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甘草素与异甘草素分离研究 总被引:2,自引:0,他引:2
甘草素、异甘草素系甘草中黄酮类化合物,由于它对艾滋病病毒的增殖有抑制作用,故在临床应用及分离手段方面受到普遍重视。因其在甘草中含量较少,国外一般采用高效液相色谱法分离,而国内采用柱层分离法。本文对其薄层条件进行实验探讨。 相似文献
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对异甘草素的合成工艺进行优化和体外清除自由基活性进行研究。通过间苯二酚、冰醋酸、对羟基苯甲酸合成异甘草素,考察物料比、温度、反应时间对产率的影响,通过紫外分光度法对其清除自由基活性进行研究。酚与酸物料比1∶2.5,240 W微波辐射3 min,2,4-二羟基苯乙酮产率最高为65.75%;当醛酮物质的量比为3∶1,温度控制在120~130℃,回流6 h,异甘草素产率最高为28.10%;反应时间控制20 min,异甘草素加入量为1.00 m L清除DPPH自由基能力最强,为66.4%。异甘草素具有一定的抗自由基活性。 相似文献
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薄层色谱法在α-苯乙醇合成中的应用 总被引:1,自引:0,他引:1
用薄层色谱法对α-苯乙醇合成的产品进行了分离、分析。选择出最佳展开溶剂体系为:己烷:乙酸乙酯=5:3(v/v),用该展开剂可有效地分离反应原料和反应产物。并用柱色谱分离得到了纯的产品。 相似文献
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正交实验优化异甘草素超声提取工艺 总被引:2,自引:0,他引:2
采用超声法提取乌拉尔甘草中异甘草素,通过乙醇体积分数、液固质量体积比(g/mL)、超声提取时间和提取次数4个因素对异甘草素提取率的影响进行了正交实验,确定超声提取最佳工艺条件为:体积分数80%乙醇,液固质量体积比(g/mL)10∶1,超声20 m in,提取3次,异甘草素的提取率为0.37‰,是乙醇热回流提取的2.06倍,是索氏提取的1.54倍。超声提取浸膏中异甘草素的质量分数为0.55%,是乙醇热回流提取的3.24倍,是索氏提取的1.45倍。结果表明:超声提取甘草中异甘草素具有提取温度低、速度快、提取率高、纯度高等特点。 相似文献
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以10μm硅胶为基质,异甘草素为配体,采用液相连续反应法及中间体法依次合成了异甘草素键合硅胶固定相ISLSP-Ⅰ和ISLSP-Ⅱ。采用FTIR及SEM对合成的固定相进行表征。将ISLSP-Ⅰ固定相填充于50 mm×10 mm i.d. 1号色谱柱中,将ISLSP-Ⅱ固定相填充于30 mm×10 mm i.d. 2号色谱柱中,系统评价了两种方法合成的固定相的色谱性能。结果表明:两根色谱柱的柱效及峰型均较好,大流速下测试组分的保留主要决定于传质阻力。在甲醇-水体系下,2号色谱柱表现出明显的反相色谱分离机制。合成的固定相稳定性良好,使用6个月后,测试组分在两根色谱柱上的保留时间相对偏差不超过0.9%。2号色谱柱对多组分标准样品及中药提取物均具有良好的分离能力。 相似文献
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薄层色谱法的诞生与发展,在我国的药物分析领域中是革命性的,它改变了传统药品质量控制中只凭性状、经验来把握质量的单一模式,使药的质量控制、质量保证及药物作用的理解一步进入到可查的分子水平。本文主要探讨了薄层色谱法在药品分析中的应用。 相似文献
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O. S. Privett M. L. Blank D. W. Codding E. C. Nickell 《Journal of the American Oil Chemists' Society》1965,42(5):381-393
Techniques for the quantitative analysis of lipids using thin-layer chromatography (TLC) are reviewed. The general procedures
are divided into two groups on the basis of whether or not the methods involve the recovery of substances from chromatoplates.
Recovery methods are elaborated under detection of spots, recovery of substances and quantification. Methods are described
for the recovery of labile compounds from chromatoplates and for the determination of the structures of triglycerides and
lecithins.
Methods for the direct quantitative analysis of spots on chromatoplates are reviewed. These include measurements of spot size,
reflectance, absorbance of transmitted light, and fluorescence. Details of the photodensitometric method, particularly, spot
visualization and instrumentation are described. The analysis of lipid classes using a combination of DEAE cellulose chromatography
and TLC by the densitomery of charred spots is illustrated. 相似文献
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A method for the analysis of fatty esters employing separation by thin-layer chromatography and quantitative determinationvia their hydroxamic acids has been developed. Esters of different types are separated on silica gel plates, the spots or zones
are scraped from the plates, the esters are extracted from the silica gel, and the iron hydroxamic acid complexes are formed.
The latter are then measured by colorimetry.
The method is suitable for analysis of mixtures of ordinary, epoxy, monohydroxy, and dihydroxy fatty esters as well as for
mixtures of mono-, di-, and triglycerides.
Used in conjunction with gas chromatography this method permits the fatty acid composition of seed oils containing oxygenated
fatty acids to be measured in about 3 hours. The amount required for the total analysis is 1 to 10 mg. per determination,
depending upon composition.
Presented at the Spring Meeting, American Oil Chemists' Society, St. Louis, May, 1–3, 1961.
