Clinical Observation on Effect of Yangjing Decoction in Promoting Follicular Growth

Clinical Observation on Effect of Yangjing Decoction in Promoting Follicular Growth@陆华@刘敏如@李春梅     

Clinical Study on Protective Effect of Ginaton on Ischemia-Reperfusion Injured Myocardium during Cardiopulmonary Bypass

Ｇｉｎａｔｏｎ（Ｇｉｎｋｇｏｂｉｌｏｂａｅｘｔｒａｃｔ，ＧＢＥ）ｉｓａｖａｓｏｄｉｌａｔｏｒｏｆｃｅｒｅｂｒａｌａｎｄｃａｒｄｉａｌｖｅｓｓｅｌｓ，ｗｈｉｃｈｃａｎｓｃａｖｅｎｇｅｏｘｙｇｅｎｆｒｅｅｒａｄｉｃａｌｓ，ｓｐｅｃｉｆｉｃａｎｔａｇ ｏｎｉｚｉ     

Study on Influence of Sini Decoction (四逆汤) on Quality of Life of Patients after Percutane ous Transluminal Coronary Angioplasty

Ｐｅｒｃｕｔａｎｅｏｕｓｔｒａｎｓｌｕｍｉｎａｌｃｏｒｏｎａｒｙａｎｇｉｏｐｌａｓｔｙ（ＰＴＣＡ）ｉｓｏｎｅｏｆｔｈｅｍａｉｎｍｅｔｈｏｄｓｆｏｒｒｅｌｉｅｖｉｎｇｍｙｏｃａｒｄｉａｌｉｓｃｈｅｍｉａｉｎｃｏｒｏｎａｒｙｈｅａｒｔｄｉｓｅａｓｅｃｌｉｎｉｃａｌｌｙ．ＴｈｅｌａｒｇｅａｍｏｕｎｔｏｆｔｏｘｉｃｆｒｅｅｒａｄｉｃａｌｓｐｒｏｄｕｃｅｄｂｙＰＴＣＡｃｏｕｌｄｉｎｄｕｃｅｈａｒｍｆｕｌｌｉｐｉｄｐｅｒｏｘｉｄａｔｉｏｎ（ＬＰＯ）．Ｔｈｅａｕｔｈｏｒｓ＇ｐｒｅｌｉｍｉｎａｒｙｓｔｕｄｙｈａ…     

Antioxidative Effects of Rubidate on Ischemic Myocardium in Mice

ＡｎｔｉｏｘｉｄａｔｉｖｅＥｆｆｅｃｔｓｏｆＲｕｂｉｄａｔｅｏｎｌｓｃｈｅｍｉｃＭｙｏｃａｒｄｉｕｍｉｎＭｉｃｅＡｎｔｉｏｘｉｄａｔｉｖｅＥｆｆｅｃｔｓｏｆＲｕｂｉｄａｔｅｏｎｌｓｃｈｅｍｉｃＭｙｏｃａｒｄｉｕｍｉｎＭｉｃｅＹＡＮＧＳｈｅｎｇ－ｌｉ（...     

Protective Effect of Salvia Miltiorrhizae Composita on Myocardium in Patients Undergoing Open Heart Surgery

Protective Effect of Salvia Miltiorrhizae Composita on Myocardium in Patients Undergoing Open Heart Surgery@顾家珍@李俊@刘世清@张红艳@余金甫@夏正远     

Protective Effect of Zengye Decoction(增液汤)on Submandibular Glands in Nonobese Diabetic Mice

Objective: To investigate the protective effect of Zengye Decoction(增液汤, ZYD) on the submandibular glands(SMGs) in nonobese diabetic(NOD) mice. Methods: Twenty-seven female NOD mice were randomly equally divided into 3 groups: the model group, the hydroxychloroquine(HCQ) group, and the ZYD group. Nine C57/B6 mice served as the normal group. After 1-week acclimation, the HCQ and ZYD groups were intragastrically administered with HCQ and ZYD, respectively, and the normal and model groups were administered with normal saline. Changes in the salivary flow rate were observed. Mice from all 4 groups were sacrificed at the age of 20 weeks. The serum and SMGs were collected. Serum cytokines gamma-interferon(IFN-γ), interleukin-10(IL-10) were detected by enzyme-linked immunosorbent assay. Histological changes in the submandibular glands were examined by hematoxylin and eosin staining. The mRNA expression of IFN-γ, IL-10 and vasoactive intestinal peptide(VIP) in the submandibular glands were measured by realtime polymerase chain reaction. Results: Compared with the model group, the salivary flow of the ZYD group significantly increased(P0.05), the extent of the histological changes was ameliorated(P0.05), and the Th1/Th2 cytokine imbalance was remedied(P0.05). In the ZYD-treated mice, the VIP mRNA was up-regulated(P0.05). Conclusions: ZYD is beneficial in protecting structure and function of SMGs in NOD mice. The mechanism may be associated with the correction of the Th1/Th2 cytokine imbalance, and with the prevention of a progressive decline of the VIP level.     

