addition of increasing rates of Ca(OH)₂ to ZnSO₄ spray solution caused a decrease in soluble Zn concentration and an increase in its pH. The soluble Zn concentration in 0.15% neutralized ZnSO₄ solution was 7 times higher than in the 0.60% neutralized ZnSO₄ solution. The spray solutions containing 0.15 and 0.45% of ⁶⁵ZnSO₄ in combination with 0.0, 0.0375, 0.0750, 0.1125 and 0.225% of Ca(OH)₂ were applied to sweet orange (Citrus sinensis Osbeck) leaves. The increasing rates of Ca(OH)₂ in the ZnSO₄ spray solution decreased ⁶⁵Zn absorption by the leaves and this effect decreased with increasing rates of ⁶⁵ZnSO₄ in the solution. This has resulted from the decrease in Zn concentration in the spray solution. Thus addition or presence of any ion in the solution that forms a precipitate with Zn would cause a decrease in the efficiency of the spray solution as a result of a lower amount of Zn available from solution per unit area of the leaf.     

NH4NO3及碳源对红掌愈伤组织诱导影响的研究

以红掌的根段和叶片为外植体,探讨了不同NH3 NO4浓度和不同碳源组合对外植体愈伤组织诱导率的影响.结果表明:红掌再生对NH4 NO3的浓度非常敏感,低浓度的NH4 NO3有利于诱导愈伤组织,浓度较高时抑制愈伤的诱导;而不同的碳源也会影响到外植体愈伤组织的诱导,蔗糖与葡萄糖相比更适合于红掌愈伤组织的诱导.其中NH4 NO3 0 mg/L、蔗糖3％组合中根和叶片的愈伤组织诱导率最高,分别是92.6％和81.5％.     

等渗Ca(NO3)2和NaCl对番茄幼苗生长的影响

 等渗Ca(NO₃)₂和NaCl溶液对番茄幼苗具有不同的盐效应。28、56 mmol/L Ca (NO₃)₂溶液对其生长的抑制作用小于等渗的NaCl 溶液, 而84 mmol/L Ca (NO₃)₂溶液对幼苗生长的抑制程度与等渗的NaCl 溶液无显著差异。Ca(NO₃)₂主要通过渗透胁迫影响植株生长;而NaCl主要通过离子胁迫抑制植株的生长, 包括细胞质膜结构的破坏和K吸收的减少。