Impact of land‐use intensity on the relationships between vegetation indices,photosynthesis and biomass of intensively and extensively managed grassland fens

Vegetation indices are widely used as model inputs and for non‐destructive estimation of biomass and photosynthesis, but there have been few validation studies of the underlying relationships. To test their applicability on temperate fens and the impact of management intensity, we investigated the relationships between normalized difference vegetation index (NDVI), leaf area index (LAI), brown and green above‐ground biomass and photosynthesis potential (PP). Only the linear relationship between NDVI and PP was management independent (R² = 0·53). LAI to PP was described by a site‐specific and negative logarithmic function (R² = 0·07–0·68). The hyperbolic relationship of LAI versus NDVI showed a high residual standard error (s.e.) of 1·71–1·84 and differed between extensive and intensive meadows. Biomass and LAI correlated poorly (R² = 0·30), with high species‐specific variability. Intensive meadows had a higher ratio of LAI to biomass than extensive grasslands. The fraction of green to total biomass versus NDVI showed considerable noise (s.e. = 0·13). These relationships were relatively weak compared with results from other ecosystems. A likely explanation could be the high amount of standing litter, which was unevenly distributed within the vegetation canopy depending on the season and on the timing of cutting events. Our results show there is high uncertainty in the application of the relationships on temperate fen meadows. For reliable estimations, management intensity needs to be taken into account and several direct measurements throughout the year are required for site‐specific correction of the relationships, especially under extensive management. Using NDVI instead of LAI could reduce uncertainty in photosynthesis models.     

Proximal sensing of the seasonal variability of pasture nutritive value using multispectral radiometry

The nutritive value of pasture is an important determinant of the performance of grazing livestock. Proximal sensing of in situ pasture is a potential technique for rapid prediction of nutritive value. In this study, multispectral radiometry was used to obtain pasture spectral reflectance during different seasons (autumn, spring and summer) in 2009–2010 from commercial farms throughout New Zealand. The analytical data set (n = 420) was analysed to develop season‐specific and combined models for predicting pasture nutritive‐value parameters. The predicted parameters included crude protein (CP), acid detergent fibre (ADF), neutral detergent fibre (NDF), ash, lignin, lipid, metabolizable energy (ME) and organic matter digestibility (OMD) using a partial least squares regression analysis. The calibration models were tested by internal and external validation. The results suggested that the global models can predict the pasture nutritive value parameters (CP, ADF, NDF, lignin, ME and OMD) with moderate accuracy (0·64 ≤ r² ≤ 0·70) while ash and lipid are poorly predicted (0·33 ≤ r² ≤ 0·40). However, the season‐specific models improved the prediction accuracy, in autumn (0·73 ≤ r² ≤ 0·83) for CP, ADF, NDF and lignin; in spring (0·61 ≤ r² ≤ 0·78) for CP and ash; in summer (0·77 ≤ r² ≤ 0·80) for CP and ash, indicating a seasonal impact on spectral response.     

Optimizing legume content and forage yield of mown white clover–Italian ryegrass mixtures through nitrogen fertilization and grass row spacing

Mixed swards of white clover–grass mixtures in highly productive environments often fail to reach the minimum recommended annual clover proportion of about 0·30. This study assessed the effect on clover content and total dry matter (DM) yield of two spring N applications (0 and 45 kg N ha^?1) and two distances between drilled grass‐rows (0·18 and 0·36 m) over 3 years for mown swards of white clover–Italian ryegrass (Trifolium repens–Lolium multiflorum) in binary mixtures in northern Italy. An additional aim was to determine the advantage of association of grass–clover compared with grass and clover monocultures. On average, N fertilization of mixtures resulted in almost 9% higher total yield (P < 0·01; mean response = 18·1 kg of total DM per kg of N) but decreased the clover proportion (0·250 vs. 0·312). Wider grass‐row spacing increased clover proportion (0·327 vs. 0·234; P < 0·01) with no reduction of total DM yield. N fertilization × grass‐row spacing interaction occurred only for clover content (P < 0·01). Without N fertilization, mixtures out‐yielded clover and grass pure stands. With N fertilization, at double rate to pure grass, yields from mixtures were greater than from clover and comparable to Italian ryegrass.     

