ANTISPERMATOGENESIS EFFECT OF GTW ON RAT GAMETOGENESIS AND SPERMATOZOA

Forty four adult male rats weighing about 250 g were divided into 3 groups: Group 1 received GTW at a does of 10 mg/kg/d, Group 2, 20mg/kg/d, and Group 3 was givea 1% carboxymethy! cellulose as control. The rats were sacrificed on week     

ANTIFERTILITY EFFECT OF THE MULTIGLYCOSIDES OF TRIPTERYGIUM WILFORDII （GTW） ON MALE RATS AND ITS MECHANISM OF ACTION

It has been reported that GTW caused infertility in male rats and mice, In the present study, GTW was administered orally at a daffy dose of 10 rag/kg, 6 days a week:for 8 weeks to adult male rats. After treatment with the drug, the body weight growth of the     

EFFECT OF MULTIGLYCOSIDES OF TRIPTERYGIUM WILFORDH （GTW） ON RAT TESTIS, HEART, LIVER AND KIDNEY

Adult male Wistar rats were given GTW orally at 50 rag/kg or 20 mg / kg for 4 or 5 weeks. Control animals were given the vehicle only. ARer treatment, testis, heart, liver and kidney were removed and examined. The scminiferous tubules of the treated     

INFLUENCE OF TRIPTERYGIUM WILFORDII ON OVARY AND UTERUS ENDOMETRIUM OF MOUSE

An observation was performed on ovary and uterus endometrium of female mice after administration of a syrup containing root extract of Tripterygium wilfordii Hook.F,( TWH ) for 6 months with light and electron micoscopics. Ovaries were enlarged for 3     

CLINICAL STUDY ON EFFECT OF TRIPTERYGIUM WILFORDII HOOK. F. GLYCOSIDES ON UTERINE LEIOMYOMA

Objective To observe the therapeutic effect and side reactions of Tripterygium wilfordii Hook,f. ( GTW) glycosides on patients with uterine leiomyomas. Methods 65 normally cycling women with symptomatic uterine leiomyomas received 40mg daily dose GTW for 3 to 6 months. Baseline ultrasound tests were obtained to evaluate the sizes of myomas and uterus, then repeated three and six months after treatment. Blood samples were collected to determine the hormonal levels of in the mid-follicular and mid-luteal phases of the menstrual cycles before GTW therapy and at 3~4 months and 5~6 months after treatment. Results Significant decrease in leiomy-oma volume was shown in 39 of 65 (60% ) and 28 of 40 ( 70% ) patients after 3~4 months and 5~6 months of treatment, respectively. The decrease of the volume of leiomyoma was time-dependent as while 27. 84% and 51.60% in 3~4 months and 5 ~ 6months, respectively. 25 of 65 patients had amenorrhea during the course of treatment. Tripterygium wilfordii glycosides treatment     

THE EFFECT OF THE MULTIGLYCOSIDES OF TRIPTERYGIUM WILFORDII ON THE HYPOTHALAMOPITUITARY-TESTIS AXIS OF MALE RATS

The multiglycosides of Tripterygium wilfordii Hook. f. (GTW) is a Chinese herb medicine used traditionally for the treatment of rheumatoid arthritis, chronic nephritis and some skin diseases. It has been recently shown to cause infertility in men, male rats and     

THE EFFECTS OF TRIPTERYGIUM WILFORDII ON MALE REPRODUCTION

The multiglycosides of Tripterygium wilfordii (GTW) has been used in China for the treatment of rheumatoid arthritis, chronic nephritis and certain skin diseases.     

PROTECTIVE EFFECT OF INSULIN ON ISOLATED RAT HEARTS

The protective effect of preoperative intravenous infusion of high doses of insulin (2U/100gm) was studied in isolated rat heart models, using modified Langendorff apparatus. Fifteen rat hearts were equally divided into three groups: Group Ⅰ(control group) received glucose and potassium within 60 min. prior to the onset of operation. Group Ⅱ (insulin group) was administered with glucose, insulin and potassium(GIK)within 60 min. before operation, whereas Group Ⅲ(insulin cardioplegia group)was treated with the same amount of GIK Preoperatively as well as an addition of insulin (100U/L) to the cardioplegia. All the rat hearts were subjected to a 30-min. period of ischemia. Coronary effluent, lactic dehydrogenase (LDH) of coronary effluent, myocardial water content, histochemistry and ultrastructure of myocardium were studied. After reperfusion, the amount of coronary effluent was 2.84±0.79 ml/min/100gm in Group Ⅱ, 1.32±0.45 ml/min/100gm in Group Ⅰ and 1.72±1.03 ml/min/100gm in Group Ⅲ, respectively, (Group Ⅱ vs Group Ⅰ or Ⅲ p<0.05), LDH was significantly lower in Group Ⅱ (4.48±1.24 U/L) than in Group Ⅰ (25.16±8.96 U/L) (P<0.01) and in Group Ⅲ (17.8±12.2 U/L) (P<0.05), but no significant difference could be seen between Group Ⅰ and Group Ⅲ. Myocardial water content was 71.5±1.21% in Group Ⅱ and 78.57±3.14% in Group Ⅰ (p<0. 01), whereas it was 75.42±3.28% in Group Ⅲ(vs Group Ⅰ and Ⅱ p>0.05).Histochemical succinodehydrogenase (SDH) reaction was graded 0 to 1 in Group Ⅱ and 2 to 3 in Group Ⅰ and Ⅲ (p<0.01). Group Ⅱ showed nearly normal myocardial ultrastructure with increased glycogen stores, while Groups Ⅰ and Ⅲ revealed local myofibrillary disorganization. disrupted mitochondria and Pronounced depletion of glycogen storages. These results prove that the Protective effect of preoperative application of high doses of insulin together with glucose and potassium on isolated rat hearts is definitely superior to the use of glucose and potassium alone. These findings also suggest that addition of insulin to cardioplegia is ineffective and even harmful to the ischemic myocardium.     

