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1.
Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography/nanoelectrospray ionization‐mass spectrometry 下载免费PDF全文
Sadamu Kurono Yuka Kaneko Nariaki Matsuura Haruki Oishi Shinzaburo Noguchi Seung Jin Kim Yasuhiro Tamaki Takao Aikawa Yoshikazu Kotsuma Hideo Inaji Shuji Matsuura 《Proteomics. Clinical applications》2016,10(5):605-613
2.
Thierolf M Hagmann ML Pfeffer M Berntenis N Wild N Roeßler M Palme S Karl J Bodenmüller H Rüschoff J Rossol S Rohr G Rösch W Friess H Eickhoff A Jauch KW Langen H Zolg W Tacke M 《Proteomics. Clinical applications》2008,2(1):11-22
The aim of this study was to characterize the proteome of normal and malignant colonic tissue. We previously studied the colon proteome using 2‐DE and MALDI‐MS and identified 734 proteins (Roeßler, M., Rollinger, W., Palme S., Hagmann, M.‐L., et al.., Clin. Cancer Res. 2005, 11, 6550–6557). Here we report the identification of additional colon proteins from the same set of tissue samples using a complementary nano‐flow 2‐D‐LC‐ESI‐MS. In total, 484 proteins were identified in colon. Of these, 252 had also been identified by the 2‐DE/MALDI‐MS approach, whereas 232 proteins were unique to the 2‐D‐LC‐ESI‐MS analysis. Comparing protein expression in neoplastic and normal colon tissue indicated elevated expression of several proteins in colorectal cancer, among them the well established tumor marker carcinoembryonic antigen, as well as calnexin, 40S ribosomal protein S15a, serpin H1, and S100A12. Overexpression of these proteins was confirmed by immunoblotting. Serum levels of S100A12 were determined by ELISA and were found to be strongly elevated in colorectal cancer patients compared to healthy individuals. We conclude, that 2‐D‐LC‐ESI‐MS is a powerful approach to identify and compare protein profiles of tissue samples, that it is complementary to 2‐DE/MALDI‐MS approaches and has the potential to identify novel biomarkers. 相似文献
3.
Quantification by nano liquid chromatography parallel reaction monitoring mass spectrometry of human apolipoprotein A‐I,apolipoprotein B,and hemoglobin A1c in dried blood spots 下载免费PDF全文
Clark M. Henderson James G. Bollinger Jessica O. Becker Jennifer M. Wallace Thomas J. Laha Michael J. MacCoss Andrew N. Hoofnagle 《Proteomics. Clinical applications》2017,11(7-8)
4.
Zhang D Tai LK Wong LL Putti TC Sethi SK Teh M Koay ES 《Proteomics. Clinical applications》2008,2(1):99-107
The human epidermal growth factor receptor type 2 (HER‐2/neu) oncoprotein is overexpressed in about 30% of breast cancers and associates with metastatic phenotypes of breast tumours. Dissecting the HER‐2/neu‐modulated molecules in cancer will be helpful in elucidating the underlying molecular mechanisms of HER‐2/neu‐driven tumourigenesis. We investigated the differential proteome profiles between microdissected HER‐2/neu‐positive and ‐negative tumours and unambiguously identified 21 proteins with diverse biological functions by peptide sequencing and NCBInr database interrogation. Six proteins were up‐regulated whereas 15 were down‐regulated in the HER‐2/neu‐positive tumours. Differential expressions of heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1), 78 kDa glucose‐regulated protein (GRP78/Bip) and Raf‐1 kinase inhibitor protein (RKIP), which have not been previously reported as being linked to HER‐2/neu signalling, were further verified. Immunohistochemical staining on tissue microarray sections demonstrated a positive correlation of hnRNP H1 (p = 0.008) and negative correlations of GRP78 and RKIP (p = 0.018 and 0.013, respectively) with HER‐2/neu. Heregulin α1 enhanced hnRNP H1, but reduced GRP78 and RKIP expression in BT474 cells in a dose‐dependent manner, providing evidence of crosstalk between HER‐2/neu signalling and these modulators. Our studies have identified novel modulators that are likely to be intricately involved in HER‐2/neu‐driven tumour proliferation, invasion and metastasis. 相似文献
5.
The 88‐kDa Eales’ protein in serum is a complex of haptoglobin,complement C3, and galectin‐1 as identified by liquid chromatography coupled mass spectrometry 下载免费PDF全文
Saravanan Ramanujam Bharathselvi Muthuvel Babu Aravind C. Jyothirmay Biswas N. Sulochana Konerirajapuram 《Proteomics. Clinical applications》2017,11(1-2)