标本保存时间对健康人外周血淋巴细胞亚群分析的影响

目的:观察样本保存时间对流式细胞术分析健康人外周血淋巴细胞亚群结果的影响.方法:随机选取健康人外周血标本23例,分别在采血后立即检测(D0组)、4℃保存1 d(D1组)、2 d(D2组)、3 d(D3组)和5 d(D5组)后用流式细胞仪分析淋巴细胞亚群.结果:D5组淋巴细胞的物理参数发生变化;D5组CD3+细胞百分比明...     

多发性骨髓瘤患者淋巴细胞亚群变化对免疫功能的影响研究

目的分析多发性骨髓瘤（MM）患者外周血T 淋巴细胞尧B 淋巴细胞和自然杀伤（NK）细胞的变化遥方法收集69 例 MM 患者及52 例健康体检人员的抗凝血,用流式细胞仪检测外周血淋巴细胞总数（Lym）尧CD3+ T 淋巴细胞尧CD19+ B 淋巴细 胞及CD16+56+NK细胞数量遥结果与对照组比较,MM 组Lym尧CD3+尧CD4+尧CD8+尧CD4+/CD8+尧CD19+等6个项目差异均有 统计学意义（约0.05）,CD16+56+差异无统计学意义（跃0.05）遥结论MM患者在疾病进程中与T淋巴细胞亚群及B淋巴细胞尧 Lym 密切相关,对评价MM 患者的免疫状况尧疾病进展和疗效等方面具有重要作用遥     

低剂量辐射对不同岗位男性放射人员外周血T淋巴细胞亚群的影响

目的:探讨低剂量辐射对不同岗位男性放射人员T淋巴细胞亚群水平的影响.方法:检测168例男性放射人员CD3+T、CD3+/CD4+T、CD3+/CD8+T、CD4+/CD45RA+T、CD4+/CD45RO+T、CD8+/CD45RA+T、CD8+/CD45RO+T、CD8+/CD28+T、CD8+/CD95+T、CD8...     

一种新的淋巴细胞亚群流式细胞检测方法的建立

目的 根据流式细胞仪的结构和原理,结合淋巴细胞亚群表面抗原的分布特点,设计新的流式细胞检测荧光抗体标记组合方案,以简化操作步骤,减少操作失误,降低试验成本.方法 在组合方案CD4-FITC/CD8-PE/CD3-PE-Cy5中加入CD19-FITC,则PE-Cy5和FITC双阳性细胞为CD4+T淋巴细胞,PE-Cy5阴...     

猪繁殖与呼吸综合征病毒感染仔猪淋巴细胞亚群的动态

目的：研究SPF仔猪感染猪繁殖与呼吸综合征病毒后对猪瘟苗的免疫应答受到抑制的机理。方法：利用流式细胞术检测了SPF仔猪感染PRRS病毒BJ-4后外周血淋巴细胞亚群的动态。结果：外周血CD3^ 、CD4^ 、CD8^ 和SLA-DR^ 表达细胞在感染早期比例下降;感染猪扁桃体的CD3^ 和CD4^ CD8^ 细胞亚群比例下降;肠系膜淋巴结的CD3^ 细胞亚群在感染后细胞在感染早期比例下降;感染猪扁桃体的CD3^ 和CD4^ CD8^ 细胞亚群比例下降;肠系膜淋巴结的CD3^ 细胞在感染后下降,但是SLA-DR^ 细胞亚群有逐渐升高的趋势。结论：仔猪感染PRRS病毒后淋巴细胞各亚群的比例下降可能会抑制机体对其它病原体的免疫反应,扁桃体的细胞比例变化有利于其它呼吸道病原体的混合感染或继发感染。     

流式细胞术检测肿瘤患者外周血T淋巴细胞亚群研究

目的 探讨肿瘤患者T淋巴细胞亚群的变化及临床意义。方法 采用流式细胞术检测27例肿瘤患者和25例正常对照组的T淋巴细胞亚群。结果 肿瘤患者CD3^＋T细胞、CD4^＋T细胞和CD4^＋／CD8^＋比值明显低于对照组并有统计学意义，而CD8^＋T细胞显著高于对照组（P〈0．01）。结论 肿瘤患者的免疫功能下降。流式细胞术对肿瘤患者的免疫功能的检测具有快速、敏感、准确的特点，对于评价疗效、判断预后具有重要价值。     

rIL—2对人脐带血淋巴细胞和成人外周血淋巴细胞亚群…

比较分析ｒＩＬ－２刺激对人脐带血和成人外周血淋巴细胞亚群及功能的影响。方法利用流式细胞技术对脐带血和成人外击血淋巴细胞亚群进行检测，用ｔ检验做统计学分析。结果新鲜末刺激的脐带血淋巴细胞在发育 和功能方面均比成人外击血幼雅；现款对ｒＩＬ－２刺激的反应明显不同，脐带血的变化主要表现为ＣＤ４＾＋和ＣＤ８＾＋细胞亚群的成熟     

