固定化沼泽红假单胞菌去除Pb2+的研究

对固定化沼泽红假单胞菌与水相中重金属离子Pb^2＋的生物吸附情况进行了研究。主要研究了溶液的pH、不同供氧光照、吸附时间、Pb^2＋初始质量浓度和温度等对生物去除效果的影响。结果表明：在pH为7、厌氧光照、温度35℃、吸附12h和Pb^2＋质量浓度100mg／L条件下,固定化沼泽红假单胞菌对Pb^2＋的去除率可达98%。在实验的浓度范围内固定化沼泽红假单胞菌颗粒对Pb^2＋的吸附符合Langmuir吸附模型和Freundlich吸附模型,并且符合Langmuir吸附模型的程度更优。     

沼泽红假单胞菌处理HMX研究

研究了驯化后的沼泽红假单胞菌(rhodopseudomonas palustris)生长细胞在不同条件下对奥克托今(HMX)的降解效果。结果表明,沼泽红假单胞菌具有较高的细胞活性,并对HMX具有较强的降解能力。在HMX质量浓度为130mg/L时,该菌株生长细胞降解的最佳条件为:温度25℃,pH值7;光照厌氧条件下的降解效率远远高于光照好氧条件下的降解效率,降解效率达到85.7%;HMX作为该菌株唯一的碳源和能源,降解效率与细胞浓度呈极显著相关,细菌降解效率保持在较恒定的水平。     

沼泽红假单胞菌降解TNT废水的研究

本文研究了外加氮源对沼泽红假单胞菌降解TNT的影响,利用4因素3水平L9(43)的正交实验研究了该菌种对TNT废水的降解特性.结果表明:在最佳外加氮源KNO3浓度为0.1g/L时,沼泽红假单胞菌降解TNT的最优组合为:TNT浓度=50mg/L,温度=30℃,pH=7,接种量=108个/ml.     

铝絮凝剂絮凝沼泽红假单胞菌研究

研究了4种不同铝絮凝剂Al2(SO4)3、明矾、AlCl3和聚合氯化铝(PAC)絮凝沉降沼泽红假单胞菌细胞的效果.结果表明,PAC的絮凝性能最好,在0.01 mol·L-1的较低浓度下可以将100%的沼泽红假单胞菌细胞絮凝沉降.进一步研究显示,对于不同铝絮凝剂,在pH值为6～9时,随pH值的降低,沼泽红假单胞菌细胞的絮凝沉降效率逐步提高,其中仍然是PAC的絮凝效果最好.为从培养物中收集沼泽红假单胞菌细胞的产业化生产奠定了重要的基础.     

流化床固定化沼泽红假单胞菌处理废水的研究

沼泽红假单胞菌是研究和应用较为广泛的一种光合细菌,可以净化水质。而采用固定化技术对细胞进行包埋后,既能使废水处理装置内维持较高浓度的生物量,提高处理负荷,又能选择地使光合细菌成为优势菌群,提高水质净化效率和延长细菌使用时间。本实验以海藻酸钙为载体包埋沼泽红假单胞菌,研究了不同浓度的海藻酸钠和氯化钙对固定化小球的机械强度、传质速率等指标的影响,确定适合沼泽红假单胞菌包埋的海藻酸钠和氯化钙浓度分别为：2％-2．5％和5％。另外,本实验还研究了水力停留时间（HRT）、温度、pH值、曝气对模拟废水COD去除率的影响,得出最佳处理条件为：HRT=6h,温度=30-35℃,pH值=7,曝气。     

沼泽红假单胞菌对活性大红废水处理的研究

研究了驯化后的光合细菌N菌株—沼泽红假单胞菌的生长细胞在不同条件下对活性大红的脱色处理效果。结果表明,沼泽红假单胞菌具有较高的细胞活性,并对活性大红具有较强的脱色处理能力。在活性大红浓度为100mg/L时,该菌株生长细胞脱色的最佳条件为:温度30℃,pH值为7,光照厌氧条件下的脱色效率远远高于光照好氧条件下的脱色效率,脱色效率达到80.2%,活性大红作为该菌株唯一的碳源和能源,脱色效率与细胞浓度呈极显著相关,细菌脱色效率保持在较恒定的水平。     

