5-氨基酮戊酸光动力疗法治疗中重度痤疮的临床疗效研究

目的观察5-氨基酮戊酸光动力(ALA-PDT)联合丹参酮胶囊治疗中重度痤疮的效果。方法将90例痤疮患者随机分为三组,每组30例,进行口服加外用药物疗法、单纯ALA光动力疗法和ALA光动力联合丹参酮胶囊治疗。治疗结束后1个月进行疗效比较。结果口服加外用药物组总有效率为46.7%;单纯ALA光动力疗法组总有效率为76.7%;ALA光动力联合丹参酮胶囊治疗组总有效率为83.3%。三组之间有效率比较有显著性差异(P 0.05)。口服加外用药物组和ALA光动力组比较,两组之间有效率有显著性差异(P 0.05)。ALA光动力组与ALA光动力联合丹参酮组比较,两组之间有效率无显著性差异(P 0.05)。口服加外用药物组和ALA光动力联合丹参酮组比较,两组之间有效率有显著性差异(P 0.05)。结论 5-氨基酮戊酸光动力治疗中重度痤疮临床疗效优于口服外用药物治疗方法,但与光动力联合丹参酮胶囊疗法比较并无显著性差异。     

多功能电离子联合ALA-PDT及干扰素治疗宫颈尖锐湿疣疗效评价

目的:评价多功能电离子联合5-氨基酮戊酸光动力及重组人干扰素肌肉注射治疗宫颈尖锐湿疣的疗效。方法:将240例宫颈尖锐湿疣患者随机分为4组。A组多功能电离子烧灼治疗后行ALA-PDT治疗,每周1次及重组人干扰素50μg肌肉注射,每周3次,连续4周。B组电离子烧灼后行ALA-PDT治疗,每周1次,共4次;C组多功能电离子烧灼后肌注重组人干扰素,每周3次,连续4周;D组仅使用多功能电离子烧灼。所有患者末次治疗结束后每月复诊1次,连续3次。并于末次治疗结束后的第4、8和12周进行复发率评估。结果:共189例患者完成治疗和随访。随访3个月后,A、B、C、D各组患者痊愈率分别为96.08%,83.67%,81.25%及53.66%,各组HPV清除率分别为74.51%,53.06%,50.00%及12.20%。结论:多功能电离子联合5-氨基酮戊酸光动力及重组人干扰素肌肉注射治疗宫颈尖锐湿疣的疗效满意。     

火针联合5-氨基酮戊酸光动力治疗面部难治性扁平疣疗效观察

目的观察火针联合5-氨基酮戊酸光动力治疗面部难治性扁平疣临床疗效。方法符合面部难治性扁平疣诊断的患者90例,随机分为A、B、C 3组,每组30例。A组采用火针治疗,B组采用5-氨基酮戊酸光动力治疗,C组采用火针联合5-氨基酮戊酸光动力治疗。比较3组患者治疗前后皮损评分及临床有效率。随访1年,统计复发率。结果治疗前皮损评分A组(6.25±2.06)分,B组(6.21±2.13)分,C组(6.23±2.09)分,治疗后皮损评分为A组(3.17±0.93)分,B组(3.10±1.07)分,C组(1.92±0.81)分。3组治疗后皮损评分与治疗前相比,差异均有统计学意义(P 0.05);治疗后C组评分与A、B组比较,差异均有统计学意义(P 0.05)。A、 B、C 3组有效率分别为76.7%、73.3%和90.0%。C组与A、B两组比较,差异均有统计学意义(P 0.05)。A、B、C3组复发率分别为30.4%、31.8%和11.1%。C与其他两组比较,差异有统计学意义(P 0.05)。结论火针联合5-氨基酮戊酸光动力治疗颜面部难治性扁平疣具有安全有效、复发率小的临床效果。     

光动力疗法和咪喹莫特预防老年男性尖锐湿疣复发的疗效观察

目的比较5-氨基酮戊酸光动力疗法(ALA-PDT)和咪喹莫特对老年男性尖锐湿疣患者经微波治疗后疾病复发率的影响。方法将92例老年尖锐湿疣患者随机分为三组,微波治疗组(A组)、微波联合ALA-PDT组(B组)和微波联合咪喹莫特组(C组)。所有患者予微波祛除疣体,A组34例在创面愈合以后局部不给任何药物治疗;B组26例在原疣体部位及周边1cm范围局部外敷20%5-氨基酮戊酸(ALA)霜剂,并用薄膜封包3h,再用光动力治疗仪照射,时间为20min/光斑,1次/周,连续3次;C组32例创面愈合以后外用咪喹莫特软膏,隔天1次,连用12周。随访6个月,观察复发率及不良反应。结果微波联合ALA-PDT组和微波联合咪喹莫特组治疗后复发率分别为15.38%和21.88%,显著低于微波治疗组(52.94%)(P0.01),但前两者间复发率差异无统计学意义(P0.05)。结论 ALAPDT和咪喹莫特均能有效降低老年男性患者尖锐湿疣微波治疗后的复发,且效果相似。     

