Differential root responses in two cultivars of winter wheat (Triticum aestivum L.) to elevated ozone concentration under fully open‐air field conditions

The effect of elevated tropospheric ozone concentration [O₃] on root processes in wheat systems of different O₃ sensitivity is not well understood. Two wheat cultivars (cv. Y15 and YN19) with contrasting O₃ tolerance were grown in a fully open‐air O₃ enrichment platform for one season. We found that elevated O₃ (EO₃) (50% above the ambient O₃) significantly decreased the total biomass at all key growth stages and the yield of the O₃‐sensitive cultivar YN19 but did not affect those of the O₃‐tolerant cultivar Y15. EO₃ significantly decreased the root biomass of two wheat cultivars at the jointing and grain‐filling stages. EO₃ significantly decreased the root length, length density, surface area and volume of the two cultivars at the jointing stage but increased those of YN19 at the grain‐filling stage. EO₃ significantly increased the root activities (specific root respiration rates) of Y15 and YN19 at the jointing, heading and grain‐filling stages. EO₃ significantly decreased the contribution of fresh root respiration to soil respiration (CRS) of YN19 at the jointing stage but increased it at the heading stage; however, it did not change the CRS of Y15 at any growth stages. This study indicates that the effects of EO₃ on root morphology and activity varied among wheat cultivars, and suggest that we can breed O₃‐tolerant cultivars to maintain crop yield under higher [O₃] scenarios.     

Phenol metabolism of two cultivars of durum wheat (Triticum durum Desf.) as affected by ozone and flooding stress

To compare the phenolic responses under oxidative stressors, plants of two Italian cultivars of durum wheat (Claudio and Mongibello) were (a) exposed to ozone (O₃) (80 ppb, 5 hr/day for 70 consecutive days), with the aim to investigate the changes of phenolic compound contents in their leaves, or (b) flooded (seven consecutive days). Plants showed O₃-induced visible injury, but their photosynthetic performance was not affected by the pollutant. Specifically, Claudio showed a higher O₃ tolerance than Mongibello. The major value of the present study is undoubtedly the pioneering investigation of phenolic metabolism of durum wheat under O₃. We identified 12 foliar phenolic compounds in all leaf samples (i.e. controls, exposed to O₃ and flooded): ten phenolic acids, a flavanol (catechin hydrate) and a phenolic aldehyde (syringaldehyde). Overall, O₃ exposure resulted in accumulations of phenolic compounds, especially in Claudio. These responses can be likely considered a fine-regulated repair process that equipped Claudio stressed plants with an antioxidant system capable of scavenging oxidative stress. Different phenolic variations were found in flooded plants, suggesting that phenolic response to environmental constraints is stress specific. Our study confirms that investigations and characterization of specific phenolic profiles of crop cultivars under oxidative stress may be helpful in breeding programmes.     

Chromosomes responsible for sensitivity of embryo to abscisic acid and dormancy in wheat

The sensitivity of the embryo to abscisic acid (ABA) has been reported toplay an important role in seed dormancy. Using ditelocentric lines of wheatcv. Chinese Spring (CS, nondormant and ABA insensitive), F₂ seedsbetween monosomic lines of CS and a wheat line Kitakei-1354 (dormant,ABA sensitive) and deletion lines of CS chromosome 4A, germinability ofseeds and embryo-half seeds incubated in water and ABA were examined. The results indicated that the long arm of chromosome 4A carried majorgene(s) for the embryo sensitivity to ABA and dormancy. Chromosome2D might be also involved in the sensitivity to ABA.     

