microRNA-107在肝癌中的表达及临床意义

目的检测microRNA-107（miR-107）在肝癌中的表达及意义,并探讨其对肝癌细胞增殖能力的影响。方法利用公共基因 表达数据库（TCGA数据库和GEO数据库）中的肝癌microRNA表达谱数据库,分析miR-107在肝癌及癌旁的表达水平;收集22 例肝癌及其相应癌旁新鲜组织标本,qRT-PCR方法检测miR-107的表达水平;收集53例石蜡包埋的肝癌组织标本,qRT-PCR方 法检测miR-107表达水平并分析其在肝癌中的临床意义。在人肝癌细胞株Huh7中过表达或沉默miR-107,通过MTT方法检测 其对肝癌细胞增殖能力的影响。结果miR-107在肝癌组织中的表达显著高于其在癌旁组织中的表达,差异具有统计学意义 （P<0.05）。在肝癌组织中,miR-107与肿瘤大小呈正相关关系（P=0.032）。体外实验结果表明,过表达miR-107后肝癌细胞增殖 能力明显增强（P<0.0001）,而沉默miR-107后细胞增殖能力明显降低（P<0.0001）。结论miR-107在肝癌中表达上调,其高表达 与肝癌细胞增殖密切相关,miR-107可能是一个与肝癌的发生发展相关的基因。     

PTEN在乳腺癌中的表达及其与细胞增殖活性的关系

目的:探讨PTEN在乳腺癌组织中的表达及其与乳腺癌细胞增殖活性的相关性.方法:用免疫组化方法对60例乳腺癌和18例乳腺增生症的石蜡包埋标本进行PTEN、PCNA的检测,其中PCNA指数作为衡量乳腺癌细胞增殖活性的参数.结合病人的临床病理资料进行统计分析.结果:PTEN在乳腺癌中的高表达率明显低于乳腺增生症(P＜0.05);PTEN的表达与乳腺癌细胞增殖活性显著相关;PTEN的表达水平与腋淋巴结转移、远处转移、ER水平和5年生存率相关.结论:PTEN可作为一个潜在的判断乳腺癌预后的指标.     

MCM2在膀胱移性细胞癌的表达及其预后意义

目的:探讨MCM2在膀胱移性细胞癌的表达及其预后意义.方法:采用免疫组织化学方法检测MCM2在36例膀胱移性细胞癌标本中的表达,同时分析MCM2表达与临床病理之间的关系,MCM2表达与肿瘤复发之间的关系.结果:36例膀胱移性细胞癌标本均表达MCM2,且其表达与肿瘤的分化程度有显著相关性(P＜0.001),而于临床分期无显著相关性,复发患者均为MCM2高表达.结论:MCM2在膀胱移性细胞癌组织中表达增高,MCM2表达标记指数可作为预后指标.     

肾母细胞瘤P53基因的免疫组化研究

为了解Ｐ５３基因在肾母细胞瘤中的表达，采用ＡＢＣ免疫组织化学方法，对５６例肾母细胞瘤石蜡包埋的标本进行检测。结果：５６例肾母细胞瘤标本中１３例Ｐ５３蛋白表达阳性，预后不良组织类型Ｐ５３蛋白是性率，显著高于预后好级组织类型；有转移肿瘤Ｐ５３蛋白表达阳性率显著高于无转移肿瘤。     

不同XAGE-1异构体在肝细胞癌中的表达

目的 探讨肝癌细胞株HepG2、SMMC-7721以及肝癌患者肿瘤组织标本中的4种XAGE-1异构体的表达情况,同时研究其与肝癌患者临床病理之间的关系.方法 使用RT-PCR方法检测肝癌细胞株HepG2、SMMC-7721以及80例肝癌患者肿瘤组织标本中的4种XAGE-1异构体的表达情况,同时收集患者临床资料,对XAGE-1b的表达与肝癌患者临床病理特征之间的关系进行研究.结果 XAGE-1b和XAGE-1d mRNA在肝癌细胞株和部分患者肿瘤组织标本中阳性表达[分别是35/80(43.75%)、6/80(7.50%)];而XAGE-1a和xAGE-1c mRNA在肝癌细胞株和肝癌患者标本中均为阴性表达;在对照组组织标本(4例肝血管瘤,8例肝破裂)中4种xAGE-1异构体均为阴性表达.XAGE-1bmRNA在肝癌组织中具有较高的表达率,并且其表达与肝癌TNM分期相关.结论 XAGE-1b在肝癌细胞中呈阳性表达,同时在肝癌组织中呈高度阳性表达,预示XAGE-1b的表达与肝癌预后相关,可作为其免疫治疗的潜在靶点之一.     

