葛根总黄酮的提取研究

用超声辅助提取葛根总黄酮,考察了超声功率、超声作用时间、提取温度和乙醇浓度等因素对葛根总黄酮提取率的影响。结果表明,超声提取葛根总黄酮的较佳工艺条件为:超声功率270 W,超声作用时间20 min,提取温度70℃,乙醇浓度为65%,此条件下总黄酮提取率为167.974 mg/100 g葛根粉。     

超声波法均匀设计提取葛根总黄酮的研究

采用均匀设计方法,用超声波提取葛根总黄酮,探讨最佳提取工艺条件,为生产提供理论依据。结果表明：最佳工艺条件为超声75min,超声波功率100w,粒度100～200目,40％乙醇,液料比20：1时,实际提取率为2.21％;采用超声波提取法具有提取时间短,设备简单,操作方便,适于葛根黄酮物质的提取;均匀设计在提取研究中是一种有效的实验设计方法     

脉复生的半仿生提取工艺评价

目的:对半仿生提取法制备"脉复生合剂"工艺进行优化并评价新工艺的合理性,为改进该医院制剂的生产提供实验依据。方法:以阿魏酸含量和总固形物为考察指标,采用正交实验优化半仿生提取工艺,对有效成分、稳定性及毒性进行检测以评价新工艺。结果:最佳提取工艺条件为第一煎煮时加入8倍药材量、p H为4的水,第二煎煮时加入8倍药材量、p H为7.0的水,第3煎煮时加入8倍药材量、p H为8.0的水,每煎提取2.5小时,共提取7.5小时;工艺改进后制剂稳定性优于常规水提取工艺,其最大耐受剂量大于15.0 g/kg体重,属无毒级。结论:采用半仿生提取法制备脉复生工艺合理,操作简便,适用于推广生产。     

葛根中提取总黄酮的微型实验研究

通过微型实验方法,探讨了葛根中总黄酮的提取。与常规实验相比,微型实验具有显著的优越性。     

葛根中提取总黄酮的微型实验研究

通过微型实验方法,探讨了葛根中总黄酮的提取.与常规实验相比,微型实验具有显著的优越性.     

响应面优化葛根黄酮提取工艺研究

验以葛根粉为原料、乙醇为溶剂,研究了不同提取时间、不同提取温度、不同乙醇浓度对葛根黄酮提取率的影响,在单因素实验的基础上用紫外分光光度计法进行含量测定,并进行了3因素3水平的Box-Behnken设计优化了葛根黄酮的有关提取参数,最优工艺条件为:提取时间60min、提取温度80℃、乙醇浓度60%。在最佳优化条件下葛根黄酮提取率达到极大值,葛根黄酮提取率为36.64%。该方法快速简单,结果准确,可用于葛根素提取。     

乙醇从葛根中提取黄酮动力学

The kinetics of leaching flavonoids from Pueraria Lobata with ethanol was investigated. The effects of leaching temperature, mechanical agitation rate, concentration of ethanol and feed particle size on leaching kinetics were examined. It is found that the smaller the feed particle size or the higher the leaching temperature, the higher the leaching rate. The leaching process can be described by the shrinking-core model. The apparent activation energy is 10.8kJ·mol^-1, suggesting that the leaching process is controlled by the inner diffusion. An empirical equation relating the flavonoids leaching rate constant to the feed particle size and leaching temperature was expected.     

葛根中有效成份的提取及分析

本文针对葛根中的有效成份进行了提取及分析的研究。     

大别山区葛根黄酮的微波辅助提取工艺研究

采用微波辅助提取法,研究了提取剂体积分数、提取时间、微波功率、料液比等工艺条件对大别山区葛根黄酮提取率的影响,并采用薄层色谱法对黄酮类成分进行定性分析,再利用紫外分光光度法对其定性和定量分析。结果表明:葛根黄酮提取的最佳工艺为:乙醇体积分数60%,提取时间4 min,微波功率340 W,物料比1∶50;其黄酮类化合物的母核结构多为异黄酮、二氢黄酮及二氢黄酮醇类。     

葛根中黄酮的提取与纯化研究

以葛根粉为原料,采用乙醇提取法,研究葛根中黄酮的提取及纯化工艺。采用紫外分光光度法测定黄酮标准曲线用于计算葛根中黄酮含量。在乙醇提取法提取葛根黄酮的研究中,以提取时间、料液比、乙醇体积分率、温度为考察因素,进行单因素条件研究。结果表明,最佳提取时间为1.5 h,料液比为1∶13 g/m L,乙醇体积分率为40%,温度为60℃,葛根黄酮的提取率达到2.64 mg/g。以LS-303型大孔吸附树脂为吸附剂,以葛根黄酮在该树脂上的最佳动态吸附与解吸条件为操作参数,采用树脂柱进行葛根提取液中黄酮的分离纯化,采用紫外分光光度法检验纯化效果,研究发现LS-303型树脂对葛根黄酮有良好的纯化作用。     

Subcritical water extraction of bioactive compounds from Radix Puerariae and optimization study using response surface methodology

Subcritical water extraction (SWE) of bioactive extracts from Radix Puerariae (RP) was firstly optimized by response surface methodology (RSM) and its extraction efficiency was compared with two traditional volatile isolation methods including hot water extraction (HWE) and ethanol reflux extraction (ERE). The optimal SWE parameters were as follows: extraction temperature of 141?°C, extraction time of 58?min, and liquid-solid ratio l of 23:1?mL/g. Under these conditions, yield of bioactive extracts was 28.9?±?0.2%, which was slightly higher than that obtained by ERE, and more than two-fold the value obtained by HWE respectively. Isoflavones and polysaccharide content in SWE was significantly higher than that of HWE and slight lower than that of ERE. The results indicated that SWE could be a productive and environmental friendly technique for extracting bioactive extracts from RP.     

