药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

Abstract Objectives To study the effect of lethal administration of drug in intravenous injection on renal endothelin (ET) and nitric oxide synthetase(NOS).Methods The technique of S - P immunohistochemical st - aining was used to study the expression of ET1 and induced NOS (iNOS) in quantity among the different storage time in living group,lethal trauma group and d lethal administration of drug in intravenous injection.Results The result showed that in the glomeralus and renal proximal tubule the expression of iNOS increased significantly(P0.05).Conclusions The expression of iNOS and ET1 is positive in the glomcrulus and re - nal proximal tubule of the kidney stored in the irrigating fluid.The expression of iNOS decreased while ET1 in - creased significantly in the experimental group of lethal administration of drug in intravenous injection.     

药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

Abstract Objectives To study the effect of lethal administration of drug in intravenous injection on renal endothelin (ET) and nitric oxide synthetase(NOS).Methods The technique of S - P immunohistochemical st - aining was used to study the expression of ET1 and induced NOS (iNOS) in quantity among the different storage time in living group,lethal trauma group and d lethal administration of drug in intravenous injection.Results The result showed that in the glomeralus and renal proximal tubule the expression of iNOS increased significantly(P0.05).Conclusions The expression of iNOS and ET1 is positive in the glomcrulus and re - nal proximal tubule of the kidney stored in the irrigating fluid.The expression of iNOS decreased while ET1 in - creased significantly in the experimental group of lethal administration of drug in intravenous injection.     

药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

Abstract Objectives To study the effect of lethal administration of drug in intravenous injection on renal endothelin (ET) and nitric oxide synthetase(NOS).Methods The technique of S - P immunohistochemical st - aining was used to study the expression of ET1 and induced NOS (iNOS) in quantity among the different storage time in living group,lethal trauma group and d lethal administration of drug in intravenous injection.Results The result showed that in the glomeralus and renal proximal tubule the expression of iNOS increased significantly(P0.05).Conclusions The expression of iNOS and ET1 is positive in the glomcrulus and re - nal proximal tubule of the kidney stored in the irrigating fluid.The expression of iNOS decreased while ET1 in - creased significantly in the experimental group of lethal administration of drug in intravenous injection.     

药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

Abstract Objectives To study the effect of lethal administration of drug in intravenous injection on renal endothelin (ET) and nitric oxide synthetase(NOS).Methods The technique of S - P immunohistochemical st - aining was used to study the expression of ET1 and induced NOS (iNOS) in quantity among the different storage time in living group,lethal trauma group and d lethal administration of drug in intravenous injection.Results The result showed that in the glomeralus and renal proximal tubule the expression of iNOS increased significantly(P0.05).Conclusions The expression of iNOS and ET1 is positive in the glomcrulus and re - nal proximal tubule of the kidney stored in the irrigating fluid.The expression of iNOS decreased while ET1 in - creased significantly in the experimental group of lethal administration of drug in intravenous injection.     

药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

Abstract Objectives To study the effect of lethal administration of drug in intravenous injection on renal endothelin (ET) and nitric oxide synthetase(NOS).Methods The technique of S - P immunohistochemical st - aining was used to study the expression of ET1 and induced NOS (iNOS) in quantity among the different storage time in living group,lethal trauma group and d lethal administration of drug in intravenous injection.Results The result showed that in the glomeralus and renal proximal tubule the expression of iNOS increased significantly(P0.05).Conclusions The expression of iNOS and ET1 is positive in the glomcrulus and re - nal proximal tubule of the kidney stored in the irrigating fluid.The expression of iNOS decreased while ET1 in - creased significantly in the experimental group of lethal administration of drug in intravenous injection.     

