兰州拉面专用粉对春小麦品质的要求——Ⅱ小麦籽粒面筋蛋白组成含量与品质的关系

共选用30份春小麦材料,参照Singh等[1]和Verbruggen等[2]方法,提取出不同材料的面筋蛋白、高分子量麦谷蛋白亚基(HMW-GS)和低分子量麦谷蛋白亚基(LMW-GS)。通过测定各蛋白组分的含量及比例,同时结合不同材料的理化品质、面团流变学特性以及拉面食用品质进行多元相关分析,结果表明:麦谷蛋白含量与小麦籽粒蛋白含量、湿面筋含量、面筋指数、干面筋含量呈显著正相关;与面团弹性、延伸性以及面团筋力也呈显著正相关;与拉面食用韧性、粘性以及总评分呈显著正相关。HMW-GS含量与小麦湿面筋和干面筋含量呈显著正相关;与面筋指数呈显著负相关。在面团品质方面,HMW-GS含量与面团韧性呈显著正相关,而与LMW-GS与面团延伸性和面团筋力为显著正相关。HMW-GS含量与拉面最终评分呈显著负相关,相反LMW-GS与拉面最终评分呈显著正相关。     

Rheological properties and baking quality of commercial durum wheat meals used to make flat crispy bread

Four commercial semolinas and four low-grade semolinas (LGS) samples were used in this study to make flat crispy bread (Carasau). The meals, doughs, and bread were compared with respect to a set of physical, chemical, and textural characteristics, and the bread evaluated by a trained sensory panel. LGSs had a higher extraction rate (higher ash content), finer granularity, and a higher damaged starch content than semolinas. The study revealed the key role of dry gluten content in determining hydration capacity of the dough, whereas damaged starch affected water absorption only in semolina samples. Gluten index and the ratio of gluten tenacity to extensibility (alveograph P/L) were significantly correlated with the weight of the dough discs following sheeting and molding. The panelists gave the highest quality score to the bread with the highest values of crackling and hardness. Textural and sensory attributes were both correlated with one another, and with gluten strength and tenacity, but not with the gluten content of the meal, which only affected final moisture content of the bread. Meal samples with a more tenacious and elastic gluten were more suited to produce flat crispy breads. The yellowness of durum wheat bread could not be predicted by measuring the color of the meal with a reflectance method.     

Evaluation of standard physico-chemical and rheological parameters in predicting bread-making quality of durum wheat (Triticum turgidum L. ssp. durum [Desf.] Husn.)

The efficacy of empirical rheological methodology for modern Triticum aestivum L. in predicting bread-making quality in Triticum turgidum L. ssp. durum is warranting of attention. The present study was aimed at providing a comparative evaluation of an array of standard rheological tests in predicting bread quality in three modern cultivars (Core, Sant’Agata and Simeto) and three durum wheat landraces (Ruscìa, Timilìa and Tumminia SG3). Gluten strength, alveograph dough strength and tenacity/extensibility ratio, farinograph stability and mixograph peak dough height and overall score, were significantly higher in the modern cultivars, and distinctive in accounting for the variance between modern and old genotypes, using principal component analysis. Quality bread-making parameters, including bread volume, height and specific volume, were significantly higher in the durum wheat landraces. Standard rheological parameters were ineffective in predicting bread quality, necessitating the requisite in redefining rheological parameters to meet the increasing interest in heritage durum wheat genotypes for bread-making.     

Hearth bread baking quality of durum wheat varying in protein composition and physical dough properties

Thirty durum wheat genotypes from ten countries of origin were grown in field plots for two consecutive years. Three of the genotypes were γ‐gliadin 42 types and the remainder were γ‐gliadin 45 types. Among the γ‐gliadin 45 types, six high‐molecular‐weight glutenin subunit (HMW‐GS) patterns were identified: 6 + 8, 7 + 8, 7 + 16, 14 + 15, 20 and 2*, 20. All the γ‐gliadin 42 genotypes contained low amounts of unextractable polymeric protein (UPP) and exhibited low gluten index values and weak gluten properties. The γ‐gliadin 45 genotypes exhibited a wide range of UPP, gluten index and dough strength. HMW‐GS 20 genotypes were generally weak, whereas HMW‐GS 6 + 8 and 7 + 8 genotypes were generally strong. When baked by a lean formulation, long‐fermentation straight‐dough hearth bread process, the durum wheat genotypes exhibited a wide range of baking quality. Loaf volume and bread attributes were strongly correlated with UPP and gluten index. Some of the genotypes exhibited bread attributes and loaf volume equal or slightly superior to those of a high‐quality bread wheat flour. However, even the strongest durum wheat genotypes exhibited inferior fermentation tolerance to the bread wheat flour, as seen by a requirement for lower baking absorption during dough handling and more fragile dough properties when entering the oven. Among the HMW‐GS groups, HMW‐GS 7 + 8 and 6 + 8 exhibited the best and HMW‐GS 20 the poorest baking quality. Farinograph, alveograph and small‐scale extensigraph properties demonstrated that a combination of dough elasticity and extensibility was needed for superior durum wheat baking performance. Copyright © 2007 Society of Chemical Industry     

