AXENIC CULTURE OF FREE-LIVING CONCHOCELIS OF PORPHYRA YEZOENSIS AND PORPHYRA HAITANENSIS

ＩＮＴＲＯＤＵＣＴＩＯＮＡｘｅｎｉｃｃｕｌｔｕｒｅｏｆａｌｇａｅｉｓｒｅｑｕｉｒｅｄｆｏｒｍａｎｙｅｘｐｅｒｉｍｅｎｔａｌｓｔｕｄｉｅｓａｎｄｃｅｌｌｓｕｓｐｅｎｓｉｏｎｃｕｌｔｕｒｅｓ,ａｎｄｉｓｅｓｓｅｎｔｉａｌｆｏｒｇａｉｎｉｎｇｕｎｄｅｒｓｔａｎｄｉｎｇｏｆｔｈｅｒｅｌａｔｉｏｎｓｈｉｐｂｅｔｗｅｅｎｔｈｅａｌｇａｅａｎｄｔｈｅｉｒｅｐｉｐｈｙｔｉｃｆｌｏｒａａｎｄｍｉｃｒｏｏｒｇａｎｉｓｍｓ,ａｎｄｆｏｒｓｔｕｄｙｉｎｇｔｈｅｇｒｏｗｔｈａｎｄｄｅｖｅｌｏｐｍｅｎｔｏｆａｌｇａｅ (Ｔａｔ…     

DEVELOPMENT OF SUSPENDED CONCHOCELIS OF PORPHYRA HAITANENSIS (BANGIALES, RHODOPHYTA)

INTRODUCTIONAferthelifehistoryofPorphrmwasdescrihed(Drew,l949;KUIDgi,1953;TsengandChang,l954),researchwasfocusedonthegrowthanddevelopmentofPorphpecon-chocelis(TsengandChang,1955;IwasakiandMatsudaira,l963;Waalandetal-,1987).PorphrmhaitanensisisaChinesespeciesveryimPOrtanforcultivationforcom-mercialproductioninsouthemChina.ConchocelisinshellsisthemainsoureeOfcon-chosPoresusedtoseedtheculturenets.However,ourknOWedgeofPoIPllyTahaitanensisisstilllithetedandtheinformationconcerningsee…     

THE ULTRASTRUCTURE OF SEPARATED AND CULTURED CELL OF PORPHYRA YEZOENSIS

There are many reports that cells (protoplasts) separated from the thallus of Porphyra by enzyme can develop to normal leafy thalli in the same way as monopores. But there are few investigations on the subcellular structure of the isolated vegetative cell for ccmparison with the subcellular structure of monospores. To clarify whether the separated and cultured cells undergo the same or similar ultrastructure changes during culture and germination as monospores undergo in their formation and germination, we observed their ultrastructure, ccxnpared them with those of the monospore and found that the ultrastructure of separated and cultured cells did not have the characteristic feature as that of monospore formarion, such as production of small and large fibrous vesicles, but was acccxnpanied by vacuolation andstarch mobilization like that in monospore germination. The paper also discusses the relations between monospores and separated and cultured cells.     

Effect of cytokinins and auxins on the growth of free-living conchocelis ofPorphyra yezoensis

IAA 3-Indolylacetic acid, NAA a-Naphthylacetic acid and cytokinins in PESI culture medium were used in a study on the effects of plant hormones on the growth of free-living conchocelis ofPorphyra yezoensis which showed that its growth in medium with cytokinins, IAA and NAA was more rapid than that in medium with non—phytohormones; that the optimal concentrations for promoting growth were 10 μg/L for IAA and ZA (Zeatin), and 0.1 μg/L for BA 6-Benzyl amino purine and KIN 6-Furfurylamino- purine. Mix use of NAA, IAA and cytokinins, NAA/ZA 1–1000/1 μg/L, NAA/BA 10/1-1000 μg/L, NAA/KIN 1/1-1000 μg/L promoted growth. IAA/ZA 0.1-1/0.1-1 μg/L; IAA/BA 0.1-1/0.1-10 μg/L IAA/KIN 1/0.1-1000 μg/L also promoted growth. Contribution No. 2335 from the Institute of Oceanology, Chinese Academy of Sciences. Contribution No. 94 from the Experimental Marine Biology Lab Qingdao, China. The research was supported by a grant from the Chinese Academy of Sciences.     

