广东海风藤超临界流体萃取物的气相色谱-质谱联用分析

目的 分析广东海风藤超临界萃取物的化学成分.方法 采用CO2超临界流体萃取广东海风藤的脂溶性部位,以气相色谱-质谱联用(GC-MS)法鉴定化学成分,面积归一化法测定各成分相对含量.结果 从广东海风藤超临界萃取物中共分离出28个组分,鉴定了其中25个化合物,占萃取物总量的78.33%,主要为T-杜松醇(18.195%)、8-杜松烯(11.485%)、α-没药醇(8.304%)和瓦伦烯(6.672%).结论 广东海风藤的挥发油提取物中主要含有萜烯和萜醇.     

广东海风藤的研究概况

分析国内外对广东海风藤的研究情况。近年来通过药学工作者不懈的努力,广东海风藤在原植物鉴定和本草考证、化学成分、药理作用、临床应用等方面的研究都有了很大进展。本文所介绍的广东海风藤药材为木兰科南五味子属植物海风藤的干燥藤茎,有别于《中国药典》收载的海风藤植物。广东海风藤的化学成分主要是木脂素和萜类,均为其有效成分,分别有抗脂质的过氧化作用和抑制胆固醇生物合成作用。广东海风藤作为中药在临床上应用广泛,主要是治疗风湿骨痛和气管炎。本文分别就这些方面作一概述。     

广东海风藤中木脂素和五味子乙素的含量测定

目的：对广东海风藤药材超临界二氧化碳萃取物中木脂素的含量进行测定。方法：采用超临界二氧化碳萃取广东海风藤药材，萃取压力10MPa，萃取温度45℃，分离压力5MPa，分离温度50℃。采用比色法和HPLC法分别测定了萃取物中总木脂素和五味子乙素的含量。结果：广东海风藤药材超临界二氧化碳萃取物中总木脂素平均含量以五味子酯甲计为96．6mg·g^-1，RSD为2．77％；五味子乙素平均含量为25．53mg·g^-1，RSD为2．71％。结论：采用超临界二氧化碳萃取法能有效地提取出广东海风藤中木脂素成分。本实验所采用方法可作为广东海风藤药材及其制剂的质量控制方法。     

广东海风藤的研究概况

分析国内外对广东海风藤的研究情况.近年来通过药学工作者不懈的努力,广东海风藤在原植物鉴定和本草考证、化学成分、药理作用、临床应用等方面的研究都有了很大进展.本文所介绍的广东海风藤药材为木兰科南五味子属植物海风藤的干燥藤茎,有别于<中国药典>收载的海风藤植物.广东海风藤的化学成分主要是木脂素和萜类,均为其有效成分,分别有抗脂质的过氧化作用和抑制胆固醇生物合成作用.广东海风藤作为中药在临床上应用广泛,主要是治疗风湿骨痛和气管炎.本文分别就这些方面作一概述.     

不同产地木香挥发油成分的GC-MS分析比较

目的:比较不同产地木香药材中挥发油的含量和化学成分的差异。方法:采用水蒸气蒸馏法提取7批不同产地木香的挥发油,用气相色谱-质谱(GC-MS)联用法对挥发油进行化学成分的分析鉴定,用面积归一化法获得各化合物的相对百分含量。结果:药材中挥发油含量分别为0.8%～2.1%,从挥发油中共鉴定了46种化学成分,不同产地木香挥发油的主要成分组成均为内酯类、萜类化合物,但相对含量存在一定的差异。结论:用该方法能为木香药材的质量鉴别提供较好的依据。     

广东产中药桂枝挥发油成分分析

目的：分析广东产中药桂枝挥发油中的化学成分。方法：采用分辨毛细管气相色谱－质谱（HRCGC－MS）联用技术分析广东产桂枝挥发没成分。结果：鉴定出49个成分，含量在1％以上有12个，以桂皮醛（83％）含量最高。已鉴定的成分占挥发油总量的985，结论：为广东建立中药材桂枝的GAP生产基地提供了科学依据。     

黑老虎药材挥发油成分超临界CO2萃取与水蒸汽蒸馏工艺比较

目的 研究黑老虎药材用2种不同提取方法所得挥发油成分及收率.方法 用超临界CO2萃取技术及水蒸汽蒸馏法从黑老虎药材中分离出挥发油,并用气相色谱/质谱联用技术测定其化学成分.结果 从2种不同方法所得挥发油中分别鉴定出27种和25种成分.其中β-石竹烯、δ-杜松烯、法尼醇、芹子烯为黑老虎药材挥发油的主要成分.结论 超临界C...     

