E Sequence Analysis of Persistently Infected Mutant Japanese Encephalitis Virus Strains

Persistent infection is liable to occur after most RNA viruses infect the host. This phenomenon may be related to the geno-variation of the structural proteins or non-structural proteins the viruses contain, which helps the viruses to escape the killing function of the host im- mune protective system[1]. Japanese encephalitis virus (JEV) is a single-stranded, positive-sense RNA virus, which belongs to flaviviruses family. The mortality of epidemic B encephalitis is as high as 30 %, and 50 …     

Concentration and Purification of Japanese Encephalitis Virus from Infected Mouse Brains

A suspension was prepared from the infant mouse brain in-fected by JEV.It was clarified by centrifugation.Protamine sulfatewas added to the supernatant with a final concentration of 5 mg/ml,and the contaminated protein was removed.The recovery of HAand infectivity was 66% and 55% respectively.The supernatant was concentrated by differential centrifugationand purified by rate zone centrifugation in sucrose density gradientwith recovery of 29% of HA and 37% infectivity.The purificationfactors(PF)were 138 for HA and 175 for infectivity.SHA without any infectivity were found on the top of gradientcolumn.The immunological characteristics of the componentsawait further studies.     

Target therapy of ovarian carcinoma by monoclonal antibodies bearing chemical drugs entrapped in liposomes.

Monoclonal antibody COC166-9 against ovarian carcinoma was conjugated with adriamycin or cisplatinum entraped in liposomes as immunochemical liposomes MLA and MLP respectively. MLA was shown to have the highest effect than adriamycin or other groups on SKOV3 (ovarian cancer cell line) growth inhibition. MLA was also used in target therapy on nude mice bearing human ovarian carcinoma by xenograft of SKOV3 cells. The observations of tumor volumes revealed that this target therapy other than the controls presents a significantly better result which gives a hopeful clue to ovarian carcinoma treatment.

     

Production and Characterization of Monoclonal Antibodies Directed Against the Japanese B Encephalitis Virus

Six different hybridoma cell lines that produce monoclonal antibodiesdirected against the Japanese B encephalitis virus have been established by fusingSp2/0-Ag14 mouse myeloma cells with spleen cells form C×S/1 mice immunizedwith JE-SA14 virus.According to their cross-reactivities with four flaviviruses(Japanese B encephalitis virus,Saint Louis encephalitis virus,tick-borneencephalitis virus and dengue fever virus)using indirect immunofluorescenceassay,they were divided into three groups,type-specific,complex-specific andflavivirus group reactivity.At least six antigenic epitopcs on the surface ofJapanese B encephalitis virion were proposed based on functional analyses(haemagglutination inhibition,neutralization and passive mouse protection)directed against the four flaviviruses.Furthermore,a significant antigenicdifference among the Japanese B encephalitis virus strains isolated in China wasobserved with haemagglutination inhibition test.     

Japanese Encephalitis in China in the Period of 1950–2018:From Discovery to Control

Japanese encephalitis(JE)was first discovered in Japan in 1871;in 1924,a major outbreak occurred,with 6,000 JE cases reported and a mortality rate of approximately 60%[1,2].Later studies showed that JE is caused by the Japanese encephalitis virus(JEV),which is spread by mosquitoes.     

Antagonistic action of garlic extract and vitamin E to mutagenesis of cooking oil fumes

目的　观察大蒜提取物 ( garlicextract,GE)和维生素E在拮抗烹饪油烟凝集物 (condensatesofcook ingoilfume,COF)的致突变的作用。方法　在人外周血淋巴细胞培养物中注入一定剂量的GE或一定剂量的维生素E并一定量的COF ,观察淋巴细胞的姐妹染色单体交换率 (sisterchromatidexchanges ,SCE)的变化 ,挑选GE( 1× 10 - 4稀释 )和维生素E( 5 μl)同时注入培养基中 (含COF 10 μl) ,观察二者对COF的协同抗突变作用。结果　GE( 1× 10 - 4稀释 )可显著降低COF处理过的淋巴细胞SCE (P <0 .0 5 ) ;维生素E( 5 μl)和GE( 1× 10 - 4稀释 )在降低COF处理过的淋巴细胞SCE方面存在着协同作用 (P <0 .0 1)。结论　GE和维生素E在拮抗COF的致突变性过程中存在着显著的协同效应 ;一定量的GE亦有一定的拮抗COF的致突变作用     

Multichannel piezoelectric genesensor for the detection of human papilloma virus

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Suramin Inhibits the In Vitro Expression of Encephalitis B Virus Proteins NS3 and E

ThemortalityofepidemicencephalitisBvirusinfectionisveryhigh ,accountingforonethirdofalltheinfectedpatients[1] .Therefore ,itisofurgencytofindaneffectivewaytopreventandtreatthedisease .AlthoughtheinactivatedvaccineagainstencephalitisBvirusiswidelyused ,sofarnoefficaciousdrughasbeenfoundforthedisease .Suramin ,asananti trypanosomedrug ,camein tousein 192 0 ,the pharmacologicalmechanismofwhichremainsunclear .Apartfrombeingrichinan ions ,Suraminisuniqueintermsofitschemicalstruc ture .Itisassumed…     

Development of animal model for Chandipura virus in laboratory mice

本研究利用11个微卫星位点,对海南和广西恒河猴进行了遗传检测,并通过POPGEN32软件计算各个微卫星座位的等位基因频率、有效等位基因数目(Ne)、多态信息含量(PIC)和遗传杂合度(H)。结果表明,所选择的11个微卫星位点均存在高度的遗传多态性,H为0.6848~0.河河猴的Ne、PIC和H的平均值均高于海南猴,分别为4.2583、0.7090、0.7706和4.2054、0.7025、0.7656,这种差别可能与地域来源有关。这些研究为微卫星标记分析恒河猴遗传多样性提供理论基础。     

