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Staining the Tubercle Organism in Sputum Smears 总被引:1,自引:0,他引:1
Mary A. Dabbow 《Biotechnic & histochemistry》1949,24(2):93-94
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Relative Susceptibility of Acid-Fast and Non-Acid-Fast Bacteria to Ultraviolet Light 总被引:5,自引:2,他引:3 下载免费PDF全文
F. M. Collins 《Applied microbiology》1971,21(3):411-413
Mycobacterium tuberculosis strain Erdman, M. bovis (BCG), and M. phlei showed a 1- to 2-log drop in viability after exposure to ultraviolet light compared to a 5-log drop over the same period for Staphylococcus albus, Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, and Serratia marcescens. L. monocytogenes showed an initial resistance to ultraviolet inactivation, but later the inactivation rate increased sharply. The significance of these findings with regard to the use of S. marcescens as a test organism for determining the bactericidal efficiency of ultraviolet lamps used to sterilize equipment contaminated with tubercle bacilli is discussed. 相似文献
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《Biotechnic & histochemistry》2013,88(5):265-268
Coupling a chromogenic amine to carboxyl groups of the lipids in the capsules of acid-fast bacteria was accomplished by reaction with benzidine, diazotization and using sodium beta naphthylate as the chromogen. By these means, acid-fast bacteria can be differentiated from non-acid-fast, and the intensity of the staining correlates well with the amount of lipid found in their capsules. By comparing the intensity of staining before and after treatment with 5% HCl, it is possible to demonstrate that some acidic capsular components are combined with calcium. Similarly, by comparing the intensity of staining before and after treatment with petroleum ether, the presence of oxyfatty acids (insoluble therein) can be demonstrated. 相似文献
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Coupling a chromogenic amine to carboxyl groups of the lipids in the capsules of acid-fast bacteria was accomplished by reaction with benzidine, diazotization and using sodium beta naphthylate as the chromogen. By these means, acid-fast bacteria can be differentiated from non-acid-fast, and the intensity of the staining correlates well with the amount of lipid found in their capsules. By comparing the intensity of staining before and after treatment with 5% HCl, it is possible to demonstrate that some acidic capsular components are combined with calcium. Similarly, by comparing the intensity of staining before and after treatment with petroleum ether, the presence of oxyfatty acids (insoluble therein) can be demonstrated. 相似文献
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Winsome Garvey Arleen Fathi Francine Bigelow Blair Carpenter Carmencita Jimenez 《Biotechnic & histochemistry》1986,61(4):251-253
Combined Gram techniques have been reviewed in the interest of improving technical safety and reliability in the demonstration of bacteria, particularly the Gram-negative type. The many modifications of the technique present various difficulties (Brown and Brenn 193 1, Humberstone 1963, Taylor 1966, Luna 1968, Brown and Hopps 1973, Engbaek et al. 1979, Bancroft and Stevens 1982, Churukian and Schenk 1982). 相似文献
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A medium containing tributyrin stabilized with polyvinyl alcohol was used to demonstrate the lipase activity of bacteria in foods. A modification of this new medium enables a search to be made for lipolytic bacteria in soil. 相似文献
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Tetsuya Matsumoto Mitsuo Kaku Kazuhiro Tateda Nobuhiko Furuya Yoichi Hirakata Keizo Yamaguchi 《Microbiology and immunology》1994,38(4):287-293
We evaluated antibody-coated bacteria (ACB) in expectorated sputum to discriminate contaminating or colonizing organisms from true pathogens. We examined 60 expectorated sputum samples from 51 patients with lower respiratory infections (chronic obstructive pulmonary disease 25, pneumonia 20, purulent tracheobronchitis 6). All samples were examined with quantitative culture and immunofluorescent demonstration of ACB. From the results of quantitative culture, we divided specimens into pathogen-isolated and pathogen-free samples. Among pathogen-isolated samples, in which we isolated accepted pathogenic organisms at ≥ 107 colony-forming units per ml, 16 of 23 samples were ACB-positive (69.5%). In contrast, among pathogen-free samples, in which we isolated accepted pathogens at < 107 colony forming units per ml or only upper respiratory flora, only 3 of 37 samples were ACB-positive (8.1%). The ACB-positive rate was significantly higher in pathogen-isolated than in pathogen-free samples (P < 0.001). Consequently, detecting ACB in expectorated sputum shows good potential as another criterion for distinguishing contaminating or colonizing organisms from true pathogens. 相似文献
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E. DeWitt Miller 《Biotechnic & histochemistry》1937,12(4):139-141
Cytoplasmic inclusions of various types of cells have been investigated by macerating or smearing and fixing and staining by different mitochondrial methods of technic. The results obtained as regards granular, rod-like, filamentous and globular forms immediately suggest a relation between these and similar cell inclusions which have in the past been described as mitochondria in certain cases of this material. While mechanical disturbance and drying before fixation apparently do not alter the staining properties of these forms, alcohol produces somewhat variable results depending upon the kind of material being investigated. Results indicate the presence in these smears of numerous intracellular bacteria, readily misinterpreted as mitochondria. In addition, there occur in certain cells, both in smears and sections, inclusions of indeterminate nature. 相似文献
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C. E. Dukes 《BMJ (Clinical research ed.)》1939,2(4111):799-801
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