78份四川小麦育成品种(系)条锈病抗性鉴定与抗条锈病基因分子检测

四川省是小麦条锈菌新小种产生的重要地区之一,了解2016年以来四川小麦育成品种(系)对当前流行的条锈菌生理小种和致病类型的抗性水平以及明确其抗条锈病基因的分布状况,可为四川育种防控小麦抗条锈病和品种布局提供理论依据。本研究选择2个小种CYR32和CYR34对78份四川小麦育成品种(系)进行苗期鉴定,利用当前小麦条锈菌优势小种CYR32、CYR33、CYR34,以及贵22-14、贵农致病类群等混合菌进行成株期人工接种鉴定,并利用19个抗条锈病QTL和基因QYr.nwafu-4BL、Yr5、Yr10、Yr15、Yr17、Yr18、Yr26、Yr28、Yr29、Yr30、Yr36、Yr39、Yr41、Yr48、Yr65、Yr67、Yr78、Yr80和Yr81的分子标记对供试材料进行抗条锈病基因检测。结果表明,在78份供试材料的苗期鉴定中,对CYR32表现出抗性的有60份,占76.92%;对CYR34表现出抗性的有40份,占51.28%;同时对CYR32和CYR34表现抗性的有36份,占46.15%。78份小麦品种(系)在成株期均表现抗条锈病,其中绵麦835、蜀麦1743、蜀麦1829和蜀麦1...     

Adaptation of wheat rusts to the wheat cultivars in former Czechoslovakia

Summary In former Czechoslovakia virulence of rusts attacking wheat was studied since the sixties. Since the same time genes for resistance in the registered cultivars were identified. The role of Berberis and Thalictrum as alternate hosts for stem rust and leaf rust, respectively, was investigated as well. Determined changes of virulence in rust populations could only partially be ascribed to changes of resistance genes in the grown cultivars. Unnecessary genes for virulence had no negative effect on the fitness of the pathogen. All tested samples of aeciospores from barberries attacked rye, not wheat. None of Thalictrum species occurring in the Czech and Slovak Republics was found to host wheat leaf rust. However, the sexual stage of wheat stem rust and wheat leaf rust could be induced on Berberis vulgaris and Thalictrum speciosissimum, respectively. General epidemiological conclusions are drawn from the results and experience of the last 35 years.     

重金属Cu、Zn、Pb复合污染对紫色土壤酶活性的影响

土壤酶是反映土壤肥力的一个敏感生物指标,还可以在一定程度上反映土壤环境的优劣状况。采用室内培养试验研究了铜、锌、铅复合污染对土壤过氧化氢酶,转化酶和脲酶活性的影响。结果表明铜、锌对三种酶都有抑制作用,其中锌对三种酶有显著抑制效果,而铅在一定浓度下对三种酶有激发作用。铜、锌、铅复合污染对脲酶的影响表现出协同抑制负效应的特征;对转化酶却表现出一定的拮抗作用,尤其铅浓度较高时,拮抗作用较明显;对过氧化氢酶的影响主要随铜、锌浓度增加而降低,锌的抑制作用显著。从土壤酶角度看,铅对轻度锌和铜污染有改良作用,而对铜污染修复效果不显著。     

不同浓度瑞飞特对土壤微生物及土壤酶活性的影响

以瑞飞特为材料,考察不同浓度瑞飞特对土壤微生物及土壤酶活性的影响。结果表明,当瑞飞特质量分数为0.0004%和0.004%时,能促进土壤中细菌、放线菌、固氮菌的生长及土壤脲酶、蛋白酶、蔗糖酶、过氧化氢酶活性,但也抑制真菌生长;当质量分数达到0.04%时,会抑制细菌、真菌、放线菌、固氮菌的生长及壤脲酶、蛋白酶、蔗糖酶、过氧化氢酶的活性。     

酸性硫酸盐土壤对柑桔枝生物炭改良的响应

酸性硫酸盐土壤(ASS)作为华南地区典型低产田,其改良利用有重大意义。本研究通过盆栽试验,研究不同柑桔枝生物炭(BC)用量对ASS的改良效果。试验设5 个处理,4 个重复。分别为NPK（氮、磷、钾肥）;NPK+0.5%BC（生物炭与干土质量比）;NPK+1%BC;NPK+2%BC;NPK+4%BC。结果表明：生物炭的施入使土壤pH值和有机质同时提高且变化趋势较为一致。土壤总氮含量随着生物炭用量增加而升高,最多提高了15.1%,但其平均升幅降低。氨态氮从11.46 mg/kg 降至0.96 mg/kg,硝态氮从8.37 mg/kg 降至5.14 mg/kg。这在降低面源污染风险的同时可能导致作物生长高峰期供氮不足。产量的提高在4% BC出现了拐点,表明过量使用会造成减产。土壤细菌和放线菌数量持续增加,但真菌数量无显著相关性。本试验表明适量添加柑桔枝生物炭,能够改良ASS并获得较好的环境和农学效益。     