电气石对液态水团簇和沼泽红假单胞菌脱氢酶活性的影响

用化学个分析、X射线衍射分析、原子力显微镜等方法对内蒙古产黑色电气石进行测试表征。研究了电气石对液态水团簇和沼泽红假单胞菌脱氧酶活性的影响。结果显示：电气石使蒸馏水17^O核磁共振的半高幅宽变窄,降低了水分子缔合度。电气石提高了细胞脱氢酶活性,并使脱氧酶对pH值变化的稳定性增强。电气石对体系pH调控的结果表明：电气石使不同起始pH值溶液的pH值均趋向8．0左右。电气石提高脱氧酶活力除了电气石影响水的团簇外,还与其保持体系恒定的弱碱性pH有关。     

红假单胞菌浮选脱硫影响因素研究

采用微生物预处理-浮选联合工艺流程,利用红假单胞菌对高硫煤进行微生物-浮选脱硫实验,重点考察了预处理时间、矿浆浓度、pH值和细菌浓度等因素对浮选脱硫效果的影响．研究结果表明,微生物-浮选脱硫是一种高效的脱硫方法,红假单胞菌是一种有效的浮选脱硫菌种,其预处理时间、体系的pH值、矿浆浓度和细菌浓度等因素对脱硫效果有着显著的影响。     

铜离子吸附假单胞菌MEW079筛选鉴定及其发酵条件优化

为了寻找高效吸附铜离子的菌株,以重金属污水为筛选对象,筛选到一株细菌MEW079.经鉴定,MEW079归属为假单胞菌属(Pseudomonas sp.).以菌株(菌浓度OD600=1.0)对100 mg/L铜离子的吸附效率为指标,通过单因素实验、最陡爬坡路径试验和Box-Behnken响应面实验,优化了MEW079的发...     

两株假单胞菌对烷烃的摄取和降解

根据细菌在油水混合体系中的分布差异,从江苏油田附近污染的土样中筛选得到了两株对烷烃具有降解能力的假单胞菌Pseudomonas sp. A和Pseudomonas sp. B. 在以煤油为唯一碳源的培养基中,Pseudomonas sp. A的生长量较低,菌体主要聚集在油相中,菌体细胞呈亲油性,生长几乎不受生物表面活性剂抑制剂EDTA的影响,菌体的生长对培养基的表面张力的降低幅度较小;而Pseudomonas sp. B则表现相反的性质. 说明两菌株对烷烃具有不同的摄取机制,Pseudomonas sp. A是以直接接触烷烃的方式摄取,而Pseudomonas sp. B则是通过产生生物表面活性剂对烷烃进行乳化后再摄取.     

Removal of sulfide ions from aqueous solutions using carbon- and silicon-containing sorbents

Sulfide ions are among the most common pollutants in natural waters. Sulfide sorption methods are widely used in the waste water treatment practice. The most promising sorbents for removing sulfide ions are porous carbon materials. In the present study we researched sorption capacity of the carbon- and silicon-containing samples of rice and buckwheat processing wastes, as well as the activated carbon, carbon fiber, chitosan and natural silicates toward the sulfide ions in aqueous solutions. It was found that the most effective sorbent from the studied ones is the carbon fiber Aktilen B (99%) and from plant wastes – carbonaceous sorbents obtained from rice straw (77–98%) and buckwheat (94%). We studied the physico-chemical and structural properties of the carbonaceous sorbent based on rice straw and it was found that this sample is amorphous and has a predominant microporous structure. At the concentration of sulfide ions ranging from 140 to 800?µg?l^?1, the studied sorbent reduces the amount of sulfide ions to 0.4–1.4 of the maximum permissible concentration and can be used for treating natural and post-treating waste waters.     