越南紫菜果孢子放散规律及壳斑藻的培育初步研究

报导了越南紫菜果孢子的放散规律及其壳斑藻的培育情况,并对该种紫菜的人工移植进行了初步探讨。     

COMPARATIVE STUDIES ON STRUCTURAL FEATURE OF AGAR POLYSACCHARIDES FROM PORPHYRA HAITANENSIS GROWN IN SOUTH AND NORTH CHINA

The structural feature of agar polysaccharides from Porphyra haitanensis grown in south China and transplanted to the north was investigated by fractioriation on DEAE - Sephadex A 50, chemical analysis, and infrared and 13C - NMR spectroscopy.The agars composed mainly of Charged molecules were eluted from DEAE - Sephadex A 50 with 1.0 mol/L- NaCl solution from the southern P. haitanensis and with 0.5 mol/L NaCl from the northern one. The 13C-NMR spectra showed that agarobiose [(1 -3)- B D - galactopyranosyl - (1 -4)-3,6-anhydro-a-L-galactopyranose] and the biological precursor of agarobiose [(1-3} B-D-galactopyra nosyl- (1-4)- 6- sulfate-a- L-galactopyranose ] were the major disaccharide repeating units in the charged fractions. The content of the biological precur sor in the agar polysaccharides from southern P. haitanensis was higher than that in the northern one, the content of the biological precursor extracted from cold water was higher than that from hot water, and the content of 6- OMe-D-galactos     

SUBUNIT COMPOSITION AND CHROMOPHORE CONTENT OF R-PHYCOERYTHRIN FROM PORPHYRA HAITANENSIS

R-phycoerythrin from Porphyra haitanensis exists in two aggregaion states with different molecularweights. A more highly aggregated form, RPE I,Was chromatographed on Bio-Rex 70 column with ureasolution (pH 3.0) as eluent, and the molecular weights of the 3 subunits (α,β,γ) obtained were determinedon SDS-PAGE at 18000, 19200 and 30000, respatively. α subunit carried two phycoerythrobilin (PEB),β subunit, three PEB and one phycourobilin (PUB),γ subunit, one PEB and three PUB chromophores.The molar ratio of α,β, and γ subunits of RPE I wn 6:6:1, and their subunit composition was con-fired to be (αβ)_6γon account of the molecular weight of RPE I, 232000.A lower aggregated form, RPE Ⅱ, contained α and β subunits similar to those of RPE I, but itssubunit composition was the (αβ) monomer of RPE.     

EFFECT OF GERMANIUM DIOXIDE ON GROWTH OF SPIRULINA PLATENSIS

This study on the effect of different concentrations of germanium dioxide (GeO_2) on the specificgrowth rate (SGR), pigment contents, protein content and amino acid composition of Spirulina platensisshowed that Ge was not the essential element of this alga; that GeO_2 could speed up growth and raiseprotein content of S. platensis, and could possibly influence the photosynthesis system. The concentrationrange of GeO_2 beneficial to growth of S. platensis is from 5-100 mg/l. GeO_2 is proposed to be util-ized to remove contamination by Chlorella spp. usually occurring in the cultivation of Spirulina.     

USE OF RAPD FOR DETECTING AND IDENTIFYING PORPHYRA (BANGIALES, RHODOPHYTA)

imDUCnONManymolectilarbiologybasedDNAtecboqUesPbodepowrfulmeansforstUdvingofgeneticvariationandphylDgenehcrelationships.AmoleculartecboqUebasedonthePOl}-menachainreaction(PCR)isaneffeChvetoolforraPididentificationofgeneticTnarersknownasRandomAInPlifiedPolymorphicDNA(m)(Wlliamsetal.,l99o;Welsh&McClelland,l99o).Sucheders,derivedfromPrindngsitesrandoInldistributedthIDughoutthegenome,areinheritedinaMendelianPatem.ThesePOlyTnorphismsallowtheanalsisofcomplexgenomeswithoutPriorknoWl…     

浅论经济增长与物价水平的关系

从交易方程出发，初步分析了经济增长与物价水平的关系，得到了一些有益的结果。     

THE EFFECTS OF RECOMBINANT EEL GROWTH HORMONE,METHYLTESTOSTERONE AND L-THYROXINE ON THE GROWTH OF REDSEABREAM,PAGROSOMUSMAJOR