Relationship between gluten strength and pasta firmness in Indian durum wheats

Selected Indian durum wheats including five newly released varieties and seven landraces were studied for their grain quality, gluten strength (sodium dodecyl sulphate‐sedimentation and mixograph) and pasta making properties. Landraces were found to have good grain size, protein content, and gluten strength but they had lower test weight and semolina yield than released varieties. As protein composition affects gluten strength, landraces having combination of low molecular weight (LMW‐GS) with 5, 12, 15 and 19 linked to Gli‐B1 43.5 and high molecular weight (HMW‐GS) 2*, 14 + 15 provide a dough strength comparable with the 7 + 8 and LMW‐GS 2, 4, 6, 12, 15 and 19 (caa) allelic pattern, typically associated with good gluten strength. Landraces have rare combinations of glutenins and gliadins, which are not seen in commercial Indian durums, and in some cases, these rare alleles seem to favour good gluten strength and pasta firmness. Introduction of these alleles through breeding should improve the gluten strength and pasta making properties of Indian durum cultivars.     

The effect of the combination of reducing and oxidising agents on the viscoelastic properties of dough and sensory characteristics of buns

BACKGROUND: The effects of mixtures of reducing and oxidising agents on the rheological characteristics and quality of wheat flour dough and the sensory characteristics of buns were studied. RESULTS: In chemical analyses, no differences between control dough and doughs with individual mixtures of additives were found. Rheological measurements showed that increasing addition of L ‐ascorbic acid + L ‐tryptophan mixture to the dough increased its tenacity and decreased its extensibility. Increasing addition of L ‐ascorbic acid + L ‐threonine mixture also led to an increase in dough tenacity but to a slight increase in dough extensibility. Increasing addition of L ‐ascorbic acid + inactivated dry yeast mixture to the dough resulted in a decrease in tenacity and an increase in extensibility. Two other additive mixtures, L ‐ascorbic acid + L ‐cysteine hydrochloride monohydrate and L ‐cysteine p.a. + inactivated dry yeast, reduced both the tenacity and extensibility of the dough. In sensory analyses, differences in dryness, pliability, sensation when swallowing, quality and texture of buns with additive mixtures were found in comparison with control buns, but gumminess did not change significantly with increasing amount of additive mixtures. CONCLUSION: The additive mixtures tested had a positive influence on bakery products. Copyright © 2010 Society of Chemical Industry     

糖对面团工艺性能的影响

以不同的糖分别加入到高筋粉和低筋粉中,研究糖对面团的工艺性能的影响。结果表明：面国才的面筋含量随着用糖量的增加而降低,且双糖的影响大单糖。还原糖和非还原糖对面团延性的影响也不同。当还原糖用量在20％以内时,面团的延伸随用糖量的增加而增大,当用糖量大于20％后,面筋易断裂,延伸性减小;非还原糖则无此现象。     

氧化淀粉对小麦面团品质变化影响研究

通过对面筋强度、巯基和二硫键含量测定、面筋蛋白电泳观察分析及面团流变学特性变化,研究氧化淀粉对小麦面团品质特性影响。结果表明,氧化淀粉能增加面筋乳酸溶涨值和透光率,提高面筋强度;对面筋蛋白具有氧化作用,增加大分子麦谷蛋白亚基含量,对面团粉质特性影响不显著;但能改善面团拉伸性能,其中以羧基含量为0.27%至0.40%氧化度氧化淀粉对面团品质变化影响最为显著。     

变性淀粉对面团流变学特性和面包品质的影响

探讨不同添加量的马铃薯变性淀粉和木薯变性淀粉对面团流变学特性和面包品质的影响。结果表明:两种变性淀粉均可改善面团的流变学特性,增大面团的韧性、减小面团的延伸性,改善面团的筋力;与对照相比,马铃薯变性淀粉能有效改善面包的焙烤品质,明显增加面包的比容,有效改善面包的硬度和弹性,木薯变性淀粉对面包品质的影响不明显;马铃薯变性淀粉的最适添加量为0.8%～1.2%面粉。     

