胃肠道平滑肌肿瘤核仁组成区嗜银蛋白的研究

本文应用胶银染色法检测胃肠道平滑肌肿瘤 (ｇａｓｔｒｏｉｎ ｔｅｓｔｉｎａｌｓｍｏｏｔｈｍｕｓｃｌｅｔｕｍｏｒｓ,ＧＩＳＭＴ)中核仁组成区嗜银蛋白 (ａｒｇｙｒｏｐｈｉｌｉｃｎｕｃｌｅｏｌａｒｏｒｇａｎｉｚｅｒｒｅｇｉｏｎｓｐｒｏｔｅｉｎｓ,ＡｇＮＯＲｓ)的表达情况 ,旨在探讨其临床病理及预后价值。材料与方法一、临床病理资料从本院 1977～ 1996年 4月间手术切除的ＧＩＳＭＴ标本中 ,选择诊断明确、病历完整者 86例。其中男 49例 ,女 37例。年龄为 1～ 77岁。肿瘤发生于食管 11例 ,胃 30例 ,小肠 2 7例 ,结直肠 18例。以核分…     

肝炎,肝硬化和肝癌的核仁组成区相关蛋白的研究

本文采用嗜银染色技术对慢性活动性肝炎、肝硬化和肝癌的核仁组成区相关蛋白(AgNOR)进行了计数和探讨。结果显示:慢性活动性肝炎的AgNORs核均数是2.69±1.04,肝硬化为2.72±0.69,均高于正常肝细胞的AgNORs,核均数1.80±0.56(P<0.05),但低于肝细胞性肝癌的AgNORs核均数5.67±2.51(P<0.01)。随着肝癌的分化程度变差,肝癌的AgNORs核均计数增加。我们认为AgNOR技术有助于某些肝脏良恶性病变的诊断和肝癌的病理学分级,有望成为肝肿瘤研究的一个新指标。     

慢性肝病,肝细胞癌及其癌旁肝组织核仁组成区嗜银蛋白的研究

采用嗜银染色技术研究正常肝、慢性肝病、肝细胞癌及其癌旁肝组织核仁组成区嗜银蛋白（Ａｇ－ＮＯＲ）的变化。正常肝组织ＡｇＮＯＲ数低于慢性肝病和癌旁肝组织（Ｐ〈０．０５），后二者低于肝细胞癌ＡｇＮＯＲ数（Ｐ〈０．０１）。不同分化程度癌组织ＡｇＮＯＲ数无差异（Ｐ〉０．０５）。１年内死亡者与生存１年以上者癌组织ＡｇＮＯＲ计数无统计学差异。结果提示，ＡｇＮＯＲ计数有助于鉴别良恶性肝病，对肝癌预后的判断无意义。     

细胞核仁组成区嗜银蛋白检测对良恶性胸腔积液的鉴别诊断 …

目的 探讨核仁组成嗜银蛋白检测在良性胸腔积液间皮细胞和恶性胸腔积液癌细胞临别诊断中的价值。方法 对５０例恶怀、３０例良性胸腔积液鹗２的胸腔积液细胞涂片行ＡｇＮＯｒ染色，观察良性胸腔积液间皮细胞和恶性胸积液癌细胞核内的ＡｇＮＯＲ数目和形态。另观察６例临床疑诊恶性胸腔积液而常规脱落细胞检查阴性者胸积液细胞核内的ＡｇＮＯＲ数目和形态。结果 恶性组癌细胞平均每核ＡｇＮＯＲ数显著高于良性组间皮细胞；恶性组癌     

大肠癌旁粘膜核仁组成区嗜银蛋白及癌胚抗原表达的研究

应用胶质银染技术及免疫组化法对31例大肠癌旁粘膜进行核仁组成区嗜银蛋白（AgNOR)形态学定量研究及癌胚抗原（CEA）检测，结果表明：从癌周组织，癌旁组织到癌组织，AgNOR计数逐渐增高（P＜0．01）；CEA阳性表达亦逐渐增强（P＜0．01）。因此，支持大肠癌发生过程中移行粘膜理论。对AgNOR计数高，CEA表达阳性的病例追踪观察可望发现早期大肠癌。     