A Preliminary Observation on Effect of Qigong（气功）on Electrocardiographic Autopower Spectrum Funotion

ＡＰｒｅｌｉｍｉｎａｒｙＯｂｓｅｒｖａｔｉｏｎｏｎＥｆｆｅｃｔｏｔＱｉｇｏｎｇ（气功）ｏｎＥｌｅｃｔｒｏｃａｒｄｉｏｇｒａｐｈｉｃＡｕｔｏｐｏｗｅｒＳｐｅｃｔｒｕｍＦｕｎｏｔｉｏｎＪＩＮＫｅ－ｑｉ（金科奇）ａｎｄＣＨＥＮＷａｎ－ｃｈｕｎ（陈万春）（Ｓ...     

Protective Effect of Radix Ginseng on Peroxidation Injury in Myocardium and Erythrocytes of Streptozocin－Induced Diabetic Rats

ＰｒｏｔｅｃｔｉｖｅＥｆｆｅｃｔｏｆＲａｄｉｘＧｉｎｓｅｎｇｏｎＰｅｒｏｘｉｄａｔｉｏｎＩｎｊｕｒｙｉｎＭｙｏｃａｒｄｉｕｍａｎｄＥｒｙｔｈｒｏｃｙｔｅｓｏｆＳｔｒｅｐｔｏｚｏｃｉｎ－ＩｎｄｕｃｅｄＤｉａｂｅｔｉｃＲａｔｓＸｉｅＺｏｎｇ－ｃｈａｎｇ（...     

Effect of Xiaobailing Decoction on Melanocytes in Vitro

Objective: To observe the effect of Xiaobailing Decoction(消白灵汤)on murine melanocytes in vitro and to explore the mechanism of Xiaobailing Decoction in the treatment of vitiligo. Methods: B-16F10 murine melanoma cells were cultured in 1640 medium and treated respectively with different concentrations (1mg/ml, 2mg/ml, 3mg/ml) of the Chinese drug Xiaobailing Decoction and its main components, the drugs for replenishing the kidney-yang, and the drugs for nourishing blood and activating blood circulation, etc. for 7 days. MMT assay was used to determine the proliferation of B-16F10 murine melanoma cells. NaOH cleavage assay was adopted to measure the melanogenesis of melanocytes. Results: Xiaobailing Decoction, the drugs for replenishing the kidney-yang and the drugs for nourishing blood and activating blood circulation at different concentrations significantly improved the proliferation of B-16F10 murine melanoma cells from the 3rd day to the 5th day (P<0.05), with Xiaobailing Decoction at the concentrations of 1mg/ml having the most distinct action on promoting the proliferation of melanocytes on the 3rd day (P<0.001); And the drugs for replenishing the kidney-yang at the concentrations of 2mg/ml and 3mg/ml and the drugs for nourishing blood and activating blood circulation at 3mg/ml significantly increased melanogenesis of melanocytes (P<0.05). Conclusion: Xiaobailing Decoction can promote melanocytic proliferation and melanogenesis in vitro, and it is indicated that the drugs for replenishing the kidney-yang and the drugs for nourishing blood and activating blood circulation play an important role in treating vitiligo.     

The Effect of Hypercapnic Acidosis Preconditioning on Rabbit Myocardium

This study observed the protective effect of hypercapnic acidosis preconditioning on rabbit heart suffered from ischemia-reperfusion injury. Hypercapnic acidosis was established in animals with mechanical hypoventilation before ischemia-reperfusion. Thirty-two rabbits were randomly divided into 4 groups, with each having 8 aminals in term of the degree of acidification： hypercapnic acidosis group A （group A）, hypercapnic acidosis group B （group B）, hypercapnic acidosis group C （group C）, ischemia and reperfusion group （group IR）. Animals in group IR were ventilated normally （tidal volume： 15 mL/kg, breathing rate 35 bpm）. The PETCO2 was maintained at the level of 40-50 mmHg for 30 min. Animals in groups A, B, C received low-frequency, low-volume ventilation to achieve hypercarbonic acidosis and the target levels of PETCO2 were 75-85 ,65-75, 55-65 mmHg, respectively, with levels being maintained for 5 min. The animals then were ventilated normally to lower PETCO2 to 40-50 mmHg. The left anterior branch artery of all the animals was ligated for 30 min and reperfused for 180 min. Then the infarct size was calculated. The cardiomyocytes were morphologically observed and ECG and hemodynamics were monitored on continuous basis. Acid-base balance was measured during procedure. Our results showed that the infarct size was （48.5±11.5）% of the risk area in the control group and （42.4±7.9）% in group C （P〉0.05）. Mean infarct size was significantly smaller in group B （34.5%±9.4%） （P〈0.05 vs control group） and group A （31.0%±9.1%） （P〈0.01 vs control group）. It is concluded that HA-preconditioning can effectively protect the myocardium.     