电离子联合5-氨基酮戊酸光动力治疗尖锐湿疣的临床疗效及安全性分析

目的:探讨电离子联合5-氨基酮戊酸光动力治疗尖锐湿疣的临床疗效及安全性。方法:选择2013年6月至2014年3月期间本院诊治的100例尖锐湿疣患者为研究对象,根据治疗方式的不同将患者分为对照组(50例)与观察组(50例)。对照组患者接受单纯电离子治疗,观察组患者接受电离子联合5-氨基酮戊酸光动力治疗。比较两组临床疗效、复发率、疣体清除数及不良反应发生率。结果:观察组与对照组痊愈率分别为98.0%和72.0%,差别具有统计学意义(P0.05);随访3月,观察组复发率显著低于对照组(12.0%vs 30.0%,P0.05);观察组疣体清除数显著多于对照组,差别具有统计学意义(P0.05);两组不良反应发生率分别为20.0%和64.0%,差别具有统计学意义(P0.05)。结论:与单纯电离子治疗相比,电离子联合5-氨基酮戊酸光动力治疗尖锐湿疣临床疗效更为理想,可减少复发率且安全性好。     

不同浓度5-氨基酮戊酸光动力疗法治疗面部泛发性扁平疣临床观察

目的通过比较不同浓度5-氨基酮戊酸(ALA)光动力治疗的临床疗效,分析影响面部泛发性扁平疣的疗效和安全性。方法将泛发性扁平疣患者面部皮损分为左右两个侧面,随机给予5%、10%、20%ALA霜剂,局部点涂用药,每个治疗组均给予红光照射,累积照射剂量为339 J/cm2,治疗4、8、12周后分别计算皮损清除率及不良反应。结果共55例患者入组,3种浓度组在4、8、12周时皮损总清除率分别为74.1%、68.8%、64.6%。12周后,5%ALA组平均痊愈率(14.3%)显著低于10%ALA组(33.3%,P0.05)及20%ALA组(26.3%,P0.05),治疗期间平均疼痛指数为3.8分。12周后的皮损复发率为16.7%(4/24),色素沉着的发生率为69.8%(67/96)。4、8、12周后20%浓度组色素沉着发生率均显著高于其他两组。结论光动力治疗泛发性扁平疣疗效好,不良反应低,安全性高。10%ALA组的疗效、安全性优于5%、20%ALA组。     

注射氨基酮戊酸光动力疗法对大鼠痤疮样炎性结节模型的疗效及组织病理学影响

【摘要】 目的 探讨注射氨基酮戊酸（ALA）光动力疗法对大鼠痤疮样炎性结节模型的疗效及组织病理改变。方法 40只SPF级SD大鼠分为正常对照组、模型对照组、注射ALA组、外敷ALA组，每组10只。除正常对照组不处理外，其余3组大鼠右耳廓内侧接种痤疮丙酸杆菌，建立大鼠痤疮样炎性结节模型。造模成功后，模型对照组不处理，注射ALA组将5%ALA注射入结节内再予红光照射，外敷ALA组直接外敷5%ALA于鼠耳痤疮样结节处再予红光照射，均为每周1次，共2次。末次治疗24 h后观察各组大鼠耳部大体表现和组织病理学变化，测量耳廓厚度，并检测肝肾功能。采用单因素方差分析和LSD-t检验比较各组差异。结果 正常对照组、模型对照组、外敷ALA组、注射ALA组大鼠耳廓厚度分别为（0.435 ± 0.006）、（1.269 ± 0.071）、（1.088 ± 0.098）、（0.699 ± 0.095） mm，差异有统计学意义（F = 235.60，P < 0.001），模型对照组耳廓厚度高于正常对照组、外敷ALA组和注射ALA组（t 值分别为24.18、5.24、16.48，均P < 0.01）；注射ALA组低于外敷ALA组（t = 11.24，P < 0.01）。外敷ALA组和注射ALA组大鼠耳廓局部红肿程度、结节数均低于模型对照组，真皮及皮下组织浸润炎症细胞数量减少，注射ALA组结节消退更明显，亦未见团块状分布的炎症细胞或微脓肿形成。各组大鼠丙氨酸转氨酶、天冬氨酸转氨酶、肌酐、尿素氮水平差异均无统计学意义（均P > 0.05）。结论 注射ALA光动力疗法治疗大鼠痤疮样炎性结节模型较外敷ALA光动力疗法治疗更有效。     