骨髓细胞嗜银蛋白染色的方法及实用价值

AgNOR染色是一种较新的染色技术,主要应用于恶性肿瘤细胞学诊断。近年来,各国学者在这方面进行了大量研究工作,特别是在痰徐片、胸腹水、宫颈刮片、各种肿块穿刺物涂片等,应用AgNOR染色,鉴别肿瘤的良恶性、肿瘤的分型、癌前病变的检测和预后等,具有一定的诊断价值。近1年里,我们对正常骨髓细胞及急性、慢性白血病患者的骨细胞进行了AgNOR染色,并进行了分析,现报告如下。     

肝细胞肝癌癌组织及癌旁肝组织中HCVNS5蛋白的检测

为了解肝细胞肝癌（ＨＣＣ）患者丙型肝炎病毒（ＨＣＶ）感染状况，应用免疫组织化学方法以单克隆鼠抗－ＨＣＶＮＳ５检测了一组ＨＣＣ患者癌组织和癌旁肝组织中ＨＣＶ表达情况。结果：６４例ＨＣＣ患者癌旁肝组织中，共检出ＨＣＶＮＳ５阳性３０例（４６．９），阳性信号位于癌细胞和癌直细胞胞浆中，阳性细胞多呈弥漫或簇状分布；３０例ＨＣＶＮＳ５呈阳性的标本中，７例只于癌直细胞中检出，１２例只存在于癌组织中，另１１例癌旁     

Juvenile Hepatocellular Carcinoma in a Healthy Liver

Primary hepatocellular carcinoma (HCC) in patients ＜30 years old is extremely rare. In younger patients, HCC develops against a background of persistent hepatitis B virus infection. We herein report a 23-year-old woman with HCC with all-negative hepatitis virus markers developing in an apparently healthy liver. Imaging studies showed a 50-mm hypervascular mass in segment 4 of the left liver lobe, compatible with HCC. The patient underwent surgical resection. A histological examination showed the presence of poorly differentiated HCC. The patient was diagnosed with HCC developing in a healthy liver. This is an extremely rare case of non-B non-C HCC.     

血管内皮细胞生长因子诱导肝癌转移的实验研究

目的研究血管内皮细胞生长因子(Vascular Endottheial Growth Factor,VEGF)与肝癌细胞转移的相互关系。方法运用Bodyen Chamber膜侵袭培养系统培养VEGF诱导的肝癌细胞株HepG_2,培养结束后,羊膜进行苏木精染色,计算穿过羊膜的下室肝癌细胞数以及停留在羊膜内的肝癌细胞数。结果用VEGF1ng/ml、5ng/ml、10ng/ml培养5小时,下室浸润的肝癌细胞数分别为为20、64、38×10~4/ml,分别高于对照组5×10~4/ml,P<0.05或0.015ng/mlVEGF培养5小时羊膜中浸润的肝癌细胞数为45个与对照组5个比较。差异有非常显著性,P<0.01。结论 VEGF可以诱导肝癌细胞转移,最佳浓度为5ng/ml,最适培养时间为2-5小时,VEGF诱导肝癌细胞转移机制与增强肿瘤细胞的迁移能力密切相关。     

Ectopic Hepatocellular Carcinoma of the Diaphragm

Ectopic hepatocellular carcinoma (HCC) is very rarely reported. It may occur at various sites. To the best of our knowledge, only one case of ectopic HCC of the diaphragm has been reported. We present another such case with invasion to the lung. Subtotal resection of the left hemidiaphragm, wedge resection of the lung (left lower lobe), and splenectomy were undertaken. Postoperative course was unremarkable; the patient received two courses of adjuvant chemotherapy with cisplatin, VP-16, and bleomycin 1 month later. Follow-up computed tomography and ultrasound were performed 8 months later; there was no local recurrence or distal metastasis.     