Effect of defoliation management, based on leaf stage, on perennial ryegrass (Lolium perenne L.), prairie grass (Bromus willdenowii Kunth.) and cocksfoot (Dactylis glomerata L.) under dryland conditions. 2. Nutritive value

A field experiment was undertaken between April 2003 and May 2004 in southern Tasmania, Australia, to quantify and compare changes in the nutritive value of perennial ryegrass (Lolium perenne L.), prairie grass (Bromus willdenowii Kunth.) and cocksfoot (Dactylis glomerata L.) under a defoliation regime based on stage of leaf regrowth. Defoliation interval was based on the time taken for two, three or four leaves per tiller to fully expand. At every defoliation event, samples were collected and analysed for acid‐detergent fibre (ADF), neutral‐detergent fibre (NDF) and total nitrogen (N) concentrations and to estimate metabolizable energy (ME) and digestible dry matter (DDM) concentrations. Amounts of crude protein (CP) and metabolizable energy (MJ) per hectare values were subsequently calculated. There was a significantly lower (P < 0·001) NDF concentration for perennial ryegrass compared with prairie grass and cocksfoot, and a significantly lower (P < 0·001) ADF concentration for cocksfoot compared with prairie grass and perennial ryegrass, regardless of defoliation interval. The CP concentration of cocksfoot was significantly greater (P < 0·001) compared with the CP concentrations of prairie grass and perennial ryegrass. The estimated ME concentrations in cocksfoot were high enough to satisfy the requirements of a lactating dairy cow, with defoliation at or before the four‐leaf stage maintaining ME concentrations between 10·7 and 10·9 MJ kg^?1 DM, and minimizing reproductive plant development. The ME concentrations of prairie grass (10·2–10·4 MJ kg^?1 DM) were significantly lower (P < 0·001) than for cocksfoot (as above) and perennial ryegrass (11·4–11·6 MJ kg^?1 DM) but a higher DM production per hectare resulted in prairie grass providing the greatest amounts of ME ha^?1.     

Increased concentration of water-soluble carbohydrate in perennial ryegrass (Lolium perenne L.): milk production from late-lactation dairy cows

Eight multiparous Holstein–Friesian dairy cows in late lactation were used to investigate the potential of using perennial ryegrass with a high concentration of water‐soluble carbohydrate (WSC) to increase the efficiency of milk production. After a pretreatment period on a common pasture, the cows were each given ad libitum access to one of two varieties of zero‐grazed grass continuously for 3 weeks. Treatments were: high sugar (HS), an experimental perennial ryegrass variety bred to contain high concentrations of WSC; or control, a standard variety of perennial ryegrass (cv. AberElan) containing typical concentrations of WSC. The two grass varieties were matched in terms of heading date. All animals also received 4 kg day^–1 standard dairy concentrate. Grass dry matter (DM) intake was not significantly different between treatments (11·6 vs. 10·7 kg DM day^–1; s.e.d. 0·95 for HS and control diets respectively), although DM digestibility was higher on the HS diet (0·71 vs. 0·64 g g^–1 DM; s.e.d. 0·23; P < 0·01) leading to higher digestible DM intakes for that diet. Milk yield from animals offered the HS diet was higher (15·3 vs. 12·6 kg day^–1; s.e.d. 0·87; P < 0·05) and, although milk constituent concentrations were unaffected by treatment, milk protein yields were significantly increased on the HS diet. The partitioning of feed N was significantly affected by diet, with more N from the HS diet being used for milk production (0·30 vs. 0·23 g milk N g^–1 feed N; s.e.d. 0·012; P < 0·01) and less being excreted in urine (0·25 vs. 0·35; s.e.d. 0·020; P < 0·01). In a separate experiment, using the same grasses harvested earlier in the season, the fractional rate of DM degradation, measured by in situ and gas production techniques, was higher for the HS grass than for the control. It is concluded that increased digestible DM intakes of the HS grass led to increased milk yields, whereas increased efficiency of utilization of the HS grass in the rumen resulted in the more efficient use of feed N for milk production and reduced N excretion.     