药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

目的 探讨药物静脉注射致死大鼠肾脏内皮素和一氧化氮释放的影响.方法 采用过氧化物酶标记的链霉卵白素染色法(S - P法),对活体组、外伤致死组、药物联合静脉注射致死组不同保存时间大鼠肾脏标本的ET - 1、iNOS 阳性表达情况进行定最分析.结果 各实验组的不同保存时间,在肾小球及近曲小管均有不同程度的ET - 1 和 iNOS 的阳性表达;同一保存时间药物联合静脉注射致死组较活体组、外伤致死组在肾小球和近曲小管,iNOS 的阳性表达率均低,ET - 1 的阳性表达率均高,差异均有显著性(P<0.01);ET - 1 和iNOS 在同一实验组不同保存时间的阳性表达率不同,由0 小时到8 小时逐渐增高,在8小时达高峰并持续到12 小时,但差异无显著性(P>0.05).结论 灌注液内低温保存的肾脏,在肾小球和近曲小管均出现ET - 1 和iNOS 的阳性表达;药物联合静脉注射致死使iNOS 的阳性表达率显著性下降和ET- 1 阳性表达显著性升高.     

药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

Abstract Objectives To study the effect of lethal administration of drug in intravenous injection on renal endothelin (ET) and nitric oxide synthetase(NOS).Methods The technique of S - P immunohistochemical st - aining was used to study the expression of ET1 and induced NOS (iNOS) in quantity among the different storage time in living group,lethal trauma group and d lethal administration of drug in intravenous injection.Results The result showed that in the glomeralus and renal proximal tubule the expression of iNOS increased significantly(P0.05).Conclusions The expression of iNOS and ET1 is positive in the glomcrulus and re - nal proximal tubule of the kidney stored in the irrigating fluid.The expression of iNOS decreased while ET1 in - creased significantly in the experimental group of lethal administration of drug in intravenous injection.     

药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

Abstract Objectives To study the effect of lethal administration of drug in intravenous injection on renal endothelin (ET) and nitric oxide synthetase(NOS).Methods The technique of S - P immunohistochemical st - aining was used to study the expression of ET1 and induced NOS (iNOS) in quantity among the different storage time in living group,lethal trauma group and d lethal administration of drug in intravenous injection.Results The result showed that in the glomeralus and renal proximal tubule the expression of iNOS increased significantly(P0.05).Conclusions The expression of iNOS and ET1 is positive in the glomcrulus and re - nal proximal tubule of the kidney stored in the irrigating fluid.The expression of iNOS decreased while ET1 in - creased significantly in the experimental group of lethal administration of drug in intravenous injection.     

药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

Abstract Objectives To study the effect of lethal administration of drug in intravenous injection on renal endothelin (ET) and nitric oxide synthetase(NOS).Methods The technique of S - P immunohistochemical st - aining was used to study the expression of ET1 and induced NOS (iNOS) in quantity among the different storage time in living group,lethal trauma group and d lethal administration of drug in intravenous injection.Results The result showed that in the glomeralus and renal proximal tubule the expression of iNOS increased significantly(P0.05).Conclusions The expression of iNOS and ET1 is positive in the glomcrulus and re - nal proximal tubule of the kidney stored in the irrigating fluid.The expression of iNOS decreased while ET1 in - creased significantly in the experimental group of lethal administration of drug in intravenous injection.     

药物静脉注射致死对大鼠肾脏内皮素影响的实验研究

Abstract Objectives To study the effect of lethal administration of drug in intravenous injection on renal endothelin (ET) and nitric oxide synthetase(NOS).Methods The technique of S - P immunohistochemical st - aining was used to study the expression of ET1 and induced NOS (iNOS) in quantity among the different storage time in living group,lethal trauma group and d lethal administration of drug in intravenous injection.Results The result showed that in the glomeralus and renal proximal tubule the expression of iNOS increased significantly(P0.05).Conclusions The expression of iNOS and ET1 is positive in the glomcrulus and re - nal proximal tubule of the kidney stored in the irrigating fluid.The expression of iNOS decreased while ET1 in - creased significantly in the experimental group of lethal administration of drug in intravenous injection.     