解毒痤疮丸联合5-氨基酮戊酸光动力治疗中重度痤疮疗效观察

目的观察解毒痤疮丸联合5-氨基酮戊酸光动力治疗中重度痤疮的疗效。方法治疗组50例采用解毒痤疮丸联合5-氨基酮戊酸光动力治疗,对照组50例单用5-氨基酮戊酸光动力治疗。结果治疗组有效率为96%,对照组有效率为80%,2组比较差异有统计学意义(P0.05)。结论解毒痤疮丸联合5-氨基酮戊酸光动力治疗中重度痤疮有良好的治疗效果。     

5-氨基酮戊酸光动力疗法治疗复发性尖锐湿疣65例疗效观察

目的观察5-氨基酮戊酸光动力疗法(ALA-PDT)治疗复发性尖锐湿疣的临床疗效及复发率。方法65例复发性尖锐湿疣患者局部外敷5-盐酸氨酮戊酸(ALA)后,用波长635nm的氦氖激光照射疣体,每次20～40min,1次/ w,共治疗1～6次;对照组为55例初发尖锐湿疣患者,用传统CO_2激光常规治疗。比较两组的疗效及复发率。结果治疗组经1～6次治疗后疣体完全消退,无溃疡、尿道狭窄、感染等并发症,术后随访3个月复发7(10.77%)例。对照组经1～2次治疗后疣体全部清除,术后随访3个月复发26(47.27%)例,其中19(34.55%)例在1～3周内复发。结论ALA -PDT治疗尖锐湿疣疗效可靠、复发率低、耐受性好。     

ALA-PDT联合2940nm铒点阵激光治疗中重度痤疮疗效分析

目的探讨ALA(5-氨基酮戊酸)外敷凝胶光动力疗法联合点阵激光治疗中重度痤疮的有效性及安全性。方法选取30例Ⅱ~Ⅳ级面部痤疮患者,随机分为两组。治疗组采用2 940nm铒点阵激光治疗后,立即5%浓度ALA外敷封包后运用光动力红光照射面部,对照组单纯采用5%浓度ALA外敷封包后运用光动力红光照射面部,外敷药物封包时间均为2.5h,照光能量密度80mw/cm~2,照光时间20min,15d治疗1次,共治疗3次。结果 ALA联合点阵激光治疗1次、2次、3次的有效率分别为0,93.33%和100.00%;单独ALA治疗1次、2次、3次的有效率分别是0.00%,60.00%和100.00%。结论 5%ALA-PDT联合点阵激光是治疗中重度痤疮都能使痤疮简单、有效而又安全的疗法。     