Optimization of Microwave-Assisted Extraction of Puerarin from Radix Puerariae using Response Surface Methodology

Statistical experimentical designs were applied to optimize microwave-assisted extraction of puerarin from Radix Puerariae. The most important factors affecting the extraction procedure were determined using a Plackett-Burman design. Results indicated that the concentration of ethanol, solvent-material ratio, extraction time, and microwave power were the main factors affecting the extraction yield. These factors were further optimized using a central composite design and response surface methodology. The optimal extraction parameters were ethanol concentration of 52.36%, microwave irradiation time of 60 s, microwave power of 184.8 W and solvent-material ratio of 25:1(mL/g). The average experimental puerarin yield under the optimum conditions was found to be 11.97 mg/g, which agreed with the predicted value of 11.8 mg/g. The proposed method shows high degree of reproducibility.     

麦冬药渣中麦冬多糖的提取工艺研究

目的探索并确定从麦冬药渣中提取麦冬多糖的最佳工艺,为麦冬药渣的综合利用提供依据。方法以多糖得率为指标,通过单因素试验研究4个提取因素:提取温度、提取时间、料液比、提取次数对麦冬药渣中多糖提取效率的影响,然后,通过正交试验对提取工艺条件进行优化。结果优选出麦冬多糖的最佳提取条件为:提取温度:100℃,料液比1∶12,提取时间为120 min,提取2次;另外,与直接从生药中提取麦冬多糖相比,利用药渣提取麦冬多糖,虽多糖得率有所下降,但多糖含量却有提高。结论该工艺条件可重复,多糖得率较高,此方法使麦冬中的有效成分综合利用,减少了中药资源的浪费。     

丹参制剂的研究进展

从丹参的化学成分、药理作用和提取工艺方面进行了综述,阐述了丹参的研究现状和应用前景.     

超临界CO_2萃取赤芍中芍药苷工艺条件研究

文章运用正交试验设计方法优化超临界CO2萃取中药赤芍的萃取工艺,并利用HPLC法测定萃取物中芍药苷含量。以萃取温度、萃取时间和萃取压力为考察因素,进行3因素3水平正交试验,考察3个因素对萃取物中芍药苷含量的影响。结果表明超临界CO2萃取赤芍中芍药苷的最佳萃取工艺为:萃取压力为18 MPa,萃取温度为25℃,萃取时间为1.5 h。     

赤芍活性成分微波辅助提取工艺的响应曲面法优化研究

以水为溶剂,采用微波辅助法提取赤芍活性成分;以最大紫外吸收波长处的吸光度为评价指标,通过单因素实验、Box-Behnken设计和响应曲面法考察了提取温度、提取时间、液料比等对提取效果的影响,对赤芍的提取工艺进行了优化分析;采用毛细管电泳法对所得赤芍水提液进行分离检测,并对不同产地赤芍的活性成分含量进行了比较分析。确定赤芍活性成分的最优提取条件为:提取温度89.99℃、提取时间75.32min、液料比79.25∶1(mL∶g),在此条件下,赤芍水提液的吸光度预测值为0.3022,实测值为0.2899,表明该方法预测效果良好,可为中药活性成分的微波辅助提取提供参考。     

正交试验法探讨实验室提取沉香挥发油的最佳条件

用正交试验法对沉香挥发油的提取工艺条件进行优选,以挥发油得率和乳化比值为指标,考察影响挥发油收率的因素并得出最佳提取工艺。结果发现沉香挥发油的最佳提取工艺为药材粉碎成粗粉过10目筛,加入6倍量的水、浸泡8 h、提取8 h。此提取工艺适合实验室及小批量生产。     

Process Optimization of Ultrasonic Extraction of Puerarin Based on Support Vector Machine

In ultrasonic extraction technology, optimization of technical parameters often considers extraction medium only,without including ultrasonic parameters. This paper focuses on controlling the ultrasonic extraction process of puerarin, investigating the influence of ultrasonic parameters on extraction rate, and empirically analyzing the main components of Pueraria, i.e., isoflavone compounds. A method is presented combining orthogonal experimental design with a support vector machine and a predictivemodel is established for optimization of technical parameters. Fromthe analysiswith the predictivemodel, appropriate process parameters are achieved for higher extraction rate. With these parameters in the ultrasonic extraction of puerarin, the experimental result is satisfactory. This method is of significance to the study of extracting root-stock plant medicines.     

洗衣机用PP复合材料的研制

讨论了各组分对聚丙烯(PP)复合材料性能的影响。以高韧性PPEPS30R为基体树脂,通过高流动性M—1600和DCP改善其流动性,滑石粉增强其刚性,并用正交设计方法优化配方。