水蛭素对大鼠随意型皮瓣存活的影响

目的 研究水蛭素对大鼠背部超长随意型皮瓣存活的影响.方法 采用改良大鼠"McFarlane flap"模型,将实验动物随机分为水蛭素实验组(水蛭素组)和生理盐水对照组(生理盐水组),水蛭素组局部注射3 ml(30 ATU)水蛭素,生理盐水组则注射3 ml生理盐水,连续注射7 d后分别检测两组皮瓣的存活面积百分比,并取皮瓣近、中、远段(即Ⅰ、Ⅱ、Ⅲ区)组织做光镜观察,免疫组化法检测血管内皮生长因子(VEGF)和碱性成纤维细胞因子(bFGF)的表达.结果 术后7 d,水蛭素组皮瓣的存活面积百分比为(69.52±3.23)%,生理盐水组为(50.36±2.37)%,水蛭素组显著高于生理盐水组,差异有统计学意义(P＜0.01);水蛭素组皮瓣坏死与存活并存的Ⅱ区,组织水肿、炎性细胞浸润情况明显比生理盐水组轻.水蛭素和生理盐水组皮瓣Ⅱ区的新生血管计数分别为(28.24±4.23)个/mm2和(17.45±5.43)个/mm2,两组比较差异有统计学意义(P＜0.05).通过计算累积吸光度A值(IA),得到水蛭素和生理盐水组VEGF阳性量分别为9262.23±896.99和4938.05±1623.67,bFGF阳性量分别为5122.83±1176.12和2779.45±472.00,水蛭组VEGF及bFGF的表达均高于生理盐水组,差异均有统计学意义(P＜0.01).结论 水蛭素可能通过体内一系列复杂的调控通路,最终增加VEGF、bFGF表达,促进皮瓣新生血管增生,改善皮瓣血供,减轻炎性反应,降低缺血皮瓣的坏死率,从而提高大鼠随意型皮瓣的存活.

Abstract：

Objective To investigate the effect of Hirudin on random skin flap survival in rats.Methods 24 SD rats were randomly divided into control group and experimental group. The "McFarlane flap(3 cm ×9 cm)" rat models were established on the rat dorsum. 3 ml Hirudin (30 ATU) was injected into the flap in the experimental group, while 3 ml saline in the control group. The injection was performed for 7 days. The flap survival area in the two groups was measured. The tissue samples were taken from proximal( Ⅰ ), middle( Ⅱ ) and distal( Ⅲ ) portions of flaps for histologic study. The VEGF and bFGF expression was also detected with immunohistochemistry method. Results 7 days after operation, the flap survival rate was ( 69.52 + 3.23 )% in the experimental group, while ( 50.36 ± 2.37 )% in control group,showing a significant difference between the two groups ( P ＜ 0.01 ). In the middle portion, tissue edema and infiltration of neutrophils in experimental group was markedly slighter than that in control group. The VEGF and bFGF expression and neovascularization was enhanced markedly in experimental group.Conclusions Hirudin can increase the survival of random pattern skin flaps. It may increase the VEGF,bFGF expression through a series of complex regulatory pathway. Then flap neovascularization is promoted and the flap blood supply is increased.     