聚乙交酯丙交酯纳米粒装载氨基酮戊酸光动力疗法对A431细胞的杀伤效应

【摘要】 目的 制备装载氨基酮戊酸（ALA）的聚乙交酯丙交酯纳米粒（PLGA NP）,增强ALA光动力体外杀伤人皮肤鳞癌A431细胞的效应。方法 改良复乳溶剂挥发法制备ALA PLGA NP,并对其粒径、包封率、载药量和形态进行表征。用透射电镜观察体外培养的A431细胞吸收ALA PLGA NP后的形态;用多功能酶标仪测定24 h内0.1 mmol/L ALA组、1 mmol/L ALA组、ALA PLGA NP组（含0.1 mmol/L ALA）、PLGA NP组生成的原卟啉IX（PpIX）荧光强度变化以确定最佳孵育时间。将培养A431细胞分为对照组、0.1 mmol/L ALA避光组、1 mmol/L ALA避光组、ALA PLGA NP避光组、PLGA NP避光组、单纯照光组、0.1 mmol/L ALA PDT组、1 mmol/L ALA PDT组、ALA PLGA NP PDT组、PLGA NP PDT组,对照组及各避光组严格避光,PDT组及单纯照光组用He-Ne激光照射,用噻唑蓝法测定其细胞杀伤效应。将A431细胞分为对照组、ALA PDT组、ALA PLGA NP PDT组,对照组避光,PDT组采用He-Ne激光照射,12、24 h后用流式细胞仪分析细胞凋亡情况。 结果 ALA PLGA NP呈球形,平均粒径为（65.6 ± 26.0） nm,包封率为（65.8 ± 7.2）%,载药量为（0.62 ± 0.27）%。ALA PLGA NP能被A431细胞吸收并聚集于细胞质中。PpIX荧光动力学检测显示24 h内ALA组、ALA PLGA NP组荧光强度随时间增加而上升。当孵育6 h、24 h时,ALA PLGA NP组生成的PpIX荧光强度均高于0.1 mmol/L ALA（均P < 0.01）。ALA PLGA NP PDT组对A431细胞的杀伤效应也明显强于0.1 mmol/L ALA PDT（6 h：t = 35.685,P < 0.01;24 h：t = 5.262,P < 0.01）。ALA PLGA NP PDT组细胞的凋亡率也高于ALA PDT组（12 h：t = 9.074,P < 0.01;24 h：t = 9.095,P < 0.01）。 结论 ALA PLGA NP能提高PpIX生成量,增强ALA PDT对A431细胞的体外杀伤效应以及ALA PDT诱导肿瘤细胞凋亡的效应。 【关键词】 肿瘤,鳞状细胞; 光化学疗法; 氨基酮戊酸; 纳米粒子     

Delta-aminolevulinic acid-based photodynamic therapy for acne on the body

Intractable acne on the body was clinically treated with photodynamic therapy (PDT) combining oral administration of delta-aminolevulinic acid (ALA) with polychromatic visible light from a metal halide lamp. The total number of body acne patients was 51. A 10 mg/kg B.W. of ALA was administered to the patients, and the acne lesions were exposed to the light emitted from the metal halide lamp. The wavelengths of the light ranged from 540 to 800 nm. The diameter of the irradiated area and the irradiating distance from the lamp were 100 mm and 130 mm, respectively. Under the irradiation conditions, the total fluence rate and the energy-density uniformity of the light were 69.2 mW/cm2 and 11.5%. The total light energy dose in one session was 60-80 J/cm2 for the body. All patients underwent two sessions of PDT and received no other treatments after PDT or during the follow-up period. The interval between the two PDT sessions was 2-4 weeks. The physician's clinical assessment scores as worsened, unchanged, improved, and markedly improved were 0 (0%), 4 (7.8%), 16 (31.4%), and 31 (60.8%) patients, respectively. Adverse effects of very slight discomfort, burning, and stinging were reported during the irradiation. The blood tests of patients before and after oral administration of ALA demonstrated that no liver dysfunction occurred in this study with ALA dosages within 10 mg/kg B.W. Consequently, orally administered ALA-PDT with a metal halide lamp was considered to be effective for treatment of acne on the body.     

The influence of temperature on 5‐aminolevulinic acid‐based photodynamic reaction in keratinocytes in vitro

Background/purpose: Based on the observation that increasing skin temperature could improve 5‐aminolevulinic acid (ALA) penetration and accumulation of protoporphyrin IX (PpIX) in the ALA‐based photodynamic therapy (PDT), this study was designed to investigate how temperature change varied the therapeutic effect of ALA‐based PDT in vitro. Methods: HaCat cells were cultured with or without ALA at various temperatures. ALA uptake and PpIX accumulation were analyzed before laser irradiation as the baseline. After irradiation, cell death and cytokine secretions in the media, including interleukin (IL)‐1α, tumor necrosis factor (TNF)‐α and basic fibroblast growth factor (bFGF) were assayed, and the morphological changes were recorded. Results: With increasing temperature, the amount of ALA uptake, intracellular PpIX concentration and cell death increased in both the PDT and the non‐PDT groups. Secretions of IL‐1α, TNF‐α and bFGF also increased and reached a peak around 44–47 °C and then declined at a higher temperature. This biphasic response might be due to protein thermolysis that occurs when cells reach beyond thermal tolerance. Conclusions: Elevating temperature could augment photodynamic reactions to a certain extent, but adverse effects occurred when cells were overheated.     

