严重肢体缺血患者血浆中促血管生成潜力与血浆MMP-9、TIMP-1和TIMP-2水平升高的相关性研究（英文）

本研究通过比较间歇性跛行(IC)和严重肢体缺血(CLI)患者的血液参数,选择合适的临床和生化特指标,以评估促血管生成的潜力和抑制血管生成的作用.结果表明,通过刺激下肢动脉疾病(LEAD)病人血浆中的血管生成,内皮生长因子A(VEGF-A)浓度会显著增加,同时依赖于循环受体sVEGFR-1和sVEGFR-2的抑制也会显著减少.与IC病人相比,CLI病人具有较高的VEGF-A、金属蛋白酶9(MMP-9)、金属蛋白酶组织抑制因子1(TIMP-1)和TIMP-2浓度.     

Overweight and obesity versus concentrations of VEGF-A,sVEGFR-1, and sVEGFR-2 in plasma of patients with lower limb chronic ischemia

Objective

Being overweight or obese comprises a significant risk factor for atherosclerosis. Fat tissue also generates factors stimulating angiogenesis, the process by which new blood vessels form. The purpose of this paper is to assess concentrations of the vascular endothelial growth factor A (VEGF-A) and its soluble type-1 and type-2 receptors (sVEGFR-1 and sVEGFR-2) in plasma of patients with peripheral arterial disease (PAD) depending on the level of nutrition according to body mass index (BMI).

Methods

The study group included patients suffering from symptomatic PAD (n=46) in Fontaine classes IIa–IV without any history of neoplastic disease and who have a normal BMI (n=15), are overweight (n=21) or are obese (n=10). The control group (n=30) consisted of healthy non-smoking volunteers who were neither overweight nor obese. Venous blood plasma samples were collected from both groups at rest in the morning to determine plasma concentrations of VEGF-A, sVEGFR-1, and sVEGFR-2 using the enzymelinked immunosorbent assay (ELISA) method.

Results

The group of patients with PAD co-existent with being overweight or obese tended to have higher mean concentration levels of VEGF-A and sVEGFR-2 when compared with patients suffering from PAD with normal BMI. A statistically significant positive correlation was obtained between BMI and average plasma concentrations of sVEGFR-2 (R=0.37, P=0.0103). However, no significant correlation was noticed between BMI and VEGF-A or sVEGFR-1 concentrations.

Conclusions

A positive correlation determined between the level of antiangiogenic factor and BMI value may be indicative of the linearly growing prevalence of some antiangiogenic factors in patients with metabolic disorders, which may be one of numerous factors contributing to incomplete efficiency of collateral circulation development in patients with PAD.

     

育龄期功能失调性子宫出血女性子宫内膜组织中MMP-9、TIMP-1的表达变化与意义

目的:研究育龄期功能失调性子宫出血患者子宫内膜局部MMP-9、TIMP-1的表达变化。方法:采用免疫组化S-P法检测35例育龄期功能失调性子宫出血患者及20例正常月经健康女性排卵后期(即对照组)子宫内膜MMP-9、TIMP-1的表达情况。结果:育龄期功血女性以排卵型为主(30/35),其子宫内膜组织中MMP-9在出血部位的表达显著高于正常部位及对照组(P<0.05),TIMP-1在出血部位的表达显著低于正常部位及对照组(P<0.05),有统计学意义。结论:子宫内膜局部MMP-9与TIMP-1失衡与育龄期功血的发生有关。     

让新课改真正走入物理教学

推进素质教育,面向全体学生,是教育改革和发展的根本任务。作为义务教育的重要课程——物理,如何进行改革,是摆在每一位物理教师面前的严峻任务,既要减轻学生负担,又要把最基础的内容传授给学生;既要为高一级学校的选拔培养人才,又要兼顾全体。物理教师的任务,任重而道远。     

复发性自然流产患者血清RLX-2,TIMP-1,TIMP-2的表达及其临床诊断价值

目的检测复发性自然流产（RSA）患者血清RLX-2,TIMP1,TIMP2的表达水平,探讨RLX-2,TIMPs在RSA发生过程中的作用机制,及其对RSA的临床诊断价值。方法采用ELISA法对18例RSA患者与18例正常妊娠者不同妊娠期血清RLX-2,TIMP1,TIMP2的表达水平进行检测,采用受试者工作特性曲线对血清RLX-2,TIMP2及RLX-2/TIMP-2比值在RSA临床诊断的价值进行评价。结果血清RLX-2的水平在妊娠呈上升趋势,10～12周达到高峰;实验组血清TIMP-2水平在各妊娠期均高于正常组（P〈0.05）,各妊娠期RLX-2/TIMP-2比值的曲线下面积（AUC）均较RLX-2,TIMP-2高,其最佳拐点为≤0.1（P〈0.000 1）。结论RSA的发生可能与妊娠期RLX-2的分泌不足以及过高的血清TIMP-2浓度有关;妊娠10～12周时RLX-2/TIMP-2比值≤0.1对RSA的临床诊断价值最大。     