The effects of recombinant eel growth hormone (reGH).methyltestosterone (MT)and L-thyroxine (T4)on the growth of red sea bream. Pagrosomus major.were investigated.Administration of reGH to fry by immersion at 2 mg 1 for 2 hevery 5 dsys resulted in significant increase in both weight and length.but the condition factor (CF) diminished relative to that of similarly treated controls over the 37day treatment period.Immersion in 0.1 mg:1 T4 also resulted in significant increase in both weight and length and higher survival rate of test fry compared to the controls. Immersion in MT had less effect on growth and high-dose resulted in high mortality.In the second study.injection of 2 μg reGH(gwk)caused a significant increase in the specific growth rate (SGR) of test red sea bream fingerlings relative to that of the controls during the 4-week treatment period and maintained the increasing trend over the post-treatment period (weeks 4-6).Injection of MT at a dosage of 1μg (gwk) resulted in a significant in-creas     

蜕壳促长素对斑节对虾、刀额新对虾蜕壳和生长的作用

在对虾人工配合饲料中添加蜕（脱）壳促长素，喂养斑节对虾（ＰｅｎａｅｕｓｍｏｎｏｄｏｎＦａｂｒｉｃｉｕｓ）和刀额新对虾（ＭｅｔａｐｅｎａｅｕｓｅｎｓｉｓＤｅＨａａｎ），观察其促进蜕壳、生长的作用。试验证明：饲料中添加不同浓度（０．５‰。、１．０‰、１．５‰）的蜕壳促长素，均不同程度地促进对虾的蜕壳和生长。添加浓度为１．５％。效果最为明显。值得在生产中应用。     

EFFECT OF NITROGEN AND PHOSPHORUS ON THE GROWTH OF A RED TIDE DINOFLAGELLATE SCRIPPSIELLA TROCHOIDEA (STEIN) LOEBLICH III

INTRODUCTIONRedtidecanadverselyaffectcoastalfisheriesbycausingthedeathofculturedfishandshrimporproducingtoxinsthatcanbeaccumulatedthroughfoodchains (Anderson ,1 989) .Phytoplanktonisoftenthemaincomponentofmanyredtides.AlongthecoastofJiaozhouBay ,P .R .China,theredtideismainlyformedbyadinoflagellate ,Scrippdiellatrochoidea ,duringsummer (JulytoSep tember)andthecelldensitycanreach 9.98× 1 0 7cells ml (Zou ,unpublisheddata) .Manyresearcherssuggestedthatredtideofphytoplanktonmightresultf…     

PROMOTIVE EFFECT OF Se ON THE GROWTH AND ANTIOXIDATION OF A BLUE-GREEN ALGA SPIRULINA MAXIMA

ItwasreportedthatthegrOwthofthedrinediatomffelassforiraPsethenana(Hust.)HasleetHeiIndaldependedonSe(Priceetal.,1987).ThisreqUirementissPecificforSe;nootherelementscansubstituteforit,becauseSecaninduceglutathioneperoxidase(GSH-PoD)activityinthisdiatom(PriceandHarrison,l988),andinlow-CO2-grooCham~nasreinhandiiDangerad(Yokotaetal.,l988).ThisenZyInac-tivitywdetectedinextractsOfablue-greenalgaGleeoCaPasp.LB795(TotaandGallon,l979),andalsoincultUredhigherplancells(Drotaretal.,1985),butw…     

EFFECT OF RATION, TEMPERATURE AND BODY WEIGHT ON GROWTH OF BLACK PORGY,SPARUS MACROCEPHALUS (B.)

ChntnbutionNo.3127fromtheInstituteofOceanology,ChinereAcademyofSciences.Project39370542supportedbyNSFCandstudyalsosupportedbyNationalKeyLaboratoryintheInstituteofHydrobiology.ChineseAcademyofSciences.lNTRODUCTIONltisknownthatgroWthrateoffishcanvary'inresponsetotemperatureandfoodavailability-andperhapslessobviously,tolight,oxygenandothervariables.ThispropertyofvariablegroWthinfishcanbereferredtoasplasticity(Weatheriey,l990).TheeffeCtSofenvironmenta1thCtorsongroWthcanbepredictalfor…     

STUDIES ON INTERCROPPING OF PORPHYRA HAITANENSIS T.J.CHANG ET B.F.ZHENG WITH BAY SCALLOP ARGOPECTEN IRRADIANS LAMARCK

Results of the experiment of northward transplantation of Porphyra haitanensis showed thatintercrop of P. haitanensis with Bay Scallop is feasible because the growth duration of both of them is about the same in August - December.Full use of cultivating area, lowering its cost and increasing its product per unit area as well as improving the ecological envronment are realized during intercrop.     