脂质体对JEV－E糖蛋白的佐剂作用研究

目的：观察脂质体对日本脑炎病毒Ｅ糖蛋白的佐剂作用；方法：利用改进的振荡法，使亲和层析提取的日本脑炎病毒Ｅ糖蛋白包裹于脂质体中，制备出脂质体－Ｅ蛋白结合物，并分组免疫ＢＡＬＢ／ｃ小鼠；通过ＥＬＩＳＡ，血凝抑制试验（ＨＩ）和中和试验（ＮＴ）等指标观察．结果：脂质－Ｅ蛋白免疫组的特异性抗体水平明显高于游离Ｅ蛋白组；在两批病毒攻击保护性试验中，前者保护率分别为５０％和５７％，后者分别为３８％和３０％．结论：脂质体对日本脑炎病毒Ｅ糖蛋白有明显的佐剂作用．     

云南省马关县蚊虫调查及与乙型脑炎病毒的关系

1990年 8月在马关县捕获成蚊 4属 16种 2 0 95只 ,其中库蚊 8种 ,按蚊 6种 ,伊蚊 1种 ,阿蚊 1种。三带喙库蚊、伪杂鳞库蚊、中华按蚊和骚扰阿蚊为优势蚊种。该县人群血清中乙型脑炎病毒抗体阳性率 12 .0 7%(7/5 8)。三带喙库蚊和伪杂鳞库蚊可能是当地乙型脑炎病毒的传播媒介。     

人类β干扰素对乙脑病毒在人类细胞系上增殖的抑制作用

本文研究了人类β干扰素对乙型脑炎病毒在３株人类细胞株上的增殖的抑制作用（Ｈｅｐ－２，ＦＬ和Ｕ９３７）。人类β干扰素在１０００Ｉμ／ｍｌ时，病变产生在ＲＬ细胞，Ｈｅｐ－２细胞和Ｕ９３７细胞上分别是对照组的０．５％，２．７％，３７．２％。本研究同时比较了人类β干扰素在人类细胞株，猴细胞株和地鼠细胞株上对乙脑病毒的抑制作用（Ｈｅｐ－２，Ｖｅｒｏ，ＢＨＫ２１）其敏感性有明确的差异（许多文献已有报道）。本研     

云南省金平县蚊虫及其与流行性乙型脑炎病毒关系的调查

1990年8月在金平县捕获成蚊4属18种5654只,其中库蚊9种、按蚊7种、伊蚊1种、阿蚊1种。中华按蚊、伪杂鳞库蚊和三带喙库蚊为优势蚊种。该县人群血清中乙型脑炎病毒抗体阳性率为21.33％(16/75)。三带喙库蚊和伪杂鳞库蚊可能是当地乙型脑炎病毒的传播媒介。     

广东和广西部分地区蝙蝠携带登革病毒及流行性乙型脑炎病毒的调查研究

目的了解广东及广西蝙蝠携带登革病毒和流行性乙型脑炎(乙脑)病毒的情况。方法2004年9月至2005年11月期间11次收集广东及广西部分地区的蝙蝠。收集的蝙蝠取脑组织标本和血清标本,采用两对登革病毒和乙脑病毒通用引物,用逆转录-聚合酶链反应(RT-PCR)和细胞培养分离方法检测所采集蝙蝠标本中的登革病毒(denguevirus,DV)和乙脑病毒(Japaneseencephalitisvirus,JEV)。结果收集了4个科9个种共905只蝙蝠,其中棕果蝠154只、犬蝠395只、普通长翼蝠5只、小黄蝠52只、中菊头蝠228只、中华菊头蝠15只、小菊头蝠41只、双色蹄蝠8只、普氏蹄蝠7只。蝙蝠来源于粤中、粤西和桂东的7个县市。905只蝙蝠标本中未检测、分离到登革病毒和乙型脑炎病毒。结论未发现所调查的蝙蝠携带登革病毒和乙型脑炎病毒。     

乙型脑炎病毒与黄热病病毒联合诊断基因芯片的初步实验研究

目的制备乙型脑炎病毒（JEV）与黄热病病毒（YFV）联合诊断基因芯片。方法基于早期实验室研究挑选出28条寡核苷酸探针制备检测芯片。克隆于质粒上的乙型脑炎病毒和黄热病病毒基因片段用于检测探针特异性，经限制性显示技术扩增并标记，与芯片杂交后完成扫描和数据分析。结果JEV、YFV探针与相应的荧光标记样本杂交后，均能检测出阳性荧光信号，而空白对照则检出阴性信号。结论基因芯片技术为病毒检测提供了一种早期、可靠的方法，具有应用于多病毒临床鉴别诊断的前景。     

流行性乙型脑炎的流行病学

流行性乙型脑炎病毒是在世界范围内引起病毒性脑炎的重要原因之一,每年乙脑的发病约50000例,其中15000例死亡。乙脑的流行和散发病例多发生在亚太地区。本文将对乙脑的流行病学作一综述。     

云南省孟连县蚊虫调查及乙型脑炎病毒分离

1983年8月,在云南省孟连县捕获成年雌性蚊虫5属19种3773只。三带喙库蚊、霜背库蚊、棕头库蚊和中华按蚊是农村畜圈的主要蚊种,白纹伊蚊是野外竹林的优势蚊种。从三带喙库蚊和雪背库蚊中各分离出1株乙型脑炎脑毒。分析认为,三带喙库蚊是当地乙型脑炎病毒的主要传播媒介,雪背库蚊亦参予乙型脑炎病毒的传播。