覆盆子活性物质提取工艺的优化

采用闪式提取法,以多糖和黄酮为观测指标,利用L9(34)正交试验对覆盆子活性物质的提取工艺进行了优化研究。优化的工艺条件为:料液比1:20;提取电压120 V,提取次数4次(30s/min)。料液比例、提取电压对提取影响显著,对比不同提取方式对覆盆子活性物质的提取率显示,闪式提取超声提取普通提取。闪式提取覆盆子多糖得率较普通提取提高124.80%,黄酮提高130.31%,较超声提取,闪式提取覆盆子多糖得率提高33.20%,黄酮提取提高30.17%。对该工艺方法进行验证,得到覆盆子多糖、黄酮的得率分别为27.56 mg/g和23.86 mg/g,表明闪式提取工艺省时、高效、节能,适宜于覆盆子活性成分的提取。     

覆盆子干果黄酮提取研究

覆盆子为我国一种药食两用果实,具有极高营养与保健功效.通过单因素及响应面分析试验,研究了乙醇浓度、浸提温度、浸提时间、料液比对覆盆子干果黄酮提取的影响,结果表明,四者都有不同程度的影响.经响应面方法优化,得出覆盆子黄酮提取的最佳工艺参数为:乙醇浓度91％,提取温度74℃,提取时间8.3 h.此条件下,方程预测值和验证试验值相差1％,覆盆子黄酮的最大提取量达到2.53 mg/g.     

覆盆子复合保健饮料的研究

以中草药覆盆子为基料,以何首乌、银杏、黄精为营养辅料,通过试验探讨了覆盆子浸提工艺条件以及覆盆子复合保健饮料的制配工艺条件.正交实验表明覆盆子浸提工艺条件为:浸提温度65℃,固液比1:30,加酶量0.7 g/100 mL,浸提时间60 min.保健饮料较佳的配伍条件为:覆盆子原汁用量40 mL,蔗糖用量3.00 g/100 mL,柠檬酸用量O.10 g/100 mL.     

石耳多糖的提取工艺研究

采用单因素试验和正交试验设计,探讨提取时间、料液比和提取温度对石耳多糖提取率的影响,筛选出了最佳提取工艺。结果表明,影响石耳多糖提取率的主次因素为料液比、提取温度及提取时间;石耳多糖最佳提取工艺条件为:提取温度80℃,提取时间4h,料液比1∶10。在此工艺条件下,水溶性石耳多糖的提取率为4.63%。     

响应面法优化机械力辅助提取覆盆子多糖

为了探究覆盆子多糖的最佳提取工艺,在单因素试验的基础上,采用响应面法考察了机械力辅助下的球磨时间、球磨转速、球料比对覆盆子多糖得率的影响.结果显示:在球磨时间为9.0min,球磨转速为363r/min,球料比为298:1,超声时间为20min,液料比为30:1的条件下,覆盆子多糖得率最高为11.05％.在超声提取、回流...     

黑玛咖多糖超声辅助提取研究

以黑玛咖为原料,多糖得率为指标,研究提取温度、超声时间、料液比对玛咖多糖得率的影响,采用苯酚–硫酸法测定玛咖多糖的含量。以单因素试验和正交试验优化提取条件,最佳提取工艺条件为提取温度50℃、超声时间30 min、料液比1∶40(g/mL),利用方差分析表明影响多糖提取的主次因素为提取温度超声时间料液比,在最佳条件下多糖得率为9.03%。     

黑松松针多糖提取工艺研究

以黑松松针为原料,在单因素实验基础上,选择提取温度、提取时间、料液比为影响因素,松针多糖提取率为指标,进行正交试验,研究各因素对松针多糖提取率的影响。结果显示:提取温度和提取时间是主要影响因素,料液比影响较小,最佳提取工艺为提取温度100℃、提取时间4 h、料液比1∶10 g/m L,此条件下黑松松针多糖得率为2. 640%。     

覆盆子啤酒的研究

采用响应面法Box-Behnken设计和正交实验设计对适宜于覆盆子啤酒生产的覆盆子黄酮复合提取工艺及添加工艺进行了优化研究,确定了适宜的提取工艺为:提取时间10 h,提取温度60℃,果胶酶添加量0.90 g/L,p H4.5,此条件下,覆盆子黄酮提取最大值为20.60 mg/g;适宜的添加工艺为:覆盆子提取液添加量3%,啤酒花添加量0.015%,覆盆子提取液添加时间为啤酒过滤前添加。     

超声波法提取虫草花多糖工艺的研究

通过超声波法对虫草花中多糖的提取条件进行优化。超声波法提取虫草花多糖的最佳提取工艺条件可由料液比、超声时间、超声温度来控制,最后确定虫草花多糖最大提取率的条件为料液比1∶40,超声时间30min,超声温度50℃,在此条件下测得的虫草花多糖提取率为4.85g/100g。     

油茶枯中皂素和多糖的综合提取工艺研究

研究了油茶枯中皂素和多糖的综合提取工艺,采用95%乙醇提取皂素,再用水提取多糖。考察了提取温度、提取时间、料液比、提取次数对皂素提取率以及多糖提取率的影响。分别进行正交实验,得到皂素的最佳提取工艺条件为:提取温度80℃、料液比1∶10、提取次数2次、提取时间1.5h,此时皂素提取率达到97.5%;多糖的最佳提取工艺条件为:提取温度70℃、提取时间2.5 h、料液比1∶12,此时多糖提取率达到84.6%。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press     

INFOPRINT 2014 was Successfully Held in Dongguan

Sponsored by Printing and Printing Equipment Industries Association of China （PEIAC） and organized by Print China magazine, the Seventeenth Beijing International Printing Information Conference （INFOPRINT 2014） was successfully held on 11th Dec. 2014 at Dongguan Exhibition International Hotel.