EFFECT OF MULTIGLYCOSIDES OF TRIPTERYGIUM WILFORDH ON THE FUNCTION OF LEYDIG CELLS

Animal experiments and clinical researclies showed that multiglycosides of Tripterygium wilfordii (GTW) had male contraceptive effects, At contraceptive doses, GTW did not significantly affect the plasma level of testosterone, but higher doses of GTW did decrease     

柳氮磺胺吡啶对大鼠生精功能及精子形态的影响

用流式细胞术测定大鼠睾丸生精过程中各种细胞的相对数量及ＤＮＡ含量变化，并取大鼠附睾尾部精子进行扫描电镜观察。发现柳氮磺胺吡啶（ＳＺ）以３５０ｍｇ／ｋｇ·ｄ－１用药２个月后，可轻度抑制睾丸非生精细胞．使增殖活跃细胞ＤＮＡ含量更趋一致。，但不干扰精子的生成，也不会引起精子遗传物质的改变。精子的颈部结构发生明显破坏性改变，断头精子显著增多（Ｐ＜０．０１）。实验结果显示：ＳＺ对睾丸生精过程无明显干扰，但显然能通过某些途径导致精子形态异常。     

雷公藤对小鼠睾丸影响的酶组织化学观察

本实验用酶组织化学方法,观察服用雷公藤82糖浆和正常对照组小鼠睾丸组织中几种酶的变化。结果显示:给药6个月,曲精小管上皮的各级生精细胞中琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、三磷酸腺苷酶(ATPase)活性减弱;支持细胞中酸性磷酸酶(ACP)活性增强;碱性磷酸酶(AKP)活性,在给药组与对照组间无明显差别;睾丸间质细胞中SDH、ACP、AKP、ATPase等酶活性变化均不明显,但LDH活性增强。提示:雷公藤可抑制生精细胞中主要酶系的活性,进而导致精子发生和成熟的障碍。本文还就支持细胞中ACP活性增强做了讨论。     

微波辐射对大鼠附睾精子的影响

结扎大鼠双侧睾丸输出管,屏蔽一侧阴囊,另一侧阴囊用微波辐射,并使其附睾充分暴露于微波辐射场内。微波辐射后,于立即、1、4、7、15及28天分别观察双侧附睾尾部精子,结果表明:辐射侧与对照侧前向运动精子百分率、运动精子百分率及尾部精子总数均逐渐减少,而断头精子百分率则逐渐增加;除尾部精子总数外,各观察项目辐射侧改变较对照侧早出现3～12天;与对照侧相比,辐射侧在第4天即出现显著性差异。提示,微波辐射可以干扰精子在附睾的成熟和贮存。     

雷公藤多甙(GTW)抗雄性生育活性的研究

目的 :研究雷公藤多甙 (GTW )的抗雄性生育活性机制。方法 :给Wistar雄性大鼠灌服GTW 8周后 ,观察其睾丸、附睾形态及一氧化氮合成酶活性的变化。结果 :睾丸内的精子细胞及精子减少、曲细精腔缘出现病理形态的细胞及多核巨细胞。附管内可见精子断头及脱落细胞 ,睾丸间质细胞一氧化氮合成酶平均密度下降。结论 :雷公藤多甙的雄性抗生育作用对精子细胞及睾丸间质细胞等有影响。     

雷公藤多甙治疗多发性肌炎的临床研究

应用雷公藤多甙（ＴⅡ）治疗铮性怀肌炎７例，其中４例仅用于ＴⅡ治疗，另３例无关在足够部程的大剂量强的松治疗无效，并将强的松碱量至极小维持量地用ＴⅡ治疗，该７例患乾症状均消失，体征恢复到正常，肌酶谱降至正常范围。结果显示，ＴⅡ不仅对多发性肌炎有良好的疗效，而且对皮质类固醇治疗无效的病例仍有治疗作用。ＴⅡ治疗多发性肌炎的疗效与其免疫抑制作用密切相关。