乳腺增生患者膻中穴体表红外辐射光谱探讨

目的：比较乳腺增生病患者膻中穴与非穴对照点体表红外辐射光谱的差异。方法：采用高灵敏度PHE201体表红外光谱仪，检测68例乳腺增生病患者膻中穴与非穴对照点体表1．5～16．0μm波段的红外辐射光谱。结果：乳腺增生病患者膻中穴与非穴对照点红外辐射光谱形态相似，但辐射强度减低，在检测的59个波长点中有13个波长检测点（6．75～8．25μm、9．00μm、9．25μm、9．75μm、13．25～13．75μm），膻中穴与非穴对照点的红外辐射强度比较差异有显著性意义（P〈0．05）。结论：膻中穴红外辐射强度降低。结合期门穴红外辐射强度等研究，提示乳腺增生病在病理状态下，不同的经脉系统处于不同的虚实状态。     

Efficacy of suspended moxibustion stimulating Shenshu(BL23)and Guanyuan(CV4) on the amygdala-HPA axis in rats with kidney-Yang deficiency symptom pattern induced by hydrocortisone

OBJECTIVE: To investigated the effects of suspended moxibustion stimulating Shenshu(BL23) and Guanyuan(CV4) acupoints on the amygdala and HPA axis in our rat model and elucidated the possible molecular mechanisms of moxibustion on kidney-Yang deficiency symptom pattern(KYDS). METHODS: Sixty male Sprague Dawley rats were randomly divided into a control group(n = 12) and an experimental group(n = 48). Rats in the experimental group were given intramuscular injections of hydrocortisone to establish...     

Study on Clinical Efficacy of Needling Danzhong（CV 17） in Treating Postpartum Hypogalactia

目的研究针刺膻中穴治疗产后缺乳的临床作用,为确定腧穴主治提供临床依据.方法采用多中心单盲随机对照研究方法,将276例乳汁不足的产妇随机分为针刺组与中药组,连续治疗3天,观察乳房充盈程度、泌乳量、泌乳素及新生儿体重、人工喂养次数和容量、婴儿小便次数、哭闹时间长短等指标,对照比较治疗产后缺乳的临床疗效.结果针刺组与中药组均能有效改善缺乳状态,乳房充盈程度、泌乳量及新生儿体重、人工喂养次数和容量、婴儿小便次数等治疗前后比较差异均有统计学意义,两组之间比较差异无统计学意义.针刺组治疗前后泌乳素无差异,中药组治疗前后泌乳素有差异.结论针刺膻中穴能有效促进乳汁的分泌.     

麦粒灸四花穴治疗反流性食管炎案

患者,女,48岁.初诊日期:2020年4月27日.主诉:反酸、烧心2月余.现病史:2个月前无明显诱因出现反酸、烧心,食后加重,伴有胸闷,无腹痛,大便溏薄,小便调.于浙江某医院诊断为"反流性食管炎",经抑酸、保护胃黏膜等治疗后(具体药物不详),病情无明显缓解.为求针灸治疗来浙江中医药大学附属第三医院针灸科就诊.刻下症:反...     

乳腺增生患者膻中穴体表红外辐射光谱探讨

OBJECTIVE: To compare differences of surface infrared radiation spectrums between Danzhong (CV 17) A high-sensitive PHE and non-acupoint control point in the patient of hyperplasia of mammary glands. METHODS: 201 surface infrared spectrograph was used to detect infrared radiation spectrums of Danzhong (CV 17) and non-acupoint control point at 1.5-16.0 microm wave band in the patient of hyperplasia of mammary glands. RESULTS: The shape of the infrared spectrums of Danzhong (CV 17) was similar to that of the non-acupoint control point, but with lower radiation intensity. Of the 59 wavelength spots detected, 13 (6.75-8.25 microm, 9.00 microm, 9.25 microm, 9.75 microm, 13.25-13.75 microm) had significant differences in infrared radiation intensity between Danzhong (CV 17) and non-acupoint control point (P<0.05). CONCLUSION: The intensity of infrared radition of Danzhong (CV 17) is lower and Qimen (LR 14) is higher than that of the non-acupoint control point in the patient with hyperplasia of mammary glands, showing that different channels are at different states of deficiency and excess under pathological condition of hyperplasia of mammary glands.     