A pilot study to assess the efficacy of photodynamic therapy for Japanese patients with actinic keratosis in relation to lesion size and histological severity

Background/purpose: Topical 5-aminolevulinic acid (ALA)-based photodynamic therapy (PDT) is effective for actinic keratosis (AK); few studies have examined Oriental patients. The aim of this study is to assess the efficacy of PDT for the treatment of Japanese AK patients classified by lesion size and histological severity.
Methods: Thirty patients with solitary AK lesions were divided into two groups according to diameter: a small lesion group (SL), diameter ≤10 mm and a larger lesion group (LL), diameter >10 mm, and histological severity: Group I (mild and moderate) and Group II (severe). After application of 20% ALA for 4 h, exposure to an excimer-dye laser at 630 nm was performed at a dose of 50 J/cm² three times at an interval of 7 days. Therapeutic effects were assessed and followed for 12 months.
Results: In all 10 SL patients, atypical cells disappeared after PDT and did not recur for 12 months. However, for the 20 LL patients, recurrence was seen in 2 of the 14 Group I patients, while 4 of 6 Group II patients showed residual tumor cells after the first PDT session.
Conclusion: The present study demonstrated that ALA-PDT might be useful for treatment of Japanese AK. The therapeutic outcome might depend on the lesion size and the histopathological severity.     

光动力疗法治疗乳房外Paget病临床研究

目的 探讨荧光诊断指导下光动力治疗肛周皮肤湿疹样癌的临床意义。方法 肛周Paget病6例,前期3例行普通光动力治疗(PDT),后期3例行荧光诊断指导下光动力治疗,普通PDT组给以光敏剂Photofrin 2mg/kg,48h后给以功率100～150mW /cm2、能量密度150～300J/cm2,波长630 nm半导体激光照射病灶;荧光诊断指导PDT组（FPDT）给以局部20％ALA湿敷（激光照射前3h）和静脉给光敏剂喜泊分5mg/kg（激光照射前48h）,并给以同样剂量激光照射,激光照射前给以405nm蓝紫光行荧光诊断确定病灶的范围以指导治疗。治疗后48h和72h, 1、3和6个月观察患者病灶局部反应并长期随访。结果 治疗后48h 2组均可见病灶局部明显变暗、发黑、变硬,120h局部开始结痂,渗出减少,皮肤修复时间长。治疗后6个月普通PDT组CR1例,PR2例;FPDT组CR2例,PR1例。结论　光动力学疗法是肛周Paget病的有效治疗方法,荧光诊断对于确定病灶范围、指导临床治疗意义重大。     

Therapeutic and immune effects of 5‐aminolevulinic acid photodynamic therapy on UVB‐induced squamous cell carcinomas in hairless mice

The therapeutic effects of 5‐aminolevulinic acid (ALA)‐mediated photodynamic therapy (PDT) on cutaneous squamous cell carcinoma (SCC) are not fully understood, and the usefulness of topical PDT in the treatment of SCC is still debatable. The most interesting aspect in SCC PDT is perhaps its potential in inducing antitumor immune responses. In this study, cutaneous SCCs were established by UVB irradiation of hairless mice and treated with multiple ALA PDT. Immunohistochemistry assays showed that ALA PDT could induce quick apoptosis, overexpression of TNFα and marked increases in DCs, CD4⁺ and CD8⁺ cells in tumor interstitium and subcutaneous connective tissues. However, a complete response was only achieved for small SCCs. The clinical value of ALA PDT‐induced specific antitumor immune responses in long‐term control of SCCs deserves further study.     

光动力疗法对人宫颈癌Caski细胞裸鼠移植瘤MIF表达的影响

目的:探讨光动力疗法对人宫颈癌Caski细胞裸鼠移植瘤巨噬细胞移动抑制因子(MIF)表达的影响。方法:选取BALB/c裸鼠,皮下注射人宫颈癌Caski细胞,10d后将达到荷瘤标准的40只裸鼠采用随机数字表法分为四组,即A组、B组、C组和D组,各10只。A组作为阴性对照、B组给予单次光动力疗法、C组给予多次光动力疗法和D组给予顺铂腹腔注射。对比治疗前和治疗2周后肿瘤体积,治疗前后裸鼠体质量、瘤体积、瘤质量变化及抑瘤率,并分别采用western-blot和免疫组化法对各组裸鼠肿瘤组织MIF表达情况进行检测,行对比分析。结果:B组、C组和D组体质量、瘤体积和瘤质量变化和A组比较差异均有统计学意义(P<0.05),且C组和D组上述指标及抑瘤率和B组比较差异均有统计学意义(P<0.05),而C组和D组间各项指标差异均无统计学意义(P>0.05);电镜分析结果显示各组肿瘤胞浆可见明显空泡,团间有明显坏死灶,并且随着光动力疗法剂量增高,坏死区域增大越明显。B组、C组和D组MIF蛋白IOD值均较A组显著降低(P<0.05),且C组和D组MIF蛋白相对表达量和IOD值均较B组显著降低(P<0.05),而C组和D组MIF蛋白相对表达量和IOD值比较差异均无统计学意义(P>0.05)。结论:采用多次光动力疗法对人宫颈癌Caski细胞裸鼠移植瘤效果和顺铂相近,且可显著降低MIF表达水平。     