Effect of six-weekly harvests on the yield, chemical composition and dry matter degradability of Panicum maximum and Stylosanthes hamata in Nigeria

The dry matter (DM) yield and degradability of 6‐week‐old harvests of tropical forages were measured over a season. The forages were nitrogen‐fertilized Guinea grass (Panicum maximum, NFG), unfertilized Guinea grass (UFG), Verano stylo (Stylosanthes hamata,VS), a Guinea grass–Verano stylo mixture (GSM) and Guinea grass in the grass–Verano stylo mixture (GGSM). Six‐week‐old forages were made possible through a cutting regime, which produced four harvests in the growing season. The DM yields of the forages differed significantly (P < 0·001) and showed a significant reduction (P < 0·01) across the season. Crude protein and neutral‐detergent fibre concentrations were significantly (P < 0·01) different between the forages but there was no difference between harvests. The DM degradability of the forages at all harvests were significantly (P < 0·001) different with differences in the soluble fraction (a), degradable fraction (b), potential degradability (PD) and effective degradability (ED), but rate of degradability (c) did not show any significant difference between the forages. Significant (P < 0·01) differences were found between harvests for b and PD, and for the interaction between forage and harvest for b, PD and ED but were not found for the a and c fractions. Both the PD and ED values of all the forages fell with advancing harvests. Although the 6‐week‐old harvests of forage were found not to influence the characteristic reduction in yield of tropical grasses over time, it is concluded that such a management system could be used to obtain forage of relatively high nutritive value during the growing season.     

Nitrogen recovery and loss in a fertilized elephant grass pasture

Limited information is available regarding the recovery and loss of fertilizer nitrogen (N) applied to intensively managed tropical grass pastures. An experiment was carried out in Brazil to determine the fertilizer‐N recovery and ammonia volatilization loss in an elephant grass (Pennisetum purpureum, Schum.) pasture fertilized with 100 kg N ha^?1 as urea or ammonium sulphate, labelled with ¹⁵N, in late summer (LS) or in mid‐autumn (MA). Herbage mass was highest and litter mass was lowest in LS (P < 0·05). The N concentration of herbage was highest in autumn (P < 0·05) and the total N content in soil was lower in LS than in MA (P < 0·05), reflecting the high N uptake capacity of the grass. Proportionately higher ¹⁵N recovery in litter mass (P < 0·05) was observed in autumn (0·094) than in LS (0·0397) and the ¹⁵N recovery in herbage was 0·046 higher for ammonium sulphate‐fertilized pastures (P < 0·05; proportionately 0·243 for ammonium sulphate and 0·197 for urea). Around 0·60 of the fertilizer‐¹⁵N recovered was retained in soil and in non‐harvestable fractions of the plant. The NH₃ volatilization loss was higher in LS and most of the N loss occurred soon after fertilizer application. Urea and ammonium sulphate fertilizers were equally effective in sustaining herbage dry matter yield in the short term. However, the use of ammonium sulphate, rather than urea, would be preferable for LS applications when the objective is to reduce NH₃ volatilization losses.     

Carbohydrate and crude protein fractions in perennial ryegrass as affected by defoliation frequency and nitrogen application rate

The objective of this study was to evaluate the effects of defoliation frequency (either at two‐ or three‐leaf stage) and nitrogen (N) application rate (0, 75, 150, 300, 450 kg N ha^?1 year^?1) on herbage carbohydrate and crude protein (CP) fractions, and the water‐soluble carbohydrate‐to‐protein ratio (WSC:CP) in perennial ryegrass swards. Crude protein fractions were analysed according to the Cornell carbohydrate and protein system. Carbohydrate fractions were analysed by ultra‐high‐performance liquid chromatography. Sward defoliation at two‐leaf stage increased the total CP, reduced the buffer‐soluble CP fractions and decreased carbohydrate fractions of herbage (P < 0·001). The effect of defoliation frequency was less marked during early spring and autumn (P < 0·001) than for the rest of the seasons. An increase in N application rate was negatively associated with WSC, fructans and neutral detergent fibre (P < 0·001), and positively associated with CP and nitrate (N‐NO₃) contents of herbage. Nitrogen application rate did not affect CP fractions of herbage (P > 0·05). The fluctuations in CP and WSC contents of herbage resulted in lower WSC:CP ratios during early spring and autumn (0·45:1 and 0·75:1 respectively) than in late spring (1·11:1). The herbage WSC:CP ratio was greater (P < 0·001) at the three‐leaf than the two‐leaf defoliation stage and declined as the N application increased in all seasons (P < 0·001). The results of this study indicate that CP and carbohydrate fractions of herbage can be manipulated by sward defoliation frequency and N application rate. The magnitude of these effects, however, may vary with the season.     