乳块消方对实验性乳腺增生大鼠的影响

目的：研究乳块消方(RKX)对实验性乳腺增生大鼠的影响,并探讨其作用机制。方法：60只大鼠随机分为乳块消方5.8,11.6,23.2 g·kg^-13个剂量组,三苯氧胺组,模型组和正常组。采用肌注苯甲酸雌二醇联合黄体酮的方法复制乳腺增生大鼠模型,造模同时给予药物治疗。测定乳房直径和乳头高度、脏器系数、血清性激素水平、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及血液流变性,镜下观察各组大鼠乳腺病理形态学变化。结果：乳块消方能明显减小大鼠乳房直径、乳头高度和乳腺小叶数、小叶内腺泡数,此外,明显降低血清雌二醇(E2)、催乳素(PRL)水平,提高孕酮(P)水平,增加SOD活性,抑制胸腺系数减少。结论：乳块消方对大鼠乳腺增生病有良好的治疗作用。     

理气散结颗粒对大鼠乳腺增生的影响

目的研究理气散结颗粒对大鼠乳腺增生的影响。方法 96只大鼠随机分为空白对照组、模型对照组、他莫昔芬组(0. 003 3 g/kg)、小金丸组(0. 200 0 g/kg)、理气散结丸组(1. 957 0 g/kg)及理气散结颗粒低、中、高剂量组(0. 978 5、1. 957 0、3. 914 0 g/kg),每组12只,除空白对照组外各组大鼠肌内注射苯甲酸雌二醇和黄体酮以诱导乳腺增生。给药30 d后取血,ELISA法测定血清雌二醇(E2)、孕酮(P)水平,免疫组化法检测乳腺组织雌激素受体(ER)、孕激素受体(PR)蛋白表达,光镜下观察乳腺组织病理变化。结果与模型对照组比较,理气散结颗粒中剂量组ER蛋白表达显著降低(P0. 05);高剂量组E2水平显著升高(P0. 05),ER、PR蛋白表达显著降低(P0. 05,P0. 01);低剂量组组织增生明显改善(P0. 05)。结论理气散结颗粒对大鼠乳腺增生的影响可能与改善乳腺组织中ER、PR蛋白表达有关。     

Pathologic analysis on hyperplasia of mammary gland with different syndromes based on infrared radiation temperature of acupoints

Objective

To explore the pathologic characteristics of hyperplasia of the mammary gland (HMG) by observing differences in infrared radiation temperature of points of HMG in patients with different syndromes compared with healthy controls.

Methods

A FLIR Systems Therma CAM™ P30 infrared thermal camera was used to detect the infrared temperature of Shanzhong (CV 17), Qimen (LR 14), Zhongwan (CV 12), Qihai (CV 6), Guanyuan (CV 4), Taixi (KI 3), and Taichong (LR 3) in 113 patients with HMG. Of these patients, 71 were placed in the Liver Qi stagnation group, 34 were placed in the Dysfunction of conception and thoroughfare vessels group, and 8 were placed in the Phlegm and blood stasis in combination group. The infrared radiation temperature of each point in the patients was compared with that of healthy controls, and the differences in the infrared radiation temperatures of the points in the patients were analyzed.

Results

Overall, the bilateral corresponding point in both the controls and patients exhibited no significant difference in infrared radiation temperature. In all cases, the infrared radiation temperature of the points from proximal to distal tended to decrease. In a comparison of the patients and controls, the infrared radiation temperature of the trunk points Shanzhong (CV 17), Qimen (LR 14), Zhongwan (CV 12), Qihai (CV 6), and Guanyuan (CV 4) of the patients was higher than that of the controls, while the infrared radiation temperature of the lower extremity points Taixi (KI 3) and Taichong (LR 3) was lower than that of the controls. Of these points, Shanzhong (CV 17) (P=0.0368), Zhongwan (CV 12) (P=0.0028), Qihai (CV 6) (P=0.0085), and Guanyuan (CV 4) (P=0.0018) showed significant differences. In a comparison of the corresponding point on the same side in the Liver Qi stagnation group and controls, the infrared radiation temperature of Shanzhong (CV 17) (P=0.0089), right-side Qimen (LR 14) (P=0.0382), Zhongwan (CV 12) (P= 0.0000), Qihai (CV 6) (P=0.0011), and Guanyuan (CV 4) (P=0.0000) of the patients was significantly higher than that of the controls, while the differences in the infrared radiation temperature of the other points were not statistically significant (P= 0.0833–0.8397). In a comparison of the corresponding point on the same side in the Dysfunction of conception and thoroughfare vessels group and controls, the infrared radiation temperature of left-side Taichong (LR 3) (P=0.0048), right-side Taichong (LR 3) (P=0.0329), left-side Taixi (KI 3) (P= 0.0171), and right-side Taixi (KI 3) (t=0.544, P= 0.0165) of the patients was significantly lower than that of the controls, while the differences in the infrared radiation temperature of the other points were not statistically significant (P=0.3793–0.9197). In a comparison of the corresponding point on the same side in the Phlegm and blood stasis in combination group and controls, the infrared radiation temperature of Shanzhong (CV 17), Qimen (LR 14), Qihai (CV 6), Guanyuan (CV 4), Taixi (KI 3), Taichong (LR 3), and Zhongwan (CV 12) tended to increase, but without statistical significance (P=0.175-.759).