The influence of 1B/1R chromosome translocation on gluten protein composition and technological properties of bread wheat

The Austrian bread wheat Amadeus without and with 1BL/1RS translocation and three further translocation genotypes with known HMW subunit compositions were grown under the same environmental conditions. Their flours were characterised by the determination of crude protein content and, partly, by the determination of glutathione and cysteine. Furthermore, the qualitative and quantitative composition of gluten protein types was analysed by a combined extraction and reversed phase HPLC procedure. Dough development time, maximum resistance and extensibility of dough and gluten, and bread volume were determined by means of microscale methods. Protein, glutathione and cysteine contents of flours were only slightly influenced by translocation. The HPLC patterns of gliadins and glutenin subunits showed that translocation caused characteristic changes concerning ω‐gliadins, γ‐gliadins and LMW subunits of glutenin. The amount of ω 1,2‐gliadins was significantly increased and that of LMW subunits decreased. The effect of translocation on the rheological properties of dough and gluten was characterised by a strongly reduced dough development time, reduced maximum resistance and increased extensibility. Bread volume was decreased by about 10%. The amount of glutenin subunits was correlated with dough development time, resistance of dough and gluten, and bread volume to a higher extent (r = 0.79–0.91) than the amount of gliadins (r = 0.52–0.80). Correlation coefficients for LMW subunits were higher (r = 0.82–0.88) than those for HMW subunits (r = 0.35–0.61) when all five wheats were included. Instead, when only translocation lines were considered, HMW subunits (r = 0.89–0.98) were more important than LMW subunits (r = 0.64–0.86). Altogether, the results demonstrate that translocation causes important quantitative as well as qualitative changes in gluten protein composition which can be efficiently determined by reversed phase HPLC. © 2000 Society of Chemical Industry     

Effect of soybean addition on the rheological properties and breadmaking quality of wheat flour

The effects of enzyme‐active full‐fat (EAFFSF), heat‐treated full‐fat (HTFFSF) and enzyme‐active defatted (EADSF) soy flours and commercial soy protein isolates (SPIs) on mixing properties and extensibility of dough, gluten formation, gas production and retention properties of dough and bread quality were studied. The soy products utilised in this study presented values of urease activity, nitrogen solubility index (NSI) and enthalpies of protein denaturation according to their previous heat processing. Soy products interfered in gluten formation, weakened dough strength and decreased dough gas retention capacity. Bread quality was negatively affected by soy product addition. The negative effects were exacerbated by an increase in soy/wheat ratio. Soybean protein state was identified as an important factor in determining dough and bread properties. Copyright © 2005 Society of Chemical Industry     

Preparation and evaluation of chili powder-enriched layered noodles

Chili powder-enriched dough was sandwiched between two dough layers prepared from (a) wheat flour, (b) wheat flour plus resistant starch flour and (c) wheat flour plus soy protein isolate (SPI) and microbial transglutaminase (MTGase), and these were used for preparing wheat flour control (LN-C), wheat flour-resistant starch (LN-F) and wheat flour-SPI-MTGase (LN-S) noodles, respectively. All cooked noodles were assessed for physical properties, starch digestibility and capsaicin-retaining abilities. Compared to other noodles, the LN-S noodles exhibited the highest tensile strength and elasticity, and the highest capsaicin retaining ability at pH 7.4. However, at pH 1.2 the capsaicin-retaining ability of all noodles was similar. The predicted glycemic indices (pGIs) of LN-F and LN-S noodles were similar (P > 0.05), and were significantly (P < 0.05) lower than that of LN-C noodles. In conclusion, the resistant starch flour helped to lower pGI of the layered noodles, but it was not effective at retaining capsaicin. The occurrence of additional cross-linking between protein matrices of LN-S noodles could be a factor that had impaired structural integrity and retarded the diffusion of capsaicin from the capsaicin-enriched dough.     

The effects of different materials as dough improvers for organic whole wheat bread

In this research, the effects of different materials such as defatted Cephalaria syriaca flour (0.5%), rosehip (2.5%), vital gluten (2.5%) and malt flour (2%), and their combinations on the quality of organic whole wheat flour were investigated. The highest maximum resistance value was obtained in the treatment containing 0.5% cephalaria and 2.5% rosehip. The addition of malt flour and vital gluten significantly increased the extensibility value. Although rosehip, cephalaria and vital gluten generally increased the dough energy, malt flour decreased the dough energy when compared to the control. The combination of 0.5% cephalaria and 2.5% rosehip significantly decreased the adhesion and stringiness of dough. Data showed that dough rheological characteristics of organic whole wheat flour could be improved with the addition of different materials such as malt flour, cephalaria, rosehip and vital gluten.     