Effect of application timing and grass height on the nitrogen fertilizer replacement value of cattle slurry applied with a trailing‐shoe application system

This study investigated the effect of using a trailing‐shoe system to apply cattle slurry, under different conditions of grass height (low [LG]: freshly cut sward [4–5 cm height] vs. high [HG]: application delayed by 7–19 d and applied to taller grass sward [4–11 cm] height) and month of application (June vs. April), on the nitrogen fertilizer replacement value (NFRV) and apparent N recovery (ANR_S) of cattle slurry applied to grassland. NFRV was calculated using two methods: (i) NFRV_N based on the apparent recovery of slurry‐N relative to that of mineral‐N fertilizer; and (ii) NFRV_DM based on DM yield. The effect of applying slurry into HG swards, relative to LG swards, decreased the DM yield by 0·47 t ha^?1 (P ≤ 0·001), N uptake by 5 kg ha^?1 (P = 0·05), ANR_S by 0·05 kg kg^?1 (P = 0·036), NFRV_N by 0·05 kg kg^?1 (P = 0·090) and NFRV_DM by 0·11 kg kg^?1 (P < 0·001). It was concluded that the main factor causing these decreases with HG, compared with LG applications, was wheel damage affecting subsequent N uptake and growth of the taller grass sward.     

Effects of systemic fungal endophytes on the performance of meadow fescue and tall fescue in mixtures with red clover

A symbiosis between grasses and systemic fungal endophytes exists in both natural and agricultural grassland communities. Our objective was to examine the effects of systemic endophytes on the competitive ability of two agronomically important grass species: meadow fescue [Festuca pratensis (Huds.) syn. Schedonorus pratensis (Huds.) P. Beauv] and tall fescue [Festuca arundinacea (Schreb.) syn. Schedonorus phoenix (Scop.)]. Plants of meadow and tall fescue were grown for 48 days in replacement series of interspecific mixture with a legume (red clover, Trifolium pratense L.) in different nutrient environments in a greenhouse. Neither of the grass species gained endophyte‐promoted competitive advantage over red clover in grass–clover mixtures. Endophyte infection increased the growth of meadow fescue monocultures by 89% compared to endophyte‐free monocultures in high‐nutrient soils, but plant competition or the cost of endophyte infection to the meadow fescue decreased the yield in resource‐limited conditions. On average, endophyte‐infected and endophyte‐free meadow fescues produced 0·15 and 0·17 g, and 0·14 and 0·14 g dry biomass per plant in mixtures with red clover in high‐ and low‐nutrient soils respectively. In contrast to meadow fescue, endophyte‐promoted growth of tall fescue monocultures was not detected. Endophyte‐infected and endophyte‐free tall fescue monocultures produced 0·76 and 0·95 g biomass per pot, respectively, in the high‐nutrient environment. Endophyte infection can increase the performance of the host grass, but the positive effects depend on the host species, the species composition and soil nutrient availability.     