Conclusion

The corresponding points of HMG patients with different syndromes are in different deficiency/excess states. Changes in the infrared radiation temperature of the trunk points Shanzhong (CV 17), Qimen (LR 14), Zhongwan (CV 12), Qihai (CV 6), and Guanyuan (CV 4) are closely related to the pathological characteristics of the Liver Qi stagnation syndrome of HMG patients, while changes in the infrared radiation temperature of the lower extremity points Taixi (KI 3) and Taichong (LR 3) are closely related to the pathological characteristics of the Dysfunction of conception and thoroughfare vessels syndrome of HMG patients. On the whole, HMG patients with Liver Qi stagnation syndrome are characterized by “upper excess,” and those with Dysfunction of conception and thoroughfare vessels syndrome are characterized by “lower deficiency.”     

隔药灸神阙穴治疗原发性痛经疗效观察

目的:观察隔药灸神阙穴治疗原发性痛经的临床疗效。方法:将102例患者随机分为隔药灸组和西药组各51例,隔药灸组隔药灸神阙穴,西药组口服镇痛药进行治疗。结果:隔药灸组治愈17例,显效21例,有效9例,无效4例,总有效率为92.2%;西药组治愈7例,显效11例,有效21例,无效12例,总有效率76.5%。两组总有效率经比较差异具有统计学意义(P0.01),隔药灸组优于西药组。两组治疗前后痛经症状积分及两组不良反应比较,差异均具有统计学意义(均P0.01),隔药灸组均优于西药组。结论:隔药灸神阙穴治疗原发性痛经的临床疗效优于口服镇痛药,具有安全、不良反应少的优点,患者依从性好,值得推广应用。     

乳安消片对乳腺增生大鼠的治疗作用

目的观察乳安消片对乳腺增生大鼠的治疗作用。方法 60只大鼠随机分为空白对照组、模型组、乳癖消片组(960 mg/kg)及乳安消片低、中、高剂量组(580、1 160、1 740 mg/kg),每组6只,除空白对照组外各组大鼠后腿内侧肌内注射苯甲酸雌二醇和黄体酮以建立乳腺增生模型。灌胃给药45 d后,测定第2对乳头高度及乳房直径,ELISA检测血清雌二醇(E2)、催乳素(PRL)、孕酮(PROG)水平,HE染色进行组织病理学观察。结果与模型组比较,乳安消片组E2、PRL水平显著降低(P0. 05),PROG水平显著升高(P0. 05),并且中、高剂量组乳头高度及乳房直径显著减小(P0. 05)。结论乳安消片对乳腺增生大鼠具有明显的治疗作用,其机制可能与调节体内性激素水平有关。     

点刺肝俞、膏肓俞为主治疗肝郁痰凝型乳腺增生病临床观察

目的:观察三棱针点刺肝俞、膏肓俞穴为主,对肝郁痰凝型乳腺增生病患者的临床疗效。方法:将确诊为肝郁痰凝型乳腺增生病患者118例随机分为两组,治疗组78例,采用三棱针点刺肝俞、肾俞穴0.1~0.2寸,随后在两穴处用闪火法拔罐出血,若伴乳房胀痛加刺臂中穴,痰盛者加刺丰隆穴。对照组40例予以口服乳癖消片治疗。结果:两组疗效比较,治疗组有效率明显优于对照组(P<0.05);两组即时止痛效果比较,治疗组即时止痛效果明显优于对照组(P<0.01)。结论:三棱针点刺肝俞、膏肓俞穴为主对肝郁痰凝型乳腺增生病患者症状改善有明显作用。     

