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1.
通过对黑曲霉(Aspergillus niger)XSY0607液体深层发酵柠檬酸的培养基优化实验,得出如下结论:通因实验得出黑曲霉XSY0607发酵柠檬酸的最佳碳源为水稻秸秆、最佳有机氮源为蛋白胨、最佳无机氮源NH4NO3、最佳生长因子为牛肉膏。通过Plackett-Burman实验设计得到黑曲霉XSY0607发酵柠檬素的最佳培养基配方为:水稻秸秆粉6%、蛋白胨4.5%、NH4NO30.5%、牛肉膏0.75%、KH2PO40.15%、Mg SO40.045%和Fe SO4·7H2O 0.0015%。同时通过极差分析还可得出黑曲霉XSY0607发酵培养基中不同成分对柠檬酸发酵的影响顺序为水稻秸秆蛋白胨NH4NO3KH2PO4Mg SO4Fe SO4·7H2O牛肉膏。使用优化后的培养基进行柠檬酸发酵实验,柠檬酸产量为83.6mg/m L,较优化前产量提高了31.83%。  相似文献   

2.
通过对黑曲霉(Aspergillus niger)XSY0607液体深层发酵柠檬酸的培养基优化试验:通因试验得出黑曲霉XSY0607发酵柠檬酸的最佳碳源为水稻秸秆、最佳有机氮源为蛋白胨、得出如下结论最佳无机氮源NH4NO3、最佳生长因子为牛肉膏。通过Plackett-Burman试验设计得到黑曲霉XSY0607发酵柠檬素的最佳培养基配方为(%):水稻秸秆粉6%、蛋白胨4.5%、NH4NO 30.5%、牛肉膏0.75%、KH2PO4 0.15%、Mg SO44.5×10-3和Fe SO4·7H2O 1.5×10-5。同时通过极差分析还可得出黑曲霉XSY0607发酵培养基中不同成分对柠檬酸发酵的影响顺序为水稻秸秆蛋白胨NH4NO3KH2PO4Mg SO4Fe SO4·7H2O牛肉膏。  相似文献   

3.
利用蛋白质交联-絮凝沉淀性能测定方法,从土壤中分离得到1株高产谷氨酰胺转氨酶(microbial transglutaminase,MTG)的菌株,命名为HF-82,初步确定为链霉菌属。单因素试验确定最适产酶氮源和碳源分别是蛋白胨和葡萄糖,正交试验确定最适发酵培养基为(g/L):葡萄糖25.0,蛋白胨20.0,酵母提取物5.0,Mg SO4·7 H2O 2.0,K2HPO4·3 H2O 2.0,Na H2PO42.0,Ca CO33.0,p H7.0。此发酵工艺条件下,MTG的酶活可达到0.53 U/m L。  相似文献   

4.
在已筛选出产海藻糖合酶菌株———恶臭假单胞杆菌的基础上 ,研究了该菌株最佳发酵培养基。恶臭假单胞杆菌H76的最适碳源为麦芽糖和葡萄糖 ,最适氮源为蛋白胨和酵母粉 ,无机盐为MgSO4 ·7H2 O ;该菌株产酶最佳培养基配方为 :麦芽糖 3 % ,葡萄糖 3 % ,Mg SO4 7H2 O 0 2 % ,蛋白胨 2 % ,酵母粉 0 7%。  相似文献   

5.
对从大白菜叶片上分离得到的一株乳酸乳球菌乳酸亚种T0625产乳酸链球菌素(Nisin)的适宜培养条件进行了研究.采用单因素分析法首先确定了T0625菌株在改良的M17G培养基中产Nisin的最适碳源、氮源、金属离子的种类,即:碳源为葡萄糖;氮源为蛋白胨、酵母膏和牛肉膏组成的混合氮源;金属离子为Mg2 .并确定了各成分的最适浓度.之后确定了装液量、接种量、起始pH和培养温度的最适指标.最终通过正交试验的方法,确定T0625产Nisin的最佳营养条件:葡萄糖2%、蛋白胨4%、酵母膏2%、牛肉膏1%、MgSO4·7H2O 0.003 mol/L、VC0.05%.T0625产Nisin的最佳发酵条件:装液量250 mL(250 mL三角瓶)、接种量7%、起始pH 6.5、35℃下发酵培养12 h.  相似文献   