白鲢仔稚鱼食性与生长的初步研究

池塘培育的鲢仔稚鱼（６．８—３２．２ｍｍ）食性可分为浮游动物和浮游植物为主的二个阶段。食性的转化取决于消化系统各器官的发育分化。全长１５ｍｍ左右为食性转化阶段。全长２７．９～３２．２ｍｍ，食性接近成鱼。口宽（ＭＷ）、口高（ＭＨ）与全长（Ｌ）的关系分别为：ＭＷ＝－４０１．９９＋９２．６２Ｌ、ＭＨ＝－２２１．６２＋４９．６６Ｌ；肠长／全长（Ｋ）与全长（Ｌ）的关系为：Ｋ＝－０．１２４８＋０．０７１３Ｌ；第Ⅰ列外侧鳃耙数（ＧＮ）与全长（Ｌ）的关系为：ＧＮ＝－６７．４４＋８．９３Ｌ；全长（Ｌ）体重（Ｗ）和时间（ｔ）的关系分别为：Ｌ＝４．５３７＋１．４１７ｔ、Ｗ＝２．７８５ｅ０．２８７３ｔ。以上回归方程经统计检验均为高度显著相关。     

EFFECT OF ARTIFICIAL REGULATIONS OF ARTEMIA n-3 HUFA CONTENT ON GROWTH AND SURVIVAL OF BLACK SEABREAM (SPARUS MACROCEPHLUS) LARVAE

ImODUCTIONStudiesonthenutritionmpuirementSoffishlarvaehavernadesomeprognaabroad,butwearenotawareofreportSonsuchstudiesinChinanow.ltwasdeter-Annedanhythatn-3HU-FA,espedally22f6(n-3)roHA)and2O5(n-3)(EPA)ises-sentialformarinefishlarvaegroWth.ThehighcontentofDHAinmarinefisheggSandthel0wcontent0fDHAinArtrmiashowthatArthetaasliwfndcandsaoothen-3HUFAreq~dmendfishlacae.bosdenistSdriitaln-3HUFAenriedArbotabyboingthemn-3HUFAcontainingbogtanulataldietS,etC.Watanabe,T.(l983)enri~livefe…     

试论板缘仰冲作用及其对板内构造的影响

板块学说发展史上存在着造山作用的仰冲和俯冲两种成因观点。俯冲说难以解释中脊俯冲、板缘构造、板内变形及海沟构造。仰冲说认为板块会聚边缘处在两个相向运动着的对流系交会带。根据板缘构造平、剖面特点,结合威尔逊旋回、板块运动史、地壳结构等分析,大陆仰冲板块是在克服属弱对流系一方的洋板块的抵抗,迫使贝尼奥夫带不断向洋退却过程中向前推进.而造成各种板缘和板内构造的变形机制的。据此,提出一个断续仰冲模式以代替传统的连续俯冲模式。用仰冲观点分析了形成板内变形的内、外条件和常见的变形型式。还试用板内仰冲作用来解释华南加里东期构造特点与钦州地槽之谜。     

EFFECT OF ANTHROPOGENIC CO_2 ON THE pH AND THE SATURATION STATES OF CALCITE AND ARAGONITE OF SEAWATER

unODUcrIONIntereStinthecarboncycehasinamsedbouseoftheobservedincreaseinatmosphericCO,leveIsduetothebumingoffossilfuelandthedearingofforestsre-sultinginlargedhaticandbiogeochdrica1consequenas(ChenandDrake,l986).Experi-mentalstudisofp1antShaveshownresponsesoftypically2O%-4O%highergroWthunderdoubledCo,conditions.Ontheotherhand,theeffedofelevatalCO=conditionsinincreasinggroWth,knownastheCO,fertilimtioneffed,isnotbe1ievedtobetwortantintheoasns(Schimeletal.,1995).Althoughithasbeensuggestedt…