乳腺癌组织中MMP-2、MMP-9表达及预后意义

目的：探讨基质金属蛋白酶MMP-2、MMP-9在乳腺癌中的表达情况及与临床预后的关系。方法：采用组织芯片及免疫组化S-P法检测了183例乳腺癌组织中MMP-2、MMP-9的表达水平。结果：①乳腺癌组织中MMP-2和MMP-9的阳性表达率分别为77．0％（141／183）和65．6％（120／183）。乳腺癌组与良性对照组MMP-2、MMP-9的表达具有显著性差异（P〈0．01）。②MMP-2的表达与肿瘤大小有关（P=0．010），与临床TNM分期、淋巴结转移状态、组织学分级密切相关（P均〈O．01）；MMP-9的表达与组织学分级有关（P=0．012），与肿瘤大小、临床TNM分期、淋巴结转移状态密切相关（P均〈O．01）；MMP-2和MMP-9的表达与年龄、月经状态、病理类型以及雌孕激素受体状态无关。③MMP-2、MMP-9高表达组10年生存率均低于低表达组。结论：MMP-2、MMP-9均可作为乳腺癌患者预后判断的指标。     

Effect of enalapril on plasma homocysteine levels in patients with essential hypertension

Objective:To investigate the effect of enalapril on plasma homocysteine(Hcy) levels and the association of methylenetetrahydrofolate reductase(MTHFR) C677T polymorphism with the changes of Hcy levels in response to enalapril among patients with essential hypertension.Methods:A total of 130 patients with mild-to-moderate essential hypertension were enrolled and enalapril was orally administered at a dose of 10 mg/d for eight weeks.Plasma Hcy levels were measured by denaturing high-performance liquid chromatography(DHPLC) at baseline and after eight weeks of treatment.Genotyping of MTHFR C677T polymorphism was performed by TaqMan probe technique.Results:Compared with baseline,plasma Hcy levels did not change significantly after eight weeks(P=0.81).Stratified by baseline Hcy levels,a significant increase in plasma Hcy levels(P=0.02) among those with Hcy 10 μmol/L was observed,in contrast to no significant changes in plasma Hcy levels(P=0.54) among those with Hcy ≥10 μmol/L.No significant association was observed between MTHFR C677T polymorphism and changes in Hcy levels in response to enalapril.Conclusions:Enalapril may cause an increase in plasma Hcy levels among the hypertensives with low baseline Hcy levels.There was no significant association between MTHFR C677T genotypes and changes in Hcy levels in response to enalapril among subjects with essential hypertension.     

Clinical characteristics of critical patients with pandemic influenza A (H1N1) virus infection in Chengdu, China

Objective  

The critical illness of pandemic influenza A (H1N1) virus infection may be associated with relatively poor outcomes. The objective of this study is to describe clinical features and factors associated with the deaths of critical patients.     

严重烫伤早期大鼠脑内MMP-9的表达及意义

目的:探讨MMP-9与严重烫伤后血脑屏障损伤的关系.方法:健康SD大鼠随机分为正常组、假烫伤组、烫伤组,烫伤后又设3、6、12、24、48 h 5个时相点,用透射电镜观察血脑屏障超微结构变化;干温重法测脑含水量;紫外光度计检测脑EB含量;免疫组化观察脑MMP-9表达.结果:烫伤后大脑皮质的神经细胞线粒体肿胀或溶解,突触小泡减少,突触间隙增宽.脑毛细血管周围出现空泡化,内皮肿胀,基底膜厚薄不均.严重烫伤后脑含水量和EB含量明显增高,MMP-9表达升高,差别具有统计学意义(P<0.01).结论:严重烫伤后大鼠血脑屏障通透性增高与脑内MMP-9表达升高有关,提示MMP-9是严重烫伤后血脑屏障损伤的关键因素.     

A preliminary evaluation of VEGF-A,VEGFR1 and VEGFR2 in patients with well-controlled type 2 diabetes mellitus

Objective

Decompensated chronic hyperglycemia often leads to late microvascular complications such as retinopathy, diabetic foot syndrome, and diabetic kidney disease. The aim of this study was to determine the concentration of vascular endothelial growth factor A (VEGF-A) and its receptors in patients with well-controlled diabetes.

