Abstract A glucose oxidase-glucoamylase-bienzyme electrode has been developed and tested for determination of α-amylase activity. To eliminate interfering endogeneous glucose a glucose oxidase-catalase anti-interference layer was coupled with the bienzyme electrode. Linearity was obtained for the kinetic signal up to 1.0 I.U. α-amylase. Glucose was effectively eliminated up to 2 mM final concentration thus not influencing α-amylase determinations. A general concept for anti-interference enzyme layers is suggested. 相似文献
In this study, the gene encoding an α-amylase from a psychrophilic Arthrobacter agilis PAMC 27388 strain was cloned into a pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3). The recombinant α-amylase with a molecular mass of about 80 kDa was purified by using Ni2+-NTA affinity chromatography. This recombinant α-amylase exhibited optimal activity at pH 3.0 and 30 °C and was highly stable at varying temperatures (30–60 °C) and within the pH range of 4.0–8.0. Furthermore, α-amylase activity was enhanced in the presence of FeCl3 (1 mM) and β-mercaptoethanol (5 mM), while CoCl2 (1 mM), ammonium persulfate (5 mM), SDS (10 %), Triton X-100 (10 %), and urea (1 %) inhibited the enzymatic activity. Importantly, the presence of Ca2+ ions and phenylmethylsulfonyl fluoride (PMSF) did not affect enzymatic activity. Thin layer chromatography (TLC) analysis showed that recombinant A. agilis α-amylase hydrolyzed starch, maltotetraose, and maltotriose, producing maltose as the major end product. These results make recombinant A. agilis α-amylase an attractive potential candidate for industrial applications in the textile, paper, detergent, and pharmaceutical industries. 相似文献
The current study indicates that octyl-β-D-glucopyranoside (OGP) as a detergent which has the ability to make the lipid layer stiff. OGP was subjected for toxicity studies and in vitro cytotoxicty assays on cancerous HeLa and non-cancerous myoblasts H9c2 cell lines. Test against aquatic organisms were carried out in Artemia salina and LC50 values were calculated. Hemolytic activity tested for blood bio-compalibity showed hemolysis rate of 10–16%, followed by thrombolytic activity to burst the clots in blood. Also, the samples showed good lysis when compared to the standard streptokinase. Furthermore, α-amylase activity has been carried out to check the inhibition of α-amylase by the OGP. Finally, antibacterial activity has been tested against four different pathogens and their MIC values have been calculated. 相似文献
The inhibitory activity on pancreatic α-amylase by cyanidin-3-rutinoside was examined in vitro. The IC?? value of cyanidin-3-rutinoside against pancreatic α-amylase was 24.4 ± 0.1 μM. The kinetic analysis revealed that pancreatic α-amylase was inhibited by cyanidin-3-rutinoside in a non-competitive manner. The additive inhibition of a combination of cyanidin-3-rutinoside with acarbose against pancreatic α-amylase was also found. These results provide the first evidence for the effect of cyanidin-3-rutinoside in a retarded absorption of carbohydrates by inhibition of pancreatic α-amylase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus. 相似文献
Abstract Immobilization of α- and β-amylases on epoxypropylsilanized PartiSphere-5 was achieved. Hydrolysis of 2% potato starch solution yielded limit dextrin on α-amylase bound column while a mixture of limit dextrin, maltose and glucose was obtained from β-amylase bound column. The β-amylase bound column converted limit dextrin from α-amylase column into glucose. 相似文献
In this paper, we present a new colorimetric technique as a novel assay for the easy and direct detection of α-amylase activity. This detection system utilizes the interaction of α-amylase with starch that is supporting copper/gold (Cu/Au) nanoclusters. The Cu/Au nanoclusters are synthesized using starch as a stabilizing agent at room temperature. These nanoclusters show robust peroxidase-like activity and are able to catalyze the oxidation of TMB (3,3,5,5-tetramethylbenzidine) in the presence of hydrogen peroxide (H2O2), leading to the generation of a blue-colored solution. The α-amylase detection mechanism is based on the digestion of the starch by α-amylase, which results in nanocluster aggregation, leading to increased nanoparticle size and thus decreased peroxidase-like activity of the Cu/Au NCs. Experiments showed that the gradual addition of α-amylase causes the peroxidase activity to decrease step by step in a linear fashion. Using this method, colorimetric sensing of α-amylase was achieved with a detection limit (LOD) of 0.04 U/mL and a linear range of 0.1–10 U/mL. This method is significantly selective for α-amylase and could be affordably and conveniently applied to the detection of α-amylase in blood serum.
Two bacterial α-amylases from new industrial strains were studied: α-amylase fromBacillus amyloliquefaciens CCM 3502 (Czechoslovak) and thermostable α-amylase fromBacillus licheniformis 44MB82 (Bulgarian). The thermostable enzyme hydrolyzed starch mainly to dextrins, and after 1 h, 30% of the products were oligosaccharides. TheB. amyloliquefaciens enzyme produced more maltooligosaccharides than the first enzyme (B. licheniformis). Within 1 h, up to 80% of the substrate were hydrolyzed, giving different spectrum of oligosaccharides in comparison with the thermostable one. 相似文献
A platinum redox sensor for the direct potentiometric determination of α-amylase concentration has been described. The sensor measured the amount of triiodide released from a starch-triiodide complex, which was correlated with the α-amylase activity after biocatalytic starch degradation. The composition and stability of the potassium triiodide solution was optimized. The starch-triiodide complex was characterized potentiometrically at variable starch and triiodide concentrations. The response mechanism of the platinum redox sensor towards α-amylase was proposed and the appropriate theoretical model was elaborated. The results obtained using the redox sensor exhibited satisfactory accuracy and precision and good agreement with a standard spectrophotometric method and high-sensitive fully automated descret analyser method. The sensor was tested on pure α-amylase (EC 3.2.1.1, Fluka, Switzerland), industrial granulated α-amylase Duramyl 120 T and an industrial cogranulate of protease and α-amylase Everlase/Duramyl 8.0 T/60 T. The detection limit was found to be 1.944 mU for α-amylase in the range of 0-0.54 U (0-15 μg), 0.030 mKNU for Duramyl 120 T in the range of 0-9.6 mKNU (0-80 μg) and 0.032 mKNU for Everlase/Duramyl 8.0 T/60 T in the range of 0-9.24 mKNU (0-140 μg). 相似文献
The basal face of a silver iodide crystal in unsaturated water vapor is covered by a continuous molecular layer which serves as an underlying film. The structure of the film demonstrates long-range molecular order and looks like a honeycomb. Thus, macroscopic manifestations of the substrate wetting are due to the structure of the underlying film rather than the substrate crystal surface as such. A quarter of hydrogen bonds of the film molecules participate in bonding with the ions of the second crystallographic layer of the substrate. Three other quarters ensure the integrity of the film. The interactions with the ions of the first crystallographic layer are antibonding in nature. No free molecules serving as hydrogen bond donors are left on the film surface to keep vapor molecules. The shape of the free energy function associated with the adsorption of vapor molecules indicates its markedly layered nature. 相似文献