Weight gains,blood parameters and faecal egg counts when meat‐goat kids were finished on alfalfa,red clover and orchardgrass pastures

The experiment was conducted in 2005–2007 to evaluate weight performance, blood parameters associated with forage nutrient‐use and anaemia from gastrointestinal nematode (GIN) infection, and faecal egg count (FEC) patterns of meat‐goat kids finished on alfalfa (Medicago sativa L.; ALF); red clover/grass mixture (Trifolium pratense L.; RCG); and orchardgrass (Dactylis glomerata L; OGR) pastures. Forage mass, crude protein (CP) and total digestible nutrients (TDN) displayed complex interactions between treatment and time (P < 0·001) across the grazing seasons. Final body weight was greater for goats finished on ALF and RCG than on OGR, except in 2006 when ALF was greater than RCG or OGR. The TDN/CP ratios in forages and blood urea nitrogen concentrations in grazing goats were highly correlated (r = 0·99; P = 0·02) and suggested that animals were wasting forage protein. Faecal egg count was variable over the grazing season each year (date and treatment × date interaction; P < 0·001), but in general, FEC indicated that goat kids grazing ALF were less affected by GIN than kids grazing RCG or OGR. Research is needed to determine whether strategic energy supplementation would improve protein‐use efficiency and resilience to parasite infection when finishing meat goats on pastures managed for high forage nutritive value.     

Seed yield variation in plains rough fescue (Festuca hallii (Vasey) Piper) populations and its relation with phenotypic characteristics and environmental factors

Plains rough fescue (Festuca hallii (Vasey) Piper) is a dominant grass in the endangered Fescue Prairie of North America. Infrequent and unpredictable seed production presents a challenge for the use of this species in restoration and rangeland seeding. The objective of this study was to compare seed yield of different plains rough fescue populations and to determine the dependence of seed yield on phenotypic characteristics. Effect of weather conditions during the floral induction and initiation period of different years of the study was also compared. In 2007, a completely randomized field plot experiment was established from eleven populations of plains rough fescue at Swift Current, SK, Canada. In 2007, 2010 and 2011, individual plant seed yield, reproductive tillers, above‐ground biomass, plant height and crown diameter were measured, and plant vigour was scored. All measured variables differed significantly (P ≤ 0·05) among populations. Four populations were identified as having higher seed yield potential. Plants in these four populations also had characteristics of good plant vigour, taller stems, more reproductive tillers and greater biomass. Seed yield increased linearly with increasing plant height, crown diameter, above‐ground biomass and number of reproductive tillers (r² ranged 0·17–0·67, P < 0·001), but number of reproductive tillers (r² = 0·53–0·67, P < 0·001) was a better predictor for selection of lines with higher seed yield. Although seed yield varied among years, populations with higher seed yield tended to produce greater amounts of seed over the period of the study.     

A new sterilization and inoculation method in silage research

The study aimed at evaluating an effective sterilization–inoculation technique to facilitate silage research on the effect of forage microflora on fermentation variables. The sterilization effect of heating at 60°C for 3 h + 103°C for 15 h was tested on samples of grass, grass–clover, white clover and maize, pre‐dried at 60°C to a dry‐matter (DM) content >900 g kg^?1. The ensilability of treated samples, reconstituted to original DM concentration (250–390 g kg^?1), was assessed by inoculation with microfloras extracted from the original samples. Microfloral inoculants were obtained by a combination of centrifugation (15 500  g for 40 min) and filtration (0·45 and 0·22 μm pore sizes) of the supernatant. The sterilization treatment effectively sterilized the forage samples but decreased water soluble carbohydrates by 49% and N buffer solubility by 22% and increased the acid detergent insoluble N proportion of total N by 53% (P < 0·05). The reconstituted silages had 18% less lactic acid, 20% less ethanol and 37% less ammonia‐N (P < 0·05), but volatile fatty acids and 2,3‐butanediol did not differ from the untreated silages (P > 0·05). Counts of lactic acid bacteria, enterobacteria, clostridia, yeasts and moulds in the two silage treatments were also similar (P > 0·05). It is concluded that, despite causing chemical and physical alterations, the sterilization–inoculation technique evaluated could be a useful tool for future studies on the effects of microflora on ensiling results.     

