共查询到20条相似文献,搜索用时 875 毫秒
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目的 建立结肠靶向滴丸克癌素的提取工艺方法。方法 采用碱溶酸沉法从槐米中提取芦丁,再进行酸水解得到槲皮素;采用传统乙醇回流法从姜黄中提取总姜黄素,以姜黄中的总姜黄素含量为指标,通过正交表分析确定最佳提取工艺。结果 槲皮素的提取条件为:碱性pH 8~9,酸性pH 3~5条件下提取芦丁;得到精制芦丁加100倍量的2%硫酸酸水解90 min。姜黄的最佳提取工艺为:85%乙醇回流提取3次,每次1 h;得浸膏上硅胶柱收集氯仿洗脱部分得总姜黄素。结论 该工艺可行,可为结肠靶向滴丸克癌素的生产提供参考。 相似文献
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目的优选肩周炎痛贴中姜黄药材醇提工艺。方法以姜黄素的含量为考察指标,采用L9(34)正交试验考察乙醇浓度、乙醇用量、提取次数、提取时间4个因素,以确定最佳提取工艺。结果最佳提取工艺为姜黄加8倍量80%的乙醇回流提取3次,每次1h。结论该优化工艺操作简单,结果稳定,适用于工业生产。 相似文献
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从槐米中提取槲皮素的研究 总被引:1,自引:0,他引:1
目的 本文从槐米中提取分离槲皮素进行研究.方法 采用碱溶酸沉法提取,再进行酸水解得到槲皮素.结果 确定了碱溶解酸沉淀法提取槐米中槲皮素的最佳条件及槲皮素的结构鉴定.结论 用碱溶解酸沉淀法从槐米中提取槲皮素是很有意义的,可以较好的提取分离出较高纯度的槲皮素. 相似文献
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以槐角为原料制备染料木素的工艺研究 总被引:1,自引:0,他引:1
目的制定一种以槐角为原料,适用于工业化生产制备染料木素的最佳工艺。方法以槐角为原料,采取正交实验,确定最佳的槐角苷提取工艺;在单因素考察的基础上,采取正交实验,确定槐角苷水解得到染料木素的最佳条件。结果槐角中槐角苷的最佳提取工艺是:80%乙醇、煎煮120 min、8倍量、提取3次;槐角苷最佳酸解工艺是:用2.5 mol.L-1盐酸乙醇液酸解,料液比为1∶15,酸解温度为90℃,酸解时间为2.5 h。结论该方法经济适用,是可用于大批量生产的制备方法。 相似文献
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Dong Liu Joshua Schwimer Eugene A. Woltering Frank L. Greenway 《Pharmaceutical biology》2013,51(10-11):677-682
Curcumin isolated from turmeric (Curcuma longa L., Zingaberaceae) root was found to be anti-angiogenic in a human tissue-based angiogenesis assay. As a liposoluble compound, curcumin can be extracted from turmeric root with organic solvents such as ethanol or acetone. Curcumin in its pure form has poor solubility in water, potentially limiting its medicinal use for humans when it is taken orally or injected. This study attempted to investigate the possibility of improving curcumin's low solubility using an extract as a carrier. This would maintain anti-angiogenic properties with improved water-solubility. Experiments were undertaken to determine the extraction efficiency of different solvents for curcumin. Anti-angiogenic activities of curcumin in its pure form and in extracts were compared as a general trend ethanol or acetone was more efficient in extracting curcumin than their aqueous counterparts. Using 50 and 70% aqueous ethanol as well as 70% aqueous acetone yielded significantly more turmeric extracts by weight than absolute acetone, which was the lowest. Conversely, turmeric extracts extracted with 95% ethanol and absolute acetone contained significantly higher curcumin concentrations than water extract, which was the lowest. Combining the higher extract yield and highest curcumin concentrations in the extract, 95% ethanol gave the highest yield of single entity curcumin. In the angiogenesis assay, pure curcumin at the concentration of 85 μ M (in 1% ethanol v/v) in the culture medium totally suppressed angiogenic responses. In contrast, a curcumin concentration of 18.5 μ M (in the form of 100 μ g/ml turmeric extract) achieved the same total inhibition of angiogenesis in culture. This nearly 5 fold gap reflected the unaccounted involvement of other antiangiogenic compounds including curcumin derivatives, and/or enhancement of curcumin by non-antiangiogenic compounds in the extract. This finding suggests that curcumin in the form of extracts be potentially more pharmacologically active than pure curcumin. Further investigations of this hypothesis and possible interactions are warranted. 相似文献
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鹅不食草总有机酸的提取与分析 总被引:1,自引:0,他引:1
目的优化鹅不食草总有机酸提取方法并测定其含量。方法分别采用超声提取法、碱提酸沉法、酸沉后有机溶剂萃取法、有机溶剂加酸水直接提取法等多种方法提取鹅不食草总有机酸,并用分光光度法测定总有机酸含量。结果碱提酸沉法为鹅不食草总有机酸最佳提取方法,总有机酸含量为67.3%。结论碱提酸沉法提取鹅不食草总有机酸的方法可行,分光光度法测定总有机酸含量简便、灵敏。 相似文献