乙型肝炎病毒感染者外周血T淋巴细胞亚群表达与其血清HBV-DNA水平的相关性分析

观察乙型肝炎病毒(HBV)感染者外周血T细胞亚群功能变化与其血清HBV-DNA水平,探讨病毒复制程度与细胞免疫功能的关系.应用流式细胞术直接荧光法检测142例HBV感染者和35名健康对照者外周血中T淋巴细胞亚群百分率,用荧光定量PCR法检测HBV感染者血清HBV-DNA.结果显示:与健康对照组比较,慢性乙型肝炎组CD4...     

应用四色荧光标记流式细胞术检测外周血淋巴细胞亚群的变化

目的 探讨四色荧光标记流式细胞术(Flow cytometry,FCM)检测鼻咽癌患者服用云芝丹参胶囊后外周血淋巴细胞亚群:辅助性T淋巴细胞(CD4 ),抑制性和细胞毒T淋巴细胞(CD8 ),B淋巴细胞(CD19 ),NK(CD16 CD56 ) 细胞的绝对数和百分率等的变化.方法 收集鼻咽癌患者27例,设立中药组和安慰剂对照组,采用四色荧光标记流式细胞术对服用云芝丹参胶囊后的鼻咽癌患者外周抗凝全血的淋巴细胞亚群CD4 细胞、CD8 细胞、B细胞、NK细胞进行绝对计数和相对计数,并对两组结果进行比较分析.结果 四色荧光标记流式细胞术结果显示:接受放射治疗的鼻咽癌患者在服用云芝丹参胶囊16周后,外周血T淋巴细胞的绝对数和百分率,以及Ts、Th细胞绝对数的下降均明显低于安慰剂组(P＜0.05);中药组和安慰剂组NK细胞百分率的变化均显著增高(P＜0.05); 但两组NK细胞绝对值的变化不存在明显差异(P＞0.05).结论 云芝丹参能明显减轻放射治疗对鼻咽癌患者的淋巴毒性.     

急性脾破裂患者手术前后外周血淋巴细胞亚群的变化及临床意义

目的:观察急性脾破裂患者在脾切除后外周血淋巴细胞亚群水平的改变。探讨短期内脾切除术对机体免疫功能的影响。方法:利用流式细胞仪测定74例急性脾破裂患者在手术前后淋巴细胞亚群水平。结果:急性脾破裂患者手术后CD 3T淋巴细胞及CD 4T淋巴细胞较手术前升高(P<0.01);CD(16 56)细胞(即NK细胞)和CD 19B淋巴细胞较手术前降低(P<0.05);而CD 8T淋巴细胞及CD 4/CD 8较手术前无明显差异(P>0.05)。结论:脾切除术后,短期内患者的免疫功能有改变。     

HIV/AIDS患者HAART后颈动脉内膜中层厚度的变化

目的 探讨HAART后HIV/AIDS患者颈总动脉分叉处内中膜厚度的变化并分析相关因素.方法 我们对40例初治患者和98例正在治疗的患者进行了横断面研究,根据患者的治疗时间将接受治疗的患者分为三组,通过多普勒超声检测颈总动脉分叉处内膜中层厚度、流式技术检测CD4+T细胞绝对计数,使用PCR检测血浆病毒载量,收集患者临床资料,如年龄、空腹血脂(包括总甘油三酯、总胆固醇、HDL、LDL)水平、D-二聚体检测值.使用SPSS 19.0对患者各项检查结果进行单因素方差分析.结果 四组患者性别年龄没有统计学差异,治疗前CD4+T细胞绝对计数之间的差异具有统计学意义(P＜0.05),且治疗越早的患者治疗前CD4+T细胞绝对计数越低.相比于未治疗的患者,治疗后患者的CD4+T细胞绝对计数、颈总动脉分叉处内膜中层厚度、胆固醇水平及HDL水平均升高,血浆病毒载量下降,且差异有统计学意义(P＜0.05).治疗后患者之间并无统计学差异.结论 HIV/AIDS患者HAART后颈动脉内中膜厚度增厚可能与血脂异常有关,而且可能发生在HAART的早期.     