三七总皂苷对5/6肾切除大鼠肾脏皮质细胞外基质积聚的影响

目的：观察三七总皂苷（PNS）对5/6肾切除大鼠肾皮质细胞外基质（ECM）积聚的影响,并探讨其肾脏保护机制。方法：将45只Wistar大鼠按体重随机取8只为正常对照组,余用5/6肾切除法建立慢性肾衰竭（CRF）动物模型。将造模成功后的大鼠随机分为模型组、百令胶囊组（简称对照组）、PNS低剂量组（低剂量组）、PNS高剂量组（高剂量组）,分别给与相应浓度和剂量的药物,实验期间测定大鼠的尿蛋白、肾功能,治疗12周后观察其肾脏病理改变,用半定量方法计算肾小球硬化指数（GSI）和肾小管损伤指数,免疫组化法检测肾小球Ⅳ型胶原（ColⅣ）、纤连蛋白（FN）和肾皮质基质金属蛋白（MMP-2）和金属蛋白酶组织抑制物（TIMP-2）蛋白表达。结果：PNS能明显减少CRF大鼠尿蛋白的排出（P〈0.05）,有一定改善肾功能的作用;PNS能减轻肾脏病理损害,减轻肾小球硬化、肾小管损伤程度;PNS能下调TIMP-2蛋白表达,增加MMP-2活性,抑制了FN、ColⅣ在肾组织的表达（P〈0.01）,减轻ECM积聚。结论：PNS可能通过减少5/6肾切除大鼠尿蛋白的排出,增加肾脏MMP-2活性,降低TIMP-2表达,增加ECM降解,减轻肾小球硬化,从而起到治疗作用。     

氟伐他汀对5/6肾切除大鼠肾脏皮质细胞外基质积聚的影响

目的观察降脂药氟伐他汀对5/6肾切除大鼠肾皮质细胞外基质(ECM)积聚的影响和探讨其降脂作用外的肾脏保护机制。方法将5/6肾切除大鼠随机分为模型组和氟伐他汀治疗组(治疗组),另设假手术组作为对照。实验期间测定尿蛋白排泄率;氟伐他汀处理(7mg·kg-1·d-1)13周后免疫组化法检测肾小球Ⅳ型胶原(ColⅣ)及纤连蛋白(FN)蛋白表达;酶谱法检测肾皮质基质金属蛋白酶(MMP)-2活性;免疫印迹检测金属蛋白酶组织抑制物(TIMP)-2蛋白表达。结果与假手术组相比,模型组大鼠尿蛋白排泄、肾小球Ⅳ型胶原和FN表达均明显增加(均为P<0.01)。模型组大鼠肾皮质MMP-2活性较假手术组明显降低(P<0.01),而TIMP-2蛋白表达明显上调(P<0.01)。氟伐他汀治疗后这些改变均明显好转。结论氟伐他汀可能通过增加5/6肾切除大鼠肾脏MMP-2活性和降低TIMP-2表达,从而能增加细胞外基质降解和减轻肾小球细胞外基质积聚。     

栽培桑黄对FSGS肾病大鼠肾功能及肾纤维化机制研究

目的:研究栽培桑黄对局灶节段肾小球硬化(focal stage glomerulosclerosis,FSGS)肾病大鼠早期肾损伤的干预作用及其机制.方法:采用单侧肾切除合并尾静脉注射阿霉素构建FSGS大鼠模型,分别用人工桑黄水煎剂及水提物进行干预治疗.生化检测尿蛋白、血肌酐、尿素氮、血脂及其他指标,Masson染色观...     

益肾活血方抑制足细胞转分化减缓FSGS模型大鼠疾病进展的实验研究

目的:通过观察益肾活血方对局灶节段性肾小球硬化模型大鼠尿蛋白,肾组织结构,足细胞特异性蛋白WT1、nephrin,转分化相关指标α-SMA的影响。探讨益肾活血方对局灶节段性肾小球硬化的调控机制。方法:7～8周龄雄性Sprague Dawley大鼠,尾静脉注射多柔比星(4 mg·kg~(-1)·次~(-1)) 2次,制备FSGS模型。成模后,按24 h尿蛋白定量随机分为模型组、贝那普利组(5 mg/kg)、益肾活血方高剂量组(2 g/kg)、益肾活血方低剂量组(1 g/kg),每组9只;选择另外10只同周龄大鼠为正常组。连续给药8周。在实验结束时,检测体重、24 h尿蛋白定量(24 h U-Pr)、血肌酐、尿素氮;行肾脏组织HE、PAS染色; Western blot法检测WT1、nephrin、α-SMA蛋白表达。结果:与模型组相比,高剂量组24 h U-Pr显著降低(P 0. 05),体重显著升高(P 0. 05),肾小球硬化程度显著改善(P 0. 05); WT1蛋白在高低剂量组肾组织中的表达显著升高(P 0. 05); nephrin在治疗后有增加趋势;α-SMA模型组中表达增加(P 0. 01),益肾活血方治疗后表达下调(P 0. 05)。结论:益肾活血方能够减少尿蛋白排泄,减轻肾组织病理损害,降低肾小球硬化程度;其作用机制可能与抑制足细胞转分化,保护足细胞结构和数量(nephrin、WT1),减少足细胞损伤有关。     

