黄海冷水团海域微型异养鞭毛虫对异养细菌和蓝细菌摄食作用的初步研究黄海冷水团海域微型异养鞭毛虫对异养细菌和蓝细菌摄食作用的初步研究

2006年10月在黄海冷水团海域的三个站点开展了微型异养鞭毛虫、异养细菌和蓝细菌的密度和生物量调查,进行了微型异养鞭毛虫的现场摄食实验,通过荧光标记细菌法和消化系数法获得该海区微型异养鞭毛虫对异养细菌和蓝细菌的摄食率,并估算了微型异养鞭毛虫对异养细菌和蓝细菌现存量及生产力的摄食压。结果显示,微型异养鞭毛虫、异养细菌和蓝细菌的密度分别为036×103~113×103,039×106~113×106和004×104~374×104cells/cm3,温跃层以上明显高于底层。微型异养鞭毛虫对异养细菌的摄食率为533~1489个/（HF·h）,对蓝细菌的摄食率为026×102~2310×10-2cells/（HF·h）,摄食率随深度而下降。微型异养鞭毛虫每天能消耗927%~3308%的异养细菌现存量和812%~1609%的蓝细菌现存量。同时,微型异养鞭毛虫对异养细菌和蓝细菌的日摄食量各占它们生产力的266%~1310%和812%~1609%。研究表明微型异养鞭毛虫的摄食可能不是秋季黄海冷水团海域浮游细菌及其生产力的主归宿。     

黄海冷水团海域微型异养鞭毛虫对异养细菌和蓝细菌摄食作用的初步研究

2006年10月在黄海冷水团海域的三个站点开展了微型异养鞭毛虫、异养细菌和蓝细菌的密度和生物量调查,进行了微型异养鞭毛虫的现场摄食实验,通过荧光标记细菌法和消化系数法获得该海区微型异养鞭毛虫对异养细菌和蓝细菌的摄食率,并估算了微型异养鞭毛虫对异养细菌和蓝细菌现存量及生产力的摄食压。结果显示,微型异养鞭毛虫、异养细菌和蓝细菌的密度分别为0.36×103~1.13×103,0.39×106~1.13×106和0.04×104~3.74×104cells/cm3,温跃层以上明显高于底层。微型异养鞭毛虫对异养细菌的摄食率为5.33~14.89个/(HF·h),对蓝细菌的摄食率为0.26×102~23.10×10-2cells/(HF·h),摄食率随深度而下降。微型异养鞭毛虫每天能消耗9.27%~33.08%的异养细菌现存量和8.12%~16.09%的蓝细菌现存量。同时,微型异养鞭毛虫对异养细菌和蓝细菌的日摄食量各占它们生产力的2.66%~13.10%和8.12%~16.09%。研究表明微型异养鞭毛虫的摄食可能不是秋季黄海冷水团海域浮游细菌及其生产力的主归宿。     

黄海冷水团邻近海域浮游植物的昼夜垂直变化

于2006-10-19~20日在黄海南部海域进行定点(123°30′E,33°00′N)采样观测,用Uterm hl方法分析了浮游植物群集的垂直分布时间序列变化。研究结果表明:铁氏束毛藻(Trichodesmium thiebautiiGomont)为最优势种,其他优势种依次为具槽帕拉藻(Paralia sulcata(Ehrenberg)Cleve)、圆海链藻(Thalassiosira rotula Meunier)、菱形海线藻(Thalassionema nitzschioides Grunow)和佛氏海线藻(Thalassionema frauenfeldii Grunow)。硅藻在物种丰富度上占优势。调查期浮游植物细胞丰度介于(1.111~2 042.889)cells mL-1,平均值为192.756 cells mL-1。浮游植物细胞丰度垂直分布特征是表层水体最高,10m层迅速降低,底层最低。浮游植物细胞丰度随时间具有一定的波动,10月19日12:00和10月20日0:00在表、底层水体各出现2个细胞丰度峰值,10 m层的2个峰值比表、底层均滞后3 h。调查期浮游植物群集各层差异明显。     