Rheological properties of gluten derived from wheat cultivars with identical HMW glutenin subunit composition†

Ten spring wheat cultivars possessing identical HMW glutenin subunits (2*, 7+8, 5+10) were evaluated for gluten and protein content. Gluten content was related to flour protein content (r=0·98). Addition of freeze-dried gluten to the base flour (cv Alpha) to a constant protein level of 12% generally increased dough strength. However, the magnitude of variation in mixing patterns depended more on the type of the supplemental gluten. Fortification of the base flour with the freeze-dried gluten from the cv Glenlea produced mixographs with the longest mixing development time (MDT), and highest band width energy (BWE) and energy to peak (ETP), suggesting that the source of gluten had a strong effect on dough rheology. The viscoelastic properties of undiluted wet gluten varied between cultivars during mixing reflecting differences in gluten quality. Freshly prepared wet gluten of Glenlea showed extended mixing tolerance as compared to Norseman or Alpha gluten. The wet gluten from cv Glenlea was less extensible with high maximum resistance to extension and had a larger area under the extensigraph curve than gluten obtained from cv Norseman. Gluten prepared from the cultivars Glenlea, Bluesky and Wildcat were less soluble in aqueous propanol and produced more froth when the dough was washed with deionised water. The froth proteins, separated by SDS-PAGE, predominantly contained strongly stained bands in the region corresponding to molecular weight <50 kDa. The rapid tests such as froth formation and alcohol solubility used in this study to discriminate various glutens were highly correlated with the mixograph parameters. These methods can be of practical value in evaluating gluten quality. © 1998 Society of Chemical Industry.     

Investigating the positive effects of wrap-around resting on the qualities of semi-dried noodles through the quantitative analysis of gluten network

In this study, the dough sheet wrap-around was employed to assist the resting process of the semi-dried noodles comparatively with dough crumbs resting and common dough sheet resting. The gluten network quantitative analysis was carried out to investigate the positive impacts of dough sheet wrap-around resting in semi-dried noodles production. The results showed that the dough sheet wrap-around resting improved the color, surface smoothness, cooking qualities, and eating qualities of semi-dried noodles. Dough sheet wrap-around resting for 30 min significantly (p < 0.05) increased the surface smoothness and chewiness by 47.08% and 44.35%, respectively. Furthermore, increased extensibility in the transverse direction of dough sheets generated superior processing properties. The average protein length and width of dough sheets experienced a considerable (p < 0.05) reduction. In contrast, the branching rate was markedly (p < 0.05) augmented, which meant the distribution of gluten network was more uniform and denser. The total protein length and the number of protein network lines both significantly (p < 0.05) increased. The number of transverse protein network lines increased by 28.70%, which was much higher than that (5.77%) of the longitudinal direction. Conclusively, at the optimal dough sheet wrap-around time of 30 min, the higher-quality semi-dried noodles were produced by enhancing the gluten network.     

蒙古栎橡子-小麦混粉的流变学特性

将蒙古栎橡子粉与小麦粉按不同的比例混合,利用布拉本德粉质仪与拉伸仪测定蒙古栎橡子-小麦混粉面团的流变学特性,并对混粉中的理化指标与混粉面团流变学参数之间进行相关性分析,探讨蒙古栎橡子粉对小麦粉品质的影响。结果表明：随着橡子粉含量增加,混粉的吸水率呈先减小后增大的趋势,面团的形成时间与稳定时间缩短,弱化度提高;所有样品的拉伸阻力与拉伸比例随醒发时间的增长而增大,而混粉面团的延伸度与能量均呈下降趋势;在相同的醒发时间下,拉伸参数随橡子粉添加比例的增加呈先增大后减小的趋势。与混粉面团流变学特性呈高度正相关的理化指标是混粉面团湿面筋及蛋白质的含量。提示不同比例的蒙古栎橡子粉可以显著影响面团的流变学特性。     

Mechanistic study of the impact of germinated brown rice flour on gluten network formation,dough properties and bread quality