Adsorption studies on copper,cadmium, and zinc ion removal from aqueous solution using magnetite/carbon nanocomposites

Magnetite/carbon nanocomposites were tested as adsorbents for removal of metal ions from aqueous solutions. The effect of adsorption parameters such as solutions pH (ranging between 2 and 9), the nature and the quantity of the sorbent (10, 20, 40, and 60 mg), initial concentration of metal ions (10, 30, 50, 100, and 150 mg/L), and temperature (25, 45, and 65°C) was evaluated. The removal efficiency of metal ions depends on solution pH and increases with increasing carbon content, the dose of magnetite/carbon nanocomposites, and the temperature and decrease with initial concentration of the metal ions. The adsorption kinetics was described by pseudo-second-order model, and the equilibrium experimental data were well fitted to the Sips isotherm, yielding a maximum adsorption capacity of 41.11, 76.67, and 48.45 mg/g for copper, cadmium, and zinc, respectively. The thermodynamic parameter Gibbs free energy was determined to be negative, which indicated that the adsorption process is spontaneous. The optimum conditions (1 g/L adsorbent, 25°C, and pH 6) were selected for removal of metal ions from real wastewaters, with good results indicating that investigated nanocomposites could be used for the application in real systems.     

斜发沸石对锌冶炼废水中钙镁离子的吸附研究

以去除锌冶炼废水中的钙镁离子、回收利用废水为目的,采用斜发沸石选择性吸附钙镁离子的方法,研究了不同改性剂对斜发沸石的改性效果,以及吸附时间、吸附温度、沸石粒径、搅拌强度等因素对吸附效果的影响。结果表明:用质量分数为15%的氢氧化钠改性沸石效果最佳,其对钙镁离子的吸附量与未活化的天然沸石相比可提高63%。活化改性沸石对钙镁离子的吸附量随吸附时间的延长开始增加得很快然后变慢,90 min基本达到饱和。升高吸附温度,活化改性沸石对钙镁离子的吸附量增加不明显;减小沸石粒径、增大搅拌强度,其吸附量显著增加,说明离子交换反应速率较快,内外扩散过程对吸附效果的影响较明显。静态等温吸附过程符合Langmiur吸附等温方程式。废水中其他离子的存在使吸附量降低26%。     

Adsorption isotherms,kinetic, and desorption studies on removal of toxic metal ions from aqueous solutions by polymeric adsorbent

Adsorption of Cd(II), Co(II), and Ni(II) on aminopyridine modified poly(styrene‐alt‐maleic anhydride) crosslinked by 1,2‐diaminoethane as an ion exchange resin has been investigated in aqueous solution. Adsorption behavior of these metal ions on the resin was studied by varying the parameters such as pH (2–6), adsorbent dose (0–4.0 g/L), contact time (0–240 min), and metal ions concentration (20–300 mg/L). Adsorption percentage was increased by increasing each of these parameters. The isotherm models such as: Langmuir, Freundlich, Temkin, and Dubinin–Radushkevich were used to describe adsorption equilibrium. The results showed that the best fit was achieved with the Langmuir isotherm equation, yielding maximum adsorption capacities of 81.30, 49.02, and 76.92 mg/g for Cd(II), Co(II), and Ni(II), respectively. The pseudo‐first‐order, pseudo‐second‐order, and intra‐particle diffusion kinetics equations were used for modeling of adsorption data and it was shown that pseudo‐second‐order kinetic equation could best describe the adsorption kinetics. The intra‐particle diffusion study revealed that external diffusion might be involved in this case. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41642.     

Absorption kinetics of ozone in aqueous O-cresol solutions

The kinetics of ortho-cresol ozonation in water has been studied using the film theory. At most experimental conditions investigated, the kinetic regime of the absorption has been fast pseudo first order with respect to ozone. At 30°C mass transfer plays an important role in controlling the absorption rate of ozone. The selfdecomposition reaction of ozone does not take place because of the great reactivity of o-cresol with ozone. Reaction rate constants have been determined at pH 7 for different temperatures below 30°C. The following Arrhenius equation was obtained: k_T = 2.2 × 10²³ exp(-11784/T), L/mol ? s At temperatures below 30°C there exists an ozone partial pressure value above which the ozone absorption kinetic regime changes to fast second order regardless of experimental conditions.