ECM修复兔桡骨缺损的实验研究

[目的]研究自制的兔耳廓脱细胞软骨基质（extracellular cartilage matrix,ECM）修复骨缺损的特点及机理。[方法]采用新西兰大白兔共40只,随机分A、B两组,在其两侧桡骨干中段制作5 mm的骨缺损模型。右侧作为实验侧,缺损区A组植入脱细胞软骨基质复合自体培养骨髓干细胞,B组仅植入脱细胞软骨基质,左侧为空白对照。分别于2、4、6、10周处死动物,标本行放射学及组织学检查。[结果]X线及组织切片均证实复合体在6周时已有致密骨组织形成,10周时已有新生髓腔生成。[结论]表明兔耳廓脱细胞软骨基质与自体骨髓干细胞复合体有良好的成骨能力,有引导组织再生、防止骨不连作用。     

大鼠胰十二指肠肾脏联合移植模型的实验研究

目的：为了进行胰肾联合移植基础研究，建立一种简易可靠的大鼠胰十二指肠肾脏联合移植（SPKT）模型，方法：雄性SD大鼠作同品系异体移植的供受体，受体尾静脉注射链脲霉素（STZ）50mg/kg，建立大鼠糖尿病模型，在保存液中，移植物门静脉与肾下下腔静脉作袖套式吻合；移植物肾上腹主动脉，肾上下腔静脉分别与受体肾下腹主动脉，肾下下腔静脉显微缝合吻合，移植物十二指肠与受体十二指肠端侧吻合，移植物带输尿管的膀胱片与受体膀胱吻合。结果：血管吻合时间及受体手术时间短，手术成功率为88％，移植胰腺及肾脏功能良好。结论：此模型是一种简易可靠的大鼠胰十二指肠肾脏联合移植模型。     