不同产地莪术挥发油气相色谱-质谱联用分析

目的：对三种不同产地温莪术、蓬莪术以及广西莪术挥发油的化学成分进行了GC—MS分析比较。方法：采用水蒸气蒸馏法提取三种不同产地的莪术挥发油,并通过气相色谱-质谱联用技术,对其中的化学成分进行分析鉴定．．结果：初步鉴定了三种不同产地莪术挥发油中的主要成分,不同产地莪术挥发油成分有一定差异。结论：为莪术药材的质量评价提供依据。     

黑老虎药材挥发油成分超临界CO_2萃取与水蒸汽蒸馏工艺比较

目的研究黑老虎药材用2种不同提取方法所得挥发油成分及收率。方法用超临界CO2萃取技术及水蒸汽蒸馏法从黑老虎药材中分离出挥发油,并用气相色谱/质谱联用技术测定其化学成分。结果从2种不同方法所得挥发油中分别鉴定出27种和25种成分。其中β-石竹烯、δ-杜松烯、法尼醇、芹子烯为黑老虎药材挥发油的主要成分。结论超临界CO2萃取法比水蒸汽蒸馏法从黑老虎中分离的挥发油收率高,且工艺耗时少。     

气相色谱-质谱分析牛至药材“地下部分”所得挥发油的化学成分

目的:研究牛至药材"地下部分"所得挥发油的化学成分。方法:采用水蒸气蒸馏法提取牛至药材"地下部分"(根)的挥发油,并用GC-MS法对其化学成分进行分析与鉴定,通过峰面积归一化法计算出各成分的相对百分含量。结果:牛至药材"地下部分"(根)挥发油中鉴定出29个化合物,占挥发油总量的 98.97%,其中10个为首次报道。结论:牛至药材"地下部位"(根)中除含有"地上部分"同类的抗菌成分外,还含有大量脂肪酸类化合物,该类物质具有较强的抗氧化作用,不能被完全忽略,同样具有一定的药用价值。     

New triterpenoids from Kadsura heteroclita and their cytotoxic activity

Phytochemical investigations of the stem of Kadsura heteroclita (Roxb) Craib (Schizandraceae) resulted in the isolation and structure elucidation of six new triterpenoidal compounds named heteroclitalactones A-E (1-5) as well as heteroclic acid (6) and heteroclitalactone F (7), which was isolated for the first time from a natural source, and the six known compounds schisanlactone E (8), cycloartenone (9), schisandronic acid (10), nigranoic acid (11), changnanic acid (12) and schisanlactone B (13), respectively. The structures of these compounds were characterized by extensive 1D and 2D NMR spectral analyses. The majority of these triterpenoids showed moderate cytotoxic activity against the human tumor cell lines Bel-7402, BGC-823, MCF-7 and HL-60. Among the compounds tested, heteroclitalactone D (4) showed the strongest cytotoxic activity against the HL-60 cells with an IC50 of 6.76 microM.     

Simultaneously qualitative and quantitative analysis of the representative components in Kadsura heteroclita stem by UHPLC-Q-Orbitrap HRMS

Kadsura heteroclita (Roxb) Craib stem (KHS) is a medicinal plant used for the treatment of rheumatism arthritis diseases in Tujia ethnomedicine. Thus far, the complex chemical compositions in KHS are not clear, and the levels of the major compounds in KHS are not well understood. In this study, a novel UHPLC-Q-Orbitrap HRMS method was established for the simultaneous quali-quantitative analysis of KHS. A total of 204 compounds were identified, including triterpenoids, lignans, sesquiterpenes, fatty acids, phenolic acids, and flavonoids, more than 100 of which were first discovered in KHS. Using the same method, 12 representative bioactive components were successfully quantified. The method was fully validated by linearity, LOD, LOQ, precision, stability, recovery, and matrix effects, and it was applied to quantify the 12 representative compounds in 4 batches of KHS. As this method enables retrospective data analysis and has no upper limit to the number of analytes in a single run, it can be applied to quantify more active components of KHS in the future.     