Hepatocellular Carcinoma in Patients with Acute Intermittent Porphyria

ABSTRACT In a retrospective study over a 20-year period we found in the Umeå region in Sweden 11 patients (7 women and 4 men, mean age 67 years) with both hepatocellular carcinoma and acute intermittent porphyria. This coincidence was highly significant. Concomitant existence of portal cirrhosis of the liver was demonstrated in those 5 patients in whom it could be examined.     

Risk Factors for Hepatocellular Carcinoma in Patients with Cirrhosis

Recent research suggests an increase in the incidence of hepatocellular carcinoma (HCC) in the United States, which may be related to an upsurge in the sequelae of chronic liver disease from hepatitis C virus. In addition to factors related to the underlying etiology of liver disease, a number of host factors such as age, gender, and ethnic background may be associated with this increased risk. The aim of this study was to evaluate a number of potential risk factors for HCC in patients with cirrhosis. Patients with biopsy proven HCC were identified from our pathology and cancer registry databases. All those without histologic or clinical cirrhosis and non-HCC hepatic malignancies were excluded. Cirrhotic patients without HCC were also selected from the Cleveland Clinic unified transplant database and were designated controls. Extensive clinicodemographic data were obtained from the databases and chart reviews. When available, paraffin-embedded liver biopsy blocks were obtained for HFE gene analysis. Univariate comparisons were made with chi-square and Fisher's exact test and multivariate analysis was carried out with logistic regression. A total of 760 patients were included in this study, 244 documented cases of HCC and 516 cirrhotic controls without HCC. Patients' age (RR = 3.1 [2.6-3.8]; P < 0.0001), male gender (RR = 3.4 [2.3-5.1]; P < 0.0001), African-American ethnicity (RR = 3.1 [1.6-5.8]; P = 0.0005), and other non-Caucasian ethnicity (RR = 6.9 [3.2-14.4]; P < 0.0001) were independently associated with HCC. Restricting the analysis to HCV-related cirrhosis, the same risk factors remained independently associated with HCC: age (decade; RR = 2.3 [1.6-3.4]; P < 0.0001), male gender (RR = 2.9 [1.2-7.0]; P = 0.02), African-American ethnicity (RR = 3.1 [1.3-7.4]; P = 0.009), and other non-Caucasian ethnicity (RR = 15.8 [1.9-134]; P = 0.01). Iron studies did not reveal an increased risk for iron overload or HFE mutation. Male gender, advancing age, and non-Caucasian ethnic background are independently associated with HCC.     

Risk of Hepatocellular Carcinoma in Patients with Chronic Hepatitis B Virus Infection

Abstract

Objective. To determine the risk factors for the occurrence of hepatocellular carcinoma (HCC) in patients with hepatitis B virus (HBV) infection. Material and methods. A total of 620 patients who tested positive for hepatitis B surface antigen and were referred to Chiba University Hospital between February 1985 and March 2008 were included in the study and the following characteristics were analyzed: age, gender, status of hepatitis B e antigen, alanine aminotransferase level, HBV DNA level, and number of platelets (PLTs). Results. HCC was detected in 30 cases during the follow-up period (5.4 ± 5.1 years). Multivariate analysis revealed that age >?40 years [compared with patients aged <?40 years; odds ratio (OR) = 4.28; 95% confidence interval (CI) = 1.68–10.9] and PLT level <?206,000/μl (compared with patients with a higher PLT level; OR = 8.50; 95% CI = 1.98–36.2) were predictive factors for HCC occurrence. In patients aged >?40 years, the HBV DNA level (compared with <?5.0 log copies/ml; OR = 4.22, 95% CI = 1.13–15.8) and PLT level (compared with patients with >?196,000/μl PLTs; OR = 15.6, 95% CI = 2.06–118.3) were predictive factors for HCC occurrence. Conclusions. Advanced age and low PLT level were risk factors for HCC occurrence in patients with HBV infection. In patients aged >?40 years, viral load was also a risk factor for HCC.