天然水蛭素对猪随意型皮瓣静脉淤血影响的实验研究

目的 探讨局部应用天然水蛭素对猪随意型皮瓣移植后静脉淤血的影响. 方法 6～8月龄广西巴马小型猪3只,雌雄不限,体重10～15 kg.于每只猪背两侧制备6个大小为14 cm×4 cm的随意型皮瓣,共18个皮瓣.根据术后注射药物不同,皮瓣随机分为3组(n=6).术后即刻、1、2、3 d,A组:局部注射3 mL生理盐水,作为对照组;B组:局部注射3 mL天然水蛭素20 ATU:C组:局部注射3 mL天然水蛭素40 ATU.术后1、10 d行大体观察,1、7d行组织学观察,3、7 d测定湿重/干重比值,5 d测定皮瓣表面温度,7 d皮瓣局部血流彩色超声检测,12 d测定皮瓣成活率. 结果 术后即刻,3组皮瓣均出现远端2/3范围内轻度淤血,差异无统计学意义(P＞O.05):术后1 d,A组皮瓣淤血长度明显长于B、C组(P＜0.05),B、C组间比较差异无统计学意义(P＞0.05);术后10 d,A、C组皮瓣坏死长度明显长于B组,差异有统计学意义(P＜0.05),A、C组比较差异无统计学意义(P＞0.05).组织学观察:术后1 d,B组真皮内毛细血管及微静脉红细胞淤滞现象较A组明显减轻;术后7 d,B组皮下胶原及肉芽组织增生明显优于A组.术后3 d,A、B、C组湿重/干重比值分别为3.94 4±0.14、3.43±0.14、3.60±0.19,A组与B、C组比较,差异有统计学意义(P＜0.05),B、C组间比较,差异无统计学意义(P＞0.05);术后7 d,A、B、C组湿重/干重比值分别为3.61±0.11、3.08±0.13、3.34±0.21,A组与B、C组比较,差异有统计学意义(P＜0.05),B、C组间比较,差异无统计学意义(P＞0.05).术后5 d,A、B、C组皮瓣表面温度分别为(36.64±0.70)、(38.61±0.42)和(37.50±0.46)℃,A组与B、C组比较差异有统计学意义(P＜0.05),B、C组间比较,差异无统计学意义(P＞0.05).术后7 d,皮瓣局部血流彩色超声检测示A组血流信号稀少,B组见较完整的动静脉回流信号,可见垂直于皮肤的动脉穿支,C组可见动脉血流信号.术后12 d,A、B、C组皮瓣成活率分别为45%4±7%、67%±4%、52%±4%,B、C组与A组比较,筹异有统计学意义(P＜0.05),B、C组问比较差异有统计学意义(P＜0.05). 结论 局部应用天然水蛭素对猪随意犁皮瓣移植后静脉淤血有较明显的改善作用.     

重组水蛭素对大鼠撕脱皮瓣存活的影响

目的 探讨重组水蛭素对撕脱皮瓣的保护作用及其在临床治疗撕脱皮瓣的应用前景.方法 将108只SD大鼠随机分为重组水蛭素组（A组）、低分子肝素组（B组）、生理盐水组（C组,对照组）,并形成撕脱皮瓣后原位缝合.术后即刻、12 h、24 h、48 h,给予皮瓣蒂部、距蒂部2 cm和4 cm3等分点皮下注射重组水蛭素、低分子肝素、生理盐水,于术后12 h、1d、3d、5d、7d,分别检测皮瓣远端组织内P选择素和血管内皮细胞生长因子（VEGF）含量,病理切片观察皮瓣远端组织毛细血管内炎性细胞聚集及微血栓形成情况,术后14d计算皮瓣存活率.结果 A组P选择素含量比B组、C组增加不明显,差异具有统计学意义（P＜0.05）,A组VEGF含量比B组、C组明显增加,差异具有统计学意义（P＜0.05）;术后14 d皮瓣存活率A组明显高于B组（P＜0.05）和C组（P＜0.01）,差异具有统计学意义.结论 局部注射重组水蛭素能有效抑制P选择素的生成,减少炎性细胞的聚集,阻断微血栓的形成,改善撕脱皮瓣的局部缺血、缺氧状态;减弱内皮细胞的损伤,促进VEGF的表达,刺激新生毛细血管增生,提高撕脱皮瓣的存活率.     

砷中毒对大鼠骨密度影响的实验研究

目的在慢性砷中毒实验中研究不同浓度砷对大鼠骨密度的影响。方法 Wistar大鼠40只,雌雄各半,随机分为4组,其中3组高、中、低砷染毒组分别饮用砷(As3-)浓度为34、8.5、2.125mg/L的含砷水,对照组饮用蒸馏水。饲养2、4和6个月时使用双能X线骨密度仪测量大鼠双侧股骨骨密度(BMD)。结果各染砷组组间比较:染毒2、4、6个月时骨密度比较均为高氟组≈中氟组≈低氟组≈对照组(P>0.05);各实验组组内骨密度比较:高砷组、中砷组、低砷组及对照组均为2月≈4月≈6月(P>0.05)。结论不论染砷时间长短或浓度大小砷都不会显著影响大鼠的骨密度。