Protective Effects of Hyperpolarizing Cardioplegia with Pinacidil on Myocardium in Rats

Whether the ATP-sensitive potassium channel opener pinacidil can provide myocardial protective effects in prolonged isolated global ischemic rat heart was investigated. On modified isolated rat working heart model, 40 hearts were divided into four groups randomly: Hyperpolarized arrest H-K solution containing pinacidil (50 tmol/L) (P1 and P2) and depolarized arrest St. Thomas‘ solution (S1 and S2) subjected to 15 C hypothermia, 60 min (P1 and S1) or 120 min (P1 and S2) of ischemia and 30 min reperfusion. The experimental indices included cardioplegic efficiency, cardiac function, coronary blood flow, myocardial enzyme release, myocardial water and ATP content. Hyperpolarized arrest provided significantly better recovery of cardiac function than depolarized arrest.Postischemic coronary flow and myocardial ATP content were higher. The arrest time of electro-mechanical activities were longer than depolarized arrest. There were no differences among the groups in myocardial water contents. The hyperpolarized arrest solution containing pinacidil can provide a marked myocardial protective effect during prolonged hypothermic myocardial ischemia.     

Clinical Observation of Bronchial AsthmaTreeted with Qingre Dingchuan Decoction（清热定喘汤）

ＣｌｉｎｉｃａｌＯｂｓｅｒｖａｔｉｏｎｏｆＢｒｏｎｃｈｉａｌＡｓｔｈｍａＴｒｅｅｔｅｄｗｉｔｈＱｉｎｇｒｅＤｉｎｇｃｈｕａｎＤｅｃｏｃｔｉｏｎ（清热定喘汤）ＣｈｕＤｏｎｇ－ｎｉｎｇ（褚东宁）（Ｐｅｏｐｌｅ＇ｓＨｏｓｐｉｔａｌｏｆＳｈａｎｇｃｈｅｎｇＤ...     

Observation on Effect of Yi'an Decoction(胰安汤) in Treating 65 Patients of Acute Pancreatitis

Ａｃｕｔｅｐａｎｃｒｅａｔｉｔｉｓ（ＡＰ）ｏｎｓｅｔｓｓｕｄｄｅｎｌｙ,ａｎｄｓｅｒｉｏｕｓｌｙ．Ｃｕｒｒｅｎｔｖｉｅｗｔｅｎｄｓｔｏｔｒｅａｔｉｔｎｏｎｏｐｅｒａｔｉｖｅｌｙ．Ｓｉｎｃｅ 1 994,ｔｈｅａｕｔｈｏｒｓｈａｖｅｔｒｅａｔｅｄ 1 0 0ＡＰ ｐａｔ     

The Protective Effect of L-carnitine on Ischemia-reperfusion Heart

L-carnitine is crucial to the metabolismof fat-ty acids and essential in transportation of long-chain fatty acids fromcytosol intothe mitochondri-a , whereβ-oxidation takes place and biological en-ergyin the formof ATP can be synthesized.Past studies showed that L-carnitine can pro-tect the ischemia-reperfusion injured heart by re-ducing infarct size and apoptotic cell death rate ,decreasingincidence of ventricular fibrillation,andi mproving mechanical function[1].Losteret alreportedthat L-…     

Effect of TheoPolyphenols(茶多酚)on Function of TransMembranous Signal System of Acute Ischemic Myocardium in Rats

ｔｉｓｔｈｅｍｙｏｃａｒｄｉａｌｉｓｃｈｅｍｉａａｎｄｉｓｃｈｅｍｉｃｒｅｐｅｒｆｕｓｉｏｎｉｎｊｕｒｙｔｈａｔｃｏｎｔｅｍｐｏｒａｒｙｓｔｕｄｙｆｏｃｕｓｓｅｄｕｐｏｎ．Ｉｔｈａｓｂｅｅｎｐｒｏｖｅｄｔｈａｔｔｈｅｏｐｏｌｙｐｈｅｎｏｌｓ（ＴＰ）ｈａｓｅｆｆｅｃｔｏｆａｎｔｉｏｘｙｇｅｎ,ｓｃａｖｅｎｇｉｎｇｏｘｙｇｅｎｆｒｅｅｒａｄｉｃａｌ,ａｄｊｕｓｔｉｎｇｓｅｒｕｍｌｉｐｉｄｓ,ｅｔｃ（１,２）．Ｂｕｔｉｔｉｓｕｎｃｌｅａｒｗｈａｔｉｓｉｔｓｍｏｌｅｃｕｌａｒｂｉｏｌｏｇｉｃａ…     