The objective of the study was to investigate the impact of germinated brown rice flour (GBRF) on the mechanism of high gluten wheat flour (HGWF) network formation and dough properties. Therefore, dough properties, microstructures, stability mechanism and bread quality were evaluated. Results showed that HGWF+10%GBRF exhibited a higher dough stability time (DST), pasting temperature, storage modulus (G′) and loss modulus (G′′), as well as a lower compliance value. The microstructure of dough showed that GBRF interfered with the self-organization of gluten protein molecules and affected the formation of gluten network structure. The disulfide bonds and β-sheet structure were proved to play an important role in facilitating the formation of a stable three-dimensional network structure, which revealed the regulatory mechanism of GBRF in maintaining the dough stability and strength. Furthermore, the dough mixing properties and texture parameters (i.e., hardness and fracturability) of breads were significantly correlated. Overall, GBRF can be used as a potential ingredient for whole grain products and its realistic role in bread-making has been demonstrated.     

采用Mixolab 和Rheometer 研究含外源蛋白燕麦面团的热机械学和动态流变学特性

通过酶流变分析仪(Mixolab)和动态流变仪(Pheometer)研究3种不同外源蛋白对燕麦面团体系热机械学和流变学特性的影响。Mixolab 分析结果表明：大豆蛋白和面筋蛋白都使燕麦面团的吸水率增大,蛋清蛋白使吸水率显著下降。3 种蛋白的添加都可显著提高面团形成时间,其中面筋蛋白影响最大,大豆蛋白最小。面筋蛋白和蛋清蛋白都使燕麦面团的稳定时间显著增加,其中含10% 面筋蛋白和15% 蛋清蛋白的燕麦面团具有最好的稳定性。含蛋清蛋白的燕麦面团在淀粉糊化时获得最大的峰值扭矩和回值,且随着其添加量增加而有所增大,大豆蛋白则相反,面筋蛋白对相关参数影响较小。动态流变学实验显示：含面筋蛋白和大豆蛋白的燕麦面团具有较大的弹性模量(G')和黏性模量(G"),且随着添加量的增大而增加,含蛋清蛋白的面团则相反。采用DSC 进一步分析面团的热力学特性显示：蛋清蛋白和大豆蛋白可显著提高燕麦面团的峰值温度和终止温度,并在一定程度上提高面团的热焓值;面筋蛋白则作用不明显。     

Enzymatic cross-linking to improve the handling properties of dough prepared within a normal and reduced NaCl environment

This research examines the use of three enzymes [glucose oxidase (GOX), hexose oxidase (HOX), and xylanase (XYL)] and their combinations [GOX–XYL and HOX–XYL] on the dough handling properties of CDC Plentiful and Stettler wheat cultivars prepared at reduced (1.0% wt. by flour) and normal (2.0% wt. by flour) NaCl levels. Properties investigated include dough rheology, stickiness, and ratio of resistance to extension and extensibility. The inclusion of XYL and its combinations with GOX and HOX increased the stickiness, yielded lower dough strength indicated by rheology, and reduced the ratio of resistance to extension and extensibility. The inclusion of oxidative enzymes yielded a stronger dough, where HOX addition to dough had the lowest stickiness values and highest |G*| values, whereas GOX addition led to the highest ratio of resistance to extension—extensibility. NaCl only had minor effects overall on dough strength and stickiness for the cultivars studied. Overall, superior dough handling properties were observed with oxidative enzyme addition (GOX and HOX) suggesting that the increased crosslinking that occurs could aid in improving low sodium bread dough properties.     

Studying the effects of whole-wheat flour on the rheological properties and the quality attributes of whole-wheat saltine cracker using SRC,alveograph, rheometer,and NMR technique

Quality attributes of soft wheat products are affected by physicochemical characteristics and rheological properties of wheat flour. Whole-wheat flour has a significant impact on baking qualities (stack height, stack weight, specific volume, and breaking strength) of whole-wheat saltine crackers due to its high water absorption capacity. SRC profiles, alveograph and rheometer parameters were determined to observe the effect of whole-wheat flour on whole-wheat cracker flour blends. NMR technique was utilized to demonstrate the water migration and competition in whole-wheat dough components. Results of SRC testing revealed that the water absorption of whole-wheat flour blends increased with the addition level of whole-wheat flour. The rheological properties (G′, G″, P, L, W values) were influenced significantly by the presence of whole-wheat flour. Results of NMR indicated that water migrated from gluten network into arabinoxylans matrix in whole-wheat dough system, resulting in inferior saltine cracker-baking qualities of whole-wheat flour, i.e., small breaking strength, stack height and specific volume. The stack height, specific volume, and breaking strength of end products showed significant correlations with the arabinoxylans, dough extensibility, and gluten index of whole-wheat flour.