异型南五昧子(Kadsura heteroclita)中的具有血小板活化因子(PAF)拮抗活性的联苯环辛烯类木脂素

自异型南五味子(Kadsure heteroclita)首次分得7个已知的联苯环辛烯类木脂素,tigloylgomisin P(1),angeloylgomisin P(2),gomisin A(3),五味子素(4),angeloylgomisin H(5),gomisin B(6),R(+)-gomisin M(7)。tigloylgomisin P(1)的构型以二维核磁共振光谱技术确定。应用[~3H]血小板活化因子(PAF)结合人血小板膜的分析方法测定,证明化合物(1),(2)和(7)有 PAF 受体拮抗活性。     

Four new lignans from the stems of Kadsura heteroclita

Two new C(18) dibenzocyclooctadiene lignans named heteroclitalignans A and B (1 and 3), one new C(19) homolignan named heteroclitalignan C(4) and one new natural product named heteroclitalignan D(2), together with the four known lignans kadsurarin (5), kadsulignan E (6), d-epigalbacin, and meso-dihydroguaiaretic acid were isolated from the stems of Kadsura heteroclita. The structures of these compounds were characterized by extensive 1D and 2D NMR spectral data analysis.     

Antifungal Activity of the Essential Oils of Nine Zingiberaceae Species

The rhizome oils of nine Zingiberaceae species [Zingiber officinale Rosc., Z. cassumunar Roxb., Z. zerumbet Smith, Curcuma aeruginosa Roxb., C. mangga Valeton and van Zyp, C. xanthorrhiza Roxb., Kaempferia galanga Linn., Alpinia galanga Swartz and Boesenbergia pandurata (Roxb.) Schlecht] were investigated for their antifungal activities against five dermatophytes (Trichophyton mentagrophytes, T. rubrum, Microsporum canis, M. nanum and Epidermophyton floccosum), three filamentous fungi (Aspergillus niger, A. fumigatus and Mucor sp.) and five strains of yeast (Saccharomyces cerevisiae, Cryptococcus neoformans, Candida albicans, Ca. tropicalis and Torulopsis glabrata). The antifungal testing was carried out by using the broth microdilution and the disc gel diffusion methods. Amongst the essential oils studied, only the oil of B. pandurata was effective against all the fungi, exhibiting the lowest MIC values of 0.63µgµl -1 to Mucor sp., 1.25µgµl -1 to both A. niger and A. fumigatus, and 2.5µgµl -1 to both T. rubrum and E. floccosum, and the highest inhibition zone diameter of 20.6mm against S. cerevisiae. The essential oil of K. galanga showed selective toxicity against A. fumigatus with a MIC value of 0.63µgµl -1, while the essential oils of Z. officinale and Z. cassumunar exhibited high activity against the yeasts (11.7-15.7mm). The chemical composition of the active essential oils was investigated by GC and GC-MS.     

我国姜科药用植物研究——Ⅳ.姜三七醌(Stahlianthusone)的化学结构

从草药姜三七的挥发油得到一种新倍半萜醌,分子式C_(15)H_(16)O_2,定名为姜三七醌(Ia)(Stahlianthusone)。经光谱分析和化学反应测定了其化学结构为1,6-二甲基4-异丙基萘醌-5,8。     

我国姜科药用植物研究——Ⅳ.姜三七醌(Stahlianthusone)的化学结构

从草药姜三七的挥发油得到一种新倍半萜醌,分子式C₁₅H₁₆O₂,定名为姜三七醌(Ia)(Stahlianthusone)。经光谱分析和化学反应测定了其化学结构为1,6-二甲基4-异丙基萘醌-5,8。     

Chemical composition,antibacterial and antifungal activities of essential oil from <Emphasis Type="Italic">Heracleum Siamicum Craib</Emphasis>

Heracleum siamicum Craib (Apiaceae), is an important herbal species having wide application in food flavoring processes. The flat-oval shaped fruit of H. siamicum Craib from North Thailand was hydrodistilled, and the chemical composition of the essential oil was analyzed by GC and GC-MS. The essential oil yield based on dried plant material was 1.25%, and twenty-five compounds (corresponding to 97.69% of the total weight) were identified. The main components were: n-octyl acetate (65.30%), o-cymene (10.35%), limonene (7.52%), δ-2-carene (6.87%), cis-thujone (1.92%), isobornyl acetate (0.94%), n-octanol (0.73%), 1,8-cineol (0.62%), n-tridecanol (0.44%), and safrole (0.37%). H. siamicum essential oil demonstrated bactericidal and fungicidal activity against five bacterial strains and two fungal strains, as evaluated using agar diffusion in terms of the minimum inhibitory concentration.