高效抗病毒治疗促使艾滋病患者免疫功能重建

艾滋病的特征是ＨＩＶ 1感染人体后 ,造成ＣＤ4 +Ｔ淋巴细胞数量进行性减少、细胞免疫功能损害 ,最后导致艾滋病 (ＡＩＤＳ)。先前的研究表明这种免疫功能的丧失是不可逆转的 ,抗ＨＩＶ病毒治疗仅能控制或减缓其进展。近年来 ,由于强效联合抗病毒治疗 (ＨＡＡＲＴ)的应用 ,艾滋病的发病率和死亡率均较前明显下降 (指西方国家 )。说明ＨＡＡＲＴ不仅能有效的控制ＨＩＶ 1的复制 ,并能使艾滋病病人的免疫功能得到恢复。这种ＨＡＡＲＴ使艾滋病病人免疫功能重建的假说最近被一组法国研究人员证实 ,艾滋病的免疫重建规律是 :(1)治疗早期ＣＤ4 +…     

Kinetics of lymphocyte subsets from peripheral blood during a Plasmodium falciparum malaria attack.

Variations of lymphocyte subsets were followed longitudinally in 16 patients during an acute falciparum malaria attack. Before treatment, lymphocyte numbers were highly reduced, but the subset distribution was similar to that of healthy individuals. After parasite clearance, lymphocyte counts were normalized and the subset distribution was unchanged. This led to a normalization of all the absolute counts of lymphocyte subsets, except CD8+. The fast normalization of lymphocyte counts suggests that the initial decrease in lymphocyte numbers may reflect sequestration. Magnitude and kinetics of this variation were not related to parasite density or to severity of the attack. Activated T cells (CD3+HLA-DR+) were fewer in African than in European patients, suggesting the importance of the past exposure to malaria parasites in reallocation phenomena. These variations in lymphocyte numbers must be taken into account in the design and the analysis of cellular investigations in patients experiencing a falciparum malaria attack.     

自体外周血造血干细胞移植后淋巴细胞亚群的恢复

目的探讨自体外周血造血干细胞移植(auto-PBSCT)后免疫重建的规律。方法测定血液肿瘤患者auto-PBSCT前后淋巴细胞各亚群的绝对值。结果部分亚群在移植前低于正常。移植后各细胞亚群恢复时间不同,其中NK细胞在6个月恢复;B细胞在9个月恢复正常。T细胞总数在9个月恢复正常,其中主要为CD3 CD8 细胞。CD3 CD4 细胞在18个月恢复,而CD4 CD28 细胞在2年内仍未恢复。CD3 CD8 细胞在移植后早期有所下降,在3个月时恢复并高于正常,至9个月时逐渐下降至正常。CD3 CD8 细胞的主要部分是免疫表型呈活化的T细胞,即CD8 HLA-DR 细胞和CD8 CD38 细胞。两者分别在1个月和3个月时高于正常,在9个月时恢复正常。结论血液肿瘤患者移植前即存在淋巴细胞亚群异常的前提下,移植后NK细胞最先恢复,其次为B细胞,T细胞各亚群中CD3 CD8 细胞恢复较早,其中主要为活化T细胞。     

Opportunistic disorders of the gastrointestinal tract in the age of highly active antiretroviral therapy

Since the 1996 advent of highly active antiretroviral therapy (HAART) for the treatment of HIV/AIDS, the incidence of most opportunistic disorders in the developed world has dramatically declined but definitely has not disappeared. The number of new yearly HIV infections (about 55?000) and the total number of US infections (more than 1.1 million) remain very significant. Post-HAART gastrointestinal (GI) symptoms and biopsy results are still common, especially in large inner-city hospitals. The same opportunistic GI disorders were diagnosed in 442 endoscopies performed since 1996 as before, but at about one half the rate. The esophagus had the highest rate of positive biopsy results (46%), especially due to Candida. Helicobacter pylori infection has become the most common gastric infection. The small bowel still showed cytomegalovirus (CMV), cryptosporidia, and Mycobacterium avium complex (MAC) infections. In decreasing order, the most common large bowel infections were CMV, cryptosporidiosis, MAC, and spirochetosis. Cases of adenovirus, bacterial colitis, Kaposi sarcoma, and lymphoma were still diagnosed. Rectal biopsy specimens were the least productive. Microsporidiosis is now being diagnosed with special stains. Thus, where HIV/AIDS is common, it is important to be able to diagnose these GI processes. In addition to presenting post-HAART incidences, diagnostic features and aids are described for selected entities.     