Methods

The study was conducted on 31 patients with well-controlled type 2 diabetes without micro-or macroangiopathy. Thirty healthy volunteers were enrolled in a control group. Serum concentrations of VEGF-A, VEGF receptors 1 and 2 (VEGFR1 and VEGFR2), fasting glucose, and lipid profiles were measured, and the plasma concentration of glycated hemoglobin (HbA1c) was determined.

Results

No significant differences were observed between the concentration of VEGF-A, VEGFR1 or VEGFR2 in the subject group and that in the control group. Positive correlations were noted between the levels of VEGF-A, VEGFR2, and triglyceride, and there was a negative correlation between the levels of VEGFR2 and high-density lipoprotein (HDL)-cholesterol in the study group.

Conclusions

The concentrations of VEGF-A and its receptors 1 and 2 in patients with well-controlled diabetes are comparable to those of healthy individuals, which may indicate that appropriate control of glucose levels delays the occurrence of vascular complications. A negative correlation between VEGFR2 and HDL-cholesterol levels, and positive correlations between VEGF-A, VEGFR2, and triglyceride levels, suggest that lipid abnormalities occurring in diabetes may be involved in the modulation of angiogenesis.     

实验性兔脑血管痉挛血清MMP-9及VEGF的变化研究

观察血清基质金属蛋白酶-9（MMP-9）及血管内皮生长因子（VEGF）在实验性新西兰白兔蛛网膜下腔出血后的浓度变化,探讨它们与脑血管痉挛发生、发展及转归过程的关系,为临床诊断及治疗提供理论依据。实验结果表明血清MMP-9及VEGF的浓度变化可以作为预测蛛网膜下腔出血后脑血管痉挛的发作、判断其发展及预后的参考指标之一,为临床诊断与治疗提供依据。MMP-9及VEGF参与了蛛网膜下腔出血后的病理生理过程,与迟发性脑血管痉挛的发病机制有密切关系。     

基质金属蛋白酶-2在乳腺癌中的表达及与侵袭、转移的关系

[目的]探讨乳腺癌细胞基质金属蛋白酶-2的表达及其与侵袭、转移的关系.[方法]免疫组织化学方法检测60例乳腺癌患者癌组织中MMP-2表达.[结果]MMP-2的表达在乳腺癌细胞明显高于正常乳腺细胞(P<0.01);淋巴结转移组明显高于非淋巴结转移组(P<0.01);在中、低分化组明显高于高分化组(P.<0.01).但与组织学类型无关(P<0.05).[结果]乳腺癌的MMP-2的表达与其侵袭、转移可能相关.     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

ＩＮＴＲＯＤＵＣＴＩＯＮＳｉｎｃｅｔｈｅｆｉｒｓｔｓｕｃｃｅｓｓｆｕｌｉｓｏｌａｔｉｏｎｏｆＨ .ｐｙ ｌｏｒｉｉｎ 1 982 ,Ｈ .ｐｙｌｏｒｉｉｎｆｅｃｔｉｏｎｈａｓｂｅｅｎｆｏｕｎｄｔｏｂｅａｓｓｏｃｉａｔｅｄｗｉｔｈｖａｒｉｏｕｓｄｉｓｅａｓｅｓｉｎｃｌｕｄｉｎｇｃｈｒｏｎｉｃｇａｓｔｒｉｔｉｓ,ｐｅｐｔｉｃｕｌｃｅｒｄｉｓｅａｓｅｓ(Ｄｏｏｌｅｙｅｔａｌ.,1 989;Ｇｒａｈａｍｅｔａｌ.,1 992 ) ,ａｎｄｇａｓｔｒｉｃｎｅｏｐｌａｓｍｓ(Ｂａｙｅｒｄｏｒｆｆｅｒｅｔａｌ.,1 995) .Ｉｔｗａｓｓｕｇｇｅｓｔ…     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) and Helicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status of H. pylori was determined by histologic examination, RUT, 14C - UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients with H. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml, P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection of H. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml,respectively (P<0.05). The serum levels of sICAM-1 proved to be significantly correlated with the density of H. pylori colonization in gastric mucosa (rs =0.316, P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated that H. pylori infection up-regulates the expression of sICAM-1.     

关于带第一特征值λ1具Sobolev临界指数2*的一类椭圆方程-Δu=λ1u+|u|2*-2u+τ(x,u)

给出了半线性椭圆方程-Δu=λ1u+|u|2*-2u+τ(x,u)的Dirichlet问题在对扰动项τ(x,u)增加适当条件后非平凡解的存在性定理,以及方程-Δu=λu-|u|2*-2u+h(x),λ∈[λ1,λk](这里λk是方程-Δu=λu的第k个互不相等的特征值)的非零解的存在性定理.     