Supported by the Hormel Foundation, the National Institutes of Health (Research Grant H-3559), and the National Dairy Council.
Appointment supported by the International Cooperation Administration under the Visiting Research Scientists Program of the
U.S.A. 相似文献
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Quantitative analysis of lipids by thin-layer chromatography 总被引:1,自引:1,他引:0
M. L. Blank J. A. Schmit O. S. Privett 《Journal of the American Oil Chemists' Society》1964,41(5):371-376
A procedure is described for the quantitative analysis of neutral and phospholipids by thinlayer chromatography (TLC) employing
densitometry. The chromatophates are prepared with the usual solvent systems. The spots are charred under standard conditions
and analyzed with a Photovolt Corp. densitometer equipped with a special stage designed for holding 20×20 cm chromatoplates.
Each spot on the chromatoplate gives a peak of density values which is used for quantitative analysis.
Radioactive lipids are analyzed by autoradiography by the densitometry of radiograms of chromatoplates developed from X-ray
films.
The precision of the method is demonstrated on model mixtures of mono, di-and triglycerides, neural and phospholipids and
C14 labeled lipids.
Results of the analysis of several samples of rat liver lipids compared closely to those obtained by silicic acid column chromatography.
Supported in part by U. S. Public Health Service. Research Grant HE-05375. 相似文献
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Nicholas Pelick T. L. Wilson M. E. Miller F. M. Angeloni J. M. Steim 《Journal of the American Oil Chemists' Society》1965,42(5):393-399
This report describes methods for the thin-layer chromatography (TLC) of lipids and some practical aspects of the methods.
In order to present some basis for choosing the correct powder for particular separations, some properties of several widely
used silica gel powders are compared. The effect of binder material such as calcium sulfate in silica gel is studied. The
three systems, silica gel as a polar phase, silver nitrate-impregnated silica gel, and reversed phase systems are described
with application to neutral lipids. Also included are the applications of TLC to the polar lipids, such as phospholipids,
cerebrosides, sulfatides, sphingomyelin and other glycolipids from various sources. The pitfalls and precautions involved
in these separations are discussed in detail. 相似文献
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A. E. Thomas III J. E. Scharoun Helma Ralston 《Journal of the American Oil Chemists' Society》1965,42(9):789-792
A method has been developed for the analysis of isomeric monoglycerides by thin-layer adsorption chromatography. Isomeric
monoglycerides are separated on silica gel impregnated with boric acid; a mixed solvent system is used. The interaction of
hydroxy compounds with boric acid allows the separation of 1- and 2-monoglycerides which cannot be resolved on silica gel
alone. In order to make the method quantitative, the resolved components are charred and their spot densities measured. Glycerol,
fatty acids, diglycerides, and triglycerides do not interfere. The method does not induce isomerization.
Presented at the AOCS meeting, Chicago, Illinois, October 1964. 相似文献
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Argentation thin-layer chromatography was investigated as a method of obtaining detailed compositional information about milk
fat. A modified argentation thin-layer chromatography procedure, developed to optimize the separation of the complex mixture
of total milk fat triacylglycerols, provided nine different groups of triacylglycerols, based on both the degree of unsaturation
and the total length of fatty acid groups. Fatty acid methyl ester (FAME) analysis was performed to determine the composition
of each band. Separation on the basis of chainlength was most pronounced among the fully saturated triacylglycerol groups,
as evidenced by the high level of C4:0 and C6:0 in bands 7 and 8, respectively. For the cis-monoenoic triacylglycerols, the separation of C4:0 and C6:0 was less distinct. The cis,cis dienes and other dienoic, trienoic, or tetraenoic species were principally observed in two bands of retention factor <0.08
on the chromatography plate. Minimal cross-contamination of bands was observed, with the exception of the lowest of the trisaturate
bands, band 7, in which trans-monoenes were found to be present. Three samples from different points of the New Zealand dairy season were separated by
argentation thin-layer chromatography, and their FAME distributions were measured. In addition to differences in the masses
of band extracts from these samples, levels of C10:0 and C12:0 in all bands, and levels of monounsaturates in the dienoic and trienoic bands, were found to differ. These changes were generally
consistent with a pattern of decreasing fat hardness over the November to March period of a typical dairy season. 相似文献
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Cholesteryl phytanate and triglycerides containing phytanic acid were separated from their normal fatty acid analogs by thin-layer
chromatography. The presence of the branched-chain fatty acid makes the lipid less polar, and this effect becomes more pronounced
as the number of phytanic acid residues in the triglycerides is increased. Phytanic acid was prepared from commercial phytol
by catalytic hydrogenation, followed by catalytic oxidation. It contained 5% pristanic acid. 相似文献
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Compound classes in coal liquids were investigated by thin-layer chromatography coupled with a flame ionization detector (t.l.c.-f.i.d.). Individual t.l.c. peaks for coal liquids were identified as paraffins, aromatics, polar compounds and more strongly polar compounds or asphaltenes in order of increasing Rf values by comparison with specific compounds separated previously by conventional liquid chromatography. Concentrations of compound classes for a series of samples obtained by varying the hydrogenation time were estimated and good agreement was found with the results obtained by the USBM-API 60 liquid chromatography procedure. By using the t.l.c.-f.i.d. method, the relation of distribution of compound classes to the reaction conditions of coal hydrogenation could be derived quantitatively with relative ease. 相似文献