类风湿性关节炎患者2H4+T淋巴细胞亚群的流式细胞仪分析

了解类风湿性关节炎患者免疫功能的异常变化，探讨其发病机制。方法，使用间接免疫荧光和直接免疫荧光方法对１１例ＲＡ患者和１２例健康人的外周血Ｔ淋巴细胞进行了研究。结果：ＲＡ患者与对照组比较ＣＤ３＾＋Ｔ细胞和ＣＤ＾＋４Ｔ细胞地明显变化，ＣＤ８＾＋Ｔ细胞明显减少，     

Effect of blood storage on lymphocyte subpopulations

In the evaluation of helper and suppressor T lymphocyte subpopulations testing of the cells may not be carried out until the day following collection of the blood. The possibility that changes occur during overnight storage at 4 degrees C or 22 degrees C has been investigated. No statistical differences in percentages of helper and suppressor T cells between fresh samples and overnight stored samples were found if the blood was kept at 22 degrees C.     

Prognostic value of phenotypic alterations in blood lymphocyte subsets in a murine retrovirus-induced immunodeficiency syndrome (MAIDS).

Mice infected with the Duplan strain of murine leukaemia virus (Dup MuLV), a retrovirus, develop a syndrome sharing several features with AIDS, including lymphadenopathy and profound immunodeficiency. We measured the changes in peripheral blood lymphocyte populations and evaluated their predictive value for the outcome of disease in C57Bl/6 mice. Animals were inoculated with Dup MuLV (SC1/Dup MuLV confluent fibroblast supernatant or spleen extract from an infected mouse). Peripheral blood lymphocyte subsets were sequentially monitored for 73 days using flow cytometric analysis and MoAbs directly conjugated to fluorochromes. A striking fall in the Thy1.2+ cell count occurred in diseased animals, mostly affecting the CD8+ cell compartment. At the same time, the percentage of Ly5+ cells was increased. Mice were killed at day 73 and spleen and lymph node lymphocytes were analysed. Phenotypic lymphocyte modifications in peripheral blood were closely related to those in the spleen or lymph nodes. Analysis of Ly6c antigen expression on CD4+ and CD8+ cells showed a selective expansion of the CD8+Ly6c+ subset, which may reflect a state of immune activation. Our results suggest that phenotypic alterations of peripheral blood lymphocytes are a good marker of disease progression in this model and could be a useful criterion to evaluate antiretroviral therapy.     

CD4+ T-lymphocyte nadir and the effect of highly active antiretroviral therapy on phenotypic and functional immune restoration in HIV-1 infection

To evaluate the effects of the timing of highly active antiretroviral therapy (HAART) on immune reconstitution, we compared lymphocyte subpopulations and lymphocyte proliferation (LP) in response to Candida albicans, cytomegalovirus, HIV p24, Mycobacterium avium complex, pokeweed mitogen, streptokinase, and tetanus toxoid in 43 patients with pretherapy advanced, moderately advanced, and early chronic HIV-1 infection. All patients had recent CD4⁺ T-cell counts >450/μl and HIV RNA <400 copies/ml for >12 months. CD4⁺ nadirs were positively correlated with recent numbers of CD4⁺ T-cells (P < 0.001), memory cells (P < 0.001), and na??ve CD4⁺ T-cells (P < 0.05) and CD4⁺ CD28⁺ T-lymphocytes (P < 0.05) and were negatively correlated with recent CD8⁺ T-lymphocyte counts (P < 0.05). Only CD4⁺ na??ve T-cells normalized when HAART was initiated at lower CD4⁺ T-cell levels. Fifty-three percent of patients had LP responses to HIV p24 antigen. While LP responses to prevalent antigens were usually present, responses to tetanus toxoid were more common with higher CD4⁺ T-lymphocyte nadirs (P < 0.05). Delaying HAART may limit phenotypic and functional immune restoration in HIV-1 infection.