Physiological responses of six bread wheat and durum wheat genotypes to water stress

Summary The responses of six wheat genotypes to water stress were analysed. Soil moisture (H), leaf water potential (_w), photosynthesis (P_N), stomatal resistance (r_s) and transpiration (T) were measured during a water stress. The genotypes investigated differed in their stress avoidance (_w-H relationship) and their stress tolerance (P_N-_w and r_s-_w relationships). The most important differences observed concern the mechanisms of tolerance at low leaf water potential: two varieties, Haurani 27 and Baalback, can then maintain a high photosynthetic activity. These observations are in agreement with the drought resistance characteristics already known for these genotypes. Possible applications to wheat breeding are considered     

Identification of bread wheat,durum wheat and barley cultivars adapted to dry areas of Southern Italy

Summary Among the cultivars of bread wheat, durum wheat and barley grown in the South of Italy, genetic variation for adaptation to the high temperature and drought stress conditions typical of the Mediterranean environment has been found.The basic data have been extrapolated from 5 years of Italian national network cultivar trials, where 20–30 cultivars were grown in replicated plot trials in 30–50 locations per year, including some where stress strongly affected grain yield.After careful identification of the most representative years and testing sites it was possible to characterise the cultivars on the basis of the grain yield in stress conditions and the Fischer & Maurer (1978) susceptibility index and to find genotypic differences sufficiently repeatable in years.The cultivars giving the best yield under stress associated with low susceptibility indices were in bread wheat: Etruria, Spada, Pandas, Centauro, Oderzo, Costantino and Gladio, in durum wheat: Aldura, Arcangelo, Adamello, Vespro and Capeiti, in barley: Fleuret, Barberousse, Jaidor, Express, Trebbia, Georgie, Dahlia, Criter and Magie.     

生物质炭对土壤团聚体微域环境及重金属污染的作用研究

生物质炭作为一种优质的土壤改良剂,由于其实现了资源的可循环利用且在农业及生态领域中有着巨大的应用价值和现实意义,因而近年来受到国内外学者的普遍关注。笔者综述了生物质炭在土壤重金属污染方面的修复作用,并从生物质炭对土壤团聚体微域环境的阳离子交换量、养分及微生物等方面的改善及影响入手,探索了生物质炭对土壤重金属污染的修复机制,研究结果表明生物质炭对土壤重金属形态及生物有效性有显著的固定及改善作用。旨在为该领域未来的研究提出展望和建议,以期为生物质炭的发展动向提供参考。     

Response of wheat cultivars to infection by Stagonospora nodorum isolates/mixture on detached and intact seedling leaves

Stagonospora nodorum blotch (SNB) of wheat caused by Stagonospora nodorum (Berk.) Castellani & E.G. Germano is among the major foliar diseases of wheat worldwide. The response of four wheat cultivars for reaction to infection by isolates/mixture of S. nodorum was tested on detached and intact seedling leaves in the laboratory and glasshouse experiments, respectively. The four wheat cultivars tested significantly differed from each other in response to infection by the isolates/mixture of S. nodorum. Similarly, the aggressiveness of the four isolates/mixture of the pathogen on the four wheat cultivars was significantly different as demonstrated by the parameters tested in the two experiments. However, no significant (p ≤ 0.05) isolate x cultivar interaction was observed for all the parameters tested in the two experiments. Highly significant positive or negative correlations were observed between parameters, i.e., disease severity (DS), incubation period(IP), and size of necrotic lesions (SNL), tested on detached and intact seedling leaves, respectively. This suggests that use of the detached leaf technique has considerable promise for quick screening of wheat cultivars against SNB. This revised version was published online in August 2006 with corrections to the Cover Date.     