Syndecan—1在胆囊癌中的表达及其临床意义

目的 探讨Syndecan-1蛋白在胆囊癌中的表达及其临床意义.方法 采用免疫组织化学(SP)法检测45例胆囊癌石蜡标本和26例胆囊炎石蜡标本中Syndecan-1蛋白的表达.结果 Syndecan-1在胆囊癌组织中的表达较胆囊炎组织明显降低(P<0.05),Syndecan-1的表达与胆囊癌病理分级及肿瘤转移有显著相关性(p<0.05),但与患者年龄,有无伴发结石差异无统计学意义(P>0.05).结论Syndecan-1的异常表达可能与胆囊癌的发生、发展有关,检测syndecan-1可评估胆囊癌预后.     

肝癌患者肿瘤坏死因子-α和白细胞介素-8水平变化及其与临床病理特征的关系

目的探讨肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)在肝癌中联合检测的临床意义,揭示二者变化与肝癌临床病理特征的关系。方法采集诊断明确的肝癌患者(肝癌组)术前、术后血清与健康人群(对照组)血清,并采集肝癌患者肝组织石蜡标本,采用酶联免疫吸附法检测血清TNF-α和IL-8水平,免疫组织化学法检测肝癌患者肝组织中TNF-α和IL-8的表达,分析其与临床病理特征的关系。结果肝癌患者术后血清TNF-α和IL-8水平较术前显著降低(P<0.01),但仍显著高于对照组人群(P<0.01)。肝癌患者血清TNF-α和IL-8水平与性别、年龄、组织类型、临床分期及肿瘤大小无明显相关性(P>0.05),与有无淋巴结转移有关(P<0.05)。肝癌组织中TNF-α和IL-8的表达呈正相关(P<0.01)。结论 TNF-α和IL-8可作为肝癌转移的重要标志物及预后评估的指标。     

BRD4在宫颈鳞状细胞癌中的表达及其机制研究

目的探讨溴结构蛋白4(BRD4)在宫颈鳞状细胞癌的表达特征及对宫颈鳞癌细胞增殖的影响及作用机制。方法用免疫组织化学染色法检测72例宫颈鳞癌患者手术切除后石蜡包埋标本和配对癌旁正常宫颈组织中BRD4的表达情况,所有病例均选自2015—2017年南阳市中心医院病理科石蜡标本。结果 BRD4蛋白在宫颈鳞癌细胞中表达阳性率显著高于正常宫颈鳞状上皮细胞。BRD4的表达与宫颈鳞癌的分化程度显著相关。结论 BRD4的表达与宫颈鳞癌疾病发生、发展明确相关,是潜在预后评估标记物,因而可能对宫颈鳞癌的检测及诊疗有重要意义。     

着丝粒蛋白U在肝细胞癌中的表达及其临床意义

目的 观察着丝粒蛋白U（CENPU）在肝细胞癌（HCC）组织中的表达,探索其与患者预后及临床病理特征之间的相关性。方法 从癌症基因组图谱（TCGA）数据库收集HCC数据集,下载CENPU mRNA表达谱及预后资料,比较CENPU mRNA在50例正常肝组织与371例HCC组织中的表达,分析mRNA表达量与患者预后相关性。收集2010~2012年兰州大学第一医院80例HCC患者石蜡组织标本,采用免疫组织化学方法检测癌组织中CENPU的蛋白表达水平,并分析其与临床病理特征之间的相关性。结果 TCGA数据库数据分析揭示了CENPU高表达患者的预后不良（P=0.025）。免疫组织化学结果显示,HCC组织中CENPU蛋白可作为肝癌患者的独立预后因子（P=0.023）。结论 CENPU在HCC中的高表达,其可能参与肝细胞的癌变及进展,可能是HCC患者的一个不良预后因素。     

肝癌组织中脯氨酸羟化酶3 (PHD3)的差异表达对预测原发性肝癌切除术后预后的价值

 目的  检测脯氨酸羟化酶3 (prolyl hydroxylase 3,PHD3)在肝癌及癌旁组织中的表达情况,探讨其对原发性肝癌切除术后患者预后的预测价值。方法  收集本院2006年进行肝切除术后病理确诊为肝细胞肝癌的标本325例,制作成组织芯片。采用免疫组化法检测肝癌及癌旁肝组织中PHD3的表达水平,并比较PHD3表达水平与肝癌患者无瘤生存时间及总体生存时间的相关性。应用Kaplan-Meier法、χ2检验及t检验分析PHD3的预测价值及与临床病理参数的关系。在325例患者组织标本中随机选取无复发转移和复发转移患者各6例的肝癌组织标本,提取组织蛋白进行Western blot检测。结果  免疫组化显示,肝癌细胞中PHD3主要在胞质表达,核内也有表达。癌组织中PHD3的表达水平与与肝癌患者的生存预后显著正相关,与患者性别、微血管侵犯、肿瘤大小和甲胎蛋白不相关。癌旁组织中PHD3的表达与预后无显著相关。Western blot 检测发现6例无复发患者的PHD3蛋白表达明显高于复发患者。结论  肝癌组织中PHD3的表达可以有效预测原发性肝癌切除术后患者的预后。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.