6.
基于胞外多糖和菌丝生物量的香菇发酵培养基优化   总被引:1,自引:0,他引:1  
为研究液体发酵培养基对香菇胞外多糖和菌丝生物量的影响,以秦巴山区香菇808菌株为试材,采用Plackett-Burman设计实验、最陡爬坡实验和响应曲面法对其液体发酵培养基的碳源、氮源和其它营养物质进行优化。结果表明,香菇胞外多糖发酵培养基的最佳组合是(g/500 m L):蔗糖7.18,玉米粉15.00,麦麸14.05,酵母膏0.35,KH2PO40.50,Mg SO4·7H2O 0.50,p H自然,胞外多糖实测值为0.967 g/500 m L;香菇菌丝生物量发酵培养基的最佳组合是(g/500 m L):蔗糖7.18,玉米粉15.00,麦麸14.05,酵母膏0.35,KH2PO40.75,Mg SO4·7H2O 0.50,p H自然,菌丝生物量实测值为28.146 g/500 m L。优化后的香菇胞外多糖产量和菌丝生物量较优化前分别提高12.44%和11.00%。此研究结果可为香菇液体发酵的中试生产提供理论依据。  相似文献   

7.
杨丽 《广西轻工业》2015,(4):14-15,19
以一株Ε-环状糊精葡萄糖基转移酶高产菌株YR为研究对象,对其发酵条件及酶学特性进行初步研究。对该菌株发酵培养基的碳源、氮源和p H进行单因子分析,并对该3个因素进行正交实验确定该菌的最佳发酵培养基配方为:0.3%马铃薯淀粉,0.5%酵母膏,0.13%K2HPO4?3H2O,0.5%Na2CO3,0.02%Mg SO4?7H2O,p H为9.0。在该条件下,菌株YR产Ε-CGTase的酶活由优化前1620.28U/m L提高到4456.34U/m L,比出发菌酶活提高了2.75倍。  相似文献   

8.
本文以钝齿棒杆菌诱变菌为实验菌株,采用摇瓶发酵的方式,研究了培养基组分(碳源、有机氮源、无机氮源及无机盐离子)与发酵培养条件(温度、接种量、初始p H及发酵液体积)对菌种产L-精氨酸的产量的影响。结果表明,该菌产L-精氨酸发酵培养基的最佳组分为:葡萄糖12%、硫酸铵4.5%、玉米浆2.5%、KH2PO40.005%、Mg SO4·7H2O0.01%、Fe Cl3·6H2O 0.01%、Mn SO4·H2O 0.05%及碳酸钙3%;最佳发酵条件是:培养温度为30℃、接种量为10%、初始p H为7.0及装液量为15m L/250m L。该菌以最优结果发酵,L-精氨酸的产量较基本培养基提高27.7%,达到了11.23g/L。  相似文献   

9.
鉴定一株从菊芋根际土壤中分离出的产外切型菊粉酶活力较高的菌株C-56。通过16S r DNA序列分析构建系统发育树,初步确定菌株的分类地位,利用单因素实验和田口方法优化培养基配方。实验发现菌株C-56属于伯克霍尔德氏菌属(Burkholderia),单因素实验确定菌株产酶的最佳碳源、氮源、无机盐分别为菊粉、酵母膏、Mg SO4·7H2O。应用田口方法优化菌株的培养基,统计学分析发现菊粉对菌株产酶的影响最大,最佳培养基配方为菊粉50 g/L,酵母浸粉20 g/L,Mg SO4·7H2O 6 g/L,p H6.0。在最佳条件下获得的菊粉酶活力为(29.34±1.95)U/m L,比初始菊粉酶酶活力(6.25±0.84)U/m L提高了3.69倍。  相似文献   

10.
以本实验室筛选到产纤溶酶的粪肠球菌EF608为发酵菌株,通过研究得到该菌株产纤溶酶的最佳工艺条件为:葡萄糖2%,蛋白胨0.75%,牛肉膏0.75%,酵母提取物0.5%,K2HPO4·3H2O1%,NaCl0.2%,MgSO4·7H2O0.02%,初始pH7.5,接种量为6%,装液量为15%,37℃培养10h,发酵液纤溶酶活性达到1833IU/mL。  相似文献   

11.
Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.  相似文献   

12.
The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.  相似文献   

13.
The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.  相似文献   

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16.
This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.  相似文献   

17.
<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the  相似文献   

18.
《印刷工业》2014,(11):95-95
According to Printing and Printing Equipment Industries Association of China(PEIAC)'s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.  相似文献   

19.
《印刷工业》2014,(8):103-103
正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press  相似文献   

20.
《印刷工业》2014,(12):102-103
Sponsored by Printing and Printing Equipment Industries Association of China (PEIAC) and organized by Print China magazine, the Seventeenth Beijing International Printing Information Conference (INFOPRINT 2014) was successfully held on 11th Dec. 2014 at Dongguan Exhibition International Hotel.  相似文献   

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