Intervention Effect of Jianxin Decoction（健心汤） on Serum Cytokine Level of Congestive Heart Failure Patients

Objective: To investigate the intervention effect of Jianxin Decoction (健心汤, JXD) on the cytokine level in serum of patients with congestive heart failure (CHF). Methods: Sixty-six patients with CHF were randomly divided into the control group (n=33) and the trial group (n=33). The control group received conventional treatment, and the trial group was treated with conventional therapy plus JXD for 4weeks. Before and after treatment, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and nitrogen monoxide (NO) in serum and cardiac function were determined. Results: After treatment, the levels of TNF-α,IL-6 and NO were significantly lower than those before treatment (P＜0.05, or P＜0. 01) in the two groups,and the lowering degree of the indices in the trial group was more significantly reduced than that in the control group (P＜0. 05). And cardiac functions in both groups were improved significantly (P ＜ 0.05, or P ＜0.01). Conclusion: JXD could prevent and reverse ventricular remodeling so as to ameliorate cardiac function through regulating the levels of cytokines.     

Clinical Observation on Treatment of Ischemic Apoplexy by Electroacupuncture plus Acupoint-Injection

Objective: To observe the therapeutic effects of electroacupuncture plus acupoint-injection for ischemic apoplexy. Method: 78 cases of ischemic apoplexy were randomly divided into a treatment group of 39 cases treated by electroacupuncture plus acupoint-injection and a control group of 39 cases treated by electroacupuncture alone. Results: The neural functional defect score (NFDS) decreased while the functional independence mark (FIM) increased with obvious significant differences before and after treatment in both of the two groups. And there were very remarkable differences between the two groups in the changes of both NFDS and FIM after treatment (P<0.01), showing better results in the treatment group than that of the control group. Conclusion: Electroacupuncture plus acupoint-injection can promote the recovery of the neural function for the ischemic apoplexy patients and markedly enhance their daily living ability, with better effects than that in patients treated by electroacupuncture alone.     

The Protective Effect of Colostrum on Neonates Intestine.

The author determined the concentration of secretory immunogiobulin A (SIgA) in the colostrum and the feces of 15 neonates during the first 5 days after birth and also, the bacterial count as well as the frequency of bifidobacteria ap-     

Protective Effects of Traditional Tibetan Medicine Zuo-Mu-A Decoction (佐木阿汤) on the Blood Parameters and Myocardium of High Altitude Polycythemia Model Rats

Objective: To explore the protective effects of Tibetan medicine Zuo-Mu-A Decoction(佐木阿汤, ZMAD) on the blood parameters and myocardium of high altitude polycythemia(HAPC) model rats. Methods: Forty male Wistar rats were randomly divided into 4 groups by a random number table, including the normal, model, Rhodiola rosea L.(RRL) and ZMAD groups(10 in each group). Every group was raised in Lhasa to create a HAPC model except the normal group. After modeling, rats in the RRL and the ZMAD groups were administered intragastrically with RRL(20 mL/kg) and ZMAD(7.5 mL/kg) once a day for 2 months, respectively; for the normal and the model groups, 5 mL of distilled water was administered intragastrically instead of decoction. Then routine blood and hematologic rheology parameters were taken, levels of erythropoietin and 8-hydroxy-2'-deoxyguanosine(8-OHd G) were tested, and ultrastructural change in the left ventricular myocardium was observed using transmission electron microscopy. Results: Compared with the model group, ZMAD significantly reduced the red blood cell count, hemoglobin levels, whole blood viscosity at low/middle shear rates, plasma viscosity, erythrocyte electrophoretic time, erythropoietin and 8-OHd G levels, and also increased the erythrocyte deformation index(P0.05). There was no difference in all results between the RRL and the ZMAD groups. The cardiac muscle fibers were well-protected, mitochondrial matrix swelled mildly and ultrastructure changes were less prominent in the ZMAD group compared with the model group. Conclusion: ZMAD has significant protective effects on the blood parameters against HAPC, and also has the beneficial effect in protecting against myocardial injury.