未受控制的高血糖对2型糖尿病患者粘附分子的影响（英文）

目的:评估可溶性血管细胞间黏附分子(s VCAM-1)、可溶性细胞间黏附分子(s ICAM-1)、可溶性选择素E和可溶性血栓调节蛋白在血糖控制良好和不受控制的2型糖尿病患者中的水平。创新点:对2型糖尿病患者的血管内皮炎症标记物进行评估。方法:62例糖尿病患者分成两组:第一组包括35个血糖控制良好的糖尿病患者,第二组包括27个未控制血糖并伴有微蛋白尿的糖尿病患者。对照组由25名健康志愿者组成。测定血浆中s VCAM-1、s ICAM-1、可溶性选择素E和可溶性血栓调节蛋白的浓度,同时测定血清肌酐及血浆中空腹血糖和糖化血红蛋白(Hb A1c)的浓度。结论:与血糖控制良好的糖尿病组相比,未控制血糖组具有相对低的ICAM-1水平和更高的VCAM-1水平。未控制血糖组中患者的糖化血红蛋白和ICAM-1之间呈正相关,肾小球滤过率和可溶性选择素E之间呈正相关,而肌酐和ICAM-1之间呈负相关。研究证实2型糖尿病的发病机理中炎症过程的出现与血管内皮功能受损有关。未受控制的高血糖对粘附分子的反向作用表明,在糖尿病的并发症中VCAM-1和ICAM-1具有不同功能。     

Aircraft noise exposure affects rat behavior,plasma norepinephrine levels,and cell morphology of the temporal lobe

In order to investigate the physiological effects of airport noise exposure on organisms, in this study, we exposed Sprague-Dawley rats in soundproof chambers to previously recorded aircraft-related noise for 65 d. For comparison, we also used unexposed control rats. Noise was arranged according to aircraft flight schedules and was adjusted to its weighted equivalent continuous perceived noise levels (L _wecpn ) of 75 and 80 dB for the two experimental groups. We examined rat behaviors through an open field test and measured the concentrations of plasma norepinephrine (NE) by high performance liquid chromatography-fluorimetric detection (HPLC-FLD). We also examined the morphologies of neurons and synapses in the temporal lobe by transmission electron microscopy (TEM). Our results showed that rats exposed to airport noise of 80 dB had significantly lower line crossing number (P<0.05) and significantly longer center area duration (P<0.05) than control animals. After 29 d of airport noise exposure, the concentration of plasma NE of exposed rats was significantly higher than that of the control group (P<0.05). We also determined that the neuron and synapsis of the temporal lobe of rats showed signs of damage after aircraft noise of 80 dB exposure for 65 d. In conclusion, exposing rats to long-term aircraft noise affects their behaviors, plasma NE levels, and cell morphology of the temporal lobe.     

关于图ω4,6的（r1,r2,…,r9）-冠的优美性

给出了ω46,的(r1,r2,…,r9)-冠的定义,讨论了ω46,的(r1,r2,…,r9)-冠的优美性,用构造性的方法给出了一些特殊的ω46,的(r1,r2,…,r9)-冠的优美标号。     

论高职课程中科学、技术、人文的融合

高职教育的双重属性必须通过课程来体现.在设置高职课程目标时,应先厘清科学、技术、人文三者在课程中的关系,然后按照高职教育的双重属性,构建多元整合型课程,将科学、技术、人文三者衔接沟通,恰当地融合在高职课程体系中,培养集真善美于一身的高素质人才.高职课程建设任重道远,必须坚持三者辩证统一.     

New media,evolving multimodal literacy practices and the potential impact of increased use of the visual mode in the urban environment on young children's learning

This article looks at the way in which the changing visual environment affects education at two levels: in communication patterns and research methodologies. The research considers differences in the variance and quantity of types of visual media and their relationship to the written mode in the urban landscapes of Tokyo and London, using Google Street View to make comparisons. It reflects on the parallels this might have in the ways in which children encounter text in the environment. The data were analysed using Visual Content Analysis and colour coding. It found the Tokyo sample to have a higher density and diversity of visual media types at a wider range of heights. Visual and written communication also appear to be confined to more separately definable spaces with more equal weighting in the use of written and visual modes. Particularly, within the context of early childhood education it is hoped these findings will increase knowledge concerning young children's exposure to the visual mode, initiate wider discourse around this less researched mode's role within new multimodal communication practices and that the methodology furthers understanding of the potential of new media in image‐based research.