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Betancor-Fernández A Pérez-Gálvez A Sies H Stahl W 《The Journal of pharmacy and pharmacology》2003,55(7):981-986
Pharmaceutical preparations derived from natural sources such as vegetables often contain compounds that contribute to the antioxidant defence system and apparently play a role in the protection against degenerative diseases. In the present study, commercial preparations containing extracts of turmeric, artichoke, devil's claw and garlic or salmon oil were investigated. The products were divided into fractions of different polarity, and their antioxidant activity was determined using the Trolox equivalent antioxidant capacity (TEAC) assay. This test is based on the efficacy of the test material to scavenge 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) derived radicals. Total phenols were determined in all fractions as well as specific carotenoids in the most lipophilic fraction to assess their contribution to the antioxidant activity. For comparison, the radical scavenging effect of selected constituents of the extracts such as curcumin, luteolin, kaempferol, chlorogenic acid, harpagoside, beta-carotene and alpha-tocopherol was investigated and compared with that of Trolox. Curcumin, luteolin, kaempferol, chlorogenic acid and beta-carotene showed an antioxidant activity superior to Trolox in the TEAC assay; harpagoside was barely active. All fractions of the turmeric extract preparation exhibited pronounced antioxidant activity, which was assigned to the presence of curcumin and other polyphenols. The antioxidant activity corresponding to the artichoke leaf extract was higher in the aqueous fractions than in the lipophilic fractions. Similarly, devil's claw extract was particularly rich in water-soluble antioxidants. Harpagoside, a major compound in devil's claw, did not contribute significantly to its antioxidant activity. The antioxidant capacity of the garlic preparation was poor in the TEAC assay. That of salmon oil was mainly attributed to vitamin E, which is added to the product for stabilization. In all test preparations, the antioxidant activity was significantly correlated with the content of total phenolic compounds. 相似文献
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仿生化提取人参皂苷类成分的初步研究 总被引:2,自引:0,他引:2
目的:对人参中的皂苷类成分进行仿生化提取。方法:以人参超微粉为原料,分别以仿生溶媒和水作为提取溶剂提取人参皂苷类成分。采用紫外-可见分光光度法测定人参总皂苷的含量;高效液相色谱法测定人参皂苷Rg1和人参皂苷Re含量之和,并分析色谱图特征;以成分的提取率为指标比较仿生化和水提取法的优劣。结果:仿生化提取人参总皂苷的提取率为61.31%,人参皂苷Rg1和人参皂苷Re的总提取率为62.63%;水提取人参总皂苷的提取率为54.26%,人参皂苷Rg1和人参皂苷Re的总提取率为43.09%。结论:仿生化提取法对人参总皂苷、人参皂苷Rg1和人参皂苷Re的提取效率高于水提取法,且仿生化提取物色谱图中显示有新成分产生。 相似文献
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Context: Aflatoxins as potent mycotoxins can influence vital parameters in chickens. Turmeric was used in decreasing toxic effect of mycotoxins on vital organs, traditionally.Objective: The study compared the protective effect of turmeric and MycoadTR in broilers exposed to aflatoxin.Materials and methods: Chickens (270) were divided into six groups. The chickens were fed a basal diet, turmeric extract (5?mg/kg diet), MycoadTR (25?mg/kg diet), productive aflatoxin (3?mg/kg diet), aflatoxin plus turmeric extract (3 versus 5?mg/kg diet), and aflatoxin plus MycoadTR (3 versus 25?mg/kg diet) in basal diet. At 28?d old, we determined plasma concentration of total protein, albumin, triglyceride, cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), calcium, potassium, phosphorous, uric acid, aspartate transferase (AST), and alanine aminotransferase (ALT). Furthermore, liver and kidney were sampled for pathological examination.Results: Chickens fed turmeric with aflatoxin had significant lower ALT, AST, and uric acid than chickens fed aflatoxin (11.4?