黄海冷水团可培养细菌的多样性研究

本文研究了2008年7月黄海冷水团海域可培养细菌的多样性。AODC和DVC计数总菌数和活菌数分别为(1.3~4.8)×105/mL和(0.6~1.6)×105/mL;2216E平板以菌落计数法可培养细菌浓度为(0.56~2.2)×103cfu/mL。从分离到的475株中选取163株进行16S rDNA扩增,并用HhaⅠ酶进行ARDRA(扩增性rDNA限制酶切片段分析)多态性分析,取114株不同带型测序。结果显示,冷水团细菌归为4个细菌类群:变形菌门(Proteobacteria)、放线菌门(Acti-nobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes),共24个属,其中包括α-,β-,γ-变形菌纲;非冷水团细菌也归为这4个门类,共15个属,但未分离到β-变形菌纲。γ-变形菌纲在冷水团和非冷水团区域的不同深度都是优势菌群,不同的是冷水团区域α-变形菌纲比例较高(26.8%),而非冷水团区域α-变形菌纲比例相对较低(15.6%)。这表明黄海冷水团和非冷水团区域细菌多样性都很丰富,但其群落组成和优势菌群有所不同。此外,16S rDNA测序结果表明,6株细菌可能为海洋细菌新种。     

黄海冷水团的化学水文学特征

Based on the field data obtained during summer cruises in 2006, the overall perspective of chemical and hydrographic characteristics of the Yellow Sea Cold Water Mass(YSCWM) are discussed through the crossYSCWM transect profiles and horizontal distributions of hydrological and chemical variables, with emphasis on the differences between the northern Yellow Sea Cold Water Mass(NYSCWM) and the southern Yellow Sea Cold Water Mass(SYSCWM). The results show that YSCWM is characterized by low temperature(10°C) and dissolved oxygen(DO) concentration, high salinity(32.0) and nutrient concentrations. Compared to the SYSCWM, the NYSCWM possesses lower values of temperature, salinity and nutrient concentrations but higher values of DO.Also its smaller variation ranges of variables(except for temperature) demonstrate that NYSCWM is more uniform than that of SYSCWM. In addition, thermocline is more intensive in the SYSCWM than that of NYSCWM.Furthermore, DO and Chl a maxima appear at the depth of 30 m in the SYSCWM, while these phenomena are not obvious in the NYSCWM.     

黄海冷水团附近沉积物中的趋磁细菌及磁小体的特性研究

对黄海冷水团附近海域的沉积物进行了富集、荧光显微镜观察、电镜观察,并对其中大小均匀、形状规则的高密度颗粒进行了能谱分析。结果显示,在黄海冷水团附近有趋磁细菌和磁小体的存在。     

黄海冷水团演变过程及其与邻近水团关系的分析

黄海冷水团是出现在黄海的一种独特的水文现象.文中利用覆盖整个黄海的GDEM三维水温资料,结合近期一些大型调查所获得的有关观测研究结果,首先较系统地分析了黄海冷水团的形成和演变过程,并对冷水团3个冷中心的季节演变提出了一些与前不同的认识.同时,通过对黄海冷水团形成、发展和消亡与该海域温跃层演变关系的分析,进一步揭示了黄海冷水团演变的机理.然后,探讨了黄海冷水团演变过程中与青岛和仁川东南海域冷水团以及东海北部底层冷水的关系.分析表明,在黄海冷水团发展的鼎盛时期,青岛冷水团和仁川东南海域冷水团以及东海北部底层冷水皆包络其中.     

海洋岛海域在受黄海冷水团影响时期的水温特征

黄海冷水团作为夏季存在并有规律地逐月影响黄海海域的特殊水团,对于它的整体水文特征、形成原因、运动方式以及影响其强度的环境因素,曾有过许多研究和报道^[1-4].但是,关于冷水团势力影响海洋岛海域,在海洋岛海域形成的特殊水温特征却未见报道.本文将根据我们几年的水温观测结果结合有关冷水团的资料对黄海冷水团影响海洋岛海域所形成特殊水温特征及其形成原因做以下报道和分析.     