A randomized, double-blind, placebo-controlled study of the efficacy of tetracaine gel (Ametop) for pain relief during topical photodynamic therapy

BACKGROUND: Many patients find topical 5-aminolaevulinic acid (ALA) photodynamic therapy (PDT) painful. Local anaesthetics are not routinely used and their effect on PDT pain has not been examined. OBJECTIVES: To evaluate the efficacy of tetracaine gel (Ametop) for pain relief during and after PDT. METHODS: A prospective, double-blind, placebo-controlled study of 42 patients with lesions (< or =2 cm diameter) of superficial nonmelanoma skin cancer or dysplasia. Patients were randomized to either tetracaine (4% w/w) (n=22) or vehicle (n=20) gel under occlusion for 1 h pre-irradiation. Pain was assessed during and after irradiation using a visual analogue scale (VAS) and faces pain scale. RESULTS: Patients who received tetracaine gel experienced only slightly less pain during PDT (median VAS 4) compared with those who received placebo (median VAS 4.5) (95% confidence interval for difference 0-3, P=0.08). No significant difference in pain was experienced between the treatment groups immediately after irradiation or later. CONCLUSIONS: When compared with placebo, tetracaine gel did not significantly reduce pain during or after PDT for small lesions of superficial basal cell carcinoma, Bowen's disease or actinic keratosis.     

In vivo measurement of 5-aminolaevulinic acid-induced protoporphyrin IX photobleaching: a comparison of red and blue light of various intensities

BACKGROUND: In recent years, 5-aminolaevulinic acid (ALA) has become an increasingly popular photosensitizing drug for use in both photodynamic therapy (PDT) and photodetection (PD) of cancers. ALA metabolizes within tissue to form the photosensitizer protoporphyrin IX (PpIX). Like most photosensitizers, PpIX is fluorescent, and this fluorescence progressively decreases during PDT. This phenomenon is referred to as photobleaching. AIM: Our aim in carrying out this experiment was twofold: firstly, to compare the relative capacity of red and blue light to cause photobleaching; and secondly, to compare the capacity of a fixed light dose to cause photobleaching, when delivered at different intensities. METHOD: In this paper, we describe the implementation of a compact fluorescence spectrometer in monitoring the photobleaching of ALA-induced PpIX in vivo on the skin of healthy volunteers. RESULTS: We have been able to show that blue light causes more rapid photobleaching than red light, and that under illumination with red or blue light, delivery of a fixed light dose at a lower intensity results in more photobleaching. CONCLUSION: Comparison of the photobleaching rates suggests that a blue light intensity of 5 mW/cm(2) gives the same rate of photobleaching as the typical red light PDT intensity of 100 mW/cm(2). Further investigation of the correlation between PpIX photobleaching and PDT effect would be beneficial in interpreting the clinical significance of our findings.     

不同剂量ALA-PDT对皮肤鳞状细胞癌A431细胞增殖的抑制作用

目的:确定5-氨基酮戊酸-光动力疗法对皮肤鳞状细胞癌A431细胞抑制的最佳剂量。方法:体外培养人皮肤鳞状细胞癌A431细胞株,应用MTT法检测不同ALA浓度(0.5、1、2 mmol/L)、不同PDT光照剂量(5、10、20、40 J/cm~2)下A431细胞的增殖水平。结果:当ALA浓度为0.5 mmol/L时,光照剂量为5、10、20、40 J/cm~2对A431细胞的抑制率分别为6%、10%、15%、18%;当ALA浓度为1mmol/L时,光照剂量为5、10、20、40 J/cm~2对A431细胞的抑制率分别为30%、52%、80%、82%;当ALA浓度为2 mmol/L时,光照剂量为5、10、20、40 J/cm~2对A431细胞的抑制率分别为31%、54%、81%、82%。结论:ALA在浓度为1 mmol/L,光照剂量为20 J/cm~2时,ALA-PDT对A431细胞增殖抑制效果达到最佳。