Physiological responses to water stress and yield of winter wheat cultivars differing in drought tolerance

Moderate to severe drought (water stress) at the reproductive stage is common in the U.S. Southern High Plains (SHP), where wheat is grown as a major winter crop. The objective of this study was to better understand the physiological basis of drought tolerance in elite wheat cultivars. A 2‐yr glasshouse study was conducted using three cultivars (TAM 111, TAM 112 and TAM 304) and two water treatments (wet: adequate water and dry: water‐limited). Overall, TAM 111 and TAM 112 used more water for cumulative evapotranspiration (ET) and had more tillers and greater root mass and shoot mass compared to TAM 304. In the dry treatment, TAM 112 had 67% and 81% more grain yield than TAM 111 and TAM 304, respectively. Water use efficiency for grain (WUEg) and water use efficiency for biomass (WUEbm) were also greater in TAM 112 compared to the other cultivars in the dry treatment. The flag leaves in TAM 112 at mid‐grain filling stage (about 15 days after flowering) had lower stomatal conductance (Gs), intercellular CO₂ concentration (Ci), transpiration rate (Tr) and net photosynthetic rate (Pn), but higher photosynthetic water use efficiency (PWUE) than TAM 111 and TAM 304 under water stress. This study demonstrated a distinct role of gas exchange parameters in response to drought, and TAM 112 was more efficient than TAM 111 and TAM 304 in evolving physiological mechanisms to adapt to water stress.     

Yield responses by modern wheat genotypes to phosphate fertilizer and their implications for breeding

Summary The response to phosphate fertilizer by modern wheat genotypes was examined in the field under natural rainfall in three seasons. Models were developed which show that grain yield was positively correlated with biological yield and harvest index. In one of the seasons, which was relatively dry, shorter statured wheats gave higher yields at each level of applied phosphate. Higher levels of phosphate tended to offset the reduction in yield associated with late heading and the importance of biological yield on grain yield. The genotypes which produced the largest number of grains m^-2 produced the highest yields.Implications for plant breeding programs are discussed.     

植物修复重金属汞、镉、铬污染土壤的研究进展

土壤重金属污染已经成为了全球研究的热点。植物修复以其简便、经济、无二次污染等优点成为了众多修复手段中的佼佼者。本文归纳了汞、镉、铬污染土壤的单纯植物修复、添加化学调控剂的植物修复以及植物与微生物的联合修复的最新进展,探讨了植物修复土壤重金属存在的问题,并对其发展趋势进行了展望。     

转双价基因棉花对根际土壤酶活性和养分含量的影响

在田间试验条件下,以3种转双价基因棉和常规棉石远321为研究对象,比较分析转双价基因棉和常规棉石远321根际土壤酶活性及养分的变化。结果表明,转双价Cry1Ac+CpTI基因棉sGK321与石远321根际土壤速效磷和铵态氮含量无显著差异,而硝态氮含量则显著高于石远321;转双价Cry1Ac+Cry2Ab基因棉(双Bt抗虫棉)速效磷和铵态氮含量均显著低于石远321,而硝态氮含量与石远321无显著差异;转双价Cry1Ac+Epsps基因棉(抗虫抗除草剂棉)速效磷和硝态氮含量均显著高于石远321,而铵态氮含量显著低于石远321。sGK321棉与石远321根际土壤脲酶、碱性磷酸酶和过氧化氢酶活性均无显著差异;双Bt抗虫棉土壤脲酶活性显著低于石远321,碱性磷酸酶和过氧化氢酶活性与石远321均无显著差异;抗虫抗除草剂棉与石远321土壤脲酶活性无显著差异,碱性磷酸酶活性显著高于石远321,而过氧化氢酶活性显著低于石远321。表明sGK321棉与石远321根际土壤养分(硝态氮除外)含量和酶活性无显著差异,而双Bt抗虫棉和抗虫抗除草剂棉所呈现的差异是因不同品种所致。     

Stem and head reaction of winter wheat cultivars to artificial inoculation by Microdochium nivale under controlled environment and field conditions