黄海冷水团海域浮游植物磷胁迫的季节变动

The Yellow Sea is located between the China Mainland and the Korean Peninsula, representing a typical shallow epicontinental sea. The Yellow Sea Cold Water Mass（YSCWM） is one of the most important physical features in the Yellow Sea. The characteristics of vertical profiles and seasonal variations of biogenic elements in the YSCWM may lead the variations of nutrient availability（e.g., phosphorus） and phosphorus stress of phytoplankton. In this study, the authors surveyed the seasonal variations of phytoplankton phosphorus stress with emphasis on the effect of the YSCWM during the four cruises in April and October 2006, March and August 2007. Using both bulk and single-cell alkaline phosphatase activity（APA） assays, this study evaluated phosphorus status of phytoplankton community, succession of phytoplankton community and ecophysiological responses of phytoplankton to phosphorus in the typical region of the YSCWM. With the occurrence of the YSCWM, especially the variations of concentration of dissolved inorganic phosphorus（DIP）, the results of bulk APA appeared corresponding seasonal variations. Along Transects A and B, the mean APA in August was the highest, and that in March was the lowest. According to the ELF-labeled assay＇s results, seasonal variations of the ELF-labeled percentages within dominant species indicated that diatoms were dominant in March, April and October, while dinoflagellates were dominant in August. During the four cruises, the ELF-labeled percentages of diatoms except Paralia sulcata showed that diatoms were not phosphorus deficient in April 2006 at all, but suffered from severe phosphorus stress in August 2007. In comparison, the ELF-labeled percentages of dinoflagellates were all above 50% during the four time series, which meant dinoflagellates such as Alexandrium and Scrippsiella, sustained perennial phosphorus stress.     

南黄海冷水团海域溶解氧和叶绿素最大现象值及营养盐累积的季节演变

基于2006-2007年在南黄海冷水团海域开展的4个季度月的调查资料, 重点研究了该海域溶解氧(DO)、叶绿素a(Chl a)最大值现象和营养盐累积的季节演变规律。结果表明:春至秋季黄海冷水团海域DO和Chl a最大值层深度具有先加深后变浅的趋势, 出现最大值层的海域面积呈现出先增大后缩小的变化过程, DO和Chl a最大值层的深度及面积在夏季均达到最大, 至冬季DO和Chl a最大值现象消失;夏季冷水团海域深水区DO最大值处的氧含量整体高于春季, 而冷水团边界附近氧最大值处的氧含量整体低于春季;春至秋季冷水域深水区次表层Chl a最大值处的Chl a含量先降低后升高, 于夏季时最低, 入秋后开始升高, 而一年四季中冷水域边界附近Chl a最大值处的Chl a含量却在夏季时最高, 而且显著高于深水区。黄海冷水团海域的底层营养盐储库具有一定的空间异质性, 冷水域底层通常分别在深水区和西部边界处存在营养盐高值核心, 其中位于深水区的高值核心位置季节变化不明显, 而位于冷水域西部边界附近的高值核心位置则呈现出自春季至夏季向西移动、入秋后又向东部移动的季节变化特征。水文物理因素和生物化学过程对DO、Chl a最大值及营养盐储库的季节演变具有重要的调控作用。     

The temporal and spatial variability of the Yellow Sea Cold Water Mass in the southeastern Yellow Sea, 2009-2011

The Yellow Sea Cold Water Mass(YSCWM) is one of the important water mass in the Yellow Sea(YS).It is distributed in the lower layer in the Yellow Sea central trough with the temperature less than 10 C and the salinity lower than 33.0.To understand the variability of the YSCWM,the hydrographic data obtained in April and August during 2009–2011 are analyzed in the southeastern Yellow Sea.In August 2011,relatively warm and saline water compared with that in 2009 and 2010 was detected in the lower layer in the Yellow Sea central area.Although the typhoon passed before the cruise,the salinity in the Yellow Sea central trough is much higher than the previous season.It means that the saline event cannot be explained by the typhoon but only by the intrusion of saline water during the previous winter.In April 2011,actually,warm and saline water(T >10 C,S >34) was observed in the deepest water depth of the southeastern area of the Yellow Sea.The wind data show that the northerly wind in 2011 winter is stronger than in 2009 and 2010 winter season.The strong northerly wind can trigger the intrusion of warm and saline Yellow Sea Warm Current.Therefore,it is proposed that the strong northerly wind in winter season leads to the intrusion of the Yellow Sea Warm Current into the Yellow Sea central trough and influenced a variability of the YSCWM in summer.     

北黄海冷水团温度年际变化研究

本文基于1976—2006年国家标准断面(大连—成山头)调查资料,结合ECMWF气温、风速以及辐射等再分析资料,研究了北黄海冷水团的低温中心以及北部锋面的年际变化规律,并对其与气候年际变化信号的关系做了相关性分析以及EOF分析,研究了影响北黄海冷水团的诸多因素。研究结果表明,北黄海冷水团及其北部锋面强度存在明显的年际变化特征,北黄海冷水团中心最低温度具有升温趋势,北部锋面强度具有减弱趋势。分析发现,前冬海温,当地气温,经向风场以及辐射通量都对来年北黄海冷水团的强度存在影响,东亚冬季风的年际变异是影响北黄海冷水团温度年际变化的主要机制,El Nio、La Nia事件成熟期滞后于北黄海冷水团最低温度的相对低值、高值出现,ENSO通过与东亚冬季风的相互作用与北黄海冷水团相联系。     