The effect of Calluna vulgaris cover on the performance and intake of ewes grazing hill pastures in northern Spain

The effect of the proportion of Calluna vulgaris cover on diet composition, intake and performance of sheep grazing hill vegetation communities in northern Spain is examined. A total of 591 non‐lactating Gallega ewes grazed for five consecutive grazing seasons (June to September) on replicated plots of hill pastures (1700 m.a.s.l.) composed principally of Festuca, Agrostis, Nardus and Calluna spp. but with different proportions of Calluna vulgaris cover, either 0·3 (C_0·3) or 0·7 (C_0·7) of the total area. In 1 year, twenty‐eight ewes suckling single lambs also grazed the plots. The mean stocking density over the 5 years was 8·7 ewes ha^–1. On treatment C_0·3, daily liveweight gains (33 g d^–1) of non‐lactating ewes were significantly (P < 0·001) greater than on treatment C_0·7 (12 g d^–1). Likewise in lactating ewes the difference in mean daily liveweight change was 40 g d^–1 (–5 vs. –45 g d^–1 for C_0·3 and C_0·7 treatments respectively; P < 0·001). Liveweight gains of lambs were only 80–100 g d^–1 from June to August and lambs only maintained live weight during August and September. The effect of lactational status on liveweight changes was not significant. Liveweight gains of non‐lactating ewes increased significantly (P < 0·001) from the first to the last year of the experiment on both treatments. The composition of the diet was significantly affected by treatment (P < 0·001), with a higher proportion of grass species on the C_0·3 treatment and a higher digestibility of the diet in the first half of the grazing season (P < 0·001). The proportion of C. vulgaris in the diet was significantly (P < 0·001) higher on the C_0·7 treatment and increased significantly (P < 0·001) from July to September on both treatments. There were no significant differences in the composition of the diet selected by lactating and non‐lactating ewes. The results demonstrate that on hill vegetation communities, in which the grass components (Festuca rubra, Agrostis capillaris, and Nardus stricta) cover at least 0·3 of the area and on which the preferred grass component (Festuca and Agrostis spp.) is maintained at a sward height of at least 2·5 cm, non‐lactating ewes can increase their live weight and body condition, but this increase is influenced by the proportion and quantity of species of grass in the diet, which is affected in turn by the species of grass available and their nutritive quality. However, ewes suckling lambs were not able to maintain their live weight and body condition except when Calluna cover was 0·3 and grass height was more than 3·5 cm. It is concluded that these indigenous vegetation communities can be used in sheep production systems to complement the use of improved pastures at other times of year. In particular, they can be utilized during the non‐lactating period (summer) to increase body condition before the beginning of the mating period in autumn.     

Herbage and animal responses on continuously stocked ‘Ipyporã’ and ‘Mulato II’ brachiariagrasses

New hybrid grass cultivars may enhance animal performance in forage-livestock systems if they possess traits that address edaphoclimatic challenges and pest susceptibility. The objective was to assess herbage accumulation, plant-part composition, nutritive value, and animal performance of ‘Ipyporã’ [Brachiaria ruziziensis Germ. & Evrard × B. brizantha (Hochst. ex A. Rich.) Stapf] and ‘Mulato II’ (B. ruziziensis × B. brizantha × B. decumbens Stapf) hybrids in the Brazilian Amazon biome. From May 2016 to May 2018, pastures were maintained under continuous stocking with variable stocking rates to maintain canopy height at 30-cm. Herbage accumulation (HA) was greater in Mulato II (17,370 kg dry matter [DM] ha⁻¹ year⁻¹) than Ipyporã pastures (14,930 kg DM ha⁻¹ year⁻¹) across the years. In Year 1, Mulato II had greater stocking rate (1685 vs. 1215 kg body weight [BW] ha⁻¹) and greater gain ha⁻¹ (1130 vs. 850 kg) than Ipyporã. However, in Year 2, both cultivars had similar plant and animal responses. The Year 1 ADG was similar among cultivars or seasons but in Year 2, rainy seasons had 21 and 77% greater ADG than dry seasons for Mulato II and Ipyporã, respectively. Both cultivars can contribute to the diversification of pasture-based livestock systems in humid tropical regions. Mulato II presented superior performance when soil moisture and fertility were not limiting (i.e., Year 1). However, both cultivars provide similar plant and animal responses in Year 2, offering viable alternatives for the diversification of pasture-based livestock systems in the Amazon Biome.     