±?0.79, 228?±?9, and 6?±?0.4 versus 17.2?±?1.7, 283?±?5, and 7.7?±?0.1) whereas, total protein, calcium, and HDL values in chickens fed aflatoxin plus turmeric increased significantly (2.66?±?0.16, 8.4?±?0.2, and 920?±?4.1 versus 1.7?±?0.17, 7?±?0.2, and 690?±?4.8). Pathological examination revealed severe congestion, degeneration, and necrosis in liver and kidney in chickens that received aflatoxin.Discussion and conclusion: The study showed that turmeric may provide protection against the toxic effects of aflatoxin on liver and kidney. 相似文献
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This study was carried out to see the hepatobiliary clearance of 99m Tc-Mebrofenin radiopharmaceutical in D-galactosamine induced hepatic rats. Furthermore, protective effect of turmeric extract has been studied in these hepatitis rats. Hepatitis was induced with intraperitoneal injection of D-galactosamine (400 mg/kg b. wt) in these rats. 1% turmeric extract was given along with their normal diet for 15 days. Turmeric extract treatment significantly increased the hepatic uptake of radioactivity and accelerated the excretion of 99m Tc-Mebrofenin as compared to control rats. (P < 0.001). In D-galactosamine administered rats, a significant delay was observed in 99m Tc-Mebrofenin excretion as compared to controls. However, D-galactosamine administered rats, pretreated with turmeric extract or concurrently treated with turmeric extract showed a near normal pattern of 99m Tc-Mebrofenin excretion. Hence, it can be suggested that turmeric extract may improve the liver function by detoxification. 相似文献
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Context: Protection of neurons from degeneration is an important preventive strategy for dementia. Much of the dementia pathology implicates oxidative stress pathways. Turmeric (Curcuma longa L.) contains curcuminoids which has anti-oxidative and neuro-protective effects. These effects are considered to be similar to those of citicoline which has been regularly used as one of standard medications for dementia.Objective: This study aimed at investigating the effects of turmeric rhizome extract on the hippocampus of trimethyltin (TMT)-treated Sprague-Dawley rats.Materials and methods: The rats were divided randomly into six groups, i.e., a normal control group (N); Sn group, which was given TMT chloride; Sn-Cit group, which was treated with citicoline and TMT chloride; and three Sn-TE groups, which were treated with three different dosages of turmeric rhizome extract and TMT chloride. Morris water maze test was carried out to examine the spatial memory. The estimated total number of CA1 and CA2–CA3 pyramidal cells was calculated using a stereological method.Results: The administration of turmeric extract at a dose of 200?mg/kg bw has been shown to prevent the deficits in the spatial memory performance and partially inhibit the reduction of the number of CA2–CA3 regions pyramidal neurons.Discussion: TMT-induced neurotoxic damage seemed to be mediated by the generation of reactive oxygen species and reactive nitrogen species. Turmeric extract might act as anti inflammatory as well as anti-oxidant agent.Conclusions: The effects of turmeric extract at a dose of 200?mg/kg bw seem to be comparable to those of citicoline. 相似文献