黄海冷水团水域浮游植物群落粒级结构的季节变化

根据2006—2007年度4个季节航次的实测资料,分析了黄海冷水团水域浮游植物叶绿素及其粒级结构的时空分布特征及季节变化规律,结果表明,在研究海域30 m以浅叶绿素总量的平均含量从高到低的顺序为:春季的(1.01 mg/m3)、夏季的(0.81 mg/m3)、秋季(0.72 mg/m3)、冬季(0.68 mg/m3);在叶绿素浓度大于1 mg/m3和小于1 mg/m3的区域浮游植物粒级结构差异较大,在整个研究海域,粒径较小的微型和微微型浮游植物对总生物量的贡献始终占主导(65%),粒径较大的小型浮游植物在冬季和春季贡献率相对较高;从季节尺度看,浮游植物的平均粒级指数从大到小的顺序为:春季的(15.47μm),冬季的(11.08μm),秋季的(8.61μm),夏季的(6.52μm);尽管不同季节水文和化学环境差异显著,但是不同粒径浮游植物的贡献率随总生物量的变化表现出一致性的规律。对环境因子与叶绿素分布的相关分析表明,浮游植物的生长在夏季主要受到营养盐来源的限制,冬季主要受到水体混合引起的光照限制,秋季可能受到磷酸盐和水体混合的共同限制。浮游植物粒级结构的分布格局主要是由各组分在不同环境中的资源竞争优势决定的。     

北黄海冷水团对獐子岛微微型浮游生物分布的影响

Picoplankton distribution around the Zhangzi Island(northern Yellow Sea)was investigated by monthly observation from July 2009 to June 2010.Three picoplankton populations were discriminated by flow cytometry,namely Synechococcus,picoeukaryotes and heterotrophic prokaryotes.In summer(from July to September),the edge of the northern Yellow Sea Cold Water Mass(NYSCWM)resulting from water column stratification was observed.In the NYSCWM,picoplankton(including Synechococcus,picoeukaryotes and heterotrophic prokaryotes)distributed synchronically with extremely high abundance in the thermocline(20 m)in July and August(especially in August),whereas in the bottom zone of the NYSCWM(below 30 m),picoplankton abundance was quite low.Synechococcus,picoeukaryotes and heterotrophic prokaryotes showed similar response to the NYSCWM,indicating they had similar regulating mechanism under the influence of NYSCWM.Whereas in the non-NYSCWM,Synechococcus,picoeukaryotes and heterotrophic prokaryotes exhibited different distribution patterns,suggesting they had different controlling mechanisms.Statistical analysis indicated that temperature,nutrients(NO3–and PO43–)and ciliate were important factors in regulating picoplankton distribution.The results in this study suggested that the physical event NYSCWM,had strong influence on picoplankton distribution around the Zhangzi Island in the northern Yellow Sea.     

黄海冷水团上升流对叶绿素垂向分布的影响

利用黄海冷水团物理-生态耦合模式，对冷水团水域叶绿素垂向分布的季节变化进行了数值模拟研究。物理模式为冷水团热动力模型，生态模式主要考虑叶绿素，营养盐和食植浮游动物基本状态变量的耦合方程。研究结果表明，黄海冷水团上升流对叶绿素垂向分布的夏季上层结构具有显著影响。整个夏季，受上升流的影响，叶绿素垂向分布最大值的位置向海面抬升，量值增大，混合层叶绿素的平均浓度增加，与实测资料比较表明，考虑冷水团上升流的影响比不考虑上升流与实测结果符合要好。     

黄海冷水团大型鲑科鱼类养殖研究进展与展望

黄海中部洼地的深层存在一个巨大的夏季冷水团,面积约13万km^2,水质优良,可以利用现代海洋装备在此海域养殖大型鲑科鱼类。中国海洋大学黄海冷水团鲑鱼养殖团队研发了养殖工船,概念性地设计了海浪能驱动的5万m^3的全潜式钢构网箱"深蓝1号"和约17万m^3的养殖-能源-管理于一体的"深蓝2号"智能钢构网箱,成功地完成了黄海冷水团鲑科鱼类养殖技术路线的验证。为实现规模化安全生产,仍需对养殖容量与黄海冷水团变动、适养种类及其育种、养殖技术、病害防治、饲料研发、机械化生产与智能化管理等进行深入研究。