乳络通胶囊对大鼠乳腺增生模型的影响

目的:观察乳络通胶囊(乳络通)对实验性大鼠乳腺增生病的影响,探讨其作用机制。方法:Wistar大鼠随机分为6组:正常组、模型组、乳康片(0.53g/kg)和乳络通高、中、低剂量组(0.60g/kg、0.30g/kg、0.15g/kg)。以肌注苯甲酸雌二醇及黄体酮的方法复制大鼠乳腺增生模型,测量血清中激素水平(E2、LH、PRL、FSH、P),光镜下观察乳腺形态学变化及用免疫组化方法观察乳腺组织中雌激素受体(ER)和孕激素受体(PR)的变化。结果:与模型组比较,乳络通0.60g/kg、0.30g/kg剂量组能显著改善动物体内激素水平,使它们接近正常水平;乳络通0.60g/kg、0.30g/kg剂量组能够使大鼠乳腺增生模型乳腺组织中的ER、PR的阳性表达水平显著或者明显降低,显著抑制乳腺小叶增生。结论:乳络通通过调整激素水平,降低实验大鼠乳腺组织ER、PR含量,抑制乳腺小叶增生而起到抑制乳腺增生的作用。     

艾盐包热熨膻中穴治疗产后郁滞期乳痈患者的效果观察

[目的] 探讨艾盐包热熨膻中穴治疗产后郁滞期乳痈患者的效果。[方法] 选取148例产后郁滞期乳痈患者,分为观察组和对照组,每组74例。对照组给予常规护理;观察组予加用艾盐包热熨膻中穴。比较两组症状积分、临床疗效、血清肿瘤坏死因子-α（TNF-α）水平、血清C反应蛋白（CRP）水平。[结果] 相较于对照组,观察组治疗后乳房肿块、乳房胀痛、乳汁分泌不畅积分明显降低（P<0.05）,且观察组治疗总有效率显著增高（P<0.05）。观察组血清TNF-α、CRP水平较对照组显著降低（P<0.05）。[结论] 艾盐包热熨膻中穴治疗产后郁滞期乳痈,可显著改善临床症状,提高临床疗效,抑制血清炎症因子水平可能是其主要机制。     

肝舒乐片对乳腺增生病大鼠性激素受体的影响

目的：通过动物实验观察肝舒乐片对乳腺增生病大鼠性激素受体的影响，探讨其对乳腺增生病的防治作用。方法：以雌、孕激素注射大鼠右下肢肌肉内建立乳腺增生病模型。将72只大鼠随机分为正常对照组、模型对照组、乳癖消组、肝舒乐组、疏肝组和健脾组6组，每组12只，运用免疫组化测定法测定各组大鼠雌激素受体（ER）、孕激素受体（PR）含量变化。结果：模型组ER、PR的表达均明显高于正常组，（P〈0.05）。肝舒乐片组、乳癖消组雌、孕激素受体（PR）的表达明显降低，趋于正常（P〈0．05），但两组间无显著性差异（P〉0．05）。疏肝组、健脾组ER、PR的表达均无明显改善。结论：肝舒乐片对模型动物的ER、PR含量紊乱有显著调节作用。     

Clinical Application of Moxibustion on Guanyuan （CV 4）

Guanyuan （CV 4） is located 3 cun below the umbilicus, on the midline of abdomen. It is the Front-Mu Point of the Small Intestine, a crossing point of Three Yin Meridians of Foot and Conception Vessel. It can warm the kidney to invigorate yang, tonify the Yuan-Primordial qi, dredge and regulate Thoroughfare and Conception Vessels,     

Effect of electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) on the expression of EphB2 protein in cortex around cerebral infracted area of rat

Objective

To observe the effect of electroacupuncture (EA) on the expression of erythropoie-tin-producing hepatocyte receptor B2 (EphB2) in the cortex around the infracted area of middle cerebral artery occlusion (MCAO) rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia.

Methods

A total of 180 male Sprague-Dawley (SD) rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups: postoperative 3 d, postoperative 14 d and postoperative 21 d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride (TTC) staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators.

Results

The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group (P<0.05). The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells (EphB2⁺) around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group (P<0.05), and the positive cell number was higher than that in the sham operation group (P<0.01). Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2⁺ and postsynaptic density-95 positive (PSD-95+) were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group (P<0.05).

Conclusion

Electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.

     

艾灸"肺俞""膏肓"对BLMA5诱导大鼠肺纤维化的影响

目的:研究艾灸"肺俞""膏肓"对博莱霉素所致大鼠肺纤维化的阻抑作用,为针灸介入肺纤维化防治提供实验依据.方法:实验用SD大鼠分为4组:对照组、模型组、艾灸组、泼尼松组.对照组气管内注入生理盐水,其余3组气管内注入博莱霉素制作大鼠肺纤维化模型.测定分析各组治疗后第7、14、28 d肺组织病理学变化以及比较各组第28 d肺系数.结果:艾灸组及泼尼松组肺系数明显减少(P<0.01),肺组织病理学显示肺泡炎及肺纤维化程度均明显减轻.结论:艾灸"肺俞""膏肓"对肺间质纤维化具有一定的防治作用.     

Effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV14) on transdermal uptake of sinapine thiocyanate in asthma gel

OBJECTIVE: To investigate the effect of stimulating the acupoints Feishu(BL 13) and Dazhui(GV 14) on the transdermal uptake of sinapine thiocyanate contained in a gel used for the management of asthma.METHODS: Thirty Sprague-Dawley rats were randomly divided into three equal groups using a random number table: the Feishu(BL 13) acupoint group, the Dazhui(GV 14) acupoint group, and the nonacupoint group or control group. Using micro-dialysis technology, preprocessed skin probes were implanted into the rats at Feishu(BL 13), Dazhui(GV 14), and a nonacupoint site. Asthma gel was then placed on the skin at Feishu(BL 13), Dazhui(GV14) acupoints, and the nonacupoint for all groups.Dialysate was collected every 30 min for 12 h. The normalized concentration of sinapine thiocyanate in the skin was determined by high-performance liquid chromatography.RESULTS: The rat in vivo transdermal experiment demonstrated that the quantity-time equation showed a good linear correlation with zero-order kinetics(r 0.99). The transdermal behavior was in accordance with the first-order rate open model in which the transdermal penetration rates and the accumulative amounts of sinapine thiocyanate in the skin at the acupoint sites were greater than those through the skin of the nonacupoint site. The systemic maximum concentration and the area under the curve of sinapine thiocyanate in the acupoint groups were significantly greater than in the nonacupoint group. A lag time was observed in both acupoint groups, but not in the nonacupoint group.CONCLUSION: Stimulating the acupoints promotes the percutaneous absorption of sinapine thiocyanate and also controls its release, reducing concentration fluctuations in the blood.     

Effects of moxibustion and acupuncture at Zusanli(ST 36) and Zhongwan(CV 12) on chronic atrophic gastritis in rats

OBJECTIVE: To evaluate the effects of moxibustion and acupuncture of Zusanli(ST 36) and Zhongwan(CV 12) acupoints on chronic atrophic gastritis(CAG) in rats, and to study the mechanisms behind their actions.METHODS: Forty-four male Sprague-Dawley rats were induced with CAG by intragastric administration of 40% ethanol combined with free drinking of N-methyl-N′-nitro-N-nitrosoguanidine and irregular feeding for 12 weeks, followed by daily treatment with moxibustion or acupuncture for 2 weeks. His...     

电针肝俞、足三里对抑郁型功能性消化不良大鼠脑肠相互作用的研究

目的观察电针"肝俞""足三里"对抑郁型功能性消化不良大鼠抑郁状态、胃肠动力及脑内相关蛋白表达的影响,探讨电针治疗抑郁型功能性消化不良的机制。方法将48只雌性Wistar大鼠随机分为空白组、模型组、电针组和非穴组,每组12只。应用慢性不可预见性温和应激法建立抑郁型功能性消化不良大鼠模型。于造模第15日开始,电针组每日予电针"肝俞""足三里"干预,非穴组给予距尾尖1/3、2/3处电针刺激,干预14 d。于实验前1 d和实验第14、28日对各组大鼠进行体质量检测、旷场实验和蔗糖偏好实验;于取材前对各组大鼠进行黑色糊状物灌胃,40 min后检测大鼠胃内残留率和小肠推进率;Western blot检测大鼠外侧缰核βCa MKⅡ、海马脑源性神经营养因子(BDNF)蛋白的表达。结果与空白组比较,模型组和非穴组大鼠体质量、蔗糖偏好率、行为学评分、小肠推进率、海马BDNF表达明显下降(P0.01),胃内残留率和外侧缰核βCa MKⅡ表达明显升高(P0.01);与模型组比较,电针组大鼠体质量、蔗糖偏好率、行为学评分、小肠推进率、海马BDNF表达明显升高(P0.01),胃内残留率和外侧缰核βCa MKⅡ表达明显降低(P0.01);与非穴组比较,电针组大鼠外侧缰核βCa MKⅡ表达量显著降低、海马组织BDNF表达量显著增加(P0.01)。结论电针"肝俞""足三里"可改善抑郁型功能性消化不良大鼠的抑郁状态和胃肠动力。