Summary Experiments to assess variation in the resistance of winter wheat to infection by Microdochium nivale were conducted over two consecutive years. Resistance was evaluated using an agar disk technique to reproduce stem lesions and by spraying a conidial suspension to reproduce head blight symptoms. Significant variation for stem reaction measured as stem lesion area (SLA), and head reaction measured as disease severity (DS) was found in the 33 winter wheat genotypes tested. Data obtained over two years in controlled environment conditions were significantly correlated (r=0.713 for SLA and r=0.738 for DS), whereas field data showed a significant genotype x year interaction for disease severity. Quantitative variation for susceptibility to stem and head infection by M. nivale was found among the 33 genotypes tested. The majority of genotypes expressed moderate susceptibility, with cultivar Goupil being very susceptible to both stem and head infection, and the remainder, Renan, Arminda, Munstertaler and Saint-Johann were the most resistant. Resistance to stem and head to M. nivale were not correlated (r=0.358).     

Growth and yield responses of spring wheat to increasing carbon dioxide, ozone and physiological stresses: a statistical analysis of ‘ESPACE-wheat’ results

One of the major goals of the European Stress Physiology and Climate Experiment (ESPACE-wheat) was to investigate the sensitivity of wheat growth and productivity to the combined effects of changes in CO₂ concentration, ozone and other physiological stresses. Experiments were performed at different sites throughout Europe, over three consecutive growing-seasons using open-top chambers. This paper summarizes the main experimental findings of the effects of CO₂ enrichment and other factors i.e. ozone (O₃), drought stress or nitrogen supply on the biomass and yield of spring wheat (Triticum aestivum cv. Minaret). Final harvest data from different sites and seasons were statistically analysed: (1) to identify main effects and interactions between experimentally controlled factors; and (2) to evaluate quantitative relationships between environmental variables and biological responses. Generally, ‘Minaret’ wheat did not respond significantly to O₃, suggesting that this cultivar is relatively tolerant to the O₃ levels applied. The main effect of CO₂ was a significant enhancement of grain yield and above-ground biomass in almost all experiments. Significant interactions between CO₂ and other factors were not common, although modifications in different N- and water supplies also led to significant effects on grain yield and biomass. In addition, climatic factors (in particular: mean air temperature and global radiation) were identified as important co-variables affecting grain yield or biomass, repectively. On average, the yield increase as a result of a doubling of [CO₂] was 35% compared with that observed at ambient CO₂ concentrations. However, linear regressions of grain yield or above-ground biomass for individual experiments revealed a large variability in the quantitative responses of ‘Minaret’ wheat to CO₂ enrichment (yield increase ranging from 11 to 121%). Hence, CO₂ responsiveness was shown to differ considerably when the same cultivar of wheat was grown at different European locations. Multiple regression analyses perfomed to evaluate the relative importance of the measured environmental parameters on grain yield indicated that although yield was significantly related to five independent variables (24 h mean CO₂ concentration, 12 h mean O₃ concentration, temperature, radiation, and drought stress), a large proportion of the observed variability remained unexplained.     

Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars

Summary A set of 105 European wheat cultivars, comprising 68 cultivars with known seedling resistance genes and 37 cultivars that had not been tested previously, was tested for resistance to selected Australian pathotypes of P. triticina in seedling greenhouse tests and adult plant field tests. Only 4% of the cultivars were susceptible at all growth stages. Twelve cultivars lacked detectable seedling resistance to leaf rust, and among the remaining cultivars, 10 designated genes were present either singly or in combination. Lr13 was the most frequently detected gene, present in 67 cultivars, followed by the rye-derived gene Lr26, present in 19 cultivars. Other genes present were Lr1, Lr3a, Lr3ka, Lr10, Lr14a, Lr17b, Lr20 and Lr37. There was evidence for unidentified seedling resistance in addition to known resistance genes in 11 cultivars. Field tests with known pathotypes of P. triticina demonstrated that 57% of the cultivars carried adult plant resistance (APR) to P. triticina. The genetic identity of the APR is largely unknown. Genetic studies on selected cultivars with unidentified seedling resistances as well as all of those identified to carry APR are required to determine the number and inheritance of the genes involved, to determine their relationships with previously designated rust resistance genes, and to assess their potential value in breeding for resistance to leaf rust.