Characterization for industrial purposes of the fibre anatomy of perennial ryegrass and tall fescue stem and leaf at three stages in the primary growth

Characterization of grass fibre is important in assessing its potential for industrial fibre applications. Stem and leaf sections were sampled from triplicate field plots of two grass species, perennial ryegrass (PRG) and tall fescue at three dates (monthly from 12 May) in the primary growth, and were used to (a) isolate individual fibre cells and (b) prepare transverse sections. Microscopy and image analysis software were used to determine the length and width of individual fibre cells and the proportion of lignified fibre in stem and leaf transverse sections. The length and width of individual fibre cells were greater (P < 0·001) in stem than in leaf sections, while individual fibre cell length was greater (P < 0·01) for tall fescue than PRG. Harvest date and grass species had little effect (P > 0·05) on the proportion of lignified fibre in the transverse‐sectional area. However, there was a greater (P < 0·001) proportion of lignified fibre in the transverse‐sectional area of grass stems than leaves, with tall fescue having a greater (P < 0·05) stem and leaf transverse‐sectional area and area of lignified fibre in transverse section than PRG. Tall fescue harvested at a later stage of growth, with higher proportions of stem than leaf tissue, may be more suitable for industrial fibre applications.     

Impact of spatial heterogeneity of plant species on herbage productivity,herbage quality and ewe and lamb performance of continuously stocked,perennial ryegrass–white clover swards

The benefits of white clover (Trifolium repens L.) in pastures are widely recognized. However, white clover is perceived as being unreliable due to its typically low content and spatial and temporal variability in mixed (grass‐legume) pastures. One solution to increase the clover proportion and quality of herbage available to grazing animals may be to spatially separate clover from grass within the same field. In a field experiment, perennial ryegrass (Lolium perenne L.) and white clover were sown as a mixture and compared with alternating strips of ryegrass and clover (at 1·5 and 3 m widths), or in adjacent monocultures (strips of 18 m width within a 36‐m‐wide field). Pastures were stocked by ewes and lambs for three 10‐month grazing periods. Over the 3 years of the experiment, spatial separation of grass and clover, compared with a grass–clover mixture, increased clover herbage production, although its proportion in the sward declined through time (0·49–0·54 vs 0·34 in the mixture in the first year, 0·28–0·33 vs 0·15 in the second year and 0·03–0·18 vs 0·01 in the third year). Total herbage production in the growing season in the spatially separated treatments decreased from 11384 kg DM ha^?1 in the first year to 8150 kg DM ha^?1 in the third year. Crude protein concentration of clover and grass components in the 18‐m adjacent monoculture treatment was greater than the mixture treatment for both clover (310 vs 280 g kg^?1 DM) and grass (200 vs 180 g kg^?1 DM). There was no clear benefit in liveweight gain beyond the first year in response to spatially separating grass and clover into monocultures within the same field.     

Performance of white clover/perennial ryegrass mixtures under cutting

Clover persistence in mixtures of two varieties of perennial ryegrass (Lolium perenne) with contrasting growth habits and three white clover (Trifolium repens) varieties differing in leaf sizes was evaluated at two cutting frequencies. An experiment was sown in 1991 on a clay soil. The plots received no nitrogen fertilizer. In 1992, 1993 and 1994, mixtures containing the large-leaved clover cv. Alice yielded significantly more herbage dry matter (DM) and had a higher clover content than mixtures containing cvs Gwenda and Retor. Companion grass variety did not consistently affect yield or botanical composition. Cutting at 2 t DM ha^?1 resulted in slightly higher total annual yields than cutting at 1.2 t DM ha^?1, but did not affect clover content. In 1992 the mixtures yielded, depending on cutting frequency and variety, 10·6–14·6 t DM ha^?1 and 446–599 kg ha^?1 N, whereas grass monocultures yielded only 1·2–2·0 t DM ha^?1 and 25–46 kg ha^?1 N. From 1992 to 1994 the annual mean total herbage yield of DM in the mixtures declined from 12·2 to 10·5 to 8·7 t ha^?1, the white clover yield declined from 8·7 to 6·5 to 4·1 t ha^?1 and the average clover content during the growing season declined from 71% to 61% to 46%, whereas the grass yield increased from 3·4 to 4·0 to 4·5 t ha^?1. The N yield decreased from 507 to 406 to 265 kg N ha^?1 and the apparent N fixation from 470 to 380 to 238 kg N ha^?1. Nitrate leaching losses during the winters of 1992–93 and 1994–95 were highest under mixtures with cv. Alice, but did not exceed 10 kg N ha^?1. The in vitro digestible organic matter (IVDOM) was generally higher in clover than in grass, particularly in the summer months. No differences in IVDOM were found among clover or grass varieties. The experiment will be continued to study clover persistence and the mechanisms that affect the grass/clover balance.     

The effects of incorporating small quantities of straw in grass/grass silage-based diets for dairy cows

Two studies were conducted to examine the effects of incorporating small quantities of straw in the diets of dairy cows. In Experiment 1, forty Holstein Friesian dairy cows were used in a 2 × 4 factorial design experiment, with factors examined consisting of two parities (primiparous and multiparous animals) and four levels of straw inclusion in the diet (0, 0·08, 0·16 and 0·24 of forage dry matter). The basal forage offered in this study was grass silage, and the primiparous and multiparous animals were supplemented with 9·0 and 11·0 kg concentrate d^–1 respectively. In Experiment 2, forty‐eight Holstein Friesian dairy cows were used in a 2 × 3 factorial design experiment, with factors examined consisting of two basal forage types (grass silage and zero‐grazed grass) and three levels of straw inclusion (0, 1·0 and 2·0 kg d^–1). All animals were offered 7·0 kg d^–1 of a concentrate supplement. Both experiments were partially balanced changeover designs, consisting of two, 4‐week periods. In Experiment 1, the total dry‐matter intake followed a significant quadratic relationship (P < 0·05), increasing with low levels of straw inclusion and decreasing at higher levels of inclusion. With increasing levels of straw inclusion, there was a linear decline in milk yield (P < 0·001) and milk protein concentration (P < 0·05), but milk fat concentration was unaffected (P > 0·05). In Experiment 2, the effect of straw inclusion on total dry‐matter intake was quadratic (P < 0·001), with intakes being maximum at the 1·0‐kg level of straw inclusion. Milk yield exhibited a linear decrease (P < 0·001) with increasing level of straw inclusion. Milk fat concentration was lowest at the 1·0 kg rate of straw inclusion (P < 0·05), but milk protein concentration was unaffected by straw inclusion. There were no significant interactions between basal forage type and level of straw inclusion for any of the variables examined (P > 0·05). Despite small increases in total dry‐matter intake at a low level of straw inclusion, there was no evidence that straw inclusion improved either nutrient utilization or animal performance. The reduction in milk yield observed with straw inclusion reflects, to a large extent, a reduction in metabolizable energy intake.     

Yield and nitrogen concentration of above- and below-ground biomasses of red clover cultivars in pure stands and in mixtures with three grass species in northern Europe

Three red clover (Trifolium pratense) varieties differing in productivity and winter hardiness, Jokioinen, Betty and Ilte, were sown in a 2‐year (2003–2004) pot experiment in pure stands and mixtures with the grasses, timothy (Phleum pratense), tall fescue (Festuca arundinacea) and meadow fescue (Festuca pratensis). Grass growth dominated until fertilizer‐N, applied when the stands were sown, was depleted. Timothy was the least competitive of the grass species. Red clover variety Ilte produced the highest dry‐matter (DM) yields. Variety Betty yielded less, but allocated as much biomass to the root and stubble (soil‐bound) fraction as variety Ilte. Variety Jokioinen allocated least to the soil‐bound fraction. While the root structure and the starch concentration of the crown‐root area were similar in all varieties, the high ratio of soil‐bound: harvested fractions could be a key to the higher winter survival and higher DM yields of Betty under field conditions. At the end of the experiment, 3–5 g N pot^?1 (49–81 g m^?2) had been harvested and 0·7–1·5 g N pot^?1 (11–24 g m^?2) was left in the soil‐bound fraction, amounts depending on the red clover variety and grass mixture, with pure clover stands containing the highest N amounts. Because of the high N concentrations in the biomass of red clover, the proportion of red clover and conditions prevailing during canopy and root death in mixed